1.Effect of mitofusin 2 gene with protein kinase A phosphorylation site deletion on the proliferation of vascular smooth muscle cells
Wei ZHOU ; Wenjin CAO ; Lili CHEN ; Xiaomei GUO ; Guanghui CHEN
Chinese Journal of Tissue Engineering Research 2010;14(7):1322-1326
BACKGROUND: The mitofusin 2 (Mfn2) may affects vascular smooth muscle cell Ras protein and suppress cellular proliferation through inhibition of extracellular signal-regulated protein kinase signaling pathway, which plays an important role in pathogenesis of vascular disorders such as hypertension, atherosclerosis and post-angioplasty restenosis. Mfn-2 gene amino acid sequence of the first 442 serine serves as protein kinase A (PKA) phosphorylation site, which is closely related to its phosphorylation status and may be involved in its functional regulation.OBJECTIVE: To investigate the effect of Mfn2 gene with PKA phosphorylation site deletion[Mfn2-PKA (△)] on inhibiting the proliferation of vascular smooth muscle cells and related signaling pathway.METHODS: Vascular smooth muscle cells of rats infected by recombinational adenovirus carrying green fluorescent protein,Mfn2 gane and Mfn2-PKA (△), were subcultured for 3-10 passages and randomly divided into 4 groups: ① Control group without intervention. ② Control group infected with adenovirus carrying green fluorescent protein. ③ Experiment group infected with adenovirus carrying Mfn-2 gene.④ Experiment group infected with adenovirus carrying Mfn2-PKA (△). Laser confocal microscopy was used to observe the locations of Mfn2 gene with and without PKA in cells. The expressions of extracallular signal-regulated protein kinase, Mfn2 gone and Mfn2-PKA (△) were determined by Western blot analysis. The growth curve of the vascular smooth muscle cells was explored by MTT.RESULTS AND CONCLUSION: The Mfn-2 and Mfn2-PKA (△) both expressed protein-specific bands in vascular smooth muscle cells. Two kinds of gone expression products were mainly located at the out membrane of mitochondria. Compared with the control group and adenovirus carrying green fluorescent protein group, the absorbance values at 3, 4, 5, 6 days were significantly reduced in adenovirus carrying Mfn2 group (P < 0.01), and no obvious changes were observed in adenovirus carrying Mfn2-PKA (△) group. Compared with the control group and adenovirus carrying green fluorescent protein group, the extracellular signal-regulated protein kinase expression was significantly reduced in adenovirus carrying Mfn2 group (P < 0.01), and no obvious changes were observed in adenovirus carrying Mfn2-PKA (△) group. Mfn2-PKA (△) located at the out membrane of mitochondria, has no effect on suppressing the proliferation of vascular smooth muscle cells, and no inhibition effect on extracellular signal-regulated protein kinase signaling pathway.
2.High-frequency oscillatory ventilation in children with measles complicated with severe pneumonia and the acute respiratory distress syndrome
Wenjin GENG ; Lijing CAO ; Meixian XU ; Xiaodong WANG ; Ximin HUO
Chinese Pediatric Emergency Medicine 2015;22(12):844-847,851
Objective To explore the effectiveness and safety of high frequency oscillatory ventilation(HFOV) in children with measles complicated with severe pneumonia and the acute respiratory distress syndrome(ARDS).Methods A total of 63 children with measles complicated with severe pneumonia and the ARDS were divided into conventional mechanical ventilation(CMV) group and HFOV group.The PaO2/ FiO2,oxygenation index (OI),HR and mean arterial pressure (MAP) before treatment and 12 h,24 h,48 h after treatment were detected.The rate of air leak and the motality in two groups were compared.The efficacy and safety of HFOV treatment were evaluated in children with measles complicated with pneumonia and severe ARDS.Results In HFOV group,the PaO2/FiO2 ratio was elevated and OI was decreased significantly after 12 h and maintained for at least 48 h.Compared with CMV group,OI of HFOV group improved more significantly,and the difference was statistically significant.The ventilation time in HFOV group was shorten than that in CMV group[(7.97 ±3.06) d vs.(11.03 ±3.60) d],but there was no statistical difference between two groups (P > 0.05).The heart rate after treatment 48 h was gradually returned to normal,and there was no statistical difference between the two groups.There were no significant changes in the MAP of two groups after treatment.There were no significant differences in the incidence of air leak between the CMV group and the HFOV group(24.2% vs.16.7%).The mortality rate of CMV group and HFOV group was respectively 45.5 % and 33.3 %,and there was no statistically significant difference.Conclusion HFOV was effective in oxygenation and seems to be safe for pediatric patients with measles complicated with severe pneumonia and the ARDS.Also it didn't influence the occurrence of complications.It has no adverse influence on hemodynamic parameters.Early intervention of HFOV is safe and effective for the children with measles complicated with severe pneumonia and ARDS.
3.Detection of major allergen parvalbumin in fish
Qiufeng CAI ; Guangming LIU ; Ruifang WANG ; Xichang WANG ; Wenjin SU ; Minjie CAO
Chinese Journal of Immunology 2000;0(08):-
Objective:To develop an indirect enzyme-linked immunosorbent assay (ELISA) for semi-quantification of major allergen parvalbumin in fish.Methods:The soluble proteins were prepared from both white and dark muscles of seven species of freshwater fish and five species of marine fish.Tricine-SDS-PAGE and Western blot were performed to examine the protein patterns of fish muscle extracts.Natural parvalbumin being used to make calibration curve was purified from silver carp (Hypophthalmichthy molitrix) by ammonium sulphate fractionation,followed by ion exchange and gel filtration chromatography.The molecular mass of purified protein was estimated by Tricine-SDS-PAGE and identified by Western blot with anti-frog parvalbumin monoclonal antibody PARV-19.ELISA using PARV-19 was carried out to evaluate parvalbumin contents in white and dark muscles.Results:Tricine-SDS-PAGE revealed species-specific differences in proteins of heated extracts.Western blot confirmed that the major bands were showed in Tricine-SDS-PAGE with the molecular masses of 10-14 kD corresponded to parvalbumins recognized by PARV-19 and various numbers of isoforms of parvalbumin existed in different species of fish.There might be some differences in the parvalbumin contents and the epitope region was recognized by PARV-19 based on the differences in relative intensities of protein immunodetection.The ELISA showed that the contents of parvalbumin were 4 to 33 folds higher in the white muscle than in the dark muscle and varied greatly in different species of fish.Conclusion:These results validate that the dark muscle might be less allergenic than the white muscle due to the lower content of parvalbumins,and it is suggested that the commercial anti-parvalbumin antibody PARV-19 can be used to detect parvalbumins from the commercially important species of fish tested in this study and the method we develope succeeds to detect the major allergen in various species of fish.
4.Interleukin 10 knockout increases renal fibrosis of ischemia-reperfusion injury model mice
Wenjin SUN ; Xin WAN ; Wen CHEN ; Wenjuan HUANG ; Dong SUN ; Changchun CAO
Chinese Journal of Nephrology 2016;32(2):143-148
Objective To study the effect of interleukin (IL)-10 knockout (IL-10-/-) on renal repair after renal ischemia-reperfusion injury in mice.Methods Eighteen IL-10-/-mice (KO) aged 8-10 weeks and 18 C57BL/6 wild type mice (WT) aged 8-10 weeks were divided into control group (Sham) and renal ischemia-reperfusion injury (IRI) group.The renal tissue morphology change was observed by Hematoxylin and eosin (HE) staining and Masson staining.The expressions of IL-18, Ki67 and TGF-β1 were detected by immunohistochemistry.The expression of TGF-beta1 and IL-18 were detected by Western blotting.Results Compared with that in WT-IRI group, in KO-IRI group renal pathological damage was more severe, renal interstitial fibrosis was visible, Ki67 expression of renal tubular epithelial cells decreased distinctly (P<0.01), the expression of TGF-betal increased significantly (P<0.01).Conclusion Repair slows down significantly after kidney ischemia-reperfusion injury and fibrosis occurs gradually in IL-10-/-mice, eventually progressing to chronic kidney disease.
5.The significance of monitoring blood coagulation function in children with severe hand,foot and mouth disease
Xiaodong WANG ; Ximin HUO ; Meixian XU ; Yinrui ZHANG ; Wenjin GENG ; Lijing CAO ; Hui SUN ; Yanmei GUO ; June LI ; Lei KANG
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2014;(1):26-30
Objective To investigate the relationship between the coagulation system status and the pulmonary hemorrhage in children with severe hand,foot and mouth disease(HFMD)and approach the clinical significance of early detection of coagulation function. Methods By prospective case design method,89 cases with HFMD admitted to Department of Critical Care Medicine of Hebei Provincial Children Hospital from July 2010 to July 2012 were enrolled. The children were divided into severe group(46 cases)and critical group(43 cases)according to the severity of disease,and the children in critical group were subdivided into survivor group(26 cases)and non-survivor group (17 cases). Forty-four healthy children with the same age and in the same period were served as healthy control group. The blood of children was collected immediately after admission for determination of blood routine, prothrombin time(PT),thrombin time(TT),activated partial thrombin time(APTT),fibrinogen(Fg),and D-dimer (DD). Results There were no significant differences in PT,TT,APTT and Fg among severe group,critical group and health control group(all P>0.05). The blood platelets count(PLT)in severe group and critical group was significantly lower than that in health control group(×109/L:245±130,237±156 vs. 389±120),while the DD was significantly higher than that in healthy control group(mg/L:0.34±0.67,0.41±0.08 vs. 0.24±0.13),and the DD in critical group was obviously higher than that in severe group(all P<0.05). The mortality rate in critical group was 39.5%,and there were no significant differences in PT,APTT,Fg,TT and PLT between survivor group and non-survivor group(all P>0.05),but the DD in non-survivor group was significantly lower than that in survivor group(mg/L:0.60±0.09 vs. 0.12±0.09,P<0.05). Conclusions In children with severe or critical HFMD, the coagulation factor and blood platelet were in a state of mobilization,mild consumption state with the existence of fibrinolytic inhibition,but without systemic bleeding tendency,therefore it is in a compensatory stage of disseminated intravascular coagulation(DIC),not the mechanism of pulmonary hemorrhage. The monitor of DD has its clinical significance in evaluations of the disease situation and its prognosis.
6.Preliminary voxel-based morphometry analysis of grey matter abnormalities in early stage of bipolar disorder I
Wenjin ZOU ; Liping CAO ; Liqian CUI ; Xuan LI ; Guimao HUANG ; Chongpeng SUN ; Guohui LAO ; Jianhua CHEN ; Yufen OU ; Wenhao DENG ; Xinchun LI
Journal of Practical Radiology 2014;(7):1075-1079
Objective To estimate brain grey matter volume changes and location of abnormal brain regions cerebrum in early stage of bipolar disorder I (BD),in order to provided objective basis for diagnosing early stages BD.Methods 1 7 cases of BD with duration less than 2 years and 1 7 normal controls were recruited in this study.The volumetric difference of grey matter between two groups were analyzed by using voxel-based morphometry(VBM)software.Statistical threshold was voxel> 100,P <0.001 (uncor-rected).Results Compared to the normal controls,the grey matter volume of BD patients decreased in the left dorcial anterior cingu-late cortex(ACC),left insular,right sub-genu ACC,left superior temporal cortex,bilateral hippocampus-parahippocampus-amydala and left posterior lobe of cerebellum(P <0.001).Conclusion The grey matter volume of early stage BD patients is decreased,main-ly locating in the bilateral limbic system,the superior temporal gyrus and the cerebellar cortex,which probably is the morphological appearance of pathomechanism in early stages of BD.
7.Research progress of evaluation tools for sexual health care of nurses in oncology department
Jing XU ; Wenjin ZHANG ; Wenli LIU ; Zhiqin CAO ; Xingfeng CHEN ; Yao FU
Chinese Journal of Practical Nursing 2023;39(25):1996-2001
This paper introduces the concepts related to sexual health and sexual health care, summarizes the contents, application scope and limitations of sexual health care assessment tools for oncology nurses at home and abroad, analyzes the problems existing in the assessment tools and puts forward suggestions, aiming at providing theoretical reference for the localization development of sexual health care assessment tools and the development of sexual health care.
8.Comparative study of direct mechanical thrombectomy and bridging therapy for acute anterior circulation large-artery occlusive stroke
Haibing REN ; Huiqin LIU ; Sisi WANG ; Jianying ZHANG ; Wenjin YANG ; Xiaohui ZHAO ; Ke QING ; Guodong XIAO ; Yongjun CAO
Chinese Journal of Neuromedicine 2020;19(2):131-137
Objective To comparatively analyze the safety and efficacy of direct mechanical thrombectomy and bridging therapy for patients with acute anterior circulation large-artery occlusive stroke.Methods A total of 116 patients with acute anterior circulation large-artery occlusive stroke,admitted to our hospitals from October 2015 to March 2018,were chosen in our study;their clinical data were analyzed retrospectively.Among them,63 patients accepted direct mechanical thrombectomy and 53 accepted bridging therapy.The preoperative baseline data and the diagnoses and treatments of the two groups were analyzed;the degrees of modified thrombolysis in cerebral infarction (mTICI),incidences of hemorrhage transformation and symptomatic intracranial hemorrhage,and modified Rankin scale (mRS) scores and mortality rate 90 d after operation were compared between the two groups.Results The preoperative Alberta stroke program early CT scale (ASPECTS) and Glasgow Coma Scale (GCS) scores of the direct mechanical thrombectomy group were significantly lower than those of the bridge therapy group (P<0.05),and the time from onset to admission was significantly longer than that of the bridging therapy group (P<0.05).The incidence of postoperative hemorrhage transformation in the direct mechanical thrombectomy group was significantly higher than that in the bridging therapy group (34.9% vs.17.0%,P<0.05),but there were no significant differences in the effective recanalization rate (69.8% vs.79.3%),intracranial symptomatic hemorrhage rate (15.9% vs.7.6%),favorable outcome rate (28.6% vs.35.9%) and mortality (22.2% vs.17.0%) between the two groups (P>0.05).Conclusion The clinical efficacy and safety of direct mechanical thrombectomy and bridging therapy for patients with acute anterior circulation large-artery occlusive stroke are similar.
9.Simultaneous Determination of 8 Non-anthraquinone Constituents in Rheum palmatum by HPLC
Wenjin LU ; Zhihua DOU ; Rui CAO ; Lili NI ; Ying DAI
China Pharmacy 2019;30(14):1975-1980
OBJECTIVE: To establish a method for simultaneous determination of 8 non-anthraquinone constituents in Rheum palmatum. METHODS: HPLC method was adopted. The determination was performed on Symmetry C18 column with mobile phase consisted of methanol-0.1% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ and detection wavelength was 280 nm. Sample size was 30 μL. RESULTS: The linear range of gallic acid, catechin, epicatechin, resveratrol 4′-O-glucopyranoside, epicatechin gallate, resveratrol 4′-O-β-D-(6″-O-galloyl)-glucopyranoside, sennoside A, 4′-hydroxyphenyl-2-butanone-4′-O-β-D-(2″-O-galloyl-6″-O-p-hydroxy cinnamyl)-glucopyranoside were 6.16-2 464 ng(r=0.999 9), 37.4-14 960 ng(r=0.999 9), 7.635-3 054 ng(r=0.999 7), 7.63-3 052 ng(r=0.999 9), 8.32-3 328 ng(r=0.999 9), 11.5-4 600 ng(r=0.999 9), 16.08-6 432 ng(r=0.999 9), 29.3-11 720 ng(r=0.999 9), respectively. The limits of quantitation were 3.48, 4.30, 6.40, 4.40, 3.39, 2.87, 8.40 and 4.95 ng, respectively. The limits of detection were 2.32, 2.58, 2.40, 2.64, 2.26, 1.23, 4.20, 2.97 ng, respectively. RSDs of precision, stability and reproducibility tests were all lower than 5%. Recoveries were 94.32%- 100.54%(RSD=2.78%,n=6), 91.15%-99.36%(RSD=3.72%,n=6), 92.16%-98.04%(RSD=2.39%,n=6), 93.41%-100.73%(RSD=3.17%,n=6), 93.89%-98.40%(RSD=1.99%,n=6), 92.61%-101.74%(RSD=3.71%,n=6), 92.66%-103.40%(RSD=3.76%,n=6), 95.45%-102.70%(RSD=3.06%,n=6), respectively. CONCLUSIONS: The established method is simple, accurate and specific, and can be used for the simultaneous determination of 8 non-anthraquinone constituents in R. palmatum.
10.Construction of the content index of "Introduction to Sexual Science" as an elective course for nursing master based on Delphi method
Wenjin ZHANG ; Zhiqin CAO ; Xingfeng CHEN ; Yao FU
Chinese Journal of Practical Nursing 2023;39(19):1492-1498
Objective:To combine sexology with nursing teaching organically, and construct the content index of the elective course Introduction to Sexology for nursing master, so as to provide reference for promoting the construction of new medical education in China.Methods:This study was a cross-sectional survey. The elective course content of Introduction to Sexual Science for Master of Nursing was preliminarily drawn up through literature analysis,by consulting the literature and materials. From June to October in 2021, by using Delphi method, 20 sexual science experts from 9 provinces and municipalities with relevant experience in master′s teaching were interviewed for two rounds. After statistical processing, the course content indicators were selected according to the importance assignment, coefficient of variation, full score rate and recognition rate of the indicators, and the course content indicators were determined.Results:The positive coefficients of experts in two rounds of correspondence were 83.33% and 100.00% respectively, the authoritative coefficients of experts were 0.818 and 0.815 respectively, and Kendall's harmonious coefficients were 0.137 and 0.403 respectively. The course content of "Introduction to Sexual Science" was composed of 11 first-level indicators and 46 second-level indicators. Its importance value was 4.15-5.00, the coefficient of variation was 0.000-0.136, the full score rate was 20.00%-100.00%, and the recognition rate was 95.00%-100.00%.Conclusion:The course content of "Introduction to Sexual Science" for nursing postgraduates constructed in this study takes the knowledge of sexual psychology and sociology as the core, pays attention to the cultivation of sexual values, combines theoretical knowledge of sexual medicine with clinical practice ability, and has high enthusiasm and authority of experts and good coordination of opinions. It is of positive significance to supplement the lack of sexual health courses for nursing postgraduates and cultivate nursing postgraduates with certain sexual health care literacy.