1.Analysis and Safety Evaluation of the Clinical Efficacy of Laparoscopic Appendectomy for Acute Appendicitis
Journal of Kunming Medical University 2016;37(8):119-122
Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P<0.05);there was no significant difference in lymph nodes (t=0.570,P>0.05);the amount of bleeding was less than that of the control group (t=20.748,P<0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P<0.05);eating time was faster than the control group (t=8.794,P<0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P<0.05);hospital stay was shorter than the control group (t=13.479,P<0.05);the complication rate was lower than that of the control group (t=13.883,P<0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P<0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P<0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P<0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.
2.Study on WAN Mi-zhai's health preserving thought
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(10):-
The WAN Mi-zhai's health preserving thought includes guayu,shendong,fashi and queji .The key of guayu is to control appetition to conserving essence.The aim of shendong is to keep the good psychology with peaceable mind.The view-point of fashi is to keep up with nature for harmonizing yin and yang.The denotation of queji is to prevent disease and regulate essence, qi and spirit.
3.Experimental Study on Dunhuang Nourishing Cream in Delaying Skin Aging
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(03):-
Objective To observe the influence of Dunhuang Nourishing Cream on the contents of SOD, GSH-PX and MDA in skin of aged rats. Methods Female SD rats of SPF level were divided into 6 groups:high, moderate and low dose groups of Dunhuang Nourishing Cream, model contrasting group, positive contrasting group and blank contrasting group. After removed the hair on the back, the drug groups were applied with Dunhuang Nourishing Cream, the model group was applied with the matrix of Dunhuang Nourishing Cream and the positive group was applied with vitamin E. After 30 days, the contents of SOD, GSH-PX and MDA in the skin were tested with the method of biochemistry analysis. Results Compared with the model group, the contents of SOD, GSH-PX and MDA in the high, moderate and low dose groups had significant difference (P
4.Progress in Studies on Interactive Toxicity of Organophosphorus Insecticides
Journal of Environment and Health 2007;0(07):-
The organophosphorus insecticides including a large group of insecticides were widely used in the world in the agriculture and household. Over 80% of the pesticides used in China are organophosphorus pesticides. Workers and the general population may be exposed to insecticides mixtures through water,air and the residue of food. After entering into human body,the insecticide mixtures can cause a combined toxicity. In this paper,the effects and toxicity mechanisms of combined exposures on health were expounded.
5.The Construction of the Humanity Quality Cultivation System for Medical Students
Xiaojin ZHUANG ; Lixian PAN ; Wen SU
Chinese Journal of Medical Education Research 2003;0(03):-
We regenerate and inaugurate the humanity quality cultivation modes of medical students.Based on the principles of scientificity,feasibility,openness,flexibility,multiplication and so on,we construct an effective training system for medical students to further improve their humanity quality.
6.Application of Modern Separation Technologies in Antibiotics Extraction
Microbiology 1992;0(05):-
The development of membrane separation technology in antibioti cs extraction was presented. The application of high performance capillary electr ophoresis, aqueous two-phase system and reverse micelles extraction were also b riefly introduced. Besides, the foreground of these modern separation technolog ies was discussed.
7.The dynamic observation of the levels of IL-2,IL-5 and IL-6 produced by Balb/c mice infected with Dengue Virus type Ⅱ clinic strain
Yu PAN ; Li ZUO ; Wen-Jie CHEN ;
Chinese Journal of Infectious Diseases 1997;0(04):-
Objective To observe the dynamic levels of IL-2,IL-5 and IL-6 produced by Balb/ c mice infected with DEN_2 clinical strains and to study their relation.Methods The Balb/c mouse in- feetion model was established by multiple-site subcutaneous injection with various doses of DEN_2 clini- cal strain.Mouse plasma samples collected from different experiment groups at various time after in fection were tested for IL-2,IL-5 and IL-6 levels with sandwich ELISA.Results After primary in- feeted with DEN_2 B strain,the levels of IL-2,IL-5 and IL-6 of all experimental groups were not sig- nificantly higher than the normal control group while the levels of experimental groups increased sig- nificantly after re-infection.The level of IL-2 reached to peak[average value of(101 522.44?10 465.375)pg/ml]at the 4th day after re-infection(the 20th day after the primary infection),and then the level gradually reduced.The levels of IL-5 in the Balb/c mice of the group 1 and 2 reached to peak at the 1st day after re infection(the 16th day after the primary infection),and there was signifi- cant difference between these two groups and the control group(P<0.05).The levels of IL-6 in all experimental groups reached to peak at the 1st and the 2nd day after re-infection.The peak value of the third group is the highest comparing with the normal control group(P<0.05).Conclusion Th2 response was predominant in the second infection phase.
8.Change of Cytochrome Oxidase Expression in Type Ⅱ Alveolar Epithelial Cell of Rats Exposed to Hyperoxia
rui, PAN ; li-wen, CHANG ; yan, CHEN
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To observe the expression of mitochondrial encoding cytochrome oxidase subunitⅠ(COXⅠ) and subunitⅡ(COXⅡ) in type Ⅱalveolar epithelial cell(AEC Ⅱ) of rats exposed to hyperoxia and explore the role of COXⅠand COXⅡ in hyperoxia-induced lung injury.Methods AECⅡ were gained by primary culture from 19-days fetal rats lung.After purified,AECⅡ were randomly assigned to hyperoxia group and air group.Hyperoxia group was flushed the flake with 950 mL/L O2 and 50 mL/L CO2 at 3 L/min for 10 min,then sealed.Both groups were in CO2 culture chamber(37 ℃,50 mL/L CO2).After 6,12 and 24 hours of exposure,AECⅡ were harvested and extracted for total RNA.COXⅠand COXⅡ mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).The results were analyzed by SPSS 12.0 softwore.Results Compared with air group,COXⅠmRNA expression in hyperoxia group increased significantly at 6 hours(t=3.832 P=0.019) and 12 hours(t=10.431 P=0),respectively,then decreased to the equivalent level in 24 hours(t=0.360 P=0.731).Compared with air group,COXⅡmRNA expression in hyperoxia group increased significantly at 6 hours(t=2.795 P=0.035),then decreased to the equivalent level at 12 hours(t=0.892 P=0.40) and 24 hours(t=2.018 P=0.071).Conclusions Exposure of hyperoxia up-regulate the expressions of COXⅠmRNA and COXⅡmRNA in AECⅡ,which may be a protective mechanism of hyperoxia-induced lung injury.
9.The application of Herbert screw in the treatment of navicular bone fracture
Wen XIONG ; Hao PAN ; Qiong ZHENG
Chongqing Medicine 2017;46(5):623-625
Objective To investigate the treatment of Navicular bone fracture with Herbert screw.Methods Twenty-three patients with Navicular bone fracture were collected.They were all conducted with CT scan diagnosis and type,the small incision and targeting wire was used in operation.The Herbert screw was used to fix with pressure,plaster was also used to fix.After operation,the activity training was executed gradually.Results We conducted follow-up of 6-18 months in 23 patients,with an average of 12 months.All patients got bone-healing,the healing time ranges from 7 to 10 weeks,with an average of 9 weeks.There was one case of screw penetrating cortical bone,and one case of mild deformity healing,among the other 23 cases there were no complications including infection,nonunion,necrosis,osteoarthritis'etc.Conclusion The treatment of Navicular bone fracture with Herbert screw fixation can get firm fixation,function training and healing early,and it's worth of clinical promotion.
10.Study on the growth and development of fly ( Lucilia Sericata ) under natural temperature and its application on forensic medicine practice
Qingshan NIU ; Yongfeng PAN ; Zhicheng WEN
Chinese Journal of Forensic Medicine 1988;0(04):-
For the purpose of determining the time of death,the life history (the growth and development) and the sum of effective temperature of Lucilia Sericata were studied under the ordinary temperature. On the bases of the growth and development of the fly ,we have established three linear regression equations between the sum of effective temperature and the length of larvae or weigth pupa of Lucilia Sericata (K 1=2 0880+0 8014X 1、K 2=54 0917-2 8814X 2、K 3=133 2180-2 6312X 3).These linear regression equations provide a reliable,scientific method for estimating the time of death in forensic medicine practice.