Acid a-naphthyl acetate esterase (ANAE), a cytoplasmic marker was used to identify the T lymphocytes and their subpopulations in peripheral blood of mice experimemally infected with T. spiralis. The results showed that the absolute number of lymphocytes and T lymphocytes increased in parallel on d, after infection, reachiug the peak on d14, and remaining above normal level on d60. The spotted granular ANAE positive cells (Helper T cells, Th) were decreasing and the scattered granular ANAE positive cells (suppressor T cells, Ts) were increasing, leading to a lowering of the Th/Ts ratio which was most marked on d14 and returned to normal on d60. The depres-sion of host immune function during T. spiralis infection might be related to the lowering of Th/Ts ratio.

Objective To explore the drugs combined with psychological therapy for lumbar disc herniation, summarize the clinical experience.Methods For 60 cases of lumbar disc herniation were treated with drug therapy, and classified as the control group, the other 60 patients in the control group on the basis of psychological intervention therapy, and classified as the observation group, two groups of patients were in our hospital from January 2016 to January 2017 were treated.Results No significant difference between the two groups of patients before treatment, the pain score, after grouping after the intervention in the observation group were obvious pain relief;compared two groups of patients with the emotional state of the visible, before treatment had no significant difference after The observation group after the intervention group were improved more significantly, shows significant difference(P<0.05).Conclusion The analysis showed that the drugs combined with psychological therapy for lumbar disc herniation, which can help patients to improve the clinical situation, adjust the patient's psychological anxiety and depression, improve the clinical effect of intervention, so it is worthy of reference.

The environmental pollution caused by organo tin attracted much concern,at the same time, the methodological study on determination of organo tin in environment made a desirable development, the chromatograph technique was used widely.A more detailed review on application of gas chromatograph and its coupled techniques in the environment organo tin compounds analysis was presented in this paper.

Objective:To investigate the effects of genistein (Gen) on apoptosis in human osteoblast. Methods:Human periosteum was maintained in DMEM medium containing 10% newborn calf serum through four passages to isolate and proliferate osteoblast. Then, cells were maintained in phenol red-free DMEM supplemented with 5% charcoal-stripped FCS for 4 d before addition of treatments. Flow cytometer and DNA fragment were used to explore apoptosis in osteoblast, and immunohistochemistry was used to study protein expression of Bax, Bcl-2 and caspase-3. Results:Estrogen depletion could induce apoptosis in human osteoblast. With the similarity of 10-8 mol/L E2, at the conentrations of 10-6 mol/L and 10-5 mol/L, Gen could inhibit apoptosis induced by estrogen depletion. Compared with vehicle control, Gen significantly decreased protein expression of Bax and caspase-3 and increased protein expression of Bcl-2. Conclusion:Genistein could inhibit apoptosis induced by estrogen depletion in human osteoblast.

Objective: To explore the correlation regarding the prognostic influence between multiple lung lobe lesions and acquired pneumonia in hospitalized elderly patients by a Meta-analysis. Methods: We collected all studies which investigated the correlation regarding the prognostic effect between multiple lung lobe lesions and acquired pneumonia by searching China National Knowledge Infrastructure, Wanfang Database, Chinese Science and Technology Periodical Database, Chinese Biological Medical Literature Database, PubMed, and EMBase in accordance with the inclusion and exclusion criteria. hTe retrieval limit time of searches was from databases establishment to July 2014. hTe Meta-analysis was performed by using RevMan5.2 sotfware. We calculated the odds ratio (OR) and 95% conifdence interval (95% CI) by using heterogeneous tests.Publication bias was assessed by Egger’s test and funnel plot, and the sensitivity was analyzed. Results: Ten studies involving 1 836 patients were ifnally included, with 487 cases (the dead group) and 1 349 controls (the survival group). The Meta-analysis demonstrated that multiple lung lobe lesions was highly correlated with the prognosis for the aged acquired pneumonia (OR=3.22, 95% CI 1.84 to 5.63). Conclusion: Multiple lung lobe lesions increase the risk of death in the prognosis of the aged patients with acquired pneumonia.

Objective To analysis influence of perioperative function rehabilitation training combined with drug treatment on uri-nary incontinence recovery after laparoscopic radical prostatectomy .Methods The clinical data of 31 cases of incontinence after lap-aroscopic radical prostatectomy were retrospectively analyzed .Early functional rehabilitation training ,drugs and mental guidance were applied to the patients .Results 31 out of 91 cases who receiving the laparoscopic radical prostatectomy immediately suffered from urinary incontinence when removing catheter .9 cases rehabilitated after 7 days ,12 cases rehabilitated after 1 months ,and 29 cases rehabilitated after 6 months .Conclusion Urinary incontinence occurs more in the early laparoscopic radical surgery ,perioper-ative function rehabilitation training combined with drug treatment could effectively improve the function of patients with urinary continence .

Objective To explore the changes of etiological factors,age of onset,misdiagnosis rates,length of stay and prognosis in central nervous system (CNS) infections between the near 2 decades.Methods A retrospective analysis was conducted between hospitalized patients with CNS infections in affiliated Yijishan Hospital of Wannan Medical College from January 1993 to December 2002 (group A,n =346) and from January 2003 to December 2012 (group B,n =412).The proportions of bacterial infections,Mycobacterium tuberculosis infections,Cryptococcus infections and viral infections between the 2 groups were comparable.Age,gender,underlying diseases and prehospitalization applications of immunosuppressant in two groups were analyzed.The data of prehospitalization misdiagnosis rates,length of stay and prognosis of the two groups were also compared.Measurement data were analyzed by variance analysis or t test,and enumeration data were analyzed by x2 test.Results The proportions of bacterial infections,Mycobacterium tuberculosis infections,Cryptococcus infections and viral infections in two groups were 24.3％ vs 20.1％,29.5 ％ vs 35.7 ％,6.6％ vs 11.4％ and 39.6％ vs 32.8％,respectively (x2 =10.61,P＜0.05).Compared with group A,patients in group B were older [(38.8±8.9) years vs (43.8±11.4) years,t=6.73,P＜0.05],with greater numbers of underlying diseases (1.21 ± 0.34 vs 1.72 ± 0.41,t=18.41,P＜ 0.05) and longer pre-use of immunosuppressants [(7.76 ± 3.58) d vs (12.43 ± 5.96) d,t =12.77,P＜ 0.05].There was no significant difference in sex distributions between the two groups (x2 =0.97,P＞0.05).In group A,the misdiagnosis rates for bacterial infections,Mycobacterium tuberculosis infections,Cryptococcus infections and viral infections were 9.5％,42.2％,69.6％ and 12.4％,respectively; average length of stay was (11.02±5.13) d,(19.18±8.34) d,(21.12±9.26) d and (8.24±3.17) d,respectively; and remission rates were 88.1％,60.8％,34.8％ and 80.3％,respectively.In group B,the misdiagnosis rates for bacterial infections,Mycobacterium tuberculosis infections,Cryptococcus infections and viral infections were 8.4％,29.3％,42.6％ and 11.1％,respectively; average length of staywas (10.13±4.25) d,(17.26±5.82) d,(23.05±7.97) d and (7.05±2.94) d,respectively; and remission rates were 90.4％,72.8％,61.7％ and 84.4％,respectively.The misdiagnosis rates and remission rates of Mycobacterium tuberculosis infections and Cryptococcus infections between group A and group B were of statistical significance (both P ＜ 0.05).Conclusions With the increase of underlying diseases and wide use of immunosuppressants,the causes of CNS infections are changing,among which Mycobacterium tuberculosis infections and Cryptococcus infections are increasing,with a tendency of misdiagnosis and poor prognosis.

Objective To establish a dot blot hybridization technique for rapid detection of staphylococci and methicillin-resistant staphylococci.Methods Three pairs of primers were designed according to nuc gene of staphylococcus aureus,mecA gene of methicillin-resistance,tuf gene of staphylococci.Specific DNA probes were synthesized by polymerase chain reaction and labeled with biotin.The bacterial DNA inoculated on nitrocellulose filter was hybridized with these probes.The sensitivity and specificity were detected.Results The DNA probes with 270bp,310bp and 370bp were amplified by the three pairs of primers respectively.The probes were specific.Among 50 clinical isolates of staphylococcus aureus tuf and nuc gene were all positive and mecA gene in 22 isolates were positive.Positive rate of tuf,nuc and mecA gene in 30 staphylococcus epidermidis were 100%,0 and 30% (9/30) respectively.No hybridization in other non-staphylococci occurred.The established method could detect as low as 1ng of bacterial DNA.Conclusion The dot blot hybridization is of high value in rapid,effective identification of methicillin-resistant staphylococci.

Objective To investigate the antitumor effects of phycoerythrin from Gracilaria lemaneiftheormis.Methods The coarse phycoerythrin(CPE) was ig administered to sarcoma180 mice,then the antitumor effect of CPE in vivo was evaluated by the inhibition rate,thymus and spleen indexes.The growth inhibitory effect of phycoerythrin(PE) on cell lines HeLa,EC109,and vascular endothelial in vitro was examined with MTT assay.Influences on HeLa cell cycle and apoptosis of PE were observed by flow cytometry and annexin v-fitc/PI fluorescence staining.Results CPE exhibited inhibitory effect against sarcoma180 growth in mice at the dosage of 100～300 mg?kg-1with a fair dose-effect relationship,and the inhibitory rate was 49.23% at the dosage of 300 mg? kg-1.CPE could also obviously increase the weight of thymus;PE could obviously inhibit the growth of HeLa cell in a dose-effect relationship,the inhibitory rate could reach more than 70%.It could prevent the transforming of HeLa cell cycle from G2/M to S phase to cause apoptosis.But PE couldn't inhibit the growth of EC109 cell,while some growth stimulating effects of PE on vascular endothelial cell were observed.Conclusion The PE from Gracilaria lemaneiftheormis could observably inhibit sarcoma180 growth in vivo.Effects of PE on different cells in vitro were differ,but showed potent inhibitory effect to HeLa cell.

Objective To compare the differences of epidemiology, clinical characteristics, laboratory results and prognosis between patients with severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) infection and those with hemorrhagic fever with renal syndrome (HFRS).Methods Medical records of 16 hospitalized cases with SFTSV infection and 28 hospitalized HFRS cases from January 2012 to June 2016 were reviewed in affiliated Yijishan Hospital of Wannan Medical College.In details, the comparative analysis of patients between the two groups were conducted in sex, age, occupation, onset season, contact history, underlying diseases, fever duration, oliguria, bleeding (including petechiae, ecchymoses, gum bleeding, bloody stool and hematuria), secondary infection, consciousness disturbance, dialysis treatment, length of hospital stay, laboratory results and prognosis.Continuous variables of normal, non-normal distribution data were compared using two-sample t test and rank sum test, respectively.Categorical variables were showed in rate and compared using chi-square test.Results The differences between the two groups in age (t=2.585), occupation (χ2=4.914), onset season (χ2=4.325) and contact history (χ2=9.617) were all statistically significant (all P<0.05).In SFTSV infection group, the mean fever duration was (8.81±3.17) d.There were 2 cases of oliguria, 10 cases of bleeding, 7 cases of secondary infection, 5 cases of consciousness disturbance.No patient received dialysis.The average length of hospital stay was (13.44±7.91) d.In HFRS group, the mean fever duration was (5.96±2.20) d.In addition, there were 24 cases of oliguria, 25 cases of bleeding, 9 cases of secondary infection, 3 cases of consciousness disorder.Twelve cases received dialysis treatment in this group.The average length of hospital stay was (18.04±15.75) d.Furthermore, there were significant differences between the two groups in fever duration (t=3.511), oliguria (χ2=22.578), bleeding (χ2=4.490) and dialysis (χ2=7.392) (all P<0.05).The significant differences were also found in white blood cell count, blood urea nitrogen, serum creatinine, albumin, amylase, lipase, creatine kinase, serum sodium, chloride, calcium, carbon dioxide combining power and blood glucose between the SFTSV infection group and HFRS group (all P<0.05).However, there was no significant difference in prognosis between the two groups (Z=1.574, P=0.115).Conclusions There are differences in epidemiological history, clinical manifestations and laboratory findings between the SFTSV infection group and HFRS group, which may help differential diagnosis and treatment of these two diseases.