Objective To investigate the level of plasma malondialdehyde (MDA)and coenzyme Q10 (CoQ10)in patients with type 2 diabetes mellitus,diabetic leg ulcer(DLU).Methods The DLU group consisted of 15 patients with leg ulcers and type 2 diabetes mellitus (7 men and 8 women).The diabetes group consisted of 15 patients with type 2 diabetes mellitus (8 men and 7 women).Ten healthy individuals,age and sex matched with the patients,were used as a control group.Laboratory investigations in the form of glycosylated hemoglobin,plasma MDA and plasma CoQ10 were assessed for all patients and controls.Results The study revealed that in the diabetes group,especially in the DLU group,the MDA level [(7.07 ±3.15)μmol/L and (3.15 ±2.66)μmol/L]was signifi-cantly higher than control group [(4.28 ±0.41)μmol/L]and the index difference between them (F =104.675,P <0.01),while CoQ10 levels in the two groups data[(0.33 ±0.16)mg and (0.16 ±0.16)mg ]were significantly lower than the control group [(0.86 ±0.13)mg](F =46.709,P <0.05).Conclusion Type 2 diabetic patients, especially with DLU,are at increased risk of oxidative stress manifested by increased plasma MDA.

This study aimed at investigating the effects and mechanisms of 7-O-succinyl macrolactin A in inhibi-ting human lung cancer. After treatment of human lung cancer cell lines H460 with 7-O-succinyl macrolactin A, MTS assay was employed to determine cell proliferation;crystal violet staining was used to detect cell adhesion of H460;transwell chamber assay and wound healing assay were performed to evaluate cell invasion and migration;and flow cytometry assay was adopted to evaluate cell cycle. Western blotting and real-time PCR were also employed to determine the expression of β-catenin, c-Myc, Cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2 , Survivin and MMP-2/9. The phosphorylation of AKT and mTOR was determined as well. In vitro prolifera-tion of H460 was inhibited significantly by 7-O-succinyl macrolactin A. Cell adhesion, invasion and migration abilities were also attenuated. Western blot and real-time PCR showed that the expressions of β-catenin, c-Myc, cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9 were down-regulated by 7-O-succinyl macrolactin A. It was also found that phosphorylation of AKT and mTOR was inhibited by 7-O-succinyl macrolactin A. 7-O-succinyl macrolactin A can inhibit the in vitro growth and invasion of human lung cancer cell lines H460.

Objective To investigate the relationship between gallbladder stone with blood lipid levels ,body weight index (BMI) and distribution of TCM constitution .Methods A total of 698 cases of gallstone in our two hospital from September 2013 to February 2015 were selected as the experiment group ,and contemporaneous 253 cases of abdominal hernia ,gallbladder polyp and other diseases were included into the control group .The TCM constitution identification investigation was performed and the TCM constitution distribution was observed .The blood lipid levels and BMI were compared between the two groups .The risk of blood lipid level and BMI to gallstone occurrence was evaluated .Results The normal constitution in the experimental group accounted for 12 .2% ,the top three places of the biased constitution were phlegm‐damp nature ,Qi deficiency nature and damp heat nature ,which accounted for 29 .4% ,17 .0% and 11 .0% respectively .The TC and VLDL‐C level and BMI in the male and female patients of the experiment group were higher than those in the control group ,moreover the TG level in the female patients of the experiment group was significantly higher than that in the control group ,the difference was statistically significant (P< 0 .05) .The increase of TC (OR=2 .87) ,VLDL‐C(OR=6 .32) and BMI (male:OR=2 .43 ,female:OR=2 .01) were the risk factors of gallstone occurrence . Conclusion Increase of TC ,VLDL‐C and BMI are the risk factors of gallstone occurrence ,the TCM constitution of gallstone pa‐tients is dominated by the phlegm‐damp nature .

Objective To determine the relation between enlarged perihepatic lymph node (PLN) and viraemia,and to find out whether there is a difference in PLN size between the healthy individuals and patients with hepatitis C virus (HCV) infection. Methods Seventy-four patients with HCV infection and 283 healthy individuals were examined by two-dimensional ultrasound. The length and thickness of lymph node were measured. The lymph-node area index(LN area index) was calculated. The LN area index was evaluated by the ROC curve and compared with serum HCV core antigen (HCV-Ag) levels by correlation analysis. Results Detection rate, the length and thickness, LN area index of group with HCV infection were significantly different by compared with the normal control group ( P < 0. 001 ). The ROC curve showed that the LN area index as a diagnostic indicator had high sensitivity and specificity. The LN area index showed a significant correlation with HCV-Ag level (r = 0.436, P <0.05), but no significant differences were found between LN area index and HCV-Ag grade. Conclusions Perihepatic lymphadenopathy indicates viraemia,and LN area index of 100 mm2 is a reference index in estimating whether patients have HCV infection.

[Objective]To assess the safety and clinical result of Osteoset artificial bone and autogenetic bone grafting ccmbined with internal fixation after radical debridement in the treatment of spinal tuberculosis.[Method]From April 2004 to May 2006,24 cases with spinal tuberculosis were treated with radical debridement,Osteoset artificial bone and autogenetic bone grafting combined with internal fixation.Radiographs(X-rays and CTs) were analyzed to access bony fusion and deformity correction.[Result]All the patients acquired satisfactory incision healing without fever.The follow-up period ranged from 6 to 24 months(mean 15 months).There was no recurrence of the disease or loose instrumentation.The average Cobb's angle correction was(15.2??6.7?).The rate of fusion was 95.8% 3 month after operation,while it was 100% 6 month after operation.[Conclusion]Osteoset artificial bone can avoid the complications of donor bone site.The early clinical outcome is satisfying.

Objective Investigate the clinical features of the neurological disorders in patients after pulmonary thromboendarterectomy (PTE) for chronic pulmonary thromboembolism , analyze the factorial. Methods A retrospective study was made of 26 patients who underwent PTE between 2002 and 2010 in Beijing Chao-Yang hospital. The symptom of neurological system disorders occurred in the survivors were been investigate. The preoperative conditions and the perioperative conditions of all survivors were investigated. Compared the differences between the groups with neurological system disorders and the groups without. Results There were 22 patients of 26 survived after the surgery. Cardiac function of the survivors improved, and the quality of life improved significantly. 5 cases of the 22 survivors had symptoms of neurological system disorders. 3 patients showed lethargy, delirium, memory disorders, brain CT found no abnormal, symptoms recovered within 2 weeks. The fourth patient showed ataxia, unsteady gait, dance-like movements symmetry, in addition to the above symptoms, brain CT no abnormal showed,brain MRI showed bilateral abnormal signal in the midbrain, basal ganglia, symptoms improved and discharged after 8 weeks, the symptoms completely disappeared 6 months, and the abnormal signals in brain in MRI disappeared. The fifth patient with similar symptoms to the fourth, brain CT no abnormal found, be discharged 10 weeks after the operation, recovery is poor, living part of themselves. The postoperative neurological disorders occurred in the patients of Jamieson surgery type Ⅲ type, Ⅳ type of larger proportion, (P = 0.024), longer circulatory arrest surgery (P = 0.034). Conclusion The neurological disorders postoperative PTE often showed diffuse symmetric cerebral cortex and basal ganglia nerve dysfunction, brain MRI showed abnormal signal corresponding region, the majority of symptoms disappeared in 2 - 8 weeks, abnormal signal in brain MRI could disappeared after 6 months. Neurological dysfunction occurs in patients with more difficult surgical procedure, longer circulatory arrest, suggesting that with the surgery cerebral ischemia and hypoxia related.

Objective To evaluate the value of predicting functional improvement of viable myocardium after percutaneous transluminal coronary angioplasty(PTCA) by strain rate imaging(SRI) combining with low-dose dobutamine stress echocardiography(LDDSE).Methods PTCA was performed in 20 patients with old myocardial infarction and 72 segments of resting dyskinetic wall motion at baseline were observed.Peak systolic strain rate(SSR) and systolic peak velocity(Vs) were measured in abnormal walls at baseline and SRI combined with LDDSE one day before PTCA.Increment of SSR and Vs was defined during stress echocardiography(?SSR% and ?V_S%).The follow-up analysis was performed 6 weeks after PTCA.Results The dyskinetic motion of 48/72 segments was improved according to the follow-up echocardiography 6 weeks after PTCA while that of 24/72 segments was not.Considering ?SSR%≥(2.02%),?V_S%≥(8.5%) in 5 ?g?kg~(-1)?min~(-1) stress and ?SSR%≥(24.7%),?V_S%≥(28.5%) in 10 ?g?kg~(-1)?min~(-1)stress as cut-offs for motion improvement of the left ventricular dyskinetic segments,the sensitivities were(93.8%),(89.6%) and(95.8%),(91.7%),respectively.At baseline SSRs of two groups were significantly different while Vs did not have significant difference.Conclusions Combined with LDDSE,SRI can more accuratly predict the motion improvement of viable myocardium than tissue velocity imaging can.

ObjectiveTo explore the relationship between the impairment cognitive function and the location and number of infarcts.MethodsThe composites the scores for memory,processing speed and executive function were detected using the neuropsychological measuring scales and determined the cortical,subcortical and cerebral infarcts by magnetic resonance imaging( MRI),then analyzed the relationship between the impairment cognitive function and the location and number of infarcts.ResultsCompared to the control group,the patients with infarcts in multiple locations had poorer memory(the score of AVLT-5 was 6.14 ± 1.89 and 7.26 ± 1.82,respectively,P ＜ 0.01 ).Compared to the patients without infarcts,a combination of cortical and subcortical infarcts was associated with slow processing speed.The patients with multiple infarcts in multiple locations had significantly lower composites scores for memory(the score of LMT-delay was 10.86 ± 3.48 and 12.69 ± 2.60,respectively,P＜ 0.01 ),processing speed ( the score of SDMT was 27.08 ± 11.13 and 34.23 ± 7.44,respectively,P ＜ 0.01 ) and executive function( the score of TMT-B was 1.34 ± 0.65 and 0.40 ± 0.35,respectively,P ＜ 0.01 ) compared to control group.ConclusionThe multiple infarcts in multiple locations is associated with the impairment cognitive function in patients with cerebral infarcts.Suggesting that both the location and the number of infarcts jointly contribute to cognition impairment.

Objective To explore the relationship among left ventricular(LV) peak systolic strain rate,the mitral annular motive amplitude(MAMA) and left ventricular ejection fraction(LVEF) in essential hypertension patients with different left ventricular patterns.Methods According to level of LV mass index and relative wall thickness,40 patients with hypertension were divided into patterns of normal geometric LV(LVN) and remodeling LV(LVR),20 normal patients were on control group. The apical views were used to determine MAMA and peak systolic strain rate(SSR).MAMA was determined by anatomic M-mode(AMM) and strain rate imaging was applied for obtaining SSR of LV basal and middle segments.LVEF was determined by Simpson rule.The LV systolic function was evaluated by mean of MAMA,SSR and LVEF. Results In all subjects,mean SSR by strain rate imaging of LV wall correlated negatively with mean MAMA(r=(-0.91),P(0.05)).Conclusions Strain rate imaging provides a sensitive,simple and trust tool to assess globle LV function in patients with essential hypertension.

Objective To investigate serum uric acid (UA) levels and related clinical features in patients with high risk syndrome of neuromyelitis optica. Methods UA levels were measured in 51 patients with high risk syndrome of neuromyelitis optica including 34 with longitudinally extensive transverse myelitis (LETM) and 17 with optic neuritis (ON), 48 with neuromyelitis optica (NMO), 45 with other neurological diseases (OND) and 65 with healthy controls (HC). The disability severity was assessed by the expanded disability status scale (EDSS). Spinal lesions were viewed by MRI. Serum aquaporin-4(AQP4) antibody was tested in cell based immunofluorescence assay. Results Serum UA levels in LETM ( ( 189. 84 ±85. 65) μmol/L) and ON patients ( (222. 12 ±61.68) μmol/L) were significantly lower than that in OND ((315.90±71.36) μ mol/L) and HC ((291.05 ±76.64) μ mol/L) subjects (P＜0.01). No difference was found between LETM, ON and NMO groups. UA levels were significantly lower in females ( ( 158.24 ±55.92), (187.00±47.52), (198.21 ±62.62), (274.51 ±70.66)and (243.26±60.65) μmol/L)than in males ( ( 262. 09 ± 101.63 ), ( 262. 45 ± 62. 13 ), ( 298.90 ± 74. 14 ), ( 355.37 ± 50. 30 ) and (340. 34 ±58. 23) μmol/L) in all groups (t=3. 183, 2.578, 4.356, 4.365 and 6.579, all P＜0.05).UA levels in patients with high risk syndrome of NMO were not correlated with mono or relapse course,duration or status of serum AQP4 antibody. UA were negatively correlated with EDSS in patients with LETM (r= -0.714, P＜0.01). Conclusion Lower serum UA levels were found in patients with high risk syndrome of NMO and related to more severe symptoms in LETM group.