OBJECTIVE:To establish a method for determination of the dissolution of cibenzoline succinate tablets. METHODS: The content of cibenzoline succinate was determined by HPLC and computed based on the peak area in the external reference method. The chromatographic column was Diamonsil C18. The mobile phase consisted of acetonitrile-phosphoric acid(40∶60). The detective wavelength was set at 230 nm. The dissolution was determined by paddle method. RESULTS: The linear range of cibenzoline succinate was 1～40 ?g?mL-1(r=0.999 9) and the average recovery rate was 97.70%(RSD=0.14%).The dissolution rates of 3 batches of samples at 15 min were all above 80%.CONCLUSION: The established method is simple,rapid and accurate,and it is applicable for the determination of the dissolution of cibenzoline succinate tablets.

Whole-body vibration (WBV) training is a kind of new technology on the practice of rehabilitation medicine and is used to improve muscle strength, mobility, balance, osteoporosis, sarcopenia, metabolic syndrome and osteoarthritis in aging adults. The definition, safety, effectiveness, and biomechanics of WBV training were reviewed in this paper.

Whole-body vibration therapy (WBVT), in which energy produced by a forced oscillation is transferred to the muscle and bone of an individual from a mechanical vibration platform, has been proposed as an alternative or adjunctive intervention for osteoporosis. In this review, the safety, effectiveness and the biomechanics of WBVT for the prevention and treatment of osteoporosis were discussed.

Virtual reality (VR) offers the possibility of creating an interactive, motivating environment in which training intensity and feedback can be manipulated to provide individualized treatments or motor rehabilitation under a range of stimulus conditions that are not easily controllable and quantifiable in the real world, especially in elderly. This paper reviewed the studies illustrating the application, development and effectiveness of VR on rehabilitation in the elderly, that suggested VR may be useful to augment rehabilitation of a range of disabilities in elderly patients. The prospect of VR is discussed as well.

Objective To study the 5 years contraceptive efficacy of levonorgestrel intrauterine system (LNG-IUS) and the ovarian functions in amenorrhea women. Methods LNG-IUS and TCu 380A IUD were randomly inserted in 2 groups of women, 50 each. Women were followed annually for 5 years. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E 2), and T levels in 14 women with amenorrhea, and endometrial biopsies were obtained in 9 cases. Results By the end of 5 years, there were 2 pregnancies in TCu 380A group, but none in LNG-IUS group; and 24 amenorrhea cases in LNG-IUS. The 5 year cumulative continuation rates were 24% for LNG-IUS and 78% for TCu 380A ( P

BACKGROUND:Previous studies show that the fracture resistant force of endodonticaly treated teeth can be improved by post. But this idea has been controversial in recent years. Many scholars believe that the root canal preparation might weaken dental root the post increases the risk of tooth fracture. OBJECTIVE: To evaluate the fracture resistance ability of severe wedge-shaped-defect premolar to oblique loading, which was restored with fiber reinforced composite posts by different therapy methods. METHODS:A total of 50 human maxilary premolars were randomly divided into five groups, with ten teeth in each group. They were given folowing treatments: Group A: 10 untreated premolars; Rest 40 premolars of Groups B, C, D and E were prepared 1/3 bucco-lingual distance for artificial severe wedge-shaped defects at the buccal cervix. Group B: untreated severe wedge-shaped defects premolars; Group C: severe wedge-shaped defects premolars were endodonticaly treated, remaining dentin over hang above the wedge shaped defect, LuxaPost posts reinforced in buccal canal and lingual canal, LuxaCore composite resin restored dentin defect; Group D: severe wedge-shaped defects premolars were endodonticaly treated, LuxaPost posts reinforced in buccal canal and lingual canal, LuxaCore composite resin restored dentin defect, and then covered with ful metal crown; Group E: severe wedge-shaped defects premolars were endodonticaly treated, removing dentin over hang above the wedge shaped defect, LuxaPost posts reinforced in buccal canal and lingual canal, restored with LuxaCore composite resin, and then covered with ful metal crown. Fracture resistance of each specimen was measured in each group. RESULTS AND CONCLUSION: The fracture strength of each group were Group A (1 002.69±147.62) N, Group B (439.28±66.34) N, Group C (958.30±101.23) N, Group D (1 207.09±143.48) N, and Group E (1 056.44±139.30) N. Group D had the highest fracture strength (P < 0.01), while Group B had the lowest fracture strength (P < 0.01). There were no significant difference among the fracture strength of Group A, Group C and Group E. Our findings indicated that the fracture resistance of the severe wedge-shaped defected premolar can be improved by fiber reinforced composite post and dentin above wedge shaped defect remained.

Carotid artery stenosis is one of the most important reasons for stroke. Carotid artery stenting possesses more advantages with less damage and pain for patients, which has becaome the most effective treatment for carotid artery stensis, and the use of review the issues related to te carotid artery stenting under cerebral protection. With the accomplishment of many successful large scale clinical trials carried out by multiple centers, it is believed that carotid artery stenting with cerebral protection plays important orle in the prevevtion and treatment of cerebral stroke initiated by carotid artery stenosis.

Objective To screen the stable high-producing strains carrying anti-nasopharyngeal carcinoma(NPC)plasmid pFY.Methods Competent E.coli JM109 was prepared by the CaCl2 method and transformed with anti-NPC plasmid pFY.The bacterial colonies obtained from the agar plate were screened for selecting the single colony conforming to the standards as the bac-terial strain and conducting the stability test.The plasmid content was detected by the plasmid extraction reagent kit.Anti-NPC plasmid pFY was transfected into nasopharynegal carcinoma cell line CNE-2.The influence of transfection reagent and the plasmid vector on the cell proliferation was detected by MTT.Results The DNA concentration of plasmids in the culture solution of bacte-rial strain obtained by screening was 30 mg/mL.The proportion of supercoiled DNA was 92%.The identification of electrophoresis and restriction enzymes showed that the plasmids harbored in the 50th progeny of this strain were same as those in the primary. Plasmid pFY had the evident inhibiting effect on the growth of CNE-3 cell line.Conclusion The stable high-producing strains of E. coli carrying anti-NPC plasmid pFY is successfully screened out,which lays the foundation for large-scale preparation of plasmid pFY for clinical utility.

Objective To evaluate the effects of mechanical ventilation with heliox-oxygen on acute lung injury (ALI) in rabbits.Methods Thirty healthy New Zealand male rabbits, weighing 2.2-2.4 kg, were randomly divided into 3 groups (n =10 each) using a random number table: control group (C group), ALI group and mechanical ventilation with helium-oxygen group (HO group).The right common carotid artery was cannulated for fluid administration and blood sampling in the rabbits anesthetized with urethane.In ALI and HO groups, 1.2 ml/kg hydrochloric acid (HC1) of pH value 1.0 was injected into the right bronchus, followed by 2 ml air injection, and 5 min later 0.8 ml/kg HCI of pH value 1.0 was injected into the left bronchus, followed by 2 ml air injection.In C group, the equal volume of normal saline was given as previously described.After ALI, the rabbits were mechanically ventilated with airoxygen (air 50％-oxygen 50％) in C and ALI groups, or with helium-oxygen (helium 50％-oxygen 50％)in HO group.Mechanical ventilation (tidal volume 8 ml/kg, respiratory rate 30 breaths/min, inspiratory/ expiratory ratio 1 : 1) lasted for 4 h in the three groups.At 30 min before and after HC1 injection (T0,1) and 1, 2, 3 and 4 h of ventilation (T2-T5) , arterial blood samples were collected to detect the levels of surfactant-protein (SP-A) in serum by enzyme-linked immunosorbent assay.The animals were sacrificed at T5, and lungs were removed for examination of the pathologic changes with light microscope.The wet to dry lung weight ratio (W/D ratio) was calculated.Results Compared with group C, the levels of SP-A in serum were significantly increased at T1-T5, and W/D ratio was increased in ALI and HO groups.Compared with group ALI, the levels of SP-A in serum were significantly decreased at T5, and W/D ratio was decreased in HO group.Conclusion Mechanical ventilation with heliox-oxygen can reduce ALI in rabbits.

Objective To explore the clinical competence of nursing master of professional degree and give some suggestions for their postgraduate education. Methods A total of 19 samples of nursing masters of professional degree, nursing managers, nursing experts and clinical teachers were selected by using purpose sampling method, and semi- structured interview was adopted and the results were finally described and classified. Results The competence of the masters of professional degree came from the following five aspects: professional quality, practical ability, research ability, education ability and professional development ability. Conclusions A good professional quality is the premise of qualified for clinical work, flexible ability of practice is the basis of clinical work, solid scientific research background and the education ability is the core competitiveness, multi- dimensional professional development ability is the key to self-improvement, nursing professional degree graduate student should enhance their own comprehensive quality from the above five aspects, to be better qualified for clinical nursing work.