To evaluate effects of health education on treast-feeding, studies have been conducted on 203 gravidas who were delivered of their babies in hospital. In the inteervene group, the rate of exclusively breastfeeding in the period of early sucking lates and breast-feeding according to the necessity and during the post6partum four months is higher than that of the control group, while the neonate's weight-reduction in the intervene group is lower than that of those in the control group when they leave hospital. The disparity is significant after dealt with by statistics. The study suggests that health eeeducation is on important and indispensable measure to develop breast-feeding.

Objective To investigate the effects of pioglitazone on serum adipocytokines (leptin, resistin and adiponectin) in polycystic ovary syndrome (PCOS) patients with insulin resistance (IR). Methods Thirty-five PCOS patients with IR were treated with pioglitazone 15mg/d for 12 weeks. The results of ovulation induction were observed. The changes of fasting plasma glucose (FPG), fasting serum insulin (FINS), serum levels of leptin, resistin and adiponectin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and blood fat were examined at the baseline and after the therapy by enzyme-linked immunosorbentassay (ELISA) and radioimmunoassay (RIA). Results After 12 weeks' treatment, menstruation and rate of ovulation per cycle were improved in 35 (88.5%) PCOS patients with IR. Waist/hip ratio and F-G score were significantly decreased (P<0.05), and BMI declined with no significant difference (P>0.05). The levels of LH, T, FINS, HOMA-IR, total cholesterol, triglyceride and low density lipoprotein-cholesterol were significantly decreased (P<0.01, P<0.05) after treatment; high density lipoprotein-cholesterol level was significantly increased (P<0.01) after treatment. However, there were no significant differences in FSH and FPG (P>0.05). The levels of serum leptin and resistin were decrease than before (P<0.05), and the level of serum adiponectin was increased (P<0.05). Conclusion Pioglitazone can effectively improve clinical syndromes, insulin sensitivity, glucose and lipid metabolism of PCOS patients with IR. Adipocytokines (leptin, adiponectin and resistin) as regulators of insulin metabolism are involved in the pathogenesis of insulin resistance in PCOS. Pioglitazone treatment can decrease plasma glucose level and improve insulin sensitivity at least partly through improving the profiles of adipocytokines.

Objective To investigate the expressions and clinical significance of PPAR? and MMP-9 in the genesis and development of hypertensive disorder-complicating pregnancy(HDCP).Methods The expressions of PPAR? and MMP-9 in placenta of 31 normal pregnant women(control group) and 36 pregnant women with hypertensive disorder-complicating pregnancy(experimental group) were detected by using SP method of immunohistochemistry,and the relationship between PPAR? and MMP-9 expressions and hypertensive disorder-complicating pregnancy was explored.Results ① PPAR? expression in placental tissues was significantly higher in the experimental group than in the control group(P

The work that the reformation of worker's medical insurance system is being put into effect is both a oppotunity and a challenge for the development and reformation of our hospital. There is a lot of things to do in the hospital, but dealing seriously with medical moral standars formation of the hospital has a role of guiding and protecting for other works to do well. Therefore, the related sections of hospital have to regard it as a important thing.

Objective To investigate the expression of H19 imprinted genes in the villus tissues of women with spontaneous abortion.Methods Reverse transcription-polymerse chain reaction method(RT-PCR) was employed to detect H19 imprinted genes allele-specific expression levels of the villus tissues extracted from 45 cases of spontaneous abortion and 30 cases of normal pregnancy.Results Nineteen cases of 21 heterozygous cases in spontaneous abortion samples showed billelic expression of H19(19/21),whereas no billelic expression was found in the normal pregnancy samples(0/13)(P

Objective To investigate the effects of pioglitazone on serum leptin and adiponectin in polycystic ovary syndrome (PCOS) patients with insulin resistance (IR). Methods Thirty-five PCOS patients with IR were treated with pioglitazone 15mg/d for 12 weeks. The results of ovulation induction were observed. The changes of fasting plasma glucose (FPG), fasting serum insulin (FINS), serum levels of leptin, adiponectin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and blood fat were examined at the baseline and after the therapy by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Results After the treatment, the rate of ovulation per cycle was improved in 31 (88.5%) out of the 35 patients. After treatment, the level of serum leptin was decreased (P<0.05) while the level of serum adiponectin was increased (P<0.05). After 12 weeks'treatment, waist-to-hip ratio and F-G score were significantly decreased (P<0.05), BMI declined but without significant difference (P>0.05). The levels of LH, T, FINS, Homa-IR, total cholesterol, triglyceride and low density lipoprotein-cholesterol were significantly decreased (P<0.01, P<0.05), whereas the level of high density lipoprotein-cholesterol was significantly increased (P<0.01). No significant difference was seen in FSH and FPG following treatment (P>0.05). Conclusion Pioglitazone treatment can effectively improve PCOS with IR patients'clinical syndromes, insulin sensitivity, glucose and lipid metabolism at least partly through improving the profiles of leptin and adiponectin.

Objective To construct the plasmid pSilencer4.1/HtrA1 and stably transfect human trophoblastic cell line HPT-8.Methods We detected the expression of HtrA1 in cell line HPT-8 with immunohistochemical SABC,constructed the plasmid pSilencer4.1/HtrA1,transfected human trophoblastic cell line HPT-8 with plasmid pSilencer4.1/HtrA1 and negative plasmid pSilencer4.1, and observed the cell expression after transfection. Results About 90% of cellular cytoplasm of HPT-8 was dyed brown while the nucleus was negatively stained. Selective concentration of G418 was 200 μg/mL for HPT-8 to be transfected pSilencer4.1/HtrA1.With RT-PCR and Western blot methods,the expression of HPT-8 transfected plasmid Psilence4.1/HtrA1 was significantly downregulated compared with that of cells with negative plasmid and normal cells (P < 0.05 ).There was no significant difference in absorbance value between blank plasmid group and HPT-8 group (P >0.05).Conclusion We successfully constructed stable trophoblastic cell line HPT-8/ pSilencer4.1-HtrA1 with silenced expression of HtrA1,which lays foundation for further research.

Objective To evaluate the effects of nimodipine (NIM) on pregnancy induced hypertension(PIH) and to assesse the effects of nimodipine on intraplatelet free calcium(PF[Ca 2+ ] i). Methods Fourty eight patients with moderate or severe PIH were randomized to receive either NIM or magnesium sulfate. We observed the change of blood pressure, urc protein, PF[Ca 2+ ] i and symptoms of preeclampsia before and after medication. We compared the pregnancy outcome of the two groups. Results 1.There was significant reduction of blood pressure at 1/2 h?1 h and 2 h after the administration of nimodipine. The maternal heart rate increased significantly ( P

Objective To study the relationship between fetal growth retardation(FGR) and expression of insulin-like growth factor-2 and H19 imprinted genes.Methods Immunoradioassay was used to detect the insulin-like growth factor-2(IGF2)level in cord blood from 42 pregnant women with FGR(FGR group) and 30 normal pregnant women(normal group).Reverse transcription-polymerase chain reaction method(RT-PCR) was used to detect H19 imprinted genes allele-specific expression level in placenta.Results The level of IGF2 in cord blood from FGR group was(1.52?0.20)?g/L.It was significantly decreased as compared with that from the normal group(1.97?0.21)?g/L(P

Objective To explore the therapeutic effects of nimodipine and magnesium sulfate on severe pregnancy induced hypertension (PIH) as well as the effect on plasma endotheline (ET) and serum nitric oxide (NO), in order to provide theoretical foundation for clinical treatment. Methods Forty two patients with severe PIH, being divided into 2 groups at random, were treated with nimodipine or magnesium sulfate. The changes of blood pressure, arteriole of eyeground, urine protein, ET, NO and clinical symptoms were observed respectively before and after the treatment, and the pregnancy outcomes were compared. Results (1) After the treatment of nimodipine, the serum NO of patients increased significantly from (51.72?14.64)?mol/L to (67.56?14.77)?mol/L ( P 0.05). (2) The nimodipine group took shorter time (0.5 h) to lower the blood pressure notably than the magnesium sulfate group (2 h).(3) As for the disappearing of subjective symptoms , nimodipine group (1.7? 1.3) h was quicker than magnesium sulfate group (3.4?1.7) h. Conclusions Compared with magnesium sulfate, nimodipine was better in improving and protecting the function of endotheline cells, dilating cerebral blood vessels, depressing blood pressure strongly and persistently, reducing subjective symptoms of patients and had no other side effects except increasing heart rate.