Colorectal cancer is a malignancy with poor prognosis and high mortality and is caused by multiple factors. Colonoscopy,flexible sigmoidoscopy,guaiac-based fecal occult blood test,immunochemical fecal occult blood test,fecal DNA test,CT colonoscopy and serum carcinoembryonic antigen test are the methods frequently used for screening of colorectal cancer,but they all have certain limitations. Elevation of methylation of SEPT9 is associated with the pathogenesis of colorectal cancer,and detection of the level of methylation of SEPT9 in peripheral blood can be used for screening of colorectal cancer in susceptible population. This article reviewed the application of peripheral blood SEPT9 DNA methylation assay for screening of colorectal cancer.

Objective:To explore the time interval of using electric toothbrush after the adhesion of zinc polycarboxylate cement (ZPC) and PanaviaTM F(PF) respectively.Methods:The facial surfaces of 60 premolars were exposed and embedded in self-curing resin with custom made cylinder-shaped molds.Then the facial surfaces were cut with a diamond saw and polished with waterproof polishing papers to create standardized dentin surfaces.30 co-cr ally test-pieces and 30 zirconia ceramic test-pieces (4.0 mm in diameter and 2.0 mm in height) were adhered on 30 dentin surfaces respectively with ZPC and PF.The samples of each kind were divided into 5 groups (n =6) and brushed with an electronic toothbrush for 30 seconds immedietly (group 1),12 h (group 2),24 h (group 3)and 48 h(group 4) after adhesion,respectively.The samples in group 5 without brush were the controls.Shear bond strength of the samples was measured and the fracture patterns were observed under steriomicroscope.Results:In the ZPC adhered samples the bond strength of group 1,2,3 was lower than that of group 4 and 5 (P ＜ 0.05),group 4 vs group 5,P ＞ 0.05.In the PF adhered simples the bond strenth of group 1 and 2 was lower than that of group 3,4 and 5 (P ＜ 0.05),group 3 or 4 vs group 5,P ＞ 0.05.The fracture patterns were mostly cement cohesive failure.Conclusion:The time intervals of using electric toothbrush after the adhesion of ZPC and PF are 48 h and 24 h respectively

Objective To investigate the effect of xuebijing injection applied after abdominal operation on the inflammatory reaction,infection rate,length of stay and hepatorenal function. Methods Sixty patients received abdominal operations were randomly divided into therapeutic group (30 cases) and control group (30 cases). The inflammatory reaction indexes (temperature,heart rate,leucocyte) and hepatorenal function indexes(aminopherase, total bilirubin, creatinine, blood urea nitrogen) were observed in both groups before operation and after operation 3,7 days respectively. The infection rate and length of stay were compared in two groups. Results Temperature and leucocyte post operative 3 days [temperature:(37.0 ± 0.2) ℃ vs. (37.9 ± 0.4) ℃ ;leucocyte: (8.8 ± 1.1 ) × 109/L vs. ( 10.3 ± 1.7) × 109/L, P＜ 0.05] and length of stay in therapeutic group was obviously better than that in control group[ (8.7 ± 1.9 ) d vs. ( 10.9±1.6) d, P ＜ 0.05 ]. The hepatorenal function indexes and infection rate in both groups had no significant difference(P ＞ 0.05). Conclusion Xuebijing injection could significantly palliate the inflammatory reaction after abdominal operation, shorten the length of stay and has no hepatorenal toxicity in short term.

Objective To evaluate the clinical effect of light cure Dyractflow (LCDF) in pit and fissure enamel-plasty sealant technique (EST). Methods A total of 400 first permanent molars in 200 patients aged from 6 to 10 years were treated with LCDF in occlusal pit and fissure enamel-plasty sealant technique. The opposite permanent teeth treated with LCDF in occlusal pit and fissure conventional sealant technique (CST) were employed as the controls. The patients were followed up for 3 years. Results Follow-up for 3 years showed that there was no significant difference in preservation rate between the EST and the CST groups in the first year, but in 2-3 years, there was significant difference in preservation rate (P

Objective To analyze the risk factors of root caries.Methods With randomly stratified cluster sampling method,we selected 600 elderly in Tangshan city and performed oral examination.Data was analyzed by Logistic regression analysis.Results Gingival recession,age,oral calculus index,false tooth restoration were the risk factors of root caries.The vertical brushing tooth method and using fluoride toothpaste were the protective factors.Conclusion Gingival recession,age,oral calculus index(CI-S),false tooth restoration were the major risk factors of root caries.

Objective: To explore the therapeutic effect of dexamethasone on proliferative retinopathy in different stages and its interaction with the vascular endothelial growth factor(VEGF).Methods: Models of oxygen-induced retinopathy(OIR) were established.Twenty C57BL/6J mice pups(7 days after birth) were randomized into 4 groups: normal control,pure OIR,early-treatment and late-treatment.Dexamethasone was administered at the dose of 0.5 mg/kg?d-1by subcutaneous injection to the early-treatment group from postnatal day 7 and to the late-treatment group from day 12,both for 5 days.Common pathological sections were made from both eyeballs of the C57BL/6J mice and detected by immunohistochemistry to count the nuclei of proliferative retinal vessel cells and investigate the expression of VEGF in the retina.Results: The nuclei of proliferative retinal vessel cells and the expressions of VEGF significantly increased in the pure OIR group as compared with the normal control(P

Objective To study the antibacterial constituents of Senecio cannabifolius.Methods The antibacterial effect of all the extracts was tested in vitro with Staphylococcus aureus,Bacillus subtilis(Gram-positive bacilli),and Escherichia coli(Gram-negative bacillus).Chemical constituents were isolated by chromatography.Physicochemical characters and spectroscopic analyses were employed for their structural identification.Results Five compounds were isolated and their structures were elucidated by chemical and spectral methods as follows: methyl-5-hydroxy-2-pyridinecarboxylate(E-13),6-hydroxy-7,7a-dihydro-2(6H)-benzofuranone(E-14),2-(1,4-dihydroxycyclohexanyl)-acetic acid(E-16),3-hydroxycyclohexanecarboxylic acid(E-17),and 4-hydroxy benzaldehyde(B-14).Conclusion All of them are isolated from the plants of Senecio L.for the first time.

Objective To investigate the meridian touching on neonatal hyperbilirubinemia effect of adjuvant therapy.Methods 120 cases of neonatal hyperbilirubinemia were randomly divided into the observation group and the control group,60 cases in the control group were treated with modified simple touch of domestic law,on the basis of modified simple touch,the observation group increased meridian touching,2 times per day,once every 15 min.The nursing effect was compared between two groups.Results The observation group was significantly better than the control group in items such as daily intake of milk,sleep time,meconium drained time,jaundice.Conclusions On the basis of modified simple touch of domestic law,the addition of meridian touching can effectively reduce neonatal hyperbilirubinemia serum bilirubin levels,prevent high bilirubin encephalopathy,and promote the growth and development of newborns.

Objective To investigate the clinical manifestations and mortality related risk factors in patients with acute superior mesenteric artery embolism (ASMAE).Methods Clinical data of forty-three confirmed ASMAE patients in the PLA General Hospital from June 2002 to June 2012 were retrospectively analyzed.All patients were classified into the survival group (28 cases) and the death group (15 cases)according to the prognosis.The prognosis associated factors were further analyzed.Results The study group consisted of 31 men (72.1％)and 12 women (27.9％),with average age of (63 ± 11) years.The majority patients with ASMAE in our study had history of atherosclerotic diseases.The main clinical manifestationsincluded abdominal pain [100％ (43/43)],nausea and vomitting [55.8％ (24/43)],hematochezia [32.6％ (14/43)].Abdominal CT scan was performed in 74.4％ (32/43) patients with a high positive result of 96.9％ (31/32).Weight loss occurred more frequently in survival group than in death group [32.1％ (9/28) vs 6.7％ (1/15),P =0.001].Moreover,weight loss has been shown as a protective factor for ASMAE survival (OR =0.75,P =0.038) by logistic analysis.Compared with the death group,the incidence of either peritoneal irritation sign or ascites was significantly lower in survival group [respectively 7.1％(2/28) vs 66.7％ (10/15),14.3％ (4/28) vs 73.3％ (11/15),P ＜0.05],which were two independent risk factors of mortality(OR =8.51,P =0.014 ; OR =3.07,P =0.028).The incidence of main artery embolism of superior mesentery artery (SMA) in death group was higher than that in survival group [93.3％ (14/15) vs 60.7％ (17/28),P =0.023].Main artery embolism of SMA was also an independent mortality risk factor of ASMAE patients (OR =5.05,P =0.039).A total of 18 patients were treated with enterectomy.Intestine excision length was shorter in survival group than in death group [(82.8 ± 25.2) cm vs (141.0 ± 18.1)cm,P =0.017].The time from onset to operation in survival group was shorter than that in death group [(44.8 ±29.7) h vs (69.1 ±28.0) h,P =0.013].Conclusions Patients with ASMAE based on chronic ischemia have a relative good prognosis for survival.Peritoneal irritation sign,ascites and main artery embolism of SMA were independent risk factors for death in ASMAE.Intestine excision length and the interval from onset to operation may affect the mortality of ASMAE patients.

ObjectiveTo assess the differential diagnostic value of serum intestinal fatty acid binding protein (I-FABP)in distinguishing intestinal ischemia patients from acute abdomen patients.MethodsA total of 151 patients with acute abdomen and 17 healthy controls from the PLA General Hospital were enrolled from November,2009 to August,2011. Serum I-FABP levels were measured by ELISA.According to the ROC curve,the cut-off value,sensitivity,specificity,positive likelihood ratio (PLR),negative likelihood ratio ( NLR),positive predietive value (PPV) and negative predictive value (NPV) were calculated. ResultsOf the 151 acute abdomen patients,there were 24 intestinal ischemia patients and 127 without intestinal ischemia.Serum I-FABP level in intestinal ischemia group [( 109.67 ±48.82) μg/L]was significantly higher than those in patients without intestinal ischemia [(36.78 ± 11.25) μg/L]and healthy controls[(8.33 ±6.25) μg/L]( all P values ＜0.01 ).The serum I-FABP cut-off value for the diagnosis of intestinal ischemia was 87.52 μg/L.Serum I-FABP was efficient in terms of sensitivity (0.762),NPV(0.963),PLR(3.05) and NLR (0.24) in the diagnosis of intestinal ischemia.ConclusionI-FABP is potentially useful for discriminating intestinal ischemia from acute abdomen.