OBJECTIVE:To discuss the practice of voluntary principle on the community pharmacy so as to provide some good suggestions for the smooth implementation of the GPP.METHODS:The voluntary principle of GPP was analyzed from several aspects,including the purpose of GPP,the integrated pharmaceutical service level,justice,law and policy and etc.RE_ SULTS:The feasibility of the implementation of voluntary GPP principle in China is expected to be verified,meanwhile,the concerned law and policy should be established in no time and the knowledge of medicine administration should also be publi?cized widely among the public.

Objective To establish a method to determine lead in air. Methods The filter film used to collect the lead in air was digested with acid followed by adding mixed solution of potassium ferricyanide,oxalic acid and hydrochloric acid, then double ways atomic flurescence spectrophotometry was employed to determine lead. Results The optimal concentration of hydrochloric acid, potassium ferricyanide and oxalic acid were 0.12 mol/L, 8 g/L and 0.4 g/L respectively. The detection limit of lead was 1 ?g/L, the relative standard deviation was 2.5%. The recovery rates ranged from 90% to 95%, the linar range was 10-80 ?g/L. Conclusion This method was simple, rapid, accurate and sensitive. It was suitable for determining lead in air.

Objective To establish a GC method for the determination of menthol in Fufang Caoshanhu Hanpian. Method HP-FFAP elastic quartz capillary vessel column was used,FID detector,programmed temperature,injection and detector temperatures was 250 ℃. Result The linear range of menthol were 0.020～ 0.300 ?g,respeetively. The average recoveries was 99.36% (RSD=1.12%). Conclusion This method is simple and accurate,and can be used for the determination method of menthol in Fufang Caoshanhu Hanpian.

Objective To explore the association between the 5-HTR2A (-1438A/G)promoter region gene polymorphisms and unipolar depression and effcacy of antidepressant drugs in northwest Chinese Han population.Methods Polymerase chain reaction (PCR) was used to detect the distributive frequency of serotonin 2A receptor promoter region polymorphisms of 136 unipolar depression patients( patient group) and 160 normal people tween the patient group and the control group.The frequency of AG,GG genotypes; and G allele in patients was phisms of 5-HT2AR promoter region were correlated with SSRIs treatment response(F= 6.317, P= 0.013 ).There was significant difference of the effective power at the end of week 2 ( x2 = 5.878, P= 0.015 ).The effective power of AA, AG genotype was much higher than that of GG genotype and the effective power in the group with AA genotype was higher than that with AG genotype( each 87.8% ,57.6% ,53.7% ).Conclusion The frequency of GG genotype of 5-HTR2A( 1348A/G)may be associated with episode of unipolar depression in northwest Chinese Han population and A allele may be correlated with well response to paroxetine.

OBJECTIVE:To discuss the pharmaceutical care trustworthiness imperfection from the perspective of eco?nomics and to put forward some suggestions for the reduction of trustworthiness imperfection behavior in the pharmaceutical care.METHODS:The behavior of the pharmaceutical care trustworthiness imperfection was analyzed from the perspective of profits maximum,information dissymmetry,and trade cost etc.in the economics theory.RESULT&CONCLUSION:It is of positive meaning to reduce pharmaceutical care trustworthiness imperfection behavior by establishing and improving related laws and rules,popularizing the medicine knowledge,strengthening monitoring,reforming the salary mode of the pharmaceuti?cal technical personnel.

We have studied mainly the distribution of this ~3H-labelled schistosomicidal drug in the livers and brains of the albino mice. In livers the ~3H-labelled substances were localized in greater quantity in the liver cell trabecula than in the sinusoids, and were taken up by the Kupffer's cells too. But the most prominent condense localization of the ~3H-labelled substances were in the interlobular bile ducts indicating that the original agent and its metabolites were excreted through the bile ducts system. The nuclei of the liver cells contained a certain amount lof the ~3H-labelled substances, about 7 silver grains in each nucleus with a diameter of about 10.7? seen in 2? thick freezing sections. One of the metabolites of this schistosomicidal drug being a mutagenic agent, the appearance of ~3H-labelled substances in the cell nuclei attracts our great attentions. As for the genesis mechanism of jaundice after administration of this schistosomicidal drug, our opinion is that it might be chiefly the result of drug sensitizied allergy reaction in accordance with a lot of works in the clinics, immunological test, pharmacological principles and our present studies. In the brains, the ~3H-labelled substances distributed much more in the white substances than in the gray substances, with the exception of the substantia nigra which had the same density of number of silver grains as the white substances. With these findingsthe clinical symptoms of the nervous system and their recovery were reviewed.

Objective To explore the application effect of ICU checklist of medical cooperation on prevention of ventilator-associated pneumonia. Methods Totally 728 patients with mechanical ventilation from July 2015 to July 2016 in ICU were assigned to the experimental group with using of the checklist; and 685 patients with mechanical ventilation from June 2014 to June 2015 were set as the control group who did not use the checklist. The incidence of ventilator-associated pneumonia and medical cooperation were compared before and after the implementation of the checklist. Results The incidence of ventilator associated pneumonia were 6.72% (46/685) and 3.02% (22/728), the intervention strategy execution rates were 63.36% (434/685) and 84.89% (618/728) before and after the application of checklist, there was significant difference between two groups (χ2=10.51, 86.03, P<0.01). Medical cooperation (doctors and nurses) and satisfaction scored 18.53 ± 2.39, 21.91 ± 2.16, 30.93 ± 2.18 and 26.71 ± 2.21, 28.64 ± 2.55, 45.33 ± 1.74 before and after the application of checklist, there was significant difference between two groups (t=6.14, 14.25, 2.39, P<0.01 or 0.05). Conclusions The application of checklist can improve the medical cooperation on prevention of ventilator-associated pneumonia management, reduce the incidence of ventilator-associated pneumonia, improve medical cooperation, nursing satisfaction and the management efficiency, which is worthy of popularization and application.

目的探索康复治疗专业学生社区实习基地规范带教方法和教学管理模式。方法对43名康复治疗专业学生进行问卷调查。结果绝大多数学生对社区康复教学的必要性表示肯定。超过半数学生对教学模式、内容比较满意，但教学效果的满意度低于教学模式及内容。社区康复教学在培养学生服务态度、沟通能力、操作能力方面有优势，在培养学生创造性思维能力、分析归纳能力、激发兴趣性存在明显不足。结论需要将三级医院康复的技术力量与社区康复教学纵向整合，摸索社区康复教学模式，不断提高社区康复师资水平。

10.3969/j.issn.2095-4344.2013.26.007

Objective To investigate prospectively the diagnostic significance of ulnar/median nerve amplitude ratio in motor neuron disorders.Methods Patients referral to our department between May 2009 and February 2012,due to muscle weakness,inflexible,cramps and atrophy,were consecutively enrolled.Conventional nerve conduction studies of 4 extremities (using surface electrodes) and needle electromyography were performed in all patients with fixed examiner.The compound muscle action potentials (CMAP) were recorded from abductor digiti minimi (ADM) and abductor pollicis brevis (APB) with stimulation of ulnar and median nerve at wrist,respectively.Moreover,the ratio of CMAP amplitude between ADM and APB (ADM/APB) was calculated in the patients who met the diagnostic criteria for definite amyotrophic lateral sclerosis (ALS) and Hirayama disease (HD).The patients with Guillain-Barré syndrome (GBS) and other popyneuropathies (PN) were served as case-controls,and 34 healthy volunteers (aged (45.7 ± 16.3) years) as normal-controls.Results (1) There were 78 cases with ALS,25 HD,51 GBS and 140 other PN,with the mean age(years) of 54.7 ± 11.6,17.6 ± 2.2,41.3 ± 18.4 and 57.1 ± 14.3,respectively.(2) ADM/APB in the ALS subgroup was 2.28 ±2.87 (0.12-22.38),HD0.66±0.36 (0.05-1.34),GBS 1.42 ± 1.33 (0.25-9.85),other PN 1.36 ± 1.48 (0.08-14.44) and normal-controls 1.07 ± 0.28 (0.61-1.64,F =6.872,P =0.000),respectively.(3) The areas under receiver operator characteristic curve in patients with ALS was 0.830 (s-x =0.039) and HD 0.691 (sx =0.039,P =0.000) ; the diagnostic sensitivity and specificity for ALS patients were 36.7％ and 93.3％,respectively,with cutoff value of ADM/APB =2; and the diagnostic sensitivity and specificity for HD patients were 53.6％ and 89.0％,respectively,with cutoff value of ADM/APB =0.7.Conclusions The ulnar/median CMAP amplitude ratio increases in ALS,but decreases in HD,which may be served as a relatively specific electrophysiological index.ADM/APB amplitude ratio ＞ 2.0 is suggested to be a diagnostic parameter for ALS and ＜ 0.7 for HD.