Objective To study the role of cyclcoxygenase-2 in the growth and development of the endome-trial lesions. Methods The expression of cycloogxygenase-2 (COX-2) were tested by immunohistocbemistry. Compar-ison of endometrial cancer COX-2 expression was made within each group at different stages,for different pathological classification. Another groups comparison of COX-2 expression was made. Results ( 1 ) COX-2 in endometrial cancer in the different expression of other differences and differences were significant ( P<0.05 ). Ⅲ mainly for the period between the stage and the other had a significant difference( P<0.05 ). (2) COX-2 in endometrial cancer,hyperplasia and normal expression of the differences between endometriosis, the difference was significant ( P<0.05 ). (3)COX-2 in endometrium cancer different pathology graduation difference:G1(high idiophase) < G2(idiophase) < G3(low id-iophase), but does not have the difference after the comparison in statistics ( P>0.05 ). (4) The expression of COX-2 in simple hyperplasia and complex hyperplasia was statistically significant different ( P<0.05 ). Conclusion That COX-2 in the development of endometrial lesions play an important role, it could be inferred of COX-2 inhibitors for endometrial cancer treatment or treatment of postoperative the timing of the loss surgery patients is feasible.

Objective To study the relationship between survival rate and pathogen,subtype,stage,delivered methods of 55 patients with severe hepatitis during late period of pregnancy.Methods Clinical data of 55 cases with severe hepatitis during late period of pregnancy was analyzed retrospectively.Results The incidence of hepatitis B was highest in patients with severe hepatitis during late period of pregnancy (84.5%).In subtypes,the incidence rates of chronic severe hepatitis,acute severe hepatitis and subacute severe hepatitis were 83.6%,5.5%,10.9% respectively(P

The traditional medical treasures of zhouyi and qianjin have much in common in the aspects of logic approach,recognition characteristics,values conception,theory construction,and category system,which demonstrate that the two doctrines are closely related both geographically and historically. Bearing the same cultural genes and value orientation,sharing similar constructions of logic approaches and recognition characteristics,the two doctrines contain a great deal of wisdom in recognition and practice.

Objective To investigate the infection type,clinical features and prognosis in the infections complication patients with severe hepatitis.Methods 72 severe hepatitis patients with infections complication were analyzed retrospectively.Results The infection occurrence rate was 62.5% in the 72 severe hepatitis patients.Mortality rate was 75.56% in the patients with infections complication,and it was significantly higher than those without infections complication(29.63%).The mortality rate was 72.5% in the patients with multiple organ infections.The different bacterium infection could lead to different mortality rate,K.peneumoniae(69.89%),E.coli(58.12%),Staphylococcus aureaus(55.56%),Staphylococcus epidermidis(59.90%).The mortality rate was higher in the patients with lower sera albumin and higher serum bilirubin level.Conclusions The patients with severe hepatitis have bad outcome with infections complication.The prognosis is correlated with the dysfunction severity of liver and pathogenic bacteria.

Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2(YFP-WT-TRAF2) or YFP fusion dominant-negative TRAF2(DN-TRAF2),or transfected with shRNA-TRAF2 and control shRNA plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by antibiotics G418.Activation of AP-1 pathway,including phosphorylation of ERK,JNK and P38,as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA.Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38,and further induced nuclear translocation of C-FOS.Moreover,both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38,and nuclear translocation of C-FOS.Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway,and plays an important role in AP-1 activation.

Ten compounds, including five steroidal saponins and five flavonol glycosides, were isolated from the whole plant of Solanum coagulans by means of column chromatographies over silica gel, ODS, Sephadex LH-20, and preparative HPLC. Based on analysis of MS and NMR spectroscopic data, their structures were established as anguiviosides XV (1), smilaxchinoside A (2), methylprotodioscin (3), protodioscin (4), solamargine (5), 3', 4', 5-trihydroxy-7-methoxy-6-C-β-D-glucopyranoside (6), brainoside B (7), camsibriside A (8), kampferol 3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (9), and quercetin-3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (10). All the compounds were first isolated from this plant. In the in vitro assays, compounds 4 and 5 showed cytotoxic activity against SMCC-7721 and NCI-H460.
Cell Line, Tumor ; Flavonols ; chemistry ; isolation & purification ; pharmacology ; Humans ; Saponins ; chemistry ; isolation & purification ; pharmacology ; Solanum ; chemistry

Adult ; Bronchoalveolar Lavage ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; therapy ; Pulmonary Disease, Chronic Obstructive ; etiology ; therapy ; Treatment Outcome

Cervical Intraepithelial Neoplasia ; diagnosis ; epidemiology ; prevention & control ; virology ; China ; epidemiology ; Female ; Humans ; Mass Screening ; Nucleic Acid Hybridization ; methods ; Papillomaviridae ; isolation & purification ; Papillomavirus Infections ; diagnosis ; prevention & control ; Papillomavirus Vaccines ; Uterine Cervical Neoplasms ; diagnosis ; epidemiology ; prevention & control ; virology ; Vaginal Smears

Objective To study the effects of body weight status in school-age children on later development of physcial growth , physcial growth ,and blood pressure .Methods Physical examination records of schoolchildren completed in 2005 and in 2009 in one district of Chongqing was used by information matching to identify the same person in two examinations .The body weight in former examination was grouped according to the national reference of weight by age ;the effects of this body weight level on the changes of weight ,height ,and blood pressure between two examinations were analyzed .Results There were 10 373 subjects aged from 6-14 years old were linked between two examinations .Results showed that in younger children underweight was associated with a higher increasments of weight and height .The increment of blood pressure was negatively associated with weight in younger children but this relationship disappeared or reversed in older children .The increment of blood pressure was also positively associated with the weight gain in all children ,no effects of height gain on blood pressure was founded .Conclusion Underweight usually has a catch up development of weight and hieght before puberty ,underweight in younger school age ,more weight gain and overweight in older school age mihgt be the risk factors of hypertension .

Objective To observe the imaging features of branching vascular network (BVN) in polypoidal choroidal vasculopathy (PCV).Methods Eighty PCV patients (90 eyes) were enrolled in this study.The patients included 58 males and 22 females.The age was ranged from 49 to 85 years,with a mean age of 61.4 years.All the patients were examined for fundus photography,fundus fluorescein angiography (FFA),indocyanine green angiography (ICGA) and optical coherence tomography (OCT).The fibrovascular retinal pigment epithelium detachment (PED) was defined as a well-demarcated subretinal heterogeneous plaque with increasing fluorescence on FFA.The late lichenoid hyperfluorescent plaque was defined as a well-demarcated lichenoid hyperfluorescent plaque on late phase ICGA.The double-layer sign on OCT was defined as a wide range of shallow PED from Bruch membrane.Results BVN were found on early ICGA in 76 eyes among the 90 eyes (84.4％).Among these 76 eyes,18 eyes (23.7％) demonstrated the subretinal reddish-orange branches corresponding to BVN.Fifty-six eyes (73.7％) demonstrated all or part of the BVN on early FFA.Three eyes (3.9％) demonstrated branching transmitted fluorescence corresponding to BVN throughout the FFA.Seventy-three eyes (96.1％) were manifested by occult choroidal vascularization on FFA,and 21 eyes (27.6％) of them were fibrovascular PED.Among the 76 eyes with BVN,all BVN appeared earlier than polypoidal lesions on ICGA.Polypoidal lesions located on the terminal of BVN in 62 eyes (81.6％).Sixty-nine eyes (90.8％) on ICGA demonstrated the late lichenoid hyperfluorescent plaque,whose area was equal to or greater than the area of BVN shown on early ICGA.Seventy-two eyes (94.7％) had the double layer sign.Among these 72 eyes,15 eyes (20.8％) had lumen like structure within the double-layer sign.Sixty-five eyes (90.3％) had punctate and linear hyperreflectance within the double-layer sign.Two eyes (2.8％) demonstrated a hyporeflective short segment and a gap of Bruch membrane on OCT corresponding to the origin of the BVN.Sixty-three eyes (87.5％) had an area of double-layer sign that matched the area of late lichenoid hyperfluorescent plaque on ICGA.Conclusions BVN in PCV can be noted as reddish-orange branches on fundus examination.Most of the BVN are shown as early branching transmitted fluorescence but collectively an occult choroidal vascularization on FFA,as lichenoid hyperfluorescent plaque on late ICGA,and as double-layer sign on OCT whose area matches late lichenoid hyperfluorescent plaque.