Objective: To explore a treatment method for vascular dementia. Methods: Sixty patients were randomly allocated into two groups. The observation group was treated with long-term needle-retaining method of scalp acupuncture and the control group was treated by medications. Evaluation was made respectively before and after the treatment by means of Hasegawa dementia scale (HDS), neurological function deficit (NFD) score and main symptom (MS) score. Results: There were statistically significant differences in HDS, NFD score and MS score in both groups (PO.01) before and after the treatment. There were statistically significant differences in HDS, NFD score and MS score between the two groups (P<0.05) before and after the treatment. The total effective rate was 86.8% in the observation group and 80.0% in the control group. There was a statistically significant difference in the effects between the two groups (P<0.05). Conclusion: This long-term needle-retaining method of scalp acupuncture is precisely effective for vascular dementia.

[Objective]To explore the effect of combined acupuncture and medicine on learning--memory abilities,and free radical system of hippocampus in vascular dementia(VD)rats.[Methods]The 48 Sprague Dawley rats were randomly divided into control(1),VD model(2),VD plus removed olfactory bulb model(3)and XIU Three Needles(4),Eugenol(5),combined acupuncture and medicine(6)groups.Morris maze tests were conducted for valuating the learning and memory abilities.The contents of MDA,SOD and GSH-Px activity of hippocampus were detected.[Results]In comparison with group 1,the average escape latency and travel distance prolonged significantly in group 2.In comparison with group 2,it shortened remarkably in groups 4 and 5.No significant differences were found between groups 1 and 2.In comparison with group 6,it prolonged in groups 4 and 5.No significant differences were found between them.The MDA content of hippocampus in VD model group was higher than that of control group(P

Objective To summarize the nursing experience for patients with filtering bleb infection after glaucoma filtering surgery. Methods We introduced the following nursing interventions such as accurate and prompt use of atropine eyedrop according to medical orders, local and intravenous administration of antibiotics and glucocorticoid, reasonable arrangement of medication, close monitoring of patients condition and reaction to treatment. In the same time we also supplied psychological nursing and health education. Results After intervention the symptoms disappeared and inflammation was under control, the filtering bleb became clear and anterior chamber exudation was absorbed, the visual acuity improved in 7 patients with 1 exception. The infection diffused and developed to endophthalmitis in this patient resulting in excavation of eyeball because of delaying hospital admission. Conclusion The keys to prevent filtering bleb infection were accurate, prompt and reasonable arrangement of medication according to medical orders and just-in-time adoption of nursing measures.

Objective To check the change of content of serum beta-apoliprotien(?-AP) and insulin(INS) in the patient with Alzheimer disease, and to reflect the effect of using different drug. Methods 60 cases of this disease and 30 cases of the control group were been detected by radioimmunoassay(RIA). Results The content of ?-AP in Alzheimer disease(AD) was obviously higher than that in control groups(P

Objective To explore the perioperative nursing care of cooled radiofrequency for refractory craniofacial postherpetic neuralgia.Methods 13 refractory craniofacial postherpetic neuralgia patients were reviewed. Results Visual analogue score significantly decreasedafter the treatment (P<0.01), and severe dropsy after treatment was relieved in 2~3 weeks. Conclusion The cooled radiofrequency iseffective on refractory craniofacial postherpetic neuralgia, while the perioperative nursing care was safe, feasible, and worthy for more application.

Objective To investigate the therapeutic efficacy and side effect of regional blockade using diprospan combined with oxycodone and acetaminophen taking orally which called balanced analgesia on severe tension-type headache. Methods 32 patients diagnosed as severe tension-type headache were treated with regional blockade using diprospan mixed with 0.4% lidocaine, combined with oxycodone and acetaminophen. The severity of pain (using numeric rating scales, NRS) and the duration of headache were observed and the side effects were recorded. Results There was a significant decrease in the severity of pain and the duration of headache after treatment compared with those before (P<0.01). No serious side effects were observed. Conclusion The balanced analgesia therapy mainly including regional blockade and oxycodone and acetaminophen can be significantly effective on the severe tension-type headache.

Objective:To establish an HPLC method for the determination of optical isomers in carfilzomib .Methods:The sample was separated on a Chiralpak OX-H column.The mobile phase consisted of n-hexane∶isopropanol∶alcohol (89 ∶5 ∶6, v/v/v) with a flow rat of 1.0 ml· min-1 .The detection wavelength was 220 nm.Results:The linear rang of enantiomer , diastereomer Ⅰand di-astereomer Ⅱwas 0.54-2.14 μg· ml -1,0.11-1.80 μg· ml-1 and 0.11-1.81 μg· ml-1(r≥0.998), respectively.The lower limit of quantification was 0.07-0.27 μg· ml-1 .The recoveries of optical isomers were within the range of 99.6%-100.9% with RSD of 1.13%-1.59%(n=9).Conclusion:The method is sensitive, simple, fast, accurate and specific, and suitable for the study of opti-cal isomers in carfilzomib .

Aim To study the protective effect and mechanism of geniposide on human umbilical vein endothelial cell(HUVEC) injury induced by H2O2.Methods The injured model was established by HUVEC treated with H2O2.HUVECs were cultured in vitro and divided into five groups:control group,H2O2 group and geniposide with different concentrations plus H2O2 group respectively.HUVECs were incubated with 400 ?mol?L-1 H2O2 for 12 hours in the absence or presence of various concentrations of geniposide pre-incubation.Survival rate of HUVECs was determined by tetrazolium assay.The intracellular activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),total nitric oxide synthase(NOS) and extracellular nitric oxide(NO) level were detected.The intracellular reactive oxygen species(ROS) level and the apoptotic index and cell cycle alteration were detected by flow cytometry.Results Geniposide concentration-dependently increased the viability of injured endotheial cells,while increased the activity of SOD,GSH-Px,NOS and NO production.The intracellular ROS level and the apoptotic index were reduced by geniposide.The cell proliferation was increased with geniposide incubation.Conclusion Geniposid may be a potential anti-oxidation agent which has a protective effect against HUVEC injuries induced by H2O2.

Objective: To establish a determination method for the enantiomer in safinamide mesilate. Methods: A Chiralpak ASH (250 mm ×4. 6 mm, 5 μm) column was used with the mobile phase of n-hexane-ethanol-diethylamine (75 ∶ 25 ∶ 0. 1). The flow rate was 1. 0 ml·min-1 . The wavelength was set at 225 nm. The column temperature was 35℃. Results: The resolution of safinamide mesilate and the enantiomer was above 2. 0. The linear range of them was 1. 007-2 517. 500 μg·ml-1 and 0. 909-2273. 200 μg·ml-1 ,respectively(r = 0. 999 0). The average recovery of the enantionmer was 104. 9% with RSD of 2. 3% (n = 9). Conclusion: The method is accurate and rapid, and suitable for the determination of the enantiomer in safinamide mesilate.

Objective:To establish an HPLC method to determine the content of carfilzomib for injection. Methods:The chroma-tographic column was a Waters symmetry C18(250 mm ×4.6 mm, 5 μm)column, the sample solvent was methanol, the mobile phase was 0. 05% trifluoroacetic acid-acetonitrile(57 ∶43) at a flow rate of 1. 0 ml · min-1 , the detection wavelength was 210 nm, the col-umn temperature was 25℃ and the injection volume was 10 ml. Results:The linear range of carfilzomib was 120-600 μg·ml-1 ( r=0. 999 7). The average recovery was 100. 4%( RSD=0. 24%,n=9). Conclusion:The method is rapid and effective, which can be used for the content determination of carfilzomib for injection.