Objective To observe the efficacy of montmorillonite combined with oxygen in the treatment of neonatal diaper dermatitis, and to provide the basis for clinical treatment. Methods Collected 100 cases of neonatal diaper dermatitis from January 2014 to January 2017 in our hospital. The patients were divided into observation group and control group according to the order of admission. After treatment, the observation group was given montmorillonite combined with oxygen therapy, the control group was given only oxygen treatment, compared the treatment effect of the two groups of patients. Results After treatment, the total effective rate of the observation group was 100％, the total effective rate of control group was 80％, there was significant difference between the two groups in the total effective rate of clinical treatment (P<0.05).The cure time was (116.20 ± 25.79) h in the observation group and (145.72 ± 24.88) h in the control group. The difference of the cure time between the two groups was statistically significant (P<0.05). In the observation group, the erythema was found in 42 children after treatment, and the area of erosion was obviously reduced before treatment, and the surface of the lesion was dry. After treatment for 5 days, 36 cases of mild to moderate diaper dermatitis were cured. After 7 days of treatment, children are cured. In the control group, 19 cases of mild diaper dermatitis were treated with erythema dermatitis . After treatment for 5 days, the area of hips in the 22 cases was smaller than that before treatment, and the lesion was dry. After 7 days, There was no significant improvement in erosion and or exudation. Conclusion Montmorillonite powder combined with oxygen treatment of neonatal diaper dermatitis is a significant effect, is the ideal treatment for neonatal diaper dermatitis.

Objective To explore different methods for establishing animal models of Ureaplasma urealyticum ( UU) infection and to select the best method of establishment of this disease .Methods SPF female BALB/c mice and Wistar rats were multiple-infected with small dose of UU liquid or single-infected with high-dose UU liquid, pretreated with estradiol benzoate, to establish models of Ureaplasma urealyticum infection.Cervical secretion was taken for UU culture at 14, 28, 42 and 56 days after the first time UU treatment , and the reproductive organs were taken for histopathological examination.Results Gross examination of the UU-infected rats showed generally less serious lesions than the mice .The low-dose estrogen group was worst , showing hyperemia and edema , cervical hypertrophy , hydrosalpinx , enlargement , and more rigid with poor elasticity .Significant differences were observed in the vaginal UU colonization rates between the low -dose estrogen and low-dose non-estrogen groups , high-dose estrogen and non-estrogen groups , low-dose estrogen and high-dose estrogen groups, low-dose estrogen and non-estrogen groups, in both the mice and rats (P<0.05 for all).The same dose estrogen treatment caused a higher UU colonization rate in the mice than rats ( P<0.05 ) .Histological examination showed that the low-dose estrogen treated rats had no obvious pathological changes , except loose tissue edema in some rats , but apparent pathological changes were observed in the low-dose estrogen treated mice .Conclusions The vulvar lesions in the mice are worse than in the rats .Estrogen pre-treatment can increase the Ureaplasma urealyticum colonization rate in the animals.The UU colonization rate in the vagina of mice is higher than in the rats , and multiple low-dose UU treatment produces a higher UU colonization rate than a single high-dose UU treatment.Therefore, BALB/c mice are more susceptible to Ureaplasma urealyticum infection, with more serious pathological changes , and are more suitable for experimental studies of related diseases .

Aim To study the anti-inflammatory effect of Yinhuang micro-enema compound (YHMEC) in vitro and its possible anti-inflammatory mechanisms. Methods Peritoneal macrophages (PM?) obtained from Wistar rats were randomly divided into four groups: control group, lipopolysaccharide (LPS)-induced group, YHMEC intervention group and dexamethasone intervention group. The morphological changes of cells were observed under convert microscope. Peritoneal macrophage viability was test with MTT. The levels of tumor necrosis factor?(TNF-?) and interleukin-6 (IL-6) were measured using ELISA. The translocation of nuclear factor-kappa B (NF-?B) p65 was detected with immunocytochemical method. Results The levels of TNF-? and IL-6 were increased significantly when PM? were induced by LPS and p65 were translocated from the cell cytoplasm into the nucleus. TNF-?,IL-6 secretions and translocation of NF-?B induced by LPS were inhibited by YHMEC. Conclusion The anti-inflammatory effects of YHMEC may act at least partly through inhititing the translocation of NF-?B and thus depress the expressions of TNF-? and IL-6.

BACKGROUND:Human and rat ovarian granulosa cels in dominant folicles have the phenomenon of expressing stem cel characteristics. OBJECTIVE:To investigate the expression of stem cel-related factors in rat ovarian granulosa cels. METHODS: After the paraffin sections of rat ovarian tissue, immunohistochemical method was used to detect CD34, CD133, ABCG2/Bcrp1, Pou5f1/Oct-4 expressions. Granulosa cels culturedin vitro were harvested by folicular puncture method, and then the immunohistochemical method was used to detect the expression of FSHR receptor in order to identify the purity of granule cels. In the cultured granulosa cels, CD44 and C-Kit expressions were detected immunohistochemicaly, RT-PCR was used to detect ABCG2/Bcrp1, Pou5f1/Oct-4, Nanog gene expressions in ovarian tissue and granulosa cels. RESULTS AND CONCLUSION:Immunohistochemistry detection on paraffin sections showed that a part of ovarian granulosa cels expressed CD34, CD133, ABCG2/Bcrp1 and Pou5f1/Oct-4, and the expression of Pou5f1/Oct-4 protein gradualy increased in the development of ovarian folicles, significantly enhanced during the luteal phase, and then disappeared after the formation of corpus albicans, displaying a periodic expression characteristics. FSHR receptor positive identification rate of primary cels harvested by the foliclar puncture method was more than 95%. Granulosa cels culturedin vitrowere mainly long spindle-shaped or diamond, and some cels presented with aggregation growth and expressed CD44 and C-Kit. RT-PCR test results showed that there were no Nanog in the ovarian tissue and cultured granulosa cels, low expression of Pou5f1/Oct-4 in the ovarian tissue, strong expression of ABCG2/Bcrp1 in the ovarian tissue, weak expression of Pou5f1/Oct-4 in the cultured granulosa cels, and strong expression of ABCG2/Bcrp1 in the cultured granulosa cels. These findings suggest that a part of granulosa cels in the rat ovarian have the characteristics of stem cels.