Objective:To review the X-ray manifestations of the skeletal lesions in children with Langerhans cell histocytosis(LCH),and to evaluate the X-ray examination in the diagnosis of LCH.Methods:The skeletal plain films of 35 children with LCH were analysed retrospectively.AⅡ cases were confirmed by pathology.Results:Among 35 cases,skull destructions were found in 24(68.6%),vertebral destruction in 8(22.9%),long bone destruction in 12(34.3%),flat bone and irregular bone destruction in 4(11.4%).Eosinophilic granulomas mainly involved skull,vertefra and long bones,while Letterer-Siwes disease,Hand-Schuller-Christian disease and intermediary type mainly involved skull bones.Conclusion:Multilocation,diversity and changeability of the skeletal lesions are the x-ray features of LCH in children.X-ray examination and follow-up play an important role in the diagnosis of LCH.

Objective To analyze the clinical efficacy of thalidomide combined with anti tuberculosis drugs in the treatment of pulmonary tuberculosis,in order to evaluate its application value and safety. Methods Seventy-two pulmonary tuberculosis patients in the Qianshan Hospital of Anshan from Jan. 2012 to Aug. 2014 were selected as the subject. Patients were randomly divided into control group and observation group with 36 cases in each group. Patients in the control group were only received anti-tuberculosis drug treatment,while in the treatment group were given thalidomide combined with anti tuberculosis drug treatment. Efficacy rate of thalidomide treatment after two weeks was evaluated by X-ray examination. Incidence of sputum smear negative conversion rate and adverse reaction were recorded. Results There were 10 cases with obvious tuberculosis absorption,19 cases with lesions absorption,7 cases with no absorption in observation group. In control group, there were 5 cases with obvious tuberculosis absorption,12 cases with lesions absorption,17 cases with no absorption and the lesion increased in 2 cases. The focus absorption in observation group was superior to the control group( Z =11. 854,P ﹤0. 001 ). There were 6 cases with void closure,4 cases with decreased void closure,1 cases with unchanged and 1 cases with new cavity in observation group. There were 1 cases with void closure,3 cases with decreased void closure,6 cases with unchanged and 3 cases with new cavity in control group,and there was significant difference(Z=15. 536,P﹤0. 001). Sputum smear negative rate in observation group after treatment was 85. 0%(17/20),significantly higher than that in control group(50. 0%(11/22),χ2=5. 775,P=0. 016). Cases of adverse reactions in observation group was 8(22. 2%),lower than that in control group(17 cases,(47. 2%);χ2 =4. 963,P=0. 026). There were 2 cases with abnormal liver function without changing the original treatment symptomatic liver after treatment,in observation group. Of 8 cases with abnormal liver function in control group,5 cases change treatment schedule including 3 cases of modified pyrazinamide for streptomycin,2 cases converted to rifampicin for Levofloxacin. The syndromes including eukopenia,skin rash, anorexia and lethargy were disappeared after treatment. Conclusion The effect of Thalidomide combined with anti tuberculosis drugs in the treatment of pulmonary tuberculosis is significant and safety,which can be used as a routine drug treatment of tuberculosis in clinical trials.

Objective To observe the clinical effect of subcutaneous drainage tube in the prevention of abdominal surgical incision infection.Methods According the admission sequence,290 abdominal surgery patients were selected and randomly divided into the two groups,each group 145 cases.The patients of the observation gronp were placed subcutaneous drainage tube after operation,and the patients of the control group were not placed subcutaneous drainage tube.The wound healing,wound infection rate,and secondary debridement incidence of the two groups were observed and compared.Results The successful surgery were in 190 patients,and there was no patient in death.The incision healing rate of first grade in the observation group was 97.93％,which in the control group was 89.66％,The incision infection was in 3 cases (2.07％),which in the control group was 12 cases (8.28％).There were all statistically significant differences between the two group (all P ＜ 0.05).The secondary debridement of the observation group was in one case,which in the control group was in 7 cases,and the secondary debridement incidence of the observation group was significantly lower than that of the control group(P ＜ 0.05).Conclusion Placing the subcutaneous drainage tube for abdominal surgery patients can reduce the occurrence of wound infection and secondary debridement,and beconducive to postoperative recovery.

[Objective] The custom in Huangdi Neijing has been discussed in order to inspire the health prevention for modern people. [Method] Using traditional philological research methods, through studying the relevant literature carefully, having combined with the related historical research achievements of the life in Qin and Han Dynasties, the influence of customs on people's way of living in Huangdi Neijing has been summarized. [Results] Huangdi Neijing has been as a medical classic, having recorded many contents including the ancient philosophy, science and technology etc. Many of them have reflected the contents of the social customs at that time, concluding the diet, beliefs, rituals and etiquette. And it refers to some historical records at the time in order to pry into the custom during the period of Huangdi Neijing. [Conclusion]The research of customs in Huangdi Neijing has discussed to help avoid the bad customs to restore the normal living habits in order to achieve the purpose of health and disease prevention.

Ozostomia is presently a common disease or symptom. By differentiation, TCM classifies it into types of accumulation of stomach heat, blazing spleen and stomach, phlegm heat accumulated in lung, blazing heart and spleen, blood stasis inside, retention of food in the stomach, too much fire of kidney deficiency and overstrain injuring spleen. It gets good effect in treating ozostomia by differentiation of signs and symptoms.

Objective To investigate the value of Pelvic Floor ultrasound in diagnosis of female Stress urinary incontinence (SUI).Methods Pelvic floor ultrasound were performed in a total of 200 cases of female.Among 55 patients with SUI by clinical were selected as the study group,145 without SUI as the control group.Bladder neck descent (BND),posterior urethrovesical angle (PUVA) in Valsalva state,urethral rotation angle (URA) and levator hiatus area (LHA) in Valsalva state were measured to assess the value in diagnosing SUI.Results There were significant difference in BND、PUVA&LHA in Valsalva state,URA between two groups (all P ＜ 0.01).AUC of 4 data combined is 0.98.Conclusion Measuring BND,PUVA&LHA in Valsalva state,URA by pelvic floor ultrasound have high diagnostic value in female SUI.

This paper introduces the perioperative nursing of 20 cases of branchial cysts in newborn with cyst extraction. The nursing care focused on body position,respiratory care,diet management and wound care,which alleviated the patients' dyspnea,prevented respiratory aspiration and promoted wound healing. All the 20 newborn recovered well without recurrence in the four years after the operation.

Adenovirus vectors are promising delivery systems for gene therapy. We established a new process for clinic trial of recombinant adenovirus vectors using a novel disposable bioreactor. The suspension HEK293 cells were inoculated into a 5 L disposable bioreactor with parameters control of pH, DO, agitation and temperature. After 6 days of a fed-batch culture, the final cell density reached 2.0 x 10(6) cells/mL. The culture was infected with Ad-IFNγ at an MOI of 30. The harvest was performed at approximately 48 h post-infection and crude viral lysate was obtained after 3 freeze/thaw cycles and centrifugation. The maximum titers of crude viral lysate was 1.49 x 10(13) Infectious units (IFU) and the bulk product specific was 3,800 IFU/cell. Purified Ad-IFNγ by anion-exchange chromatography and the final recovery of infectious unit reached 35.9%. The result demonstrates that an efficient and stable process of producing Ad-IFNγ using a disposable fed-batch bioreactor is established.
Adenoviridae ; growth & development ; Batch Cell Culture Techniques ; Bioreactors ; Cell Count ; Genetic Therapy ; Genetic Vectors ; HEK293 Cells ; Humans ; Virus Cultivation ; methods

Objective To detect serum levels of 25 ? hydroxyvitamin D (25 [OH] D) and matrix metalloproteinase 9(MMP?9)in patients with chronic spontaneous urticaria(CSU), and to explore their significance. Methods Totally, 56 patients with CSU and 25 healthy controls were enrolled into this study. High?performance liquid chromatography tandem?mass spectrometry(LC?MS/MS)and enzyme?linked immunosorbent assay(ELISA) were performed to measure serum levels of 25(OH)D and MMP?9 respectively in these subjects. Statistical analysis was carried out by two independent samples t?test or Wilcoxon rank sum test for comparisons of the above indexes between the two groups, and by Spearman rank correlation analysis for assessment of relationships between the serum levels of 25(OH)D and MMP?9 as well as between the two indexes and disease severity. Results Of the 56 patients, 19 were diagnosed with mild CSU, 26 with moderate CSU and 11 with severe CSU. Compared with the control group, the patient group showed significantly lower serum levels of 25(OH)D(21.74 ± 6.04 vs. 30.17 ± 2.21μg/L, P<0.05), but significantly higher serum levels of MMP?9(Median[P25-P75]:291.55[166.18-594.46]vs. 138.46[94.27-233.12]μg/L, P<0.05). In the patient group, the serum level of 25(OH)D was negatively correlated with that of MMP?9 (rs=-0.26, P<0.05), but uncorrelated with the severity of CSU(rs=-0.20, P>0.05);the serum level of MMP?9 was positively correlated with the severity of CSU(rs=0.27, P<0.05). Conclusion Vitamin D and MMP?9 may play a role in the occurrence of CSU.

Objective To investigate the clinical features and occurrence regularity of drug induced liver injury,so as to provide reference for clinical medication.Methods The choice of hospital 2012 -2014 reported adverse drug reaction monitoring center data,including DILI 30 cases,DILI related data to classify statistics,compre-hensive analysis.Results DILI was of many influence factors,the time of occurrence of large differences,partial onset occult,and related to a drug to immune enhancement agent most(23.33%),followed by anti -tumor drugs (20.00%)and lipid regulating agent(16.67%).Conclusion The medical staff should enhance the understanding of DILI medicine source disease,strengthen the monitoring of adverse drug reactions,as far as possible to reduce the occurrence of DILI,and make its harm to a minimum,reduce the patients pain and medical expenses of disease.