AIM: To study the expressions and significance of stem cell factor(SCF) and tyrosine kinase receptor(KIT) in neurons after cerebral ischemia reperfusion injury.METHODS: Western blotting was used to examine the expression of SCF and KIT in the cortex after cerebral ischemia and oxygen glucose deprivation(OGD) neurons,then the OGD neurons preincubated with SCF and SCF-Ab were assessed the cell viability by MTT to determine its significance.RESULTS: ①Compared to control group,the expressions of M-SCF and S-SCF in cortex increased markedly while KIT decreased significantly in ipsilateral hemisphere.However,no obvious change in the contraleteral hemisphere was observed.②Compared to control group,the levels of S-SCF and M-SCF were increased significantly in the OGD neurons while KIT was not obvious changed.③The cell viability of OGD neurons decreased significantly.Preincubation with SCF inhibited cell damage while SCF-Ab deteriorated OGD injury.CONCLUSION: The expression of SCF may be induced by cerebral ischemia reperfusion,which can protect the neurons from ischemia injury.

Objective To establish a method for the determination of methyl tertiary butyl ether (MTBE) in the cerebrospinal fluid of rats by gas chromatography and to know whether MTBE can penetrate the blood brain barrier. Methods 9 male SD rats were randomly divided into 3 groups and then exposed to MTBE by gavage at a dose of 1000 mg/kg?bw and by inhalation at a dose of 8 ml/L for 30 days. The cerebrospinal fluid was collected at 6 h, 12 h and 24 h respectively at the end of treatment and the MTBE in rats cerebrospinal fluid was determined by gas chromatography. Results MTBE was detected in the cerebrospinal fluid of rats after subchronic exposure of MTBE through gavage and inhalation. Conclusion MTBE is able to penetrate the blood brain barrier and reach the brain.

Objective To investigate air quality in the basements of high science-technologic buildings. Methods Continuous tests of air quality parameters of physics, chemistry and microbiology were conducted in 11 rooms of the basement used for working and living. Results At every test point the average room temperature was 25.5 ℃, the average relative humidity was 78.7 per cent, the average wind velocity was 0.057 m/s. Between the relative humidity and temperature(r=-0.850, P

Objective To study the prognosis of polymyositis(PM) and dermatomyositis(DM) patients and its influence factors.Methods Sixty DM and 30 PM patients diagnosed according to the diagnostic criteria of Bohan and Peter in our hospital during 2000-2008 were used as study subjects.The patients were followed up till their death or to August 2009.Gender,age of disease onset,disease course,serum creatine kinase,interstitial lung disease,heart involvement,connective tissue disease(CTD),malignancies,and treatment with corticosteroids,immunoglobulin and immunosuppressive agents were assessed as predictive factors for the prognosis of patients.Characteristics of muscular biopsy from 20 cases were analyzed.Results The median age of the 90 patients(29 males and 61 females) was 51 years(range 6-74 years).The male and female ratio was 1∶2.The most commonly involved muscles were the proximal muscles of limbs(83.3%),followed by neck muscles(25.6%),laryngea pharyngeal muscles(12.2%) and masticatory muscles(2.2%).Among the 42 patients(46.7%)with lung disease,interstitial lung disease and hear involvement were found in 29(32.2%)and 13(14.4%)patients,respectively.Of the 13 patients complicated by connective tissue disease,DM and PM accompanying connective tissue disease were diagnosed in 9 and 4,respectively,and DM and PM accompanying malignancies were observed in 2 patients.Muscular or skin biopsy was performed for 23 patients,which showed typical inflammatory infiltration in 13,dermatomyositis in 3,and no significant lesion in 2 patients,respectively.Of the 18 patients who died during the follow-up,5(16.67%) and 13(21.67%) died of PM and DM,respectively.Seventy-two patients survived.Their 1-,5-,and 9-year survival rate was 90%,84.4%,and 80%,respectively.The complete and partial remission rate was 22.2% and 36.7%,respectively,with a relapse rate of 20%.Advanced age of disease onset(P=0.003 8),interstitial lung disease(P=0.011 3) and malignancies(P=0.004 9) were main causes of death.Malignancy(RR=6.34,P=0.001 2)was the factor for poor prognosis and long-term treatment with hormones and immunosuppressive agents is the protective factor for PM and DM.Conclusion Complete and partial remission can be achieved in 58.9% patients with DM and PM.Advanced age of disease onset,interstitial lung disease and malignancy are the factors for poor prognosis of such patients.Long-term treatment with corticosteroids and immunosuppressive agents are the protective factors.

Objective To observe the clinical features of AIDS coinfected with cryptococcus meningitis. Methods The clinical data of 19 AIDS patients coinfected with cryptococcus meningitis were retrospectively analyzed.Results Among 19 patients, the main clinical manifestation including headache (100.0%), nausea/vomiting (94.7%), fever (78.9%) and neck stiffness (84.2%).Seven cases revealed increased CSF protein, 10 cases showed depressed CSF glucose levels and 11 patients revealed elevated CSF pressures.The mean CD4+cell count was (58.9 ±27.8)/mm3.Imaging examination showed the neurological complications were hydrocephalus, cerebral infarction, cerebral atrophy and cerebral hernia.The usage rate of amphotericin B, amphotericin B liposome, fluconazole and fluorine cytosine was 31.6%, 21.1%, 47.4% and 47.4%, respectively.Only 2 cases received antiretroviral therapy.The misdiagnosis rate was 31.6%, and mortality rate was 21.1%.Conclusions AIDS coinfected with cryptococcus meningitis onsets hidden, with the clinical manifestation is atypical, and the misdiagnosis rate is higher.Early diagnosis and early usage of appropriate antifungal therapy/antiretroviral therapy can help to prevent the development of it.

Multiple sclerosis (MS) is a demyelinating autoimmune disease of the central nervous system. It mainly occurs in young and middle-aged people and has a high disability rate. The pathogenesis of MS has not been fully elucidated, with great genetic heterogeneity, diverse clinical phenotypes, lack of specific biomarkers, numerous and complicated research data, which bring great challenges to the diagnosis and treatment of MS. Artificial intelligence (AI), as the cutting-edge hotspot in the development of science and technology, is widely used in the medical field to assist in the diagnosis, treatment and prognosis of various diseases. Its infinite learning ability greatly promotes the management of MS. This article will review the application of AI in MS diagnosis and treatment.

Objective To investigate the role of SCF on neuronal apoptosis induced by diabetes and its possible mechanism.Methods Twenty-seven male C57 mice were randomly divided into control group,diabetes group,and diabe-tes plus stem cell factor(SCF)group.The diabetic mice were induced by streptozotocin.TUNEL staining was used to assess neuronal apoptosis and western blot were used to detect the protein level of BCL-2,BAX,CASPASE 3 and P-ERK/ERK.Results Compared with the controls,the number of apoptotic neuron death and the protein levels of active CASPASE 3 were significantly increased in the cortex of diabetic mice.Treatment with SCF significantly reduced apoptotic neuron death and attenuated the increased in protein levels of active CASPASE 3 in the cortex of diabetic mice.The levels of BCL-2 and BAX were significantly increased in the diabetic animals compared to the controls.Treatment with SCF could significantly attenuated the increase in the expression of BAX but could not affect the level of BCL-2 in the cortex of diabetic mice.P-ERK was significantly decreased in the diabete group but not in dibete plus SCF group.Conclusions SCF can protect a-gainst diabete-induced apoptotic neuron death through increasing the phosphorylation of ERK and influencing the expression of BCL-2/BAX.

DEC1 is a basic helix-loop-helix(bHLH)transcription factor involved in the regulation of cell prolif-eration;cell differentiation;lymphocytes maturation;circadian rhythms;immune response and response to hypoxia.Recent studies have revealed that the expression of DEC1 is abnormally high in various tumor tissues and cells.In addition;the expression of DEC1 in the tumor tissue is related to the malignancy of various cancer types.This paper;therefore;focuses on the relationship between DEC1 and proliferation;apoptosis;cellular senes-cence;invasion and metastasis of tumor cells;in order to elaborate the role of DEC1 in the pathogenesis and pro-gression of tumor.

Objective Observation of 2 diabetic patients with insulin homocysteine(Hcy) levels before and after treatment ,to explore new diagnosis of diabetes patients with blood glucose control for the effect Hcy .Methods Extracted from 128 cases accord with standard of newly diagnosed type 2 diabetic patients ,randomly divided into control group and strengthen group ,64 cases each . Give control group oral sulfonylureas .To observe group use insulin intensive therapy .In the three months after treatment compared two groups before and after treatment of FBG ,HbA1c indicators related to change .Results All patients after treatment ,Hcy , FBG ,PBG ,HbA1c have decreased ,and Hcy in strengthen group decreased more obviously than in control group ,the difference was statistically significant level changes(P<0 .05) .Conclusion Insulin intensive therapy can be effective in the treatment of newly di‐agnosed type 2 diabetes .

Military hygiene is one of compulsory courses for undergraduates majored in clinical medicine in military colleges. Aiming at the disconnection between theoretical and practical ability in the traditional teaching process and combining with professional features of military hygiene, teaching reform should give priority to curriculum standards, human resource construction, teaching content and method, teaching quality assessment and feedback system. Meanwhile, teaching idea of‘learning to use’ should be strengthened and teaching scheme in line with the actual needs of military health service security work should be put forward in order to provide new ideas for training military health personnel.