Objective To contrast the message of the state of postgraduate patient communication before or after evaluation and put forward the solution.Methods Each 200 questionnaires were randomly sampled that of patients who were hospitalized in the department of ENT department of the seconed affiliated hospital of Chongqing Medical University.Results The ratio of satisfaction was 56.43%;34.1%patients considered the communication was common,9.49%patients considered the communication was dissatisfying before evaluation,but the ratio was 78.8%,14%,7.5%respectively after evaluation.Before the evaluation,65.4%patient considered the service attitude affecting the communication,48%patient considered the behavior of the doctor affecting communication,52.5%patient considered the speech of the doctor,while after evaluation,the ratio was 12%、14.5%、23.5%respectively.Conclusion Evaluation improved the doctor patient communication,but postgraduates were required to improve speech and behavior arts.

OBJECTIVE:To study the effect of ginsenoside Rg1 on facial nerve regeneration in ovariectomized rats.METHODS:48 ovariectomized SD rats were randomly assigned into sham operation group (normal saline),model group (normal saline),nerve growth factor group (NGF,10 mg?kg-1) and ginsenoside group (10 mg?kg-1).Ovariectomized model were induced and received relevant drugs.The behavior changes of rats were observed.The morphology changes of facial nucleus were detected under transmission electron microscope (TEM) and light microscopy.BDNF expression of facial nucleus was determined with immunohistochemistry.RESULTS:The morphology of the facial nucleus was improved.The BDNF expression of 3D facial nucleus in model group,NGF group and ginsenoside group were increased after facial nerve was injured.The BDNF expression was decreased on 7th day while increased on 14th day.The thickness of regenerating nerve fiber in ginsenoside group was superior to in model group (P

Objective To study the diagnosis and treatment of adrenal tumors. Methods 1 006 cases of adrenal tumors treated from 1957 to 2000 were reviewed. Results Of the 1 006 cases of adrenal tumors,845(84.0%) were functional with 12(1.4%) malignant and 161(16%) nonfunctional with 11( 6.8%) malignant.There were 180 incidentaloma including 19 pheochromocytoma.A total of 996 tumors have been surgically removed and 10 cases underwent surgical exploration only.The total mortality rate was 0.3%. Conclusions Adrenal tumors occurred mostly in patients of 30～50 years of age.The prevalence of Cushing syndrome was more in females than in males.Ultrasonography and CT are the method of choice for the localization of the tumor whereas hormone levels should be determined both in the symtomatic and asymtomatic patients with adrenal tumors.Functional adrenal tumors and solid incidentaloma of large size should be surgically removed while asymtomatic incidentaloma less than 3 cm in size could be observed and followed up and surgery might be undertaken when indicated.

Cell therapy refers to a method that transplants stem cells or differentiated functioning cells into diseased regions to compensate the losing functions of diseased cells or uses the stem cells for treatment of diseases after in vitro genetic manipulation. After more than 30 years of study, great progress in hepatocyte transplantation technique has been acquired. This technique can acquire in situ liver transplantation effects. Hepatocyte transplantation, as an effective method for treatment of fulminant hepatic failure, has been widely confirmed in the animal model experiments and clinical practice. In addition, application of remedial hepatic stem cells can solve the problems regarding the source, number and immune rejection of hepatocytes, and offers a wide prospect for treatment of acute and chronic liver diseases. There are many problems about the study on hepatic stem cells. Up to date, many manuscripts addressing animal hepatic stem cell transplantation have been found, but few of them focus on the case report of treatment of liver disease by hepatocyte transplantation. Therefore, study on hepatic stem cells is still in its initial stage. The study methods should be developed and improved.

The efficacy of acupuncture for pain diseases is significant, however, the unified understanding re garding mechanisms of acupuncture analgesia has not been fully reached. In this paper, it is believed that uncoordi nated muscle movement is one of the important causes for pain, while acupuncture could enhance the contact of nerve cells and improve the synergy of muscle movement, so as to achieve the aim of pain relief and even cure.
Acupuncture Analgesia ; Humans ; Muscle Contraction ; Muscles ; physiopathology ; Pain Management

Objective To ascertain the optimum condition for extracting Rhizoma polygoni cuspidati and Radix angelicae sinensis with alcohol. Method With extract yield and the amount of polydatin as index,the primary factors of affecting the extraction were optimized. Result The optimum extraction condition was as follows:taking 60% alcohol as solvent,the meterials were refluxed and extracted two times with 10 and 8 times volume of alcohol for 1.5 h and 1.0 h,respectively. Conclusion The optimum extraction process provides an experimental basis for industrial production.

Objective To elevate ability of diagnosing adrenal tumors. Methods B-ultrasonography were performed on 385 cases;CT scanning were on 385 cases;IVU were on 380 cases;MRI were on 180 cases;Doppler were on 63 cases;DSA were on 16 cases;MRA were on 28 cases;MIBG were on 34 cases;3 cases were performed tumors biopsy directed through CT. Results The accurate localization rate of APA,Cushing tumors,Pheo,myelolipoma with B-ultrasonography were 92.7%,88.3%,100.0%,100.0% respectively.The accurate qualitative rate of diagnosis were 83.2%,86.7%,83.4%,100.0% respectively.With CT scanning were 98.2%,98.7%,100.0%,100.0% and 90.5%,92.3%,83.4%,100.0% respectively.With MRI were 90.4%,96.5%,100.0%,100.0% and 72.8%?93.5%?85.8%?100.0% respectively. Conclusions The overall data of imaging is helpful in diagnosis of adrenal tumors.Correct localization of the tumors is the key step for the treatment.

Objective To evaluate the comprehensive treatment results of cystitis glandularis. Methods A total of 65 cases of cystitis glandularis underwent cystoscopy.The results showed follicular or villous changes in 39 cases,papillomatous in 15,chronic inflammation in 8 and no obvious change in 3.Transurethral electric resection or partial cystectomy were performed.After surgery all the cases received mitomycin-c bladder irrigation at high dose (40 mg once) regularly for 2 years.During the follow-up cystoscopy was periodically performed and the biopsy samples were pathologically examined with all the cases. Results The mean follow-up was 29 months (range,7 to 72 months).Of the 65 cases,60 fully recovered with transitional cell surface covering the bladder musoca gradually;4 had relapse;and 1 developed canceration (adenocarcinoma). Conclusions Transurethral resection of bladder tumors or galvanocautery is the essential therapy for cystitis glandularis.After surgery the bladder irrigation with mitomycin-c at high dose can be helpful for urothelium recover in histology.

Objective To detect serum level of anti-M-type phospholipase A2 receptor (PLA2R) antibody in elderly patients with idiopathic membranous nephropathy (IMN) and to explore its clinical significance in IMN disease.Methods A total of 134 patients with renal biopsy-proved glomerular diseases were enrolled in this study,including 42 elderly cases with IMN,45 non-elderly cases with IMN,19 elderly cases with minimal change nephropathy (MCN),12 elderly cases with IgA nephropathy (IgAN),8 elderly cases with hepatitis B-associated membranous nephropathy (HBV-MN) and 8 elderly cases with focal segmental glomerulosclerosis (FSGS).Western blotting was used to detect serum anti-PLA2R antibody and the correlation of anti-PLA2R antibody with laboratory parameters in elderly IMN patients was analyzed.Results The elderly and non-elderly patients with IMN showed that the positive rate of anti-PLA2R antibody was 71.4％ and 73.3％,respectively (P＞ 0.05).The positive rate of anti-PLA2R antibody in elderly IgA nephropathy,HBV-MN and MCN patients was 16.7 ％,12.5％ and 5.3％ respectively.Anti-PLA2R antibody was not detected in serum from elderly FSGS patients.The positive rates of serum anti-PLA2R antibody were significantly higher in both elderly and non-elderly IMN patients than in elderly patients with secondary MN and other types of glomerlonephritis (P ＜ 0.01).Furthermore,anti-PLA2R autoantibody level was positively correlated with 24-hour urine protein level and negatively correlated with the concentration of serum albumin in elderly patients with IMN (P＜0.01).Conclusions Anti-PLA2R antibody is a sensitive serological marker of IMN.Detection for anti-PLA2R antibody might be helpful for the diagnosis of both elderly and non-elderly IMN and for the monitor of IMN disease severity.

Pancreatic tuberculosis (PTB) is a rare,chronic,specific and infectious disease which is generally secondary to tuberculosis at the common sites of pancreas,and it has a high misdiagnosis rate due to the hidden onset and nonspecific symptoms of PTB.A patient with PTB was admitted to the First Affiliated Hospital of Zhengzhou University in June 2014.Before operation,the space-occupying lesions of the head of pancreas were detected by preoperative imaging examination,and the patient was regarded as with pancreatic cancer.Intraoperative exploration showed cystic duct involvement,and the granulomatous inflammation was detected by rapid pathological examination using frozen section technique,after that the patient received granuloma resection+cholecystectomy according to suspected PTB.The diagnosis of PTB was confirmed by postoperative pathological examination,and the patient received liver-protective and anti-tuberculosis treatments after discharge.