Objective To assess the status of self-esteem of college students of higher vocational medical school and to provide targeted guidance.Methods Totally 1014 college students of higher vocational medical school were assessed using self-esteem scale and the results were analyzed by SPSS 13.0.Results The self-esteem levels among different student levels were different.The self-esteem level was lower in students of technical secondary school than in students of vocational college and junior college.The self-esteem levels were different among factors of different grades,family location,duty and post,satisfaction degree for school,individual health and family education (P ＜ 0.05 ) ;while they are not significantly different among factors of gender,major,being singleton or not and family types ( P ＞ 0.05 ).Conclusions School should further strengthen the mental health education and use kinds of methods to improve the self-esteem of college students.