Objective To analyze the change trend of drug resistance in clinical strains of Escherichia coli,and to provide the basis for rational use of antibiotics in clinic,and to increase the level of drug resistance.Methods The drug resistance of Escherichia coli isolated from various clinical specimens in hospital from January 2012 to November 2016 were analyzed retrospectively.Results From all kinds of clinical specimens 3 458 strains of Escherichia coli were isolated,extended spectrum beta-lactamases producing rate of which was 57.92％.The drug resistance rates of Escherichia coli to Quinolones,Penicillins,Cephalosporins,Aminoglycosides,Sulfonamides were more than 50.00％;The resistance rate of Imipenem,Meropenem,Piperacillin/Tazobactam,Cefoperazone/Sulbactam and Cefoxitin were less than 10.00％ of the antibacterial drugs,the resistance to some third,forth generation Cephalosporins were significantly increased.The Wilcoxon test showed that drug resistance rate of Escherichia coli isolated from sputum was significantly higher than that of blood,urine,secretions,the difference was statistically significant(P<0.05).Conclusion Clinicians should attach great importance to which the drug resistance of Escherichia coli in a hospital increased year by year.

AIM: To investigate the anti-apoptotic effect of hepatocyte growth factor(HGF) on actinomycin D(ActD)-induced apoptosis in hepatocytes and the possible pathway.METHODS: Hepatocytes were exposed to ActD and HGF.The cytotoxic effects of ActD were tested by MTT.Apoptotic cells were identified by Hoechst 33342 staining,flow cytometry and detection of DNA fragmentation with agarose gel.Akt and phospho-Akt were detected by Western blotting analysis.RESULTS: The results showed that ActD induced apoptosis in hepatocytes 5 h after treatment.Phosphatidylinositol-3 kinase(PI-3K) specific inhibitor wortmannin enhanced the apoptotic effect of ActD.Furthermore, HGF significantly reduced the apoptosis in hepatocytes induced by ActD in a concentration-dependent manner.In the presence of wortmannin,HGF did not overcome apoptosis.CONCLUSION: Wortmannin enhances the apoptotic effect of ActD.HGF protects hepatocytes from apoptosis induced by ActD through a PI3K/Akt pathway.

Objective To evlauate the effect of pulmicort respule combined with solution salbutamoI aerosol on the treatment of bronehiolitis.Methods 65 cases of bronchioiltis were randomly divided into two groups.solution saltutamol inhalation group or pulmicort respule combined with solution salbutamoI group,the time of easing asthma symtpoms was assessed.Results Therapeutic group cure rate is higher than the control group(87.9%/62.5%,P＜0.01),there is significant difference.Observation group duration of symptoms was significantly shorter than the oontrol group(P＜0.01),the difference is significant.Condmlon Pulmicort respule combined with solution salbutamol has significant therapeutic effect on the treatment of bronchiolitks,there are obvious advantages.

Objective To discuss the clinical manifestations and the morphological features of intracranial arteriovenous malformation (AVM) in childhood. Methods During the period from Jan. 2012 to June 2013, a total of 14 AVM patients under the age of 14 years were admitted to authors’ hospital. Of the 14 patients, 11 had a history of spontaneous intracerebral hemorrhage (78.6%), 7 complained of headache (50.5% ), 5 showed signs of unilateral limb weakness (35.7% ), and 2 was accompanied with epileptic attacks (14.3% ). The clinical characteristics, the preoperative imaging features and DSA findings were retrospectively analyzed. Results DSA showed that terminal feeding branches were visualized in 9 cases and blood supply from perforating branches was seen in the remaining 5 cases. In seven cases the feeding artery was single, while in the remaining 7 cases the feeding artery was multiple, and in four of them combination blood supply from both anterior and posterior circulation was found. The lesions were situated at the lateral or convex surface of cerebral hemisphere (n = 5), lateral ventricle (n = 4), cerebral bottom (n = 3) or medial surface of cerebral hemisphere (n = 2). The lesion’s diameter was < 30 mm in 10 cases and (30 -60) mm in 4 cases. According to Spetzler- Martin grading, grade Ⅰ was seen in 4 cases, grade Ⅱ in 2 cases, grade Ⅲ in 5 cases and grade Ⅳ in 3 cases. Conclusion Although in childhood AVM lesion is small in size and lower in grading, its onset is early and the lesion is prone to bleeding. DSA findings indicate that most AVMs in childhood take the form of immature type. Nevertheless, its natural course needs to be further studied.

ObjectiveTo discuss neonatal hypoxic-ischemie eneephalopathy points,and the vesting of the re- lationship between the prognosis and current treatment methods. MethodsFrom January 2004 to April 2008, pedi-atric hospital treated 88 cases of HIE were assessed, inspected, grading and treated. Results8 cases in the 88 easesantomatically discharged, 51 patients (63.8 %) were recovered and 28 patients (35.0 %) improved, and 1 patient(1.3 %) died. HIE clinical severity has close relationship with prognosis. Mild and moderate cases were 78.4 % (29/37) ,53.7% (22/41) were recovered and no deaths, and severe 1 case, 1 death. ConchtsionHIE clinical severityand prognosis have close relationship, the time of birth asphyxia situation on the vesting of infants discharged andwas no significant impact.

Laser has been used in the treatment of glaucoma for more than twenty years.Its main applications include laser peripheral iridoplasty,laser trabeculoplasty,laser sclerostomy,and laser cyclophotocoagulation. Other applications such as C02 laser filtering surgery and photodynamic therapy(PDT)have just started.In this paper,a review is provided on the present applications of lasers in the treatment of glaucoma including choice of laser,indications,surgery approach and efficacy.The prospect of the future application is also discussed.

Objective To compare the efficiency of χ~2-Fisher's exact test which is one of the competitive null hypothesis approaches with SAM-GS which belongs toself-contained null hypothesis approaches. Methods The two methods were used to analyze a simulation experiment which contained five different scenarios. The results were compared with the simulated initialization,and assessing indexes were calculated to compare the efficiency. Results Under the same conditions,SAM-GS always have higher power than that of χ~2-fisher's exact test. However, the final inference is equivalent, namely if the difference between the two groups are smaller than 0.30,the two methods can not be better to identify differences between them. By contrary, when the differences between the two phenotypes are larger than 0.30, the two ways can both identify differences. Conclusion SAM-GS tends to have slightly higher power thanχ~2-Fisher' s exact test. The two methods can be used for screening enrichment gene sets of gene expression profile.χ~2-Fisher's exact test has the important advantage of being able to analyze multi-class phenotype.

Objective　To study the characteristics of serious central nervous system(CNS) involvement in childhood systemic lupus erythematosus.Methods　We made a comparison on the level of ANA、dsDNA and positive rate of Sm、C3 between primary and secondary CNS involvement and analysed the clinical manifestations between two groups.Results　The level of ANA、dsDNA and ositive rate of Sm、C3 were not related with SLE encephalopathy;EEG was useful to the diagnosis of SLE.Conclusion　The differiential diagnosis between primary and secondary CNSD in volvement of SLE must be analysed according to clinical manifestations and other laboratory findings.

Objective To determine the serum level of Visfatin, and observe the effect of short-term treatment of rosiglitazone on it in the patients with type 2 diabetes mellitus. Methods Thirty-three patients with type 2 diahetes mellitus(diabetic group) who were inadequately controlled by suffonylureas were treated with rosiglitazone (4 mg/d) for 12 weeks. Twenty-seven sex-, age- and BMI-matehed non-diabetic subjects (control group) were studied. Determined blood pressure, Visfatin, triglyceride (TG), high density lipopro-tein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG),2-hoar postprandial glucose (2 h PPG), fasting insulin (FINS)in all subjects and free fatty acid (FFA), high sensitive C-reactlve protein (hs-CRP) and glyeosylated hemoglobin (GHbA1c), abdominal fat distribution before and after treatment with rosiglitazone in diabetic group respectively. Results The level of serum Visfatin was lower in diabetic group than that in control group [(24.53±9.22) μg/L vs (53.10±27.00)μg/L, P<0.01 ], and it was higher after treatment with rosiglitazone for 12 weeks, but there was no significant difference [(24.53±9.22)μg/L vs (32.62±24.89) μg/L, P>0.05]. Visfatin was correlated significantly with systolic pressure (r =-0.29, P=0.02), FPG (r=-0.40, P=0.001 ), 2 h PPG (r=-0.34, P=0.009 ),LDL-C (r=0.26,P=0.049) and FFA (r=0.44,P=0.009) respectively. In a linear stepwise regression analysis, FFA showed significant correlation with serum Visfatin levels (r2=0.30,f=7.707, P=0.009).Conclusions The level of serum Visfatin is lower in type 2 diabetes mellitus, and it is related significantly with glucose and lipid metabolic factors. The short-term treatment with rosiglitazone can't increase the serum concentration of Visfatin in the type 2 diabetes mellitua.