Objective To investigate the influence of chiropractic therapy on the growth and intellectual development of 6 months infants.Methods 160 cases of 1-month-old full-term normal infants were divided into the chiropractic therapy group and the control group,each group contained 80 samples.Chiropractic therapy was given to the chiropractic therapy group daily.The development of weight,length,head circumference were observed,and the intelligent development for two groups were evaluated at the age of 6 months.Results The weight,body length in the chiropractic therapy group at the sixth month were higher than those in the control group.The intellectual development in the chiropractic therapy group was also higher than that in the control group.Conclusions Chiropractic therapy plays a significant role in promoting the physical and mental development of babies of 6 months,which is worth being promoted.

Objective To discuss the discovery of the neurbehavioral examination to premature infants in early abnormal neurobehavior and evaluate the influence of the hospital and family intervention pattern on the growth and development of the premature infants,so as to provide reference to the abnormal test of the premature infants and discuss the feasibility of developing neurobehavioral examination widely.Methods 152 cases of the intervention group were given neurobehavioral measurement after correction age,when the score ＜37 points,intervention and treatment were suggested in regular hospital (including exercise therapy and physical therapy,electrical nerve stmulation with treatment instrument,etc.).When the score ≥ 37points,personalized training plans were provided at home,which was adjusted in the follow-up.138 cases of the control group were given development assessment and neurobehavioral examination 3 months after correcting of age.Results The abnormal detection rate of the nervous system in the control group was higher than that of the intervention group,the scores of development in the control group were lower than that of the intervention group.Conclusions NBNA check is simple,safe and strong,it can reduce the occurrence of abnormal nervous system,and improve the level of intellectual and motor development.

OBJECTIVETo observe the effect of Wenyang Huoxue Lishui Recipe (WHLR) containing serum on the expression of cathepsin L (CatL) in puromycin aminonucleoside-induced injury of mouse glomerular podocytes.

METHODSMouse podocyte cells (MPCs) in vitro cultured were divided into the normal control group, the model group, the dexamethasone (DEX) group, 10% WHLR containing serum group, 20% WHLR containing serum group, the vehicle serum control group. MPCs in the normal control group were cultured at 37 degrees C culture solution for 24 h. 45 mg/L puromycin was acted on MPCs in the model group for 24 h. On the basis of puromycin intervention, 1 limol/L DEX was co-incubated in MPCs of the DEX group for 24 h; 10% or 20% WHLR containing serum was co-incubated in MPCs of the 10% WHLR containing serum group and 20% WHLR containing serum group for 24 h. The vehicle serum control group was also set up by incubating with WHLR containing serum alone for 24 h. The expression of CatL and its substrate Synaptopodin in podocytes were detected by cell immunofluorescence staining. FITC-conjugated phalloidin was used to stain F-actin. A cortical F-actin score index (CFS index) was designed to quantify the degree of cytoskeletal reorganization in cultured podocytes.

RESULTSCompared with the normal control group, the expression of synaptopodin significantly decreased and the expression of CatL significantly-increased in the model group. F-actin arranged in disorder, gradually forming pericellular F-actin ring. CFS index was obviously elevated (P < 0.01). Compared with the model group, the epression of synaptopodin increased, the expression of CatL decreased, and CFS index also decreased in the DEX group, 10% WHLR containing serum group, and 20% WHLR containing serum group (P < 0.05, P < 0.01). Compared with the DEX group, the expression of synaptopodin decreased in 10% WHLR containing serum group, CFS index also decreased in 20% WHLR containing serum group (P < 0.05).

CONCLUSIONSWHLR could up-regulate the expression of synaptopodin, down-regulate the expression of CatL, and alleviate cytoskeletal reorganization of F-actin. It was helpful to stabilize the cytoskeleton of F-actin and improve the merging of podocytes.

Actins ; metabolism ; Animals ; Cathepsin L ; metabolism ; Cells, Cultured ; Down-Regulation ; Drugs, Chinese Herbal ; pharmacology ; Kidney Glomerulus ; cytology ; Mice ; Microfilament Proteins ; metabolism ; Podocytes ; drug effects ; pathology ; Puromycin Aminonucleoside ; adverse effects ; Up-Regulation

Congenital lower urinary tract obstruction in the fetus and is rare in neonates,but it is identified in-creasingly in the era of prenatal sonography and resulted in substantial perinatal mortality with lifelong morbidity. Poste-rior urethral valves,urethral atresia,and prune - belly syndrome are the frequently reported causes of lower urinary tract obstruction. Despite the completion of a randomized trial,the benefit of prenatal intervention with vesicoamniotic shunt placement remains unclear. The management of these disease in fetus and newborns requires appropriate urinary tract decompression,subspecially support,and definitive diagnosis with endoscopy and radiography. Now,the recent progress of diagnosis and treatment of congenital lower urinary tract obstruction were reviewed.

Large number of TCM ancient books, the literature, has a long history and complicated contents, including the principles, methods, prescriptions, medicines and other various aspects. How to obtain and make use of knowledge in ancient books, we must establish a set of relatively precise knowledge and semantic relation networks. Knowledge yun performing reasonable rebuilding of TCM literature, containing much TCM knowledge and information, will be an important way for knowledge findings.

Objective To investigate the effects of 0.9 g/dl NaCl diluting instrument method to solve the ethylenediamine tet-raacetic acid dipotassium (EDTA)anticoagulant dependency pseudo reduce platelet syndrome (PTCP)feasibility,provides solutions to clinical laboratory PTCP more effective method.Methods From August to October of 2014 in their laboratory for PTCP cases in all 3 cases,2 ml venous blood in EDTA and citron acid sodium anticoagulation in-line blending,in the im-mediate,10,30,40 and 60 min computer detection.Collected of peripheral blood in blood thinners,respectively,0.9 g/dl NaCl solution blending,in the immediate,10,30,40 and 60 min computer detection,and compared with the manual method of ammonium oxalate.Results EDTA,citron acid sodium,blood thinners and 0.9 g/dl NaCl diluting instrument immediately detected PTCP blood PLT result compared with ammonium oxalate method,there were no statistically significant difference (t=0.943~1.537,P >0.05),10 min~60 min anticoagulant blood PLT results significantly decreased,compared with am-monium oxalate method difference had statistical significance (t = 12.413 ~ 12.413,P <0.01 ~0.001).Citron acid sodium PLT began to decline after 30 min,compared with ammonium oxalate method,the difference was statistically significant (t=4.915~4.915,P <0.05~0.01).Blood dilution method in 30~40 min PLT test results began to decline,but not obvious, there was no statistically significant difference with the method of ammonium oxalate (t=1.315~1.715,P >0.05),40~60 min PLT test results appear significantly decreased,and the method of ammonium oxalate difference was statistically signifi-cant (t=3.175~3.175,P <0.05);Within 0~60 min 0.9 g/dl NaCl method to detect the PLT differences between the re-sults with the method of ammonium oxalate had no statistical significance (t=0.694~ 1.062,P >0.05).Conclusion ED-TA,citron acid sodium,blood thinners and 0.9 g/dl saline diluting instrument immediately detected PLT PTCP patients were consistent with ammonium oxalate method.Citron acid sodium within 30 minutes and blood dilution method in patientswith PTCP PLT detection could achieve ideal effect,but there were still a small amount of PLT gathered and led to a slight drop in PLT.0.9 g/dl saline diluting instrument method with ammonium oxalate within 0~60 minutes method to detect the PLT result had no difference.

Objective:To Integrated PACS and hospital information systems (HIS), and improve the efficiency of PACS, and improve the user experience. Methods:Base on deeply analysis of existing problems in system application, using process optimization, software optimization, and hardware upgrades manner optimize PACS. Results: PACS and HIS realized Integrated, improving the overall performance of the PACS. Enhance the user experience and expand the application scope of PACS. Conclusion: Deeply analysis of existing problems should be done before optimizing the system. Process optimization, software environment optimization should be considered as the most important and upgrading and replacement of the hardware as the secondary.

Ischemic cerebrovascular disease accounts for about 70％ of all types of stroke,and large vessel occlusive stroke is a major subtype of ischemic stroke.Currently,endovascular revascularization has been widely used in the treatment of large vessel occlusive stroke.The development of multi-modal neuroimaging has provided technical support to this.This article reviews the roles of neuroimaging in endovascular recanalization in patients with ischemic stroke.

Objective To investigate the morphology and bonding of enamel and dentin conditioned by 35% phosphoric acid and pulse Nd:YAG laser.Methods A total of 52 human premolars were selected,40 of which were divided into four groups randomly: group A [enamel treated with 35% phosphoric acid(Scotchbond,3M) for 30 s];group B [enamel treated with pulse Nd:YAG laser(Evlaser Friendly A4 Italy) for 20 s];group C(dentin treated with 35% phosphoric acid for 15 s);group D(enamel treated with pulse Nd:YAG laser for 15 s).The tensile bond strength(TBS) were determined.Except 4 out of 12 human premolars without any treatment,8 were divided into 4 groups and were treated with the method mentioned above.The surfaces of 12 human premolars were observed under SEM.Results The highest TBS was obtained in group A in comparison with the other 3 groups(P0.05).The smear layer on enamel and dentin could be removed after conditioned by both phosphoric acid and pulse Nd:YAG laser.The honeycomb structures were observed on all enamel but less regularly in lased group.Almost all the orifices of dentinal tubules were open in the acid group and sealed or half-sealed in lased group.Conclusion The pulse Nd:YAG laser did not contribute to bonding of enamel,but could improve the bonding of dentin and seal most of the orifices of dentinal tubules,which reduced the harmful stimulation to the dental pulp.