To explore the effect of Ruiqi pill on neuronal calcium level of cerebral cortex in brain of rat embryos.Methods: The cerebral cortex of rat embryos pregnanted for 16-17 days were dissected.The neural cells were divided into Ruiqi pill group and control group randomly after 4-5 days in vitro culture.Effect of Ruiqi pill on neuronal calcium level of primary culture of cortical neurons in rat embryos was measured by laser scanning confocal microscopy.Results:Ruiqi pill could reduce neuronal calcium concentration,no change was found in the control group.Conclusion: Ruiqi pill reduced neuronal calcium concentration,which might be one of the mechanisms of Ruiqi pill in improving memory function.

OBJECTIVETo explore the effect of Ruiqi tablet on mitochondrion activities in the cerebral cortex neurons of fetal mice.

METHODThe cerebral cortex of fetal Wistar rats after 16- 17 gestation days were collected and randomized into Ruiqi tablet group and blank control group after 4-5 days' culture. Laser scanning confocal microscope was adopted to determine the changes in the mitochondrion activities of the primary cultured cerebral cortex neurons of fetal rats after addition of Ruiqi tablet solution.

RESULTRuiqi tablet can increase the mitochondrion activities of the cerebral cortex neurons. No significant change in the mitochondrion activities of the cerebral cortex neurons was found in the blank control group.

CONCLUSIONRuiqi tablet can increase the mitochondrion activities of the neurons and it has certain application prospects in treatment of some neurodegenerative diseases such as Alzheimer's disease, vascular dementia, Parkinson's disease and so on.

Animals ; Cerebral Cortex ; cytology ; drug effects ; physiopathology ; Drugs, Chinese Herbal ; pharmacology ; Female ; Fetus ; chemistry ; drug effects ; physiopathology ; Humans ; Microscopy, Confocal ; Mitochondria ; drug effects ; physiology ; Models, Animal ; Neurons ; drug effects ; physiology ; Random Allocation ; Rats ; Rats, Wistar ; Tablets

Objective To explore the feasibility of reducing the tube voltage to 80 kV,while ensuring the CT image quality for the diagnosis when the patients with high BMI (26 kg/m2≤BMI≤30 kg/m2)undergo coronary CTA examination,using low concentration isotonic contrast medium (270 mg I/mL)and iterative reconstruction techniques (SAFIRE-3).Methods Sixty patients with high BMI who underwent coronary CTA were randomly divided into group A and group B.Group A was set as"double low"group (n=30,tube voltage=80 kV),using SAFIRE-3 technology and 270 mg I/mL contrast agent.Group B was set as conventional group (n=30,tube voltage=120 kV),using filtered back projection (FBP)technology and 350 mg I/mL contrast agent.Adaptive cardio-sequence prospective ECG-gated technology (CorAdSeq)was applied in both groups,and the collecting phase was 65%-75% R-R interval (heart rate <75 beats per minute)or 40%-50% R-R interval(heart rate &ge;75 beats per minute).Subjective and objective assessment methods were applied to evaluate the quality of images.The image quality and radiation dose of"double low"group was analyzed with conventional group as the control group.SPSS 1 9.0 software was applied for statistical analysis.The independent samples t-test was used to evaluate the differences among different measurements data.P<0.05 was considered statistically significant.Results The EDssde of group A [(1.124±0.314)mGy·cm]was significantly lower than that of group B [(3.542±0.797)mGy·cm],and the difference was statistically significant(P<0.001).The difference in noise between group A and B was statistically significant(P<0.05). The SNR and CNR in the group A were not lower than those in the group B and even the CNR in the partial coronary segment of the group A was higher than that in the group B,exhibiting statistically significant difference(P<0.05).Image quality index in the group A was higher than that in group B,and the difference was statistically significant (P<0.05).Conclusion Compared with conventional coronary CTA examination,"double low"coronary CTA examination in patients with high BMI (26 kg/m2≤BMI≤30 kg/m2)can acquire nice CT images suitable for clinical diagnosis and can significantly reduce the radiation dose and the amount of contrast agent.