Objective To identify the characteristics of Candida utilis uricase and that of Bacillus fastidious intra-cellular uricase.Methods The new strains were cultivated to prepare the uricase.Then the uricase was purified by ammonium sulfate fractionation precipitation,DEAE-cellulose 52 chromatography and preparative PAGE.The activity,characterization and subunit constitution were determined with the purified uricase.Results The two uricas-es were the iso-tetramer.The single peptide chain molecular weight and total molecular weight of Candida utilis uricase were 33.0 ku and 134.0 ku individually,the optimum pH was close to 8.8 and more than 50% activity was reserved at pH 7.4,the Michaelis-Menten constant was (32.8 ± 3.1) μmol/L (n = 10) and the inhibition constant of xanthine was (4.8 ± 0.2) μmol/L(n = 3) for this uricase.For Bacillus fastidious intracellular uricase,its single peptide chain molecular weight and total molecular weight were 35.7 ku and 151.0 ku,the optimum pH was over 9.0 and less than 30% activity left at pH 7.4,the Michaelis-Menten constant and the inhibition constant of xanthine were (204 ± 14) μmol/L(n = 8) and (41 ± 7) μmol/L(n = 5) individually for it.Conclusion Through the research,a significant theoretical basis was funded for constructing hybrid medicinal uricase to treat diseases as-sociated with hyperuricemia.

Throughputs of 802.11g wireless LAN in different distances and different modes were measured,as well as the real throughputs of medical images using PACS software.Main problems and the promising future of using 802.11g wireless LAN in PACS are discussed.

DICOM acquisition gateway,the strobe of PACS date flow,is a key element of PACS.But on the process of acquisition,the images sometimes might be lost.This paper will focus on the image recovery scheme of DICOM acquisition gateuay in the notion of image missing.

Objective To solve the new problems for quality assurance of PACS which imported by the use of PACS.Methods The medical imaging quality control system used between imaging modality and image acquisition gateway was designed.Results The medical imaging quality control system could partially solve the problem for quality assurance of PACS.Conclusion With the use of medical image quality control system,wrong or low quality image can be found and modified earlier.Therefore,the veracity of medical information and medical image of PACS can be improved farther.

Objective To develop plaque assay for SARS virus, in order to provide a reliable means for SARS research. Methods SARS virus BJ 01 strain in various dilutions was inoculated to Vero E6 cells, the cells were then covered with nutritious agar. The titers of the plaque were measured by Dullbecco R method. Results The plaque of SARS virus appeared on the third day after the cell cultures infected with virus. The plaque was round in shape, 2.5-3 mm in diameter. Conclusion The plaque assay developed in present study was stable and regular, and it could be used in SARS research.

Objective To investigate the protective effect of Yulangsan polysacharide ( YLSPS) and mechanism against ibuprofen-induced liver injury in mice. Methods The mice were randomly divided into the blank control(NC), the model control,YLSPS at 150 mg·kg-1 , 300 mg·kg-1 ,600 mg·kg-1 groups and biphenyldicarboxylate (150 mg·kg-1 BPDC) group. The mice were orally administered with corresponding agents once per day for consecutive 14 days, whereas the blank control group and model control group were orally administered with saline. Except the blank control group, all the other mice were orally administered IBU 200 mg·kg-1 body weight 2 h after last lavagedof medicimes. The mice were fasted and watered ad lib for 20 h after model establishment. Activities of ALT,AST and ALP,content of T-BiL,TNF-α,IL-6 in serum;activities of SOD,GSH-Px and content of MDA in liver tissue were detected. The morphological pathology test was used to examine degrees of hepatic injury. Results Compared with the model control, YLSPS could obviously reduce activities of ALT,AST and ALP,content of T-BiL, MDA,TNF-α and IL-6, and increase SOD,GSH-Px and CAT (P<0. 05), and then lessen the hepatic injury. Conclusion YLSPS showed potential protective effect against ibuprofen-induced liver injury in mice, the mechanism may be related to attenuating free radical injury and inhibiting lipid peroxidation and lowering release of inflammatory factors.

Objective:To identify gene mutations in a family with incontinentia pigmenti, in order to confirm pathogenic mutations.Methods:Clinical data were collected from all patients in a family with incontinentia pigmenti. DNA was extracted from peripheral blood samples obtained from the patients, healthy members in the family, and 100 unrelated healthy controls, and Sanger sequencing was performed for all exons and their flanking sequences of the NEMO gene.Results:Totally, there were 4 patients in the 4-generation family, who all presented with typical skin lesions and different symptoms. Genetic testing indicated that the proband and the other 3 patients all carried a heterozygous nonsense mutation c.1153C>T (p.Gln385X) at position 1153 in exon 8 of the NEMO gene, which led to the substitution of the glutamine codon (CAG) by the termination codon (TAG) at amino acid position 385. The mutation was not identified in the 14 healthy relatives or 100 unrelated healthy controls. The mutation cosegregated with incontinentia pigmenti in the family. Database searching confirmed the mutation to be a novel nonsense mutation, and it was considered as a very strong pathogenic locus according to the American College of Medical Genetic and Genomics guidelines.Conclusion:The mutation c.1153C>T in the NEMO gene is associated with the occurrence of incontinentia pigmenti in this family.

Beijing has been one of the epicenters attacked most severely by the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) since the first patient was diagnosed in one of the city's hospitals. We now report complete genome sequences of the BJ Group, including four isolates (Isolates BJ01, BJ02, BJ03, and BJ04) of the SARS-CoV. It is remarkable that all members of the BJ Group share a common haplotype, consisting of seven loci that differentiate the group from other isolates published to date. Among 42 substitutions uniquely identified from the BJ group, 32 are non-synonymous changes at the amino acid level. Rooted phylogenetic trees, proposed on the basis of haplotypes and other sequence variations of SARS-CoV isolates from Canada, USA, Singapore, and China, gave rise to different paradigms but positioned the BJ Group, together with the newly discovered GD01 (GD-Ins29) in the same clade, followed by the H-U Group (from Hong Kong to USA) and the H-T Group (from Hong Kong to Toronto), leaving the SP Group (Singapore) more distant. This result appears to suggest a possible transmission path from Guangdong to Beijing/Hong Kong, then to other countries and regions.
Genome, Viral ; Haplotypes ; Humans ; Mutation ; Open Reading Frames ; Phylogeny ; SARS Virus ; genetics