1.Advancement of Quorum Sensing in Rhizobia
Jun GU ; Wen-Feng CHEN ; Qiang CHEN ; Wen-Xin CHEN ;
Microbiology 1992;0(06):-
Quorum sensing is defined as the cell density-dependent regulation of gene expression, and the involved system is the quorum sensing system, in which N-acyl homoserine lactone is known as the signal molecules of most gram-negative organisms. It can regulate diverse physiological functions. This paper reviewed the quorum sensing systems and the recent advances which play a major role in the formation of the symbiosis between the rhizobia and their host plants.
3.COMPARATIVE ANALYSIS OF NUMERICAL TAXONOMY AND 16S rDNA PCR-RFLP OF FAST-GROWING RHIZOBIA ISOLATED FROM WESTERN CHINA
Feng-Ling KAN ; Wen-Xin CHEN ;
Microbiology 1992;0(03):-
strains of fast-growing rhizobia isolated from Chinas western (mainly from Qinghai province),and 4 representative strains were compared by performing numerical taxonomy. 132 phenotypic characteristics were analyzed. The results of numerical taxonomy constructed a dendrogram. It shows that all the strains examined clustered into five phena at a similarity level of 79%. 16S rDNA PCR-RFLP were applied to 57 rhizobial strains (among the 61 strains) and 10 reference strains. The 16S rDNA-RFLP analysis described 20 different genotypic characteristics and form one dendrogram. For some large groups, the results of 16S rDNA-RFLP were agreement with that of numerical analysis of phenotypic characteristics.
4.Baculoviruses as Vectors in Mammalian Cells
Chang-yong, LIANG ; Xin-wen, CHEN
Virologica Sinica 2007;22(2):148-157
The Baculoviridae are a large family of enveloped DNA viruses exclusively pathogenic to arthropods. Baculoviruses have been extensively used in insect cell-based recombinant protein expression system and as biological pesticides. They have been deomostrated to be safe to mammals, birds and fish. Recently, baculoviruses has been shown to transduce different mammalian cells in spite of the fact that they cannot replicate in mammalian cells (11, 73, 76). This has resulted in the development of baculoviruses as mammalian expression systems and even as vestors for gene therapy.
6.National Comprehensive Cancer Network, Americal Society of Clinical Oncology and Oncology Nursing Society in the guide nursing enlightenment to our country related content
Xin PENG ; Qu WEN ; Xiaomeng LI ; Fan LIANG ; Wei CHEN
Chinese Journal of Practical Nursing 2015;31(14):1076-1079
We aimed to make comprehensive cancer care guidelines of China according to American guidelines of National Comprehensive Cancer Network,Ametican Society of Clinical Oncology and Oncology Nursing Society,and to guide clinical nurses to study and draw lessons from foreign latest guidelines,and combined with the actual situation in our country.The results of study and application were advantageous to the nursing profession,the security and interests of the patients.We should develop comprehensive cancer care guidelines of our country,eventually make nursing practice guide system of China constantly improve and perfect.
7.Clinical application of RapIDYeast Plus system in identifying 150 clinically common yeasts
Hong XU ; Hai WEN ; Jianghan CHEN ; Xin HUANG
Academic Journal of Second Military Medical University 1982;0(02):-
Objective:To study the role of RapIDYeast Plus (RYP) system in identifying common clinical yeasts.Methods: The target strains were cultured and passaged twice on Sabouraud dextrose agar,and were fed to RYP system after 24-48 h incubation at 30℃.Results: One hundred and thirty-nine of the 150 target strains were identified to the level of species correctly,and 8 undetermined strains were confirmed by additional tests.It was found that 3 strains had been incorrectly identified by RYP system.The accuracy of RYP system was calculated as 92% without additional tests and 98% with additional tests.Conclusion: RYP system is suitable for routine tests in clinical microbiological laboratory;it can accurately identify more than 40 kinds of yeasts and yeast-like bacteria in clinical practice.