Objective To provide the convenient and efficient screening program for the early detection and diagnosis of peptic ulcer by evaluatingthe clinical value of serum anti HP -IgG detection in the diagnosis of peptic ul-cer in health examination .Methods 116 participants underwent serum anti HP -IgG detection and gastroscopy ex-amination, taking the pathological biopsy results of gastroscopy examination as the gold standard .Their consistency and the diagnostic value of serum anti HP -IgG detection were compared by the Kappa consistency test .Question-naires were filled by participants after the examinations to evaluate the comfort level and conveniences during the ex -aminations as well as their overall satisfaction , and to evaluate the value of HP -IgG detection .Results 87 partici-pants were positive in serum anti HP -IgG detection , accounting for 75%, while 29 participants were negative , ac-counting for 25%.According to the results of gastroscopy examination , 29 participants were diagnosed with duodenal ulcer (25%), and 25 participants with gastric ulcer (21.55%), and 34 participants with hybrid ulcer (29.31%), and 28 participants without peptic ulcer (24.14%).Based on the results of serum anti HP -IgG detection and gas-troscopy examination, Kappa=0.744 (p=0.073) was found by the Kappa consistency test , and the consistency was good.After the calculation , the sensitivity ( the true positive rate ) was 94.25%, and the specificity ( the true negative rate) was 79.31%.The anxiety, comfort level, conveniences and patients'satisfaction of anti HP-IgG group were bet-ter than those of control group (p<0.05).Conclusion Serum anti HP-IgG detection is worthy of the clinical promo-tion as a screening program for the early detection and early diagnosis of peptic ulcer , because it boasts of high sensitivi-ty, a good consistency in gastroscopy examination ,less anxiety , convenience and satisfaction for patients .

Objective To develop a method for localizing a detecting capsule in the gastrointestinal tract.Methods A three-axis magnetic sensor was sealed in the detecting capsule.Three flat coils were fixed on patients' back.The coils were excited one by one to generate electromagnetic fields.The position and the orientation of the capsule could be determined by telemetering the signals from the magnetic sensor incorporated in the capsule.Results The telemetric localization system worked successfully.A localization model describing the relationship between the magnetic field strength and the position and orientation of the capsule,was correctly established basing on the principle of magnetic dipoles,and neural network algorithm was employed to solve a non-linear equation set.Conclusion The results showed that the localization method is feasible and the precision is higher than existing methods.The localization device can perform consecutive tracking and can be made into a portable type.After putting into practice,the device can also be used in food selection for astronauts by monitoring their gastrointestinal parameters in space.

Objective To investigate the mechanism of Sut melanocytes growth inhibition in response to xCT -deficiency. Methods TTotal proteins were extracted from xCT-deficient Sut melanocytes and wild melanocytes, respectively, and were separated by 2-dimensional electrophoresis. Altered expression profile of proteins of Sut melanocytes was analyzed by PDQuest software and compared with that of wild melanocytes.Proteins with significant change were chosen to be identified by mass spectrometry and database query.Results Twenty proteins in Sut melanocytes altered significantly compared with wild melanocytes. Ten of the proteins were up-regulated, while the other tens were down-regulated. Four proteins from both up-regulated and down-regulated were identified respectively: up-regulated proteins were Tubulin alpha-1b, S100-A6,Nucleoside, S-formylglutathione, and down-regulated proteins were Calumenin,NDRG1 ,DPYSL2, 14kDa unknown protein. Conclusion The identification of the xCT-deficiency related proteins may provide supporting evidence for the mechanism research of Sut melanocytes' growth inhibition caused by xCT-deficiency.

The enzyme activity of α－Acetolactate Decaroboxylases (ALDC)from different microbes was studied, the results demonstrated that it was quite different among them. There were diversities of their enzyme reaction velocities. It was clear that the enzyme activity was affected by the pH of the enzyme reaction system, for example, the optimum pH of ALDC from Lactococcus lactis was 6.6, while for Aerobacter Aerogenes it was 5.8. Addition leucine,valine and isoleucine into enzyme reaction system obviously affected the enzyme activity of ALDC from different microbes.

Objective To investigate the value of detecting hepatitis B virus (HBV) YMDD variants by matrix-assisted laser de-sorption time of flight mass spectrometry (MALDI-TOF MS). Methods The assay is based on polymerase chain reaction (PCR) amplifica-tion and mass measurement of oligonucleotides containing sites of mutation of the YMDD motif. Result The MALDI-TOF MS-based genoty-ping assay was sufficiently sensitive to detect as few as 100 copies of HBV genome per milliliter of serum, and this method had superior spe-cificity for determining mixtures of wild-type and variant viruses. When sera of 40 patients were analyzed, the MALDI-TOF MS-based assay correctly identified known viral variants and additional viral quasi-species not detected by previous methods, as well as their'relative abun-dance. Conclusion The sensitivity, specificity and amenability to high-throughput analysis make MALDI-TOF MS-based assay suitable for mass screening of HBV infected patients who are receiving lamivudine.

Objective To evaluate the accuracy of different methods in detecting free amino acids in plasma.Method 40 blood samples from healthy volunteers were analyzed by an automatic amino acid analyzer ( Li+system) and the results compared with previous reports using other analyzers.Results The results ob-tained by this analyzer for major amino acids [asparagine (Asn), glutamic acid (Glu), glutamine (Gln), valine (Val), tyrosine (Tyr), phenylalanine (Phe), ornithine (Orn), arginine (Arg)] were similar with those previously reported using other amino acid analyzers ( all P>0.05 ) and liquid chromatography tandem-mass spectrometry (LC-MS/MS) (all P>0.05).Except for Glu and Tyr, the results for major amino acids showed large deviation compared to the results from high performance liquid chromatography ( HPLC) ( all P<0.05).Conclusion The amino acid analyzer (Li+) and LC-MS/MS (iTRAQ kit) could accurately detect free amino acids in plasma.

Objective To analyze the plasma amino acid levels and evaluate the free amino acids value of normal healthy volun-teers in plasma by establishing physiological fluid method.Methods ①Establishment of methodology:including repeatabili-ty of blood sample,recovery rate,linearity,and stability;②The free amino acid values in plasma in 40 normal healthy volun-teers were analyzed.Results With the standard solution of different concentrations (2,4 and 8 nmol)analyzed and linear re-gression of amino acid calculated,found that R2 of all the amino acids were within the range from 1 to 0.9 9 9.The repeatabili-ty with in batch and between batches of different amino acid in blood samples were respectively 0.30~2.90 (rsd)and 0.40~5.16(rsd);recovery rates were 93.1%~107.4%.Then evaluated the normal values of plasma amino acid in 40 healthy volunteers (male vs female:1 vs 1 ),and compared the results with that measured by other instruments.Conclusion With lithium citrate buffer system to analyze the free amino acid in blood samples,it displayed good reproducibility,recovery rate, linearity and stability.Comparison of different instruments measuring the free amino acids in plasma of normal healthy vol-unteers showed good agreement,confirming that the method of physiological fluid analysis is reliable.

Objective To differential the biologic characteristics and multiple differentiation potential of mesenchymal stem cells in nucleus pulposus in (NP-MSC) scoliosis patient and patient with degenerative interverthral disc.Methods The human nucleus pulposus-mesenchymal stem cells were isolated and cultured with enzyme digestion from 2 patients of scoliosis and 2 patient with degenerative intervertbral disc separately.Cellular proliferation was detected with MTT assay and trypan blue.The immunophenotype expression of NP-MSC was detected by flow cytometry in scoliosis and degenerative group.The multiple differentiation ability of cells was assessed respectively using alizarin red dye,Oil red O dye and immunohistochemical staining.Results The primary cell morphology of scoliosis NP-MSC was the shape of spindle,while the degenerative NP-MSC was inhomogeneous.However,both of them became spindle shape after passages.The scoliosis NP-MSC was stronger than degenerative one in metabolic activity and proliferation.The percentage of positive antigen expression of CD44,CD105 and CD29 was 97％-100％ in scoliosis NP-MSC group,but 88.7％-97％ in the degenerative group.The expression of mature cell marker CD24 was negative in both groups.Furthermore,the MSC i.isolated from both groups differentiated along the osteogenic,chondrogenic but not adipogenic lineages.Conclusion The scoliosis and degenerative NP contains mesenchymal stem cells.Moreover the scoliosis NP-MSC had stronger ability of cell metabolic activity and proliferation.These cells have multiple differentiation potential with the exception of their adipogenic differentiation ability.

Objective To study the effect of operative modalities on the prognosis of gastric carcinoma patients suffering from various types of intra-abdominal metastasis. Methods In this study, 150 cases of gastric cancer with abdominal metastasis screened from data base, were divided by the location into peritoneal dissemination group (n=106), hepatic metastasis group (n=19) and both peritoneal dissemination and hepatic metastasis group (n=25). The surgical interventions include total resection, palliative resection and no resection. The clinicopathological parameters and prognosis were analyzed. Results The total tumor resection rate in peritoneal dissemination group (48.1%) and hepatic metastasis group (63.2%) was significantly higher than that in both peritoneal and hepatic metastasis group (20.0%), with the mean survival time (months) being 31.1, 12.8, 9.7 respectively (P<0.05). In peritoneal group, the mean survival (months) for tumor total resection, palliative and no resection subgroup was 46.3,1.7,4.8 respectively (P<0.05), whereas in hepatic metastasis group, the mean survival (months) was 17.2,4.0,5.4 respectively (P<0.05), in both peritoneal and hepatic metastasis group, the mean survival (months) was 11.2,8.9,5.0 respectively (P>0.05). Lymph nodes resection significantly prolonged survival in peritoneal dissemination group. Conclusions The prognosis of gastric cancer with peritoneal dissemination is better than with hepatic metastasis. Tumor total resection and lymph node dissection significantly improve the survival of patients suffering from peritoneal or hepatic dissemination.

Objective To assess value of contrast enhanced ultrasonography(CEUS)in diagnosis of extracranial carotid stenosis.Methods Baseline ultrasonography and CEUS were performed in 108 patients with extracranial carotid stenosis.The location and degree of stenosis were judged and compared with the results of digital subtraction angiography(DSA).Results Of 108 patients,DSA diagnosed 81 vessels in 62 cases of mildintermediate stenosis which consisted of 33 common carotid arteries(CCAs),43 internal carotid arteries(ICAs)and 5 external carotid arteries(ECAS),51 vessels in 39 cases Of severe stenosis(46 ICAs and 5 CCAs)and 7vessels in 7 cases of complete occlusion(7 ICAs).The surface of intima and the interface of media-adventitia were depicted clearly by CEUS.The consistent rates in diagnosis on mild-intermediate stenosis,severe stenosis and complete obstruction by baseline ultrasonography and CEUS were 76.5%and 9 1.4%(P＜0.05).60.8%and94.1%(P＜0.001),28.6%and 100%(P＜0.05)respectively when judged by DSA as the golden standard.CEUS and DSA were correlated significantly on calculating the stenosis rate(r=0.975,P＜0.001).Conclusions CEUS can assess the degree of extracranial carotid stenosis accurately and non-invasively which is superior to baseline ultrasonography and correlated significantly with DSA.