Objective To explore the diagnostic value of carcino embryonic antigen (CEA),squamous cell carcinoma antigen(SCC),human papilloma virus-E7 (HPV-E7) in cervical carcinoma.Methods A total of 107 cases of women patients treated in hospital from July 2013 to July 2015 accorded to the pathological examination results were divided into cervical cancer group 60 cases and CIN group 47 cases,another 50 cases of healthy people were selected as control group, and serum expression levels of HPV-E7, CEA and SCC in the three groups were detected by enzyme-linked immunosorbent assay.Results The serum HPV-E7, CEA and SCC in cervical cancer group were significantly higher than those in CIN group and control group (P<0.05).There was no significant difference in serum HPV-E7, CEA and SCC between CIN group and control group.The levels of serum HPV-E7, CEA and SCC in stage I-II were significantly lower than those of stage III-IV in patients with cervical cancer, and the difference with statistically significant between two groups(P<0.05).The area under the ROC of HPV-E7 was significantly higher than that of CEA and SCC (Z=2.914,2.951, P<0.05), and there was no significant difference in the area under the ROC between CEA and SCC (Z=1.580,P=0.057).Conclusion The serum HPV-E7, CEA and SCC in cervical cancer patients are significantly higher, the diagnosis value of HPV-E7 is higher which is expected to become one of the effective indicators of cervical cancer diagnosis.

Objective To study the prevalence of chronic kidney disease(CKD) and risk factors among adults received examina-tion in Yongchuan district of Chongqing .Methods 10 280 residents(older than 18 years) were selected ,who had health examina-tion in the Yongchuan Affiliated Hospital ,Chongqing Medical University ,Yongchuan Hospital of Traditional Chinese Medicine and Yongchuan people's hospital from January 2012 to December 2013 .Patients with CKD were identified by the examination dates .All of them were conducted with a questionnaire survey ,physical examination ,and renal damage and related risk indicator factors moni-toring for more than 3 months .Results Eligible dates of 10 021 subjects were enrolled in the study .The prevalence of albuminuria was 5 .1% ,hematuria in 8 .3% ,reduced renal function in 1 .4% .The CKD population prevalence rate was 15 .7% ,and the recogni-tion was 6 .4% .The Logistic regression model showed that hyperuricemia ,nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age contributed to the development of CKD .Conclusion The prevalence of chronic kidney disease is 15 .7% and the recognition is 6 .4% in urban healthy examination population of Yongchuan .Independent risk factors associated with CKD are hyperuricemia , nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age .

Objective To investigate characteristics of emotional intelligence(EI)and the ways of occupational stress coping in nurses as well as the predictive validity of EI on coping strategies.Methods 464 nurses from general hospitals were collected and evaluated with psychometric instruments including:Wong's Emotional Intelligence Scale(WEIS)and Nurse Ways of Coping Questionnaire.Data were conducted by Logistic regress analysis according to key themes.Results (1)Nurses' demographic characteristics had effects to the ways of occupational stress coping and emotional intelligence.However,there was no statistical difference of El varied with education levels(P＞0.05).(2)Both problems solving and positive recognition are main coping styles of nurses towards occupational stress.Either avoidance or self-reproach was seldom coping strategies of nurses toward occupational pressures(2.60±0.55;2.60±0.75;1.37±0.69;respectively).(3)El was correlated with coping styles(r=-0.123～0.253,P＜0.05,P＜0.01).Conclusion Four dimensions of EI positively or negatively predicted six kinds of occupational stress coping strategies separately.

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

Thrombolytic therapy is a safe and effective way to cure thrombosis. Developing the safe,effective and cheap therapeutic thromolytic agents are important to prevent and cure thrombosis. In recent years,several fibrinolytic enzymes have been found in the resources of Asian traditional fermented foods,such as Japanese natto,Korea Chungkook-Jang,Chinese Douchi and fermented shrimp paste. These fibrinolytic enzymes secreted by food-grade microorganisms are safe and effective,and are potent thrombolytic agents. The physical and biochemical properties,thrombolytic characteristics,and the potential applications of these fibrinolytic enzymes from Asian fermented food,are reviewed.

Objective To investigate the correlation between patient' s age and expression of Ki-67 or microvessel density in primary hepatocellular carcinoma.Methods The expression level of Ki-67 and the microvessel density in the hepatocellular carcinoma tissues were detected by immunohistochemical method,and the correlation between age and expression of Ki-67 or microvessel density were analyzed.Results In 48 cases of hepatocellular carcinoma tissues,Ki-67 expressed to varying degrees,but the positive rate was 83.3 ％ (40/45),and there was no expression in corresponding noncancerous tissues (P ＜ 0.01).Microvessel density marked by CD34 in cancer tissues and noncancerous tissues were 32.17±8.06 and 10.83±2.85 respectively.Microvessel density of cancer tissues was higher than noncancerous tissues (P ＜ 0.01).Both levels of Ki-67 expression and microvessel density in hepatocellular carcinoma were negatively correlated with the age (r =-0.418,P =0.003 and r =-0.443,P =0.002 respectively).Conclusion Both levels of Ki-67 expression and microvessel density in hepatocellular carcinoma are negatively correlated with patient' s age,which may suggest worse prognosis for the younger patients.

Objective To study the effect of survivin gene expression on the lymphocyte’s proliferation and function in cultured K562 cells. Methods The constructed recombinant vector pEGFP-C1-survivin and the plasmid pTZU6+1-survivin encoding short hairpin RNA of survivin were transfected into K562 cells respectively to generate K562/survivin+ cells and K562/survivin-cells. K562/survivin+ cells were selected by G418. The survivin mRNA and protein levels in the 3 kinds of cells (K562/survivin+,K562 and K562/survivin-) were detected by semi-quantitative RT-PCR and immunohistochemistry methods. The peripheral blood mononuclear cells (PBMC) from healthy subjects were co-cultured with these 3 cells respectively in mixed lymphocyte-tumor cell culture (MLTC). Lymphocyte proliferation in the supernatant was evaluated by MTT assay. Nature killer activity was detected by flow cytometry and IFN-? level was measured by ELISA assay. Results Proliferation index in K562/survivin+ group was much lower than that in the other 2 groups (P

or =70years of age) with advanced non-small-cell lung cancer. Single agent vinorelbine should be selective chemotherapy in elderly patients with advanced non-small-cell lung cancer.

Objective To investigate the roles of cell apoptosis and the gene expressions of Fas, FasL, bcl-2 and bax in acute rejection of pancreaticoduodenal transplantation and to evaluate the function of duodenum biopsy for early detection of rejection in rats. Methods Wistar and SD rats were divided into two groups: ①Wistar rats that underwent allogenic pancreaticoduodenal transplantation from the organs of SD rats; ②Wistar rats that received homogenic transplantation. The grafts were then harvested on day 3, 5 and 7 after the transplantation, and all graft samples were observed with HE staining and TUNEL was also used to detect apoptotic cells. The expressions of Fas, FasL, bcl-2 and bax were measured by immunochemical method. According to Nakhleh’s score, pathologic features of transplanted pancreas and duodenum were ranged from one to three scores in order. Results The percentage of same or different scores between the pathological scores of pancreas and duodenum in allogenic pancreaticoduodenal transplantation group were 61.1% (11/18) and 38.9% (7/18) respectively, and there were 6 specimens of pancreatic tissue got higher scores with only one higher score for duodenum. There were significant differences of histopathologic rejection scores and apoptotic indices between the two groups, respectively (P