Objective To investigate the effect of total flavonoids of Herba Epimedii (HEF) on the apoptosis of osteoblasts in the ovariectomized (OVX) rats. Methods Fifty-four female rats were allocated into 6 groups (9 rats in each group) ; OVX group, sham-operated group, 3 groups with OVX followed by three different dosages of HEF(40,80 and 160 mg· kg-1·d-1) and another group by nilestriol (0.1 mg· kg-1·d-1) for 12 weeks respectively. Bone mineral density (BMD) of whole body of rats was determined by DEXA. The serum level of estradiol was measured by radioimmunologic assay. Apoptotic ceils were examined using terminal dexynudeotidyl transferase-mediated dUTP nick end labeling (TUNEL) and observed by electron microscope. The protein expressions of transforming growth factor (TGF)-β1 ,fibroblast growth factor (FGF)-2 and Fas were tested withimmunohistochemical methods. Results Compared with the OVX group,BMD in the HEF treated (80 and 160 mg ·kg-1·d-1) groups were increased (P < 0.01), but the serum level of estradiol was not increased (P >0.05). Positive expression rate of apoptotie osteoblasts and osteocytes in the OVX group was significantly higher than that in sham-operated group after 12 weeks (P < 0. 01), and were decreased after HEF treatment (P < 0.01).Compared with the OVX group, Fas expression in the HEF treated groups was significantly decreased (P < 0.01), and expressions of TGF-β1 and FGF-2 were significantly increased, especially in 80 and 160 mg·kg-1·d-1 HEF groups (P < 0.01). Conclusion HEF has antiosteoporotic effect in ovariectomized rats. HEF can inhibit apoptosis of osteoblasts and osteocytes, which may thus contribute to its antiosteoporotic effect.

Objective To investigate the clinical distribution and drug-susceptibility characteristics of yeast speeies in tumor patients with invasive yeast infections in order to provide the gist for clinical therapy.Methods The medical records of 70 tumor patients with invasive yeast infections from January 2006 to December 2008 were collected.retrospectively.The relationship between different tumor types and invasive yeast infections was analyzed.The clinical distribution and drug-susceptibility characteristics of 70 isolates of yeast were investigated.Results The sites of invasive yeast infections were abdominal(32 cases),blood(18 cases),biliary(9 cases),central nervous system(6 cases)and chest infection(5 cases).respectively.The most common species causing the above infections was Candida albicans(34 isolates,48.6%).The mortality of invasive yeast infection was 15.7%.The main types of tumor in 70 patients with invasive yeast infections were pancreatic carcinoma(12 cases),stomach carcinoma(11 cases)and hepatocellular carcinoma(7 cases).No relationship was found between tumor types and yeast infections with different species(P=0.804,P>0.05).There were 18 patients with tumor metastasis.Among them.9 patients had yeast bloodstream infections and 6 with yeast abdominal infections.Yeast bloodstream infections were more likely to be found in patients with tumor metastasis(χ~2=5.357,P<0.05).Seventy isolates of yeasts were all sensitive to triazoles.The MIC values of amphotericin B aginst 6 isolates of Candida and 2 Laurent cryptococcal were 2μg/ml.Conclusion Patients with tumor metastasis should be paid more attention for yeast invasive infections.and triazoles kept activity against yeasts tested.

Objective To evaluate the control effects on coal-burning-borne endemic fluorosis in Shangli County and Yuanzhou District of Jiangxi Province in 2014.Methods Self-evaluation at the county level was carried out in all diseased villages in Shangli County and Yuanzhou District,including 145 villages of Shangli County and 32 villages of Yuanzhou District.Review at the provincial level was based on the results of selfevaluation of the county,3 towns were chosen in each county (district),3 villages were chosen in each town,a total of 18 villages were re-checked in Shangli County and Yuanzhou District,and 10 households were chosen to recheck in each village.According to the project requirements for endemic disease control,in all diseased villages of Shangli County and Yuanzhou District,self-evaluation results of the county was used to evaluate the status of the improved and the used stoves,and dental fluorosis of children aged 8-12.Review at the provincial level was carried,and the results were evaluated based on the Standards for Control Criteria for Endemic Fluorosis Areas (GB 17017-2010) and Elimination of Coal-burning-borne Endemic Fluorosis.Results Self-evaluation of the county:110 443 households in Shangli County and 20 280 households in Yuanzhou District were investigated,the qualified rates of improved stoves were 92.45％ (102 109/110 443) and 98.28％ (19 932/20 280),respectively;the correct-usage rates of qualified stoves were 93.46％ (95 427/102 109) and 98.47％ (19 627/19 932),respectively.Dental fluorosis of 28 772 children were examined,the number of dental fluorosis was 5 730,the prevalence rate of dental fluorosis was 19.92％ (5 730/28 772);Shangli County and Yuanzhou District were up to the standard of fluorosis control,the rates of control were 95.17％ (138/145) and 100.00％ (32/32),respectively.Review at the provincial level:180 households were selected,the qualified numbers of improved stoves and the correct-usage of qualified stoves were 179 and 177,respectively,the qualified rate of improved stoves was 99.44％ (179/180) and the correct-usage rates of qualified stoves was 98.88％ (177/179).Dental fluorosis of 805 children were examined,the number of dental fluorosis was 139,the prevalence rate of dental fluorosis was 17.27％ (139/805).The consistency rate of county-level and provincial-level was 88.89％ (16/18).Conclusion The control efficiency for coal-burningborne fluorides in Shangli County and Yuanzhou District of Jiangxi Province is obvious,the prevalence is under control.

An optimized two-dimensional polyacrylamide gel electrophoresis (2-DE) system for analyzing plant proteins was developed by evaluating different reagents and concentrations used in the sample extraction solutions and lysis buffers. Two main sample preparation methods, referred to as trichloroacetic acid (TCA)-acetone method and phenol extraction-ammonium acetate/methanol (phenol-NH4Ac/methanol) precipitation method, were compared. Four ecotypes of reed plants (Phragmites communis Trin.) from the desert region of north-western China were used as experimental materials: (1) swamp reed (SR) which grows in water about 1 m deep; (2) dune reed (DR) which grows on 5～10 m high sand dunes; (3) heavy salt meadow reed (HSMR) which grows on low-lying salt flats; and (4) light salt meadow reed (LSMR) which grows in the transition area between DR and HSMR growing areas. The optimized phenol-NH4Ac/methanol precipitation method consisted of extracting leaf proteins of different ecotypes of reed with water-saturated phenol and then precipitating with a 5-fold volume of 0.1 mol/L NH4Ac in methanol, followed by dissolving in the lysis buffer. The optimized protein lysis buffer consisted of 7 mol/L urea, 2 mol/L thiourea, 4% CHAPS, 2% Ampholine(pH 3.5～10∶pH 5～8 = 1∶4) and 65 mmol/L DTT. The prepared protein sample (80 ?g) was then separated by 2-DE gel and detected by silver staining method. This improved 2-DE system resulted in a 2-D protein profile of higher resolution and higher protein yields as analyzed by PDQuest software. Good results were also obtained when this 2-DE system was used in 2-D analysis of proteins from other plant materials, such as rice leaves, indicating that it is a suitable 2-DE system for analyzing leaf proteins of different plant species.

90%),but they had low susceptibility rate to itraconazole(70.62%-50.45%).CONCLUSIONS The main species of fungi isolated from clinic is Can albicans which is susceptible to antifungal agents.It is important to give active treatment and choose antifungal agents reasonably for improving the prognosis and reduce drug-resistant strains.

Objective To compare several methods for the rapid detection of methicillin resistant StaphylOCOCCUS aureus(MRSA).Methods Forty-four Staphylococcus aureus isolates from Tianjin Medical University Cancer Institute and Hospital(TMUCIH)were detected by Oxacillin Kirby-Bauer disk diffusion method,Cefoxitin Kirby-Bauer disk diffusion method,Oxacillin micro-broth dilution method,E tests and PBP2a Latex agglutinaltion assay.These above results were compared with PCR analysis.Results PCR analysis showed that 36 MRSA strains containing mecA was identified.Thirty-two MRSA strains were detected by Oxacillin Kirby-Bauer disk diffusion method,Cefoxitin Kirby-Bauer disk diffusion method and Oxacillin micro-broth dilution method.Compared with PCR analysis,the sensitivity and specificity is 88.9%and 100%respectively.Thirty-three MRSA strains were identified by E test,with the sensitivity of 88.9%and specificity of 87.5%.Twenty-nine MRSA strains were identified by PBP2a latex agglutination assay with the sensitivity of 80.5%and specificity of 100%.Conclusions The turnaround time of PBP2a Latex agglutination assay could be reduced 24 h compared with other methods for detection of MRSA.This rapid,convenient and specific method could be applied in clinical laboratories for MRSA detection.

Objective To investigate the association of impaired glucose regulation and adiponectin (APN)with the clinical severity of coronary lesions.Methods A total of 210 cases of suspected coronary heart disease were examined by coronary artery angiography.The patients were differentiated as 4 groups:42 patients with normal glucose tolerance (NGT),36 patients with impaired fasting glucose(IFG),92 patients with impaired glucose tolerance (IGT;including 44 cases with postpraudial 2h plasma glucose(2 hPG) ＜ 10 mmol/L as IGT1 subgroup and 48 cases with 2h PG ≥ 10 mmol/L as IGT2 subgroup),and 40 patients with combination of IFG and IGT.Accordingly,body mass index (BMI),blood pressure,blood lipid,insulin,APN and CRP were measured to evaluate by Gensini score.Results The incidence of coronary heart disease and Gensini scores in IGT and IGT+IFG groups were significantly higher than those in either IFG or NGT subset(P＜0.05).APN in both IGT and IGT+IFG subsets was significantly lower than that in IFG or NGT subsets(P＜0.05),CRP values were significantly raised in both IGT and IGT+IFG subgroups compared with the other 2 subgroups(P＜0.05).Statistical difference in Gensini scores and APN was found between the 2 IGT subgroups (P＜0.05).Gensini scores were negatively correlated with APN level.Multivariate regression analysis showed that both APN and HOMA-IR values were independently correlated with the Gensini scores.Conclusion The lowered APN may serve as a more sensitive factor in predicting the coronary lesions in patients with IGR,especially in IGT cases.It woula be beneficial to cardiovascular complication by controlling the postprandial blood glucose level below 10 mmol/L.

Objective To report a Chinese boy suffering from nephrotic syndrome associated with Schimke immuno-osseous dysplasia (SIOD). Methods The clnical data and pathological changes of renal biopsy were analyzed and associated literatures were reviewed. The clinicopathological features and diagnosis of SIOD were discussed. Results The first symptom of the patient was recurrent infections. Growth retardation, spondyloepiphyseal dysplasia accompanied by nephrotic syndrome and defective cellular immunity were seen as clinical features in this patient. Renal pathology showed focal segmental glomerulosclerosis. Conclusion Combining the clinical manifestation with renal pathology, the case is diagnosed as Schimke immuno-osseous dysplasia.

Objective To investigate antibiotic resistance and staphylococcal cassette chromosome mec(SCCmec)genotype of Staphylococcus aureus isolated from patients with nosocomial infections.Methods Antibiotic susceptibility pattern anti the gene mecA were examined in 89 Staphylococcus aureus isolates from patients with nosocomial infection in Tianjin Medical University Cancer Institute and Hospital.The SCCmec genotype in the mecA-positive strains were fuaher investigated with multiplex PCR.Results In 89 Staphylococcus aureus strains,21(23.6%)were phenotypic methicillin-resistant Staphylococcus aureus (MRSA)and 39(43.8%)were rnecA positive,the difierence was of statistical significance(χ2=8.146,P=0.004).MRSA strains showed multiple-antibiotic resistance but sensitive to vancomycin.Tvpe Ⅲ SCCmec carriers were the major epidemiological strains(26/39,66.7%).ConclusionMRSA strains isolated from Tianjin Medieal University Cancer Institute and Hospital are characterized by multi-drug resistance,and type Ⅲ is the dominant SCCmec earried by the MRSA strains.

Objective The research about the effect of different chemotherapeutic drugs on CD19?CAR?T cells with CCK8 test to provide the theoretical support about the development of chemotherapy for clinical support. Methods Extract T cells from a normal adult peripheral blood and synthesize CD19?CAR?T cell. CD19?CAR?T cells were treated with different doses of chemotherapeutic drugs for 24,48,72 h and(or)96 h,and inhibition rate was calculated. Results First,we observed that the inhibition rates of fludarabine and Mafosfamide for CD19?CAR?T cells were increasing with the time and concentration (P < 0.05). Secondly , Bus ulfan had no effect in CD19?CAR?T cells in vitro(P > 0.05). Finally ,Cyclophosphamide had no effect in CD19?CAR?T cells in vitro (P > 0.05). Conclusion Mafosfamide and Fludarabine can inhibit the CD19?CAR?T cells. Cyclophosphamide have no activity in vitro.