Objective To investigate the expression and significance of endothelin-1 ET-1 in urothelial transitional cell carcinoma. Methods Immunohistochemistry was applied to detect ET-1 expression in 30 cases of urothelial transitional cell carcinoma and 20 cases of normal urothelial transitional cell samples, the results were analyzed in combination with clinical data. Results ET-1 expression in urothelial transitional cell carcinoma was 40.5%, which was significantly higher than that in normal urothelial transitional cell samples(P ＜0.05). ET-1 expression was correlated with grade and stage of urothelial transitional cell carcinoma (P ＜0.05). Conclusion The over expression of ET-1 in urothelial transitional cell carcinoma tissues correlates closely with cancer genesis progression and invasiveness, suggesting that ET-1 might be a biomarker and as a therapeutic target in human urothelial transitional cell carcinoma.

Objective:To study the short-term effect of early application of intestinal microecological therapy after gastric cancer surgery.Methods:A retrospective analysis was made on 96 patients with early and middle stage gastric cancer who underwent surgical treatment in the department of gastrointestinal surgery of Shulan (Hangzhou) Hospital from June 1, 2020 to May 31, 2021. Among them, 48 patients in the observation group were given enteral nutrition support treatment in the early stage after operation and intestinal microecological preparation, while the control group of 48 patients in the early postoperative were given enteral nutrition support. The serum total protein, albumin, prealbumin and other nutrition related indexes and immune related indexes such as CD4 + , CD8 + , CD4 + /CD8 + were detected before and 7 days after operation. The clinical indexes such as the time of first anal exhaust and the incidence of infectious complications were recorded. Results:There was no significant difference between the two groups in preoperative serum total protein, albumin, prealbumin and immune related indexes such as CD4 + , CD8 + , CD4 + /CD8 + ( P>0.05). 7 days after operation, the above indexes in the observation group were significantly higher than those in the control group (all P<0.05). The anal exhaust recovery time of observation group was faster ( P<0.05); There was no significant difference in the incidence of postoperative infectious complications between the two groups ( P>0.05). Conclusions:Early application of intestinal microecological agents in patients with gastric cancer after operation can significantly improve the nutritional status and immune function, promote the recovery of intestinal function, and will not increase the incidence of complications.

Objective? To analyze the application value of language communication and psychological support in urodynamic examination of patients with benign prostatic hyperplasia (BPH). Methods? From July 2016 to October 2018, a total of 106 BPH patients who underwent urodynamic examination in the Department of Urology at the First Affiliated Hospital of Kangda College of Nanjing Medical University were enrolled as subjects. They were divided into the observation groups and the control group by random number table method, with 53 cases in each group. The control group was given routine nursing. The observation group was given language communication and psychological support intervention besides routine nursing. The incidence of abnormal abdominal pressure changes, unnatural detrusor contraction, and unnatural external urethral sphincter contraction, examination time, and examination success rate were compared between the two groups. At 20 min before the start of examination (T1), 10 min after the start of examination (T2), and 10 min after end of the examination (T3), changes in hemodynamics indexes [systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR)]were also analyzed. The pain grading and incidence of complication during examination were compared too. Results? The incidence of abnormal abdominal pressure changes, unnatural detrusor contraction, and unnatural external urethral sphincter contraction in the observation group was lower than that in the control group (P＜0.05). The examination time in the observation group was shorter than that in the control group, while the examination success rate was higher than that in the control group (P＜0.05). At T2, SBP, DBP and HR in the observation group were lower than those in the control group. At T3, SBP and DBP in observation group were lower than those in control group (P＜0.05). The pain grade of the observation group was lower than that of the control group (P＜0.05). The incidence of bladder irritation, fever, hematuria and dysuria in the observation group was lower than that in the control group (P＜0.05). Conclusions? The application of language communication and psychological support in urodynamic examination for BPH patients can effectively improve examination efficiency, reduce hemodynamic fluctuations and pain, prevent or reduce complications risks so it is worth promoting in clinical practices.