Objective To investigate the factors of lifestyle associated with the mild cognitive impairment (MCI). Methods A 1∶ 2 matched case-control study investigated 120 patients of MCI and 240 healthy controls with lifestyle factors associated with MCI. Results Multiple factors analysis confirmed that, participate in social activities (OR=0.562, 95%CI: 0.359~0.723), playing chess or joke (OR=0.603,95%CI: 0.323~0.884), travelling (OR=0.683, 95%CI: 0.467~0.912), continue to work after retirement (OR=0.531, 95%CI: 0. .326~0.724),reading (OR=0.272, 95%CI: 0.174~0.428), using computer (OR=0.536, 95%CI: 0.427~0.869), regular physical exercise (OR=0.437, 95% CI: 0.316~0.732), friendship (OR=0.583, 95%CI: 0.385~0.765), meeting children (OR=0.464, 95%CI: 0.316~0.745), intaking unsaturated fatty acids (OR=0.524, 95%CI: 0.368~0.787), often eating fish and seafood (OR=0.224, 95%CI: 0.171~0.383), daily consumption of fruits and vegetables (OR=0.485, 95%CI: 0.316~0.665) were protective factors for MCI. Conclusion A positive, leisure lifestyle, health reasonable diet and avoiding risk factors could effectively reduce the risk of MCI.