OBJECTIVE:To study the preparation of the ligustrazine hydrochloride liposome and evaluate its quality.METHODS:The liposome was prepared by various methods on a trial basis.The entrapped efficiency(EE)of the liposome derived from passive loading and active loading was compared.RESULTS:The ammonim sulfate gradient technology had the highest EE of 48.63%and its mean size was 6.5?m and the pH was 5.93.It was stable in refrigeratory(4℃～10℃)storage for 30 days.CONCLUSION:The ammonim sulfate gradient technology of preparation of the ligustrazine hydrochloride liposome is feasible.

Objective To explore the role of glutamate decarboxylase (GAD) in the pathogenesis of human gastric cancer. Methods Thirty patients with primary gastric cancers who were diagnosed and treated at our hospital were enrolled into the current study. Immunohistochemistry was carried out to check the ex-pression of GAD protein. Reverse transcription polymerase chain reaction ( RT-PCR) was performed to ex-amine the expression of GADmRNA. Then investigated the relationships among the sites of gastric cancer, depth of infiltration, and degree of differentiation, staging and lymphatic metastasis. Results Immunohisto-chemistry analysis showed that GAD expression decreased in gastric cancer tissues compared with that of ad-jacent normal tissue(P