Objective To investigate the changes of the pulmonary surfactant protein-D (SP-D) in serum and cerebrospi-nal lfuid in children with viral encephalitis (VE). Methods The levels of SP-D in serum and cerebrospinal lfuid were detected by a double-antibody sandwich enzyme-linked immunosorbent assay and compared in thirty children with VE in acute and con-valescent phases and in 12 children without VE. Results The levels of SP-D in serum and cerebrospinal lfuid between groups of VE acute phase and convalescent phase and no VE were statistically signiifcant (F=103.58,118.15, all P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in acute phase and in convalescent phase were signiifcantly lower than children without VE (P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in convalescent phase were all signiifcantly higher than those in acute phase (P<0.01). In children with VE, the level of SP-D in cerebrospinal lfuid was weakly correlated negatively with the count of nucleated cells. Conclusions SP-D might be involved in the pathogenesis in VE. The detection of SP-D in serum and cerebrospinal lfuid has a certain value for diagnosis of VE.

The prevalence of childhood primary hypertension is increasing each year,not only bring-ing symptoms to these children,but also causing damage to multiple target organs such as heart,kidney,blood vessels,eyes and nerve system. Childhood hypertension is a risk factor of adult hypertension. Paying atten-tion to and preventing childhood hypertension has become a public health challenge. There are many associat-ed risk factors for childhood primary hypertension,including parental hypertension,overweight and obesity, low birth weight,and so forth. From the analysis of these factors,suggestions can be given about how to pre-vent childhood primary hypertension.

Objective To observe the clinical efficacy of Shenfuyishen Capsule in the treatment of diabetic nephropathy of yangxuxueyu type in high- altitude regions.Methods We divided 53 patients randomly into two groups:31 in the observation group and 22 in the control group.Patients in the control group were treated with conventional regime of Western Medicine.Those in treatment group were given extra treatment with Shenfuyishen Capsule.Then we tested serum creatinine (Scr ),uric acid (UA),urinary microalbumin (MALB )and GFR. Results The total efficiency was 90.32% and 81.82% in the treatment group and the control group,which was significantly different (P < 0.05 ).Major symptoms improved after treatment in both groups,which differed significantly compared with those before treatment (P <0.05).ALT in the treatment group was not different after treatment.CR,UA,MALB and GFR significantly differed before and after treatment in both groups,especially in the treatment group (P <0.05).Conclusion Shenfuyishen Capsule helps improve major clinical symptoms and decrease CR,GFR,UA and MLAB levels in patients with diabetic nephrology,thus protecting renal function and delaying the process of diabetic nephropathy.

Phosphatase of regenerating liver-3 (PRL-3) is a novel small molecule protein-tyrosine-phosphatase,which plays an important role in the oncogenesis and deveopment of tumors.Studies show that PRL-3 regulates neoplasm progress through participating in multiple signal pathways.Its high expression can significantly promote neoplasm metastasis in cancer tissue.However,there is no expression of PRL-3 in most normal tissue.PRL-3 is expected to be a potential tumor maker of diagnosis and a new target for the therapy of cancer.

Objective To explore the community nursing talents' occupation quality demand in the purpose of periodically providing reference for higher medical institutions' nursing education and for job training of community nurses.Methods The community nurses in the 15 qualified communities' medical organizations in the city of Zunyi were investigated through self-designed questionnaires,analyzing the basic qualifications,humanistic knowledge,professional knowledge and profession ability demand that community nursing talents were supposed to possess.Results The demand of high occupation responsibility and strict working style amounted to a relatively large proportion among the 11 basic qualifications.Demand of nursing etiquette and community nursing management occupied a relatively large proportion among the 9aspects of community nurses' humanistic knowledge demands.Demand of the often used community nursing technical operation and common drugs' clinical application knowledge took up a relatively large proportion among the 16 community nurses' professional knowledge demands.Demand of interpersonal and communication skills and self protection ability held a relatively large proportion among the 14 community nursing talents' occupation ability demands.Conclusions Oriented to the demand of the community nursing talents' occupation quality demands,relevant higher medical institutions or colleges concerned should strengthen the basic quality,optimize the curriculum structure and improve occupation ability in the purpose of culturing the community nursing talents who are able to adapt to the demand of community health service and have higher overall qualities as well.

Objective: To syudy the pharmacologic and clinical effects of Jiaweizhiyu Decoction.Methods: The decoction for relieving pain, reducing blood sugar and controlling thrombus formation was investigated.Results: Jiaweizhiyu Decoction has effects on relieving pain, reducing blood sugar and controlling thrombus formation.Conclusion: It will be further researched for us.

OBJECTIVE:To evaluate clinical efficacy and the effect of pharmacoeconomics of two chemotherapeutic regimens for the first line treatment of advanced gastric cancer. METHODS:98 patients with advanced gastric cancer receiving SOX or FLO regimen as first line chemotherapy in our hospital were enrolled. According to chemotherapy grouping,short-term efficacy,toxic re-action and pharmacoeconomics effect were analyzed and compared between 2 groups. RESULTS:There were a total of 55 patients in SOX group,with effective rate of 38.18%;43 patients in FLO group,with effective rate of 32.56%;there was no statistical sig-nificance in effective rate between 2 groups(P>0.05). There was no statistical significance in toxicities between 2 groups,includ-ing nausea and vomiting,neurotoxicity and neutropenia(P>0.05). The total cost of FLO regimen group was 9 136.98 yuan,while that of SOX regimen group was 12 345.97 yuan. Using minimum cost method,FLO regimen was superior to SOX regimen regard-ing the economical index. CONCLUSIONS:SOX and FLO regimen have no significant difference for the first-line treatment of ad-vanced gastric cancer in terms of response rate and toxicities. Taking into account the parmocyeconomics,FOL regimen is superior to SOX regimen.

Objective to investigate the efficacy of functional electrical stimulation(FES)on the postoperative treatment of lumbar disc hernia-tion. Methods Informed consents were obtained from all the subjects before the study. totally 60 patients who had remnant nervous lesion after sur-gery of lumbar disc herniation were divided into two groups:FES group and Control group. FES group received treatment since the first day after sur-gery. the parameters of therapeutic apparatus were as follows:35 Hz,0.28 ms,1 time daily and 30 min each time. the intensity of the treatment was the maximum tolerated that the patients can take. the course was 4 weeks. the control group during the study did not receive any stimulation. the myodynamia of hallux dorsiflexion and JOA score of patients were recorded and analyzed. Results After 2 weeks treatment,the myodynamia of FES group was 15.10±5.03,which was significantly larger than that of the control group at 12.03±6.30(P < 0.05). After 4 weeks treatment,the myody-namia of FES group was 20.43±5.73,which was significantly larger than that of the control group at 14.57±7.17(P < 0.05). After 4 weeks of treat-ment,the JOA score of FES group was 24.63±1.91,which was significantly higher than that of the control group at 19.87±2.33(P < 0.05). Conclu-sion After 2-4 weeks of functional electrical stimulation therapy,myodynamia of hallux dorsiflexion in FES group was significantly improved. And at the 4th week after operation,JOA score was more higher than that of the control group. Functional electrical stimulation in the treatment of remnant nervous lesion after postoperative of lumbar disc herniation has exact curative effect,which is worthy of popularization.

Objective To determine the effects of different excipients ( amino acids, carbohydrates and nonionic surfactants) on thermal stability of the IgG1 monoclonal antibody, and to examine the interactions between the excipients and the protein.Methods Differential scanning calorimetry ( DSC) was used to study thermal stability of the protein in different solutions and got information on the solubility of the unfolded forms of the protein.Isothermal titration calorimetry ( ITC) was used to examine the binding interactions between the excipients and the protein.ResuIts Negatively charged amino acids could significantly reduce the denaturation temperature (Tm) of IgG1( Tm >9 ℃), and other excipients didn’t have a major effect ( Tm <1℃).Excipients shared different impacts on thermal stability of the IgG1 monoclonal antibody under different pH, and negatively charged amino acids result in a much lower Tm at pH 5 than at pH 7.The ITC binding isotherms of different excipients (including polysorbate 20 and 80) and IgG1 were almost straight lines, while there was strong binding interaction between polysorbate 20 or 80 and Human Serum Albumin (HSA).ConcIusion The results suggest that there is no binding interaction between these studied excipients and the IgG1 monoclonal antibody; instead electrostatic interactions seem to play a leading role between the excipients and the IgG1 monoclonal antibody.

Objective To study the influence of RNA interference Caspase-3 gene on apoptosis of transformed human embryonic kidney 293 cells induced by cadmium in vitro. Methods Transformed human embryonic kidney 293 cell were treated with siRNA for 36 h. The treated cells were incubated with 40 ?mol/L CdCl2 for 12-24 h and the cells viability were measured with methyl thiazolyl tetrazolium(MTT) assay. In addition,the expression of caspase-3 gene in 293 cells was detected by the methods of reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis,and the occurrence of apoptosis was determined by flowcytometry with Annexin-V and propidium iodide (PI) double labeling method. Results The results of RT-PCR and Western-blot analysis revealed that in incubated cells with 40 ?mol/L cadmium 12 h,the expression of Caspase-3 mRNA and 20 kDa protein significantly increased when compared with controls(n=4,P