Objective To explore the mental-behavior characteristics in serious internet game addicts.Methods To investigate internet addiction questionnaire, EPQ, social support questionnaire, purpose in life test and sensation seeking scale in 30 serious internet game addicts ( age from 16 ～ 25 years) and 43 non-addicts.Results Internet addicts have obviously lower social support questionnaire score, subjective support score, purpose in life test score and sensation seeking TAS score than non-addicts( P<0.05 or P<0.01 ). Objective support score, the use score for social support and sensation seeking score can forecast the internet addiction extent of the internet addict group. Internet addicts have obviously lower L score of EPQ than non-addicts ( P<0.01 ). There is the negative correlation between N score of the internet addicts and sensation seeking score( r=-0. 452, P<0.05). Conclusions Serious internet game addicts have the metal characteristics of low social support, low purpose in life and low adventure; sensation seeking and social support scale have prognosticative function for internet addiction degree; serious internet game addicts of low N score have high sensation seeking characteristic.

Objective To analyze the clinical prognosis and its influencing factors in elderly patients with myelodysplastic syndrome.Methods A total of 36 elderly patients with myelodysplastic syndrome were included in this study,who were diagnosed and treated at our hospital from January 2013 to August 2016.The Kaplan-Meier method was used to analyzed the survival of these patients,and the effects of gender,age,WHO classification,white blood cell count,platelet count,hemoglobin and IPSS grouping on survival were analyzed.The independent risk factors associated with the clinical prognosis of patients were identified by using multivariate Cox regression analysis.Results Patients were followed up until March 31,2017 with a follow-up time ranging from 8 to 31 months.During the follow-up period,19(58.3％)deaths and 17(41.7％) survivals were reported.The average survival time was(19.17±1.47)months.Kaplan-Meier survival analysis showed that age,WHO classification,white blood cell count,platelet count and IPSS grouping were closely associated with survival in elderly patients with myelodysplastic syndrome.Multivariate Cox regression analysis showed that age and IPSS grouping were independent prognostic factors in elderly patients with myelodysplastic syndrome.Conclusions Age,WHO classification,white blood cell count,platelet count and IPSS grouping are closely related to the prognosis of myelodysplastic syndrome in elderly patients.

Purpose To investigate whether there is a difference in BRCA1 gene single nucleotide polymorphisms (SNPs) in Uighurs and Han Chinese sporadic breast cancer,and to analyze the relationship between SNPs locus and tumor susceptibility.Methods 100 cases of sporadic breast cancer (Uighur and Han 50 cases each) and 100 cases of mammary gland disease (Uighur and Han 50 cases each) were collected as the research object,the BRCA1 gene rs16941 and rs16942 were sequenced.Results The distribution of AA,AG and GG genotypes of rs16941 and rs16942 between Uygur and Han breast cancer groups were statistically significant (P =0.009,P =0.017).Compared with AA genotype of rs16941,AG genotype of rs16941 could reduce the risk of breast cancer in Uighurs (OR =0.964,95％ CI:0.260-3.583,P =0.009).Compared with AA genotype of rs16942,AG genotype of rs16942 could increase the risk of breast cancer in Uighurs (OR =1.017,95％ CI:0.293-3.916,P =0.017).Compared with AA genotype of rs16941,AG genotype could reduce the risk of breast cancer in Han nationality (OR=0.824,95％ CI:0.210-3.234,P =0.044).Conclusion The distribution of AA,AG and GG genotypes of rs16941 and rs16942 in Uygur and Han breast cancer groups are statistically significant,and SNPs is correlated with tumor susceptibility.

Objective To discuss the diagnostic value of soluble scavenger receptor(sCD163)for the patients of malignant tumor associated with fever in early infection .Methods Collect 102 cases of patients confirmed with malignant tumor in this hospi-tal since January to December 2014 ,all the patients were examined with blood culture .102 patients were divided into two groups ac-cording to the results of blood culture :infection group (60 cases) and uninfected group (42 cases) .ELISA ,electrochemical lumines-cence and immune transmission turbidimetric methods were used to detect the levels of sCD163 ,PCT and CRP in serum ,to compare the differences in the above indicators between two groups .The receiver-operating characteristic curve (ROC curve) was applied to evaluate the application value of sCD163 in diagnosing of malignant tumor associated with fever in early infection .Results The lev-el of sCD163 ,PCT and CRP had statistically difference in two groups(P< 0 .05) ;the critical values of sCD163 、PCT and CRP were 110 .80 ng/mL ,0 .45 ng/mL ,15 .60 mg/L respectively which can suggest the patients with malignant tumor complicated with fever in early infection .The area under the curve were 0 .894 ,0 .835 ,0 .743 respectively ,among that sCD163 area was the largest ;The corresponding sensitivity were 88 .9% ,77 .9% and 88 .0% ;specificity were 77 .0% ,74 .0% and 50 .0% respectively .Conclusion Compared with PCT ,CRP ,sCD163 has more value for diagnosing the patients of malignant tumor associated with fever in early in-fection .

Objective To explore the value of combined detection of bone marrow smear(BMS) and flow cytometry(FCM) to examine bone marrow involvement(BMI)of B cell non‐Hodgkin′s lymphoma(B‐NHL) .Methods Patients with B‐NHL were detec‐ted by BMS and FCM simultaneously .Smears were stained with Wright‐Giemsa′s staining to differentially count neoplastic cells and the processed sample with fluorescent staining for the FCM .Results 93 cases of patients with B‐NHL were detected .The detection rate of BMS and FCM were 11 .8% and 29 .0% .There was statistical difference of detection rate between the two method(P<0 .05) .In 11 cases patients of BMI with the method of BMS ,while using the method of FCM 10 cases were positive .Conclusion Both BMS and FCM are useful to diagnose BMI of B‐NHL ,each of them has individual characteristics .Combined detection of these two methods can increase the diagnostic of BMI .

Objective To develop a father involvement scale and test the reliability and validity.Methods The scale was developed based on previous studies and depth interviews with more than 20 fathers.Totally 1565 families from 3 provinces were recruited.Item analysis was conducted to identify valid items.Then the construct validity of the scale was tested by exploratory and confirmatory factor analysis (EFA & CFA).Finally the reliability and criterion validity of the scale was examined.Results The scale was consisted of 8 factors wihch were involvement attitude,activities and playing,accompany and taking care of children,attentiveness,discipline and school encouragement,economic support,protection,support mother.They explained 59.94％ of the total variance.Besides,the CFA results demonstrated a satisfactory construct validity of the scale (RMSEA =0.05,NFI =0.95,NNFI =0.96,CFI =0.97).The Cronbach(')s α coefficients of the scale was 0.897,and those of its 8 were 0.828,0.788,0.764,0.781,0.671,0.754,0.528 and 0.693 respectively.Conclusion The Father Involvement Scale with 8 factors has a good reliability and validity,demonstrating it a valid tool to measure father involvement in China.

Objective Queuing theory is the mathematical study of how waiting lines or queues are formed and dissipated o -ver time, which aims to work out the optimal design and optimal control of queuing systems based on the research of probability and regularity of various queuing systems .The aim of paper was to find out the maximum acceptable waiting time for outpatients in stomatol -ogy department and explore the rational allocation of dentists based on queuing theory model . Methods Questionnaires , worktime measurement and queuing theory model were applied to calculate indicators of queuing system in outpatient services of Stomatology De -partment , getting the maximum waiting time accpetable for patients and the reasonable number of dentists . Results The maximum acceptable waiting time for outpatients was 34.02 ±7.07 minutes, and it was reasonable to allocate 25 doctors in the morning and 16 doctors in the afternoon . Conclusion Applying queuing theory helps to optimize dentist number in outpatient services of Stomatology Department and provide scientific reference to improve medical efficiency .

Objective Corneal endothelial decompensation is caused by many corneal diseases. It often results in severe clinical complications. Endothelial keratoplasty (EK) is a new therapy for corneal endothelial decompensation. This study aimed to investigate a new approach to establishing corneal endothelial decompensation animal model with Descemetorhexis technique in order to better understand the tissue response to EK. Methods Thirty New Zealand white rabbits were randomly divided into three groups according to different surgical procedures; corneal endothelial cells (CEC), Descemet's membrane and corneal endothelial cells (DM + CEC) as well as Descemet' s stripping with endothelial keratoplasty(DSEK) group and 10 eyes for each. The right eyes of rabbits were as surgery eyes. Other 10 rabbits were as DSEK donors. Corneal transparency, anterior chamber response and graft location were examined once per day for two weeks under the slit lamp. Comeal thickness was measured by ultrasound biomicroscope. Corneal endothelial cells were analyzed using vital staining with alizarin red and trypan blue in 2, 4 and 8 weeks after operation. Results The cornea in DM + CEC group remained opaque throughout the observation period. In CEC and DSEK group, corneal clarity was gradually restored and corneal thickness was significantly less than that in the DM + CEC group during the postoperative 8 weeks. There were significant differences in corneal thickness between the DM + CEC group and CEC group or DSEK group during the postoperative 8 weeks (P ＜0. 05). The vital staining showed that most Descemetorhexis area was not covered by endothelial cells even 2 months after surgery. Conclusion A new corneal endothelial decompensation model is successfully established for the study of corneal endothelial keratoplasty, which is helpful for understanding the wound-healing of rabbit corneal endothelium after Descemel' s membrane damage.

Objective To evaluate the clinical efficacy of total hip arthroplasty with subtrochanteric femoral shortening osteotomy for Crowe Ⅳ developmental dysplasia of the hip.Methods From September 2003 to September 2012,21 patients (24 hips) underwent total hip arthroplasty with subtrochanteric femoral shortening osteotomy for Crowe Ⅳ developmental dysplasia of the hip in our hospital.There were 3 males and 18 females,aged from 28 to 71 years (average,54±10 years).The ceramicpolyethylene articulation was used in 17 patients (20 hips),and metal-polyethylene articulation in 4 patients (4 hips).The osteotomy site was treated with autologous bone graft in 18 patients (21 hips) and allogeneic bone graft in 3 patients (3 hips).The Harris hip score was used to assess the clinical results.Results A total of 18 patients were followed up for 0.5 to 9 years (average,3.5 years).The Harris hip score was improved from preoperative 47.9±9.1 to 88.4±3.5 at 6 months postoperatively.For most patients,hip pain relieved significantly; range of motion of the hip was improved,and the gait returned to normal.Sciatic nerve palsy occurred in 1 patient.There was no wound infection.X-rays 6 months after operation showed that the position of prostheses was satisfactory,without loosening of prostheses and bone block resorption.Conclusion Total hip arthroplasty with subtrochanteric femoral shortening osteotomy can achieve good clinical effect in Crowe Ⅳ developmental dysplasia of the hip.Moreover,it can improve leg length discrepancy and decrease the risk of sciatic nerve injury.

Objective To observe vasculogenic mimicry formation difference in human hepatocellular carcinoma cell lines MHCC97-H/L with different metastatic potentials under three-dimensional culture condition,and to study extracellular matrix and adhesion molecules-related mechanism of vasculogenic mimicry.Methods Three-dimensional cell culture system of MHCC97-H/L was established to observe tubelike structures by inverted microscope.Human umbilical vein endothelial cell(HUVEC),human hepatocellular carcinoma cell line Hep3B with non-metastatic potentials and normal human hepatic cell line HL-7702 were taken as control.Gene expression profiles of MHCC97-H and MHCC97-L were detected by Oligo extracellular matrix and adhesion molecules microarray analysis.Two differentially expressed genes were verified with semi-quantitative reverse transcriptionpolymerase chain reaction(RT-PCR)and Western blot.All data were analyzed using two sample t test.Results After a 24-hour three-dimensional culture,the length of tubelike structures was(474 ± 16)mm/cm2 in MHCC97-H,which were significantly longer than(320 ±41)mm/cm2 in MHCC97-L(t =6.119,P ＜0.05).Hep3B and HL-7702 could not form tubelike structures.Compared with MHCC97-L,the expression of 7 genes were up-regulated and the expression of 3 genes were down-regulated among a total of 113 angiogenesis genes in MHCC97-H.Two of the genes with differential expressions including tenascin C and extracellular matrix protein 1 were further validated by RT-PCR and Western blot.Conclusion MHCC97-H has stronger ability to form vasculogenic mimicry than MHCC97-L,and this perhaps correlates with the differential expression of certain extracellular matrix and adhesion molecules-related genes in MHCC97-H.