Objective To understand the prevalence and awareness of Toxoplasma gondii of the pregnant women in Bazhou City,Hebei Province,so as to provide the evidence for formulating effective prevention and control interventions. Methods The pregnant women with prenatal screening in the Fourth People’s Hospital in Langfang City were selected and investigated with questionnaires. A part of the pregnant women were randomly sampled and received the ELISA test of the antibodies against T. gondii. Results Totally 672 pregnant women accepted the questionnaire survey,with an awareness rate of 19.49%(131/672). Totally 526 pregnant women of them were randomly selected to take the ELISA test,with a positive rate of 13.88%(73/526). The awareness rate and infection rate of T. gondii were related to the education level,occupation and place of residence (c2=5.89-17.62,all P<0.05),and the infection rate was negatively related to the awareness rate(c2=5.37,P<0.05). Con?clusion Increasing the awareness rate of toxoplasmosis knowledge is one of the effective methods to control the disease. There?fore,the health education about T. gondii should be carried out actively.

This study aimed at investigating the effects and mechanisms of 7-O-succinyl macrolactin A in inhibi-ting human lung cancer. After treatment of human lung cancer cell lines H460 with 7-O-succinyl macrolactin A, MTS assay was employed to determine cell proliferation;crystal violet staining was used to detect cell adhesion of H460;transwell chamber assay and wound healing assay were performed to evaluate cell invasion and migration;and flow cytometry assay was adopted to evaluate cell cycle. Western blotting and real-time PCR were also employed to determine the expression of β-catenin, c-Myc, Cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2 , Survivin and MMP-2/9. The phosphorylation of AKT and mTOR was determined as well. In vitro prolifera-tion of H460 was inhibited significantly by 7-O-succinyl macrolactin A. Cell adhesion, invasion and migration abilities were also attenuated. Western blot and real-time PCR showed that the expressions of β-catenin, c-Myc, cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9 were down-regulated by 7-O-succinyl macrolactin A. It was also found that phosphorylation of AKT and mTOR was inhibited by 7-O-succinyl macrolactin A. 7-O-succinyl macrolactin A can inhibit the in vitro growth and invasion of human lung cancer cell lines H460.

Objective To investigate the expressions and clinical significance of PPAR? and MMP-9 in the genesis and development of hypertensive disorder-complicating pregnancy(HDCP).Methods The expressions of PPAR? and MMP-9 in placenta of 31 normal pregnant women(control group) and 36 pregnant women with hypertensive disorder-complicating pregnancy(experimental group) were detected by using SP method of immunohistochemistry,and the relationship between PPAR? and MMP-9 expressions and hypertensive disorder-complicating pregnancy was explored.Results ① PPAR? expression in placental tissues was significantly higher in the experimental group than in the control group(P

Objective:To establish an HPLC method for the determination of isomeric impurity in fasudil hydrochloride. Meth-ods:The chromatographic method was carried out on a Kromasil 100-5 Phenyl C18 column with phenyl bonded silica as the filler (250 mm × 4. 6 mm, 5 μm), and phosphate buffer (10 mmol· L-1 ammonium dihydrogen phosphate, adjusting pH to 4. 0 with 1% phos-phoric acid) -acetonitrile (80∶ 20) was used as the mobile phase. The detection wavelength was 275nm and the column temperature was 40℃. The flow rate was 1. 0 ml· min-1 , and the injection volume was 10 μl. Results:Fasudil hydrochloride and its derivative was linear within the range of 0. 148-2. 960 μg·ml-1(r=1. 0000) and 0. 101-2. 014μg·ml-1(r=0. 999 9), respectively. The av-erage recovery of isomer impurity in fasudil hydrochloride was 101. 9% with RSD of 0. 98%(n=9). Conclusion:The method is sim-ple,accurate and reproducible,which can be used for the quality control of fasudil hydrochloride and its isomer.

BACKGROUND: Olfactory ensheathing cell (OEC) transplantation can promote the recovery of neurological function in rats with cerebral infarction, while the migratory pattern of transplanted OECs and the relationship between OECs migrated to various encephalic regions and plasticity upregulation and recovery of neurological function of the encephalic region are still unknown.OBJECTIVE: To observe the migratory pattern and therapeutic value of OEC transplantation in rats with cortical cerebral infarction.DESIGN, TIME AND SETTING: Randomized, controlled, cell transplantation observation experiment was conducted between June 2002 and January 2004 at the Laboratory of Department of Neurology, the First Affiliated Hospital of Sun Yet-San University and Animal Experimental Center of SUN YET-SEN University, Guangzhou, Guangdong Province, China.MATERIALS: Sprague-Dowley rats, 2.5-month-old, were used for olfactory ensheathing cell culture. 150 Sprague-Dowley rats,60-90 days, were used to replicate stroke-prone reuovascular hypertensive rat model using two-kidney two-clip method.METHODS: We purified OECs with differential time adherent method and labeled OECs with Hoechst 33342 before transplantation. Seventy stroke-prone renovascular hypertensive rats were used to prepare middle cerebral artery occlusion model.model rats were randomly allocated to 3 groups to receive OEC transplantation: peri-mfarct cortex transplantation group,contralateral cortex transplantation group and bilateral transplantation group.MAIN OUTCOME MEASURES: The recovery of motor and sensory function was observed at 2 weeks and 6 weeks after transplantation with behavior and sensory function examination; the survival and distribution conditions of transplanted ceils were observed under the fluorescence microscope.RESULTS: The recovery condition of motor and sensory function of rats in bilateral transplantation group was obviously better than that of rats in peri-infarct cortex transplantation group and contralateral cortex transplantation group (P ＜ 0.01), nerve fiber number and positive signal value of growth associated protein-43 in the marginal zone of cerebral infarction were also more than that in peri-infarct cortex transplantation group and contralateral cortex transplantation group. Transplanted cells in peri-infarct cortex transplantation group migrated to infarct and contralateral cortex along corpus callosum, transplanted cells in contralateral cortex transplantation group migrated to midline and infarct cortex along corpus callosum, transplanted ceils in bilateral transplantation group migrated along corpus callosum and could be seen in bilateral cortices while more in infarct cortex.CONCLUSION: Transplanted OECs can survive for a long time period, and these cells not only confine to injection point but also can migrate to infarct and contralateral cortex along corpus caliosum to promote the recovery of neurological function of rats with cerebral infarction, the effect is more significant in bilateral transplantation.

Objective To observe the time-intensity curve characteristics of contrast agents in intraocular tumor.Methods A total of 236 patients (238 eyes) with intraocular tumor were enrolled in this study.All the patients received regular ophthalmologic examination,two dimensional ultrasound,color doppler ultrasonography and contrast-enhanced ultrasonography.There were 166 patients (166 eyes) with choroidal melanoma,16 patients (18 eyes) with choroidal metastatic carcinoma,52 patients (52 eyes) with choroidal hemangioma,two patients (two eyes) with retinal hemangioma.The whole process of contrast-enhanced ultrasound were recorded, and exported as t images of Dicom format.These images were processed by Sonoliver software (Tomteck Company, Germany) to drawn the time-intensity curve of contrast agents in the intraocular tumors.Results All intraocular lesions were completely filled with contrast agent,concentric filling from the periphery to the center can be documented in some cases.The time-intensity curve of choroidal hemangioma and retinal hemangioma were basically the same.The time-intensity curve of choroidal melanoma and choroidal metastatic carcinoma were also basically the same.In the filling phase,all tumors were rapid filling type.In the regression phase,contrast agent subsided earlier than in control tissue within the melanoma or metastatic carcinoma lesions,but subsided synchronous or slightly faster than in control tissue within the choroidal hemangioma and retinal hemangioma lesions.Among 166 eyes with choroidal melanoma,138 eyes (83.1％) were in full compliance with the above changes,28 eyes (16.9％) were largely in line with these changes.All the eyes (100.0％) with choroidal metastatic carcinoma,choroidal hemangioma and retinal hemangioma were in full compliance with the above changes.Conclusion Time-intensity curve is quickly filling and fast regression for malignant intraocular tumors,but is quickly filling and slow regression for benign intraocular tumors.

Objective To discuss the diagnosis characteristics and clinical value of posterior vitreous detachment (PVD) by color Doppler flow imaging(CDFI). Methods One thousand and two cases were examed by CDFI for the diagnosis standard and property of PVD. Results Typical acoustic properties of PVD: the B scan may show a very fluid thick line. There were two types complete PVD and part PVD based on the vitreous separated from the optic nerve. Kinetic echography typically showed a very fluid, undulating after movement of the completely PVD. Part PVD had more than one dot with attachments to the retina and less mobile than complete PVD. There was not blood flow signal on the PVD by CDFI. Conclusions Ultrasonography for PVD not only exactitudes,but also provides foundation for clinic.

Objective To analyze the application effect and safety of streamlined liner of the pharynx airway (SLIPA) anesthesia in painless fiberoptic bronchoscopy (FB).Methods Two hundred and eighty-two patients who undorwent FB examination in Kaifeng Second People's Hospital from January 2015 to January 2017 were selected and divided into observation group and control group,with 141 patients in each group.The patients in observation group were given SLIPA assisted anesthesia;the patients in control group were given endoscopic mask assisted anesthesia.The oxygen saturation (SpO2),systolic blood pressure (SBP),diastolic pressure (DBP) and heart rate (HR) of patients in the two groups were monitored.The changes of SBP,DBP and HR,the incidence of adverse reactions and the satisfaction of patient to the anesthesia were compared between the two groups.Results The fluctuation of HR,SBP and DBP of patients in the observation group was (3.52 ± 2.92) times · min-1,(7.04 ±5.30) mmHg(1 mmHg =0.133 kPa),(1.52 ± 1.63) mmHg respectively and in the control group was (5.52 ± 1.89) times · min-1,(30.12 ± 8.88) mmHg,(4.06 ± 5.78) mmHg respectively;the fluctuations of HR,SBP and DBP of patients in the observation group were significantly less than those in the control group(P ＜ 0.05).In the observation group,12 patients with cough,8 patients with abdominal distension,6 patients with pharyngeal pain,2 patients with SpO2 ≤90％,4 patients with SpO2 ＜ 85％;in the control group,57 patients with cough,32 patients with abdominal distension,47 patients with pharyngeal pain,28 patients with SpO2 ≤90％,19 patients with SpO2 ＜ 85％.The incidence of adverse reactions of patients in the observation group was significantly lower than that in the control group (x2 =27.10,61.82,41.59,72.46,87.75;P ＜0.05).The anesthesia satisfaction of patients in the observation group(97.87％) was significantly higher than that in the control group(82.98％) (x2 =29.97,P ＜ 0.05).Conclusion SLIPA assisted anesthesia has good clinical effect and safety in patients with painless FB.

Objective To discusse the expression and clinic opathologic significance of vascular endothelial growth factor(VEGF) and microvessel density(MVD) in primary liver cancer,an immunohistochemical staining.Methods The expressions of VEGF and Ⅷ factor were determined in 60 selected operation specimen from patients with primary liver carcinoma.Results It showed that the positive expression rate of VEGF in primary liver cancer was 63.3%,the MVD was (30.76?11.56).Among them,the MVD in positive VEGF expression was (46.37?15.85),and the MVD in negative expression was (18.97?8.68),there were great statistical difference between them(P0.05).Conclusion This study indicates that VEGF can facilitate the formation of microvessel of the tumor and has correlation with the growth,infiltration and metastasis of primary liver cancer.

Objective To compare the accuracy of intraocular lens(IOL)power calculations by using 6 formulas(SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ)in different axial lengths of the eye,in order to improve the accuracy of predicating IOL powers for patients after cataract surgery.Design Retrospective comparative clinical analysis.Participants 169 patients(169 eyes)with age-related cataract underwent regular phacoemulsification and posterior chamber IOL implantation.Methods 169 eyes with cataract were divided into five groups according to the axial lengths:AXL≤22mm,22mm28.4mm.The axial lengths and keratometry of the 169 eyes were measured with A-mode ultrasound before operation,and the actual postoperative refraction was measured with the methods of computer and retinoscopy optometry when patients could gain best corrected visual acuity one month after surgery or later.Inputting the axial lengths,keratometry and IOL powers into A-mode ultrasound,the predicted postoperative refraction was calculated with SRK-T,Haigis,Binkhorst-Ⅱ,Hoffer Q,Holladay-1,SRK-Ⅱ formulas,and the differences of predicted and actual postoperative refractions in different axial lengths were compared.Main Outcome Measures The absolute value with the differences of the predicted and actual postoperative refractions,i.e.absolute predicted errors.Results(1)Comparing the difference of predicted and actual postoperative refraction,there was no statistical significance between the five formulas in the groups of axial length(AXL)≤22mm,22mm28.4mm,but the average absolute predicted error of Haigis and SRK-T formulas is significantly smaller than SRK-Ⅱand Binkhorst-Ⅱ formulas.Conclusions In existing sample size,there was no obvious influence on postoperative refraction when using other five formulas except Binkhorst-Ⅱ.But in the extremely long AXL group,Haigis and SRK-T formulas have some ascendancy over the other formulas.