Objective To investigate the effects of docosahexaenoic acid (DHA) on action potential (AP)and transient outward potassium channel (Ito) in rat ventricular myocytes in order to evaluate the anti-arrhythmia mechanism of DHA. Method The rat ventricular myocytes were isolated by using enzyme digestion method. AP and Ito of individual ventricular myocyte were recorded by using patch-clamp technique in whole-cell configuration at room temperature. The effects of DHA on AP and Ito were observed when it was applied in 0 μmol/L, 20 μmol/L, 40 μmol/L, 60 μmol/L, 80 μmol/L, 100 μmol/L and 120μmol/L separaterly. Results The 25%,50% and 90% of action potential duration (APD25, APD50 and APD90) were gradually prolonged with the escalation of concentration of DHA ( P ＜ 0.05, n= 20). The effects of DHA of different concentrations on AP maximal velocity (Vmax), AP amplitude (APA) and AP overshoot (OS) did not produce significantly different results (P ＞ 0.05, n= 20). The degree of blockade of Ito was concentration-dependent as different concentrations of DHA were applied, and as the concentration of DHA was escalated, the I-V curves went downwards, the stably inactivated curves shifted to the lift, and the time taken for recovery from inactivation prolonged ( P ＜ 0.05, n =20). However, the different concentrations of DHA did not produce different effects on stably activated curves ( P＞ 0.05). The Itos were blocked to (2.61 ± 0.26)%, (21.79±4.85)%, (63.11 ± 6.57)%, 75.52±7.26 ) %, (81.82 ± 7.63) % and (84.33 ± 8.25) % by the above given concentrations of DHA respectively under given potential equaling to + 70 mV( P ＜ 0.05, n = 20), and the half-effect concentration (EC50) of DHA on Ito was(49.11±2.68) umol/L. Conclusions The effects of DHA on APD and Ito may be one of the anti-arrhythmia mechanism of DHA.

Objective To explore the effect of bee-needle therapy on treatment of patients with rheumatoid arthritis.Methods Seventy five patients with rheumatoid arthritis were randomly divided into bee-needle group,drug group and bee-needle plus drug group.Therapeutic effect was compared.Results After treatment and follow up,there were significant differences in degree of joint pain,the number of swollen joints,tender joint count,morning stiffness time,the number of pain joints,HAQ,ESR,RF,and CRP among three groups (P ＜ 0.05).Conclusion Bee-needle therapy can significantly relieve the clinical symptoms of patients with rheumatoid arthritis.

Objective To explore the effect of bee-needle therapy on treatment of patients with rheumatoid arthritis.Methods Seventy five patients with rheumatoid arthritis were randomly divided into bee-needle group,drug group and bee-needle plus drug group.Therapeutic effect was compared.Results After treatment and follow up,there were significant differences in degree of joint pain,the number of swollen joints,tender joint count,morning stiffness time,the number of pain joints,HAQ,ESR,RF,and CRP among three groups (P ＜ 0.05).Conclusion Bee-needle therapy can significantly relieve the clinical symptoms of patients with rheumatoid arthritis.