1.COMPARISON OF PRELIMINARY DETECTION RESULTS OF SEVERAL TOXOPLASMA GONDII ELISA TEST KITS
Anou NIU ; Youreng FENG ; Wenqi LIU
Chinese Journal of Zoonoses 2000;(4):56-58
Aim Five types of civil Toxoplasma gondii ELISA test kit were used to the detected specimen which proved to be the same, foreign-made test kit was used to examine the positive specimen given by these five types of test kit, as to make comparison about quality of test kit. Results IgG-ELISA A,B kits positive showing rates (sensitive)are all 60% ,the corresponding rate between A&B is merely 50% ,the specifics are 35%&90%. C, D,E kits' positive showing rates (sensitive)are 30 %, 60 %, 70 % the specifice are 75 %, 54 %, 87 %. The result about examining the probes given by A, B, D, E kit :IgG corresponding rates are better, which are all more than 90% ;IgM corresponding rates are all more than 60%. It turn out that, according to the civil conditions of ELISA test kit, we suggest that besides the quality being improved in, all kinds of test kit should be used combinatively, so as to avoid neglect and mistake on examination, and we should be cautious in examining.
2.Infuluence of electroacupuncture on blood lipid and liver function in nonalcoholic fatty liver rats
Wenqi FENG ; Zhihua ZENG ; Lianshi ZHUO
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(06):-
Objective:To investigate the infuluence of electroacupuncture on blood lipid and liver function in nonclcoholic fatty liver rats. Methods:Thirty-three male SD rats were randomly divided into 2 groups:normal control group (n=11,fed with normal food) and non-alcoholic fatty liver model group (NAFLD) (n=23,fed with high-fat food). Eight weeks later 1 rats were randomly selected from the normal control group and 2 rats were randomly selected from the NAFLD group and were killed to undergo pathological examination of the liver. When the establishment of experimental model of NAFLD rats was confi rmed the remaining 20 NAFLD rats were sublishment into 2 equal subgroups:NAFLD control group to be fed continuously with high-fat food ,acupuncture treatment group (to fed continuously with high-fat food). Take Zusanli,Hong Leong,Sanyinjiao,Taichong points,electroacupuncture -spacing parameters,2Hz frequency,intensity 4mV to the appropriate vibrating slightly lower limb. By the end of the 12th week,all rats were killed to isolate the samples of test the levels of serum total cholesterol (TC),serum triglyceride (TG),high-density lipoprotein cholesterol (HDL-L),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST),and liver pathology. Results:Treatment of rats --serum total cholesterol (TC),serum triglyceride (TG),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST) were significantly lower than those of NAFLD control group (P
3.Electroacupuncture treats rat non-alcoholic fatty liver by blocking insulin resistance and peroxidation
Zhihua ZENG ; Minghui ZENG ; Wenqi FENG ; Lianshi ZHUO
Journal of Third Military Medical University 2003;0(11):-
Objective To investigate the mechanism of electroacupuncture in the treatment of non-alcoholic fatty liver disease(NAFLD).Methods Thirty-three male SD rats were randomized into 2 groups:normal control group(n=11,fed with normal food)and experimental rats(n=23,fed with high-fat food).Eight weeks later,3 rats were randomly selected from the 2 groups respectively and killed to undergo pathological examination of the liver.When the establishment of experimental rats was confirmed,the remaining 20 experimental rats were divided into 2 equal subgroups:NAFLD group(fed continuously with high-fat food)and electroacupuncture treatment group(fed continuously with high-fat food and received the treatment 15 min per day,once a day for 4 weeks).Then all rats were killed to detect the serum levels of fasting blood glucose(FBG),fasting insulin(FINS)and free fatty acids(FFA),liver contents of malondialdehyde(MDA),glycerin tripalmitate(TG)and total cholesterol(TC),and the activities of liver superoxide dismutase(SOD)and glutathione(GSH).Liver pathological changes was examined.Results Compared with NAFLD group,electroacupuncture reduced the serum levels of FBG,FINS and FFA(P
4.Effects of transforming growth factorβ1 and its receptorβ2 gene single nucleotide polymorphisms on risk of intracranial hemorrhage in patients with brain arteriovenous malformation
Nan JIANG ; Xuesong LI ; Liangcan XIAO ; Wenqi HUANG ; Tiewei QI ; Shaolei GUO ; Feng LIANG ; Zhengsong HUANG
Chinese Journal of Anesthesiology 2012;32(4):485-487
Objective T0 investigate the effects of transforming growth factorβ1 ( TGFβ1 ) and its receptorβ2 (TGFβR2) gene single nucleotide polymorphisms on the risk of intracranial hemorrhage in patients with brain arteriovenous malformation (BAVM).Methods Fifty-three BAVM patients of both sexes aged 18-64 yr who were genetically unrelated native HAN of Guangdong province were divided into 2 groups:patients with and without intracranial hemorrhage ( n =30:23).Venous blood samples were collected and anti-coagulated with ethylene diaminetetraacetic acid for genomic DNA extraction.TGFβ1-509C/T (rs1800469) and TGFβR2 875A/G (rs3087465) gene SNPs were genotyped by using PCR-RFLP.Results There were no significant differences in genotype and frequency between the 2 groups.The G carrier frequency of the TGFβR2 genotype was significantly higher in patients with intracranial hemorrhage than in patients without intracranial hemonrhage.The G carrier of the TGFβR2 genotype was associated with intrarcranial hemorrhage in patients with BAVM.Conclusion TGFβ1 gene polymorphism is not relevant to the intracranial hemorrhage in patients with BAVM,but polymorphisms of TGFβR2 could be a risk factor.
5.Comparative study of therapeutic efficacy of systemic therapy with FOLFOX-6 and hepatic arterial infusion for hepatic metastases from colorectal cancer
Jianyong NIU ; Yonghong SUN ; Yi FENG ; Wenkai CHANG ; Shenghuai HOU ; Yaoping LI ; Wenqi BAI ; Xiaobo LIANG
Chinese Journal of Hepatobiliary Surgery 2010;16(6):422-427
Objective To compare the efficacy and side effects between systemic chemotherapy and hepatic arterial infusion by combination of oxaliplatin and 5-fluorouracil (FOLFOX-6) with 5-fluorouracil in the patients who have developed hepatic metastasis after colorectal cancer operation. The factors that would affect the prognosis without operational treatment were also analyzed. Methods 46patients who had signed the informed consents were allocated into two groups: the group with general chemotherapy (Trial Group includes 26 cases) and the one with hepatic arterial infusion chemotherapy (Control Group includes 20 cases). The total effective rate, the prognosis, the cytoxicitic side effects,quality of life, the total survival rate and the responses were the main parameters determined. Kaplan-Meier was used to analyze Mono-factor to the prognostic responses and the Cox mode was used to analyze poly-factor to the prognostic responses. Results The overall survival rate was significantly higher by using systemic treatment versus HAI(median, 15. 0 v 11.2 months;P<0.05). The difference in overall responsive rate (CR+PR) between the two groups was statistically significant (50% v 10%;P=0. 011). No significant difference was found in PS scale during the treatment. (P=0. 126). Except for myelosuppression and abdominal pain, no significant difference was found in the other side effects. Univariate analysis revealed that the invasive lesions to serosa, the distribution of liver metastases, the size and number of liver metastases, primary carcinoma involving lymph nodes and the treatment were correlated with prognoses. Cox regression analysis showed that the larger diameter of liver metastases, the number of liver lesions, primary carcinomas involved in serosal layer and the treatment modules were independent prognostic factors. Conclusions The oxaliplatin-based FOLFOX-6 chemotherapy regiment has a better responsive rate and survival rate than the traditional infusion with 5-fluorouracil to the main hepatic artery for interventional therapy. The diameter of the hepatic metastasis larger than 5em, multiple hepatic metastasis and the primary lesions penetrating serosal layer suggest the poor prognosis. The oxaliplatin-based systematic chemotherapy has a better prognosis. Therefore,it is worth carrying on further study on modification of traditional hepatic arterial infusion and on evaluation of therapy by combination of the hepatic arterial infusion with the systematic chemotherapy.
6.Apoptosis induced by DNA primase inhibitor 3,3'-diethyl-9-methylthia-carbocyanine iodide in human leukemia HL-60 cells
Zhiming LI ; Wenqi JIANG ; Zhongzhen GUAN ; Xiaofeng ZHU ; Junmin ZHOU ; Bingfen XIE ; Gongkan FENG ; Zhenyu ZHU ; Zongchao LIU
Acta Pharmaceutica Sinica 2006;41(10):978-984
Aim To investigate apoptosis induced by 3,3'-diethyl-9-methylthia-carbocyanine iodide(DMTCCI) , an inhibitor of DNA primase found in our previous study, and the mechanism of DMTCCI in human myelogenous leukemia HL-60 cells. Methods HL-60 cells were cultured in RPMI-1640 medium and treated with different concentrations of DMTCCI. MTT assay was used to detect growth inhibition.Flow cytometry and DNA ladders were used to detect apoptosis. Western blotting was used to observe the expression of survivin, Bcl-xL, Bad, Bax, Bcl-2, caspase-9, caspase-3, caspase-6, PARP, DFF45 and lamin B protein. Caspase-3 activity was measured by ApoAlert Caspase-3 Assay Kit. Results DMTCCI inhibited proliferation of human leukemia HL-60 cells with IC50 value of 0. 24 μmol · L-1. The results of flow cytometry and DNA ladders showed that DMTCCI could induce apoptosis of HL-60 cells. The expression levels of protein survivin and Bcl-xL were down-regulated, Bad and Bax were up-regulated,while Bcl-2 protein had no change in response to DMTCCI treatment in HL-60 cells. Treatment of HL-60cells with DMTCCI induced the proteolytic cleavage of caspase-9, caspase-3, caspase-6, PARP, DFF45and lamin B protein. Caspase-3 activity apparently increased at 3 h and reached a peak at 12 h after exposure to 1 μmol · L-1 of DMTCCI in HL-60 cells. Conclusion DMTCCI inhibited proliferation and induced apoptosis of human leukemia HL-60 cells. Bcl-2 family proteins, survivin and caspases family proteins might playa role in the apoptosis process induced by DMTCCI.
7.Detection of serum procalcitonin to guide second-classed AECOPD patients to use actibiotics in clinical application value
Tong WANG ; Xiaoqian CHEN ; Mengde ZHU ; Yubing WU ; Feng GAO ; Rui HAN ; Lingli HAO ; Wenqi LIU ; Xiu LI
The Journal of Practical Medicine 2014;(18):2908-2910
Objective To explore the clinical value of serum procalcitonin (PCT)-based antibiotic therapy in the second-classedexacerbations of chronic obstructive pulmonary disease (AECOPD). Methods 240 patients diagnosised as AECOPD were randomized to the PCT group and the control group. Serum PCT levels of patients from the PCT group were measured 1 h after hospitalized and the third, fifth, eighth day respectively. When PCT < 0.1 μg / L, patients will stop taking antibiotics and initiated while PCT≥0.1 μg / L. Antibiotic treatment in the control group was based on guidelines of COPD diagnosis and treatment. Results Duration of antibiotic therapy and hospitalization were respectively 5.6 ± 1.4 and 8.2 ± 1.1 days in the PCT group, 9.2 ± 2.2 and 11.4 ± 2.5 days in the control group (both P < 0.05). Mean costs of hospitalization expensesand antibiotic therapy were 5700 ± 201 and 1650 ± 189) yuan in the PCT group, 6210 ± 220 and 2350 ± 210 yuan in the control group (both P < 0.05). The clinical effective rate, times of exacerbation, one-year ΔFEV1, the 1-year hospitalization rate and time to next exacerbation all showed no significant differences between the two groups. Conclusion PCT-guided antibiotic treatment reduces antibiotic use inthe second-classed acute exacerbations patients.
8.Toxic effects of permethrin on HMC3 microglia and its associated mechanism
Wanli ZHANG ; Wenqi SHAN ; Chao CHEN ; Haowei DONG ; Hao YUAN ; Qiuming ZHOU ; Feng TAO ; Heng PENG ; Yajun MA
Journal of Environmental and Occupational Medicine 2024;41(3):267-275
Background Permethrin is a commonly used pyrethroid insecticide and has been found to be potentially neurotoxic. Microglia are innate immune cells in the central nervous system and are involved in the development of a range of neurodegenerative diseases. Objective To observe possible toxic effects of permethrin on human microglia clone 3 (HMC3) in vitro and explore associated mechanism. Methods HMC3 were treated with 0, 10, 25, and 55 μmol·L−1 permethrin for 72 h. Cell cycle and apoptosis were measured using flow cytometry. Cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase inhibitor 1A (CDKN1A), cyclin B2 (CCNB2), cellular tumor antigen p53 (p53), factor-related apoptosis (FAS), caspase 3 (CASP3), and H2A histone family member X (H2AX) were detected by quantitative real-time PCR (qPCR). The differential genes and enrichment pathways of HMC3 after 0 and 25 μmol·L−1 permethrin treatment was analyzed by RNA sequencing. HMC3 was treated by 0, 10, 25, and 55 μmol· L−1 permethrin for 72 h. The content of nitric oxide (NO) in the supernatant was detected using Griess reagent. The secretion level of interleukin-6 (IL-6) was detected by enzyme linked immunosorbent assay (ELISA). The mRNA expression levels of mitogen-activated protein kinase (MAPK) pathway (including MAPK1, MAPK8, and MAPK14), interleukin-1β (IL-1β), IL-6, and matrix metalloproteinase (MMP) families (including MMP1, MMP2, MMP3, and MMP9) were detected by qPCR. The protein expressions of phosphorylated p38 mitogen-activated protein kinase (p-p38), phosphorylated extracellular signal-regulated kinase (p-ERK), IL-1β, IL-6, and MMP1 were detected by Western blot. Results HMC3 was arrested in G2/M phase after 0, 10, 25, and 55 μmol·L−1 permethrin treatment for 72 h, of which there was a statistically significant difference between the 55 μmol·L−1 permethrin treatment group and the control group (P<0.01), and the mRNA expression of CDKN1A was up-regulated according to the qPCR (P<0.05). There was no statistically significant difference in the proportions of apoptosis between the groups (P>0.05). The RNA sequencing showed that the differential genes were enriched in the MAPK pathway, and the mRNA expressions of MAPK1, MAPK8, and MAPK14 were up-regulated after the permethrin treatment at 55 μmol·L−1 compared to the control group by qPCR (P<0.05). The Western blot revealed that, compared to the control group, the levels of p-p38 and p-ERK were increased after the 10 μmol·L−1 permetrin treatment (P<0.05), the p-ERK level was increased after the 25 μmol·L−1 permetrin treatment (P<0.05), and the p-p38 level was up-regulated after the 55 μmol·L−1 permetrin treatment (P<0.05). The secretion of NO in the supernatant of HMC3 increased after permetrin treatment compared to the control group (P<0.05), the mRNA and protein expressions and the secretion of IL-6 showed an upward trend, the mRNA and protein expressions of IL-1β were up-regulated (P<0.05), and the mRNA and protein expressions of MMP1 were up-regulated in the 25 and 55 μmol·L−1 permethrin groups (P<0.05). Conclusion Permethrin inhibits HMC3 cell proliferation in vitro, induces cell cycle arrest, activates MAPK pathway, and promotes the expression of inflammatory factors IL-1β and MMP1, which may be one of the mechanism of neurotoxicity induced by permethrin.
9.A cross-sectional survey on the types of antiviral treatment plans for patients with chronic hepatitis B
Xiulan XUE ; Qianguo MAO ; Qingfa RUAN ; Feng MIN ; Wenqi HUANG ; Xiaoting ZHENG ; Huiqing LIANG
Chinese Journal of Hepatology 2024;32(1):29-34
Objective:To understand the current antiviral treatment status and various clinical types of treatment plans in Xiamen City so as to explore ways to improve and optimize the diagnosis and treatment standards for chronic hepatitis B.Methods:A cross-sectional survey method was used to study the antiviral treatment status and treatment plans for chronic hepatitis B patients who visited and were diagnosed in the Department of Infectious Diseases and Hepatology of all tertiary hospitals in Xiamen City at 0:00~23:59 on May 25, 2022.Results:A total of 665 cases were surveyed in this study, with an antiviral treatment rate of 81.2%(540/665). The antiviral treatment rate of patients who accorded with the current guidelines for antiviral treatment indications was 85.8%(507/591). The antiviral treatment rate for 362 outpatients was 72.9%(264/362). Among them, the antiviral treatment rates were 80.1%, 89.3%, and 25.0%(226/282, 25/28, 13/52), respectively, for patients diagnosed with chronic hepatitis B, hepatitis B cirrhosis, and hepatitis B surface antigen-carrying status. The treatment plan for all outpatient patients was mainly oral nucleos(t)ide analogues, accounting for 59.1%(214/362). The antiviral treatment rate for 303 inpatients was 91.1%(276/303). The various clinical types of antiviral therapy rates among all patients were 70%~95%. The antiviral treatment plan for inpatients was mainly based on pegylated interferon alpha treatment, accounting for 72.6%(220/303).Conclusion:Antiviral treatment for chronic hepatitis B in Xiamen City can still be strengthened to meet the current demand for expanding antiviral treatment indications. Antiviral treatment rates and various types of treatment plans differ between outpatients and inpatients; thus, further awareness and acceptance of the goal of improving antiviral therapy, especially in outpatients, and the possibility for a clinical cure based on pegylated interferon alpha treatment are needed to maximize the benefit to more patients.
10.Practical research on scientific research training of medical laboratory undergraduates during medical internship
Qiliang LI ; Ziyao LI ; Jinghong FENG ; Dongzhi WANG ; Wenqi SONG
Chinese Journal of Medical Education Research 2023;22(4):559-563
In order to optimize the undergraduate teaching methods and improve students' comprehensive competitiveness, this study explored the scientific research training methods of medical laboratory undergraduates during medical internship. On the premise of ensuring that students carry out clinical practice according to the internship plan, the research group leads students to carry out scientific research training in their spare time. The scientific research training was divided into two stages. In the first stage, on the basis of informed consent and independent choice, the students in the control group were trained by self-regulated learning and teachers' question answering, while the students in the experimental group were trained by the way of centralized scientific research lectures and scientific research practice. In the second stage, all the students were in independent research and exploration under the guidance of teachers within 5 months. The results showed that in the process of independent research, the time of topic selection in the experimental group [(3.5±1.1) days] was significantly shorter than that in the control group [( 5.4 ± 1.9) days], and the time of topic design in the experimental group [(12.2±2.5) days] was significantly shorter than that in the control group [(14.6±3.1) days]. It shows that carrying out scientific research training in the medical internship stage of undergraduates is helpful to increase the efficiency of students' later independent research and accelerate the process of independent research.