Objective To evaluate the effects of rabeprazole-based triple therapy for associated gastric ulcer caused by the helicobacter pylori(Hp) infection. Methods 100 patients with associated gastric ulcer caused by the Hp infection were randomly divided into treatment group and control group. The treatment group was given rabeprazole-based triple therapy( rabeprazole 10mg bid, amoxicillin 1.0g bid and clarithromycin 500mg bid), and the control group was given omeprazole-based triple therapy( omeprazole 20mg bid, amoxicillin 1.0g bid and clarithromycin 500mg bid). The two groups were given 7 days treatment. Results The total effective rate of clinical symptoms of treatment group was 96. 0% ,the healing rate of the treatment group was 88.0%, the total effective rate of gastroscopy effect of treatment group was 94.0% ,the Hp clearance rate of treatment group was 92.0% and the Hp eradication rate of treatment group was 90. 0% ,which were higher than the control group,but the difference was not significant( P ＞ 0. 05 ). The two groups had no significant adverse reactions. Conclusion Rabeprazole-based triple therapy for associated gastric ulcer caused by the Hp infection was effective, safe and worth popularization and application.

Objective To explore the interventional effect of two different methods in the prevention of chemotherapy phlebitis caused by 5-fluorouracil.Methods Eighty cases of patients treated with cisplatin+5-fluorouracil (PF) chemotherapy in the First Affiliated Hospital of Guangxi Medical University from May 2015 to July 2016 were selected and divided into the observation group and control group according to admission time,40 cases in each group.The observation group was smeared with 2% anisodamine along the direction of vein puncture after peripheral venous puncture success,while the control group was smeared with mucopolysaccharide polysulphate emulsion paste.The occurrence of phlebitis and degree of local pain after intervention were compared between the two groups.The blood biochemical indexes and inflammatory factors levels before and after intervention were compared too.Results After the intervention,the distribution of phlebitis level and local pain degree had statistical difference between the two groups (Z=2.57,2.53;P=0.01);the observation group was superior to the control group in the aspects of phlebitis lesion degree and local pain alleviation;WBC,MPV and PDW in the observation group had statistical difference between before and after intervention;WBC,PLT and PCT in the control group had statistical difference between before and after intervention (P<0.05);PLT count was positively correlated with PCT in the two groups (r=0.952,P<0.05);the CRP level and WBC count after intervention had no statistical difference between the two groups(P>0.05).Conclusion 2% anisodamine can effectively prevent and alleviate the occurrence of 5-FU caused chemotherapy phlebitis and local pain.At the same time,PLT can provide a basis for preventing chemotherapy phlebitis.

Objective To investigate the DNA typing, observe relationship between DNA fingerprinting patterns and serotypes of Cryptococcus neoformans, and find a suitable genotyping standard for Cryptococcus neoformans. Methods Three primers, including CN 1(GTG) 5, CN 2(GACA) 4 and CN 3(GATA) 4, were used to distinguish variations among strains of C.neoformans. Results The distinguishable fingerprinting bands for serotype of C.neoformans were yielded by primer CN 2. Using this primer, of 24 clinical and environmental isolates of serotype A of C.neoformans and 8 standard strains investigated by PCR, 20 strains produced complete identical fingerprinting patterns, the other 4 strains had different fingerprinting patterns. 2 strains of serotype B and C yielded indistinguishable fingerprinting patterns. Conclusions ①The majority of strains of serotype A had similar and stable fingerprinting patterns. ②Some strains of serotype B and C had an indistinguishable fingerprinting patterns. ③The same serotype strains from different sources may produce different DNA fingerprinting patterns. ④The DNA fingerprinting is a rapid, simple and feasible method for identifying Cryptococcus neoformans.

Objective:To observe the efficacy and safety of itraconazole in treatment of onychomycosis and its influence on nail growth speed. Methods: In a multicentre open study involving 15 medical units nationwide, patients with onychomycosis were treated with orally itraconazole pulse therapy. At each stage after treatment, the clinical efficacy,mycological efficacy, and side-effects of the therapy and the net growth length of normal nail deck were observed.Results: The therapy had a satisfactory efficacy and prolonged effect in treating onychomycosis for both affected fingers and toe nails. The therapy was effective for onychomycosis infected with dermatophyton, yeast or non-dermatophyton fungus and had a fungus-eliminating-rate of 97.86%. The average net growth length of normal nail deck in affected toe nails was slightly longer than that in finger nails on 6 months and 9 months after treatment.No severe side effects were found. Conclusion: Itraconazole pulse therapy is effective and safe for onychomychosis and can increase nail growth length,especially for toe nails.

Objective:To study the relationship between susceptibilities of Trichophyton rubrum strains to antifungal agents and their species specificities.Methods: The susceptibilities of Trichophyton rubrum strains to itraconazole,ketoconazole,fluconazole,terbinafine,naftifine,5-flucytosine and amphotericin B were evaluated using a modified microdilution method.The relationship between susceptibilities and genotypes and phenotypes of Trichophyton rubrum strains with different origins was analyzed by random amplified polymorphic DNA(RAPD).Results: The Trichophyton rubrum strains showed narrow minimum inhibitory concentration(MIC) ranges to terbinafine(0.016-0.032 ?g/ml),naftifine(0.032-0.063 ?g/ml) and itraconazole(0.25-1 ?g/ml),whereas they showed broader MIC ranges to ketoconzole(0.25-2 ?g/ml)and fluconazole(1-32 ?g/ml).MICs of Trichophyton rubrum strains to terbinafine(M_0=0.032 ?g/ml) and naftifine(M_0=0.032 ?g/ml) were the lowest among 7 antifungal agents.Wilcoxon test(Kruskal-Wallis test) suggested that there was no significant relationship of MICs to terbinafine,naftifine,itraconazole and amphotericin B with the genotypes,phenotypes and origins of the Trichophyton rubrum strains.Conclusion: The antifungal susceptibility of Trichophyton rubrum strains may not be related to their genotypes,phenotypes or from which part of the body they are isolated.

Objective To investigate the methodology and feasibility of an anatomic survey of fetal limbs in the first trimester. Methods In this study, 533 normal fetus and 6 fetal corpse without limb abnormalities form June 2011 to August 2012 underwent an anatomic survey on the limbs in our consultation center. The limbs were scanned using a systematic continuous sequence approach:(1) Displaying upper arm and humerus, forearms and radius/ulna, hands in proximal to distal direction. (2) The thigh and femur, crus and tibia/fibula, feet was attempted from the sagittal section of each structure. (3) If the whole limb couldn′t be visualized in a section then the three segments will be demonstrated respectively. The position and move-ment also should be observed during the scan. Data were collected regarding gestation age, examining time and the structures. Results The limbs except toes of 533 normal fetus were clearly observed, which was completely unaffected by gestational age, with the mean time of scanning is 98.6±37.4 s (rang:36-189 s). The structures (including upper arms and humerus, forearms ,radius and ulna, hands, thigh and femur, crus and tibia, fibula, feet) of fetal limbs were clearly observed on the 6 fetal corpse, but the ossification center of fingers were steadily visualized until 13 weeks and toes after 13 weeks. Conclusions Scanning fetal limbs in the first trimester were feasible by a systematic continuous sequence approach in a short period of time.

[Objective]To investigate the osteogenic ability in vivo of mesenchymal stem cells(MSCs).[Method]Ten-fifteen ml bone marrow aspirates were harvested from the iliac crest of sheep and enriched for MSCs by density gradient centrifugation over a Percoll cushion(1.073 g/ml).After cultured and proliferated,the tissue-engineered bones were constructed with these cells and seeded onto porous 13-tricalcium phosphate ceramics(13-TCP).Then,the constructs were implanted into left metatarsus defect(21 mm in length)of 8 sheep as an experimental group.Porous ?-TCP composed with bone marrow was implanted into defects of same size and position in 8 sheep as a control group.Sheep were sacrificed in the 6th,12th,and 24th week postoperatively,and the implanted samples were examined by radiograph,histology,and biomechanical analysis.[Result]New bone tissue was observed either radiographically or histologically at the defect area of experimental group as early as the 6th week postoperatively;but was not observed in the control group.Because of the new bone formed in a direct manner without progression through a cartilaginous process intermediately,osteoid tissue,woven bone and lamellar bone,occurred earlier in the experimental group than in the control group,in which the bone defects were repaired in a "creep substitution" manner.At the 24th week,radiographs and biomechanical tests revealed an almost complete repair of the defect in experimental group,but only a partial repair in the control group.[Conclusion]MSCs have good reproductive activity not only in vitro,but also in vivo.The new bone formed in a direct manner without progression through a cartilaginous process intermediately when MSCs were transplanted in vivo.The results demonstrated that MSCs was an excellent seed cells for bone tissue engineering.

To investigate the prognostic value of serum levels of tissue polypeptide specific antigen (TPS) in patients with hepatocellular carcinoma(HCC). Ninety six patients who had undergone hepatoma resections and 22 patients who received radio frequency ablations (RF) were followed up for 6～12 months.The serum levels of TPS were repeatedly determined.The RF group patients were divided into three subgroups: improved ,stable,and advanced.The median levels (U/L) of serum TPS in advanced subgroup were always higher than those in improved subgroup ( P

Objective To study the expression of Notch 1,Jagged1 and Notch intracellular domain (NICD) in epithelial ovarian carcinoma tissues and analyze the clinical significance.To explore the activity of γ-secretase in epithelial ovarian carcinoma cell line SKOV3 and the effect of N-[N-(3,5-dil uorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT),a γ-secretase inhibitor on the activity of γ-secretase in SKOV3.Methods Immunohistochemistry staining method was performed in 43 patients with epithelial ovarian carcinoma and 11 patients with benign epithelial ovarian tumor to detect the expression of Notch1,Jagged1 and NICD.The differences of expressionof Notch1,Jagged1 and NICD between malignant and benign ovarian tumors was compared and alsoanalyzed the correlation with clinicopathological parameters of ovarian carcinoma.Human serous ovarian cancer cell line SKOV3 and immortalized nontumorigenic ovarian epithelial cell line T29 were incubated in vitro.The activities of γ-secretase in SKOV3 and T29 with dimethyl sulfoxide (DMSO) and DAPT were detected respectively by Gal4VP16/UAS and dual luciferase reporter assay system.Results (1) The immunohistochemical composite scores (ICS) of Notch1 in epithelial ovarian carcinoma (6.7±2.2) were not significantly different with those in benign epithelial ovarian tumor (5.4± 2.7,P=0.153),while the ICS of Jagged 1 and NICD in epithelial ovarian carcinoma (5.3± 2.4,5.3± 2.3) were higher than those in benign epithelial ovarian tumor (1.6± 1.4,3.1± 1.7; all P＜0.01).The expression of Notch 1,Jagged 1 and N ICD had no correlation with patients' aged,history of carcinoma,ascites,the level of serum CA125,maximum length of ovarian tumor,Federation International of Gynecology and Obstetrics (FIGO) stage,grade and pathology subtypes (all P＞0.05).The hazard ratio between the high expression of Notch1,Jagged1,or NICD and the moderate to low expression of Notch1,Jagged1,or NICD,and Jagged1 were 0.771,1.648 and 1.316,respectively (all P＞0.05).The 5-year survival rate and median survival time between the high expression of Notch,Jagged 1 or NICD in subgroup and moderate to low expression in subgroup were of no difference (all P＞0.05).The activity of γ-secretase in SKOV3 was significantly higher than that in T29 [(12.2± 1.4)％,P=0.019].(2)After DAPT treated,the relative activity of γ-secretase in SKOV3 (50 μmol/L) was declined from (100.0±5.3)％ to (6.6±0.8)％ (P=0.001).Conclusions Jagged1 and NICD in Notch1 pathway may play a key role in the occurrence of ovarian carcinoma.The activity of γ-secretase in epithelial ovarian carcinoma was higher than that in ovarian epithelial cell which suggest that DAPT,γ-secretase inhibitor,may become the target of ovarian carcinoma treatment.

OBJECTIVE:To evaluate the status quo and the prospect of trazodo ne in the treatment of sexual dysfunction.METHODS:Data concerning the clinical applications of trazodone in the treatment of sexual dysfunction from the Chinese biomedicine bibliographic database,the database of Qinghua tongfang and the Huipu database during the period of1997～2004were referred to on the internet and which were analyzed statistically.RESULTS:There were17of which were research papers on erectile dysfunction and prospermia and7of which were review articles,the total cases for treatment were910,the average effective rates were71%.CONCLUSION:It deserves attention for the medicine circle to give a further study and research on the effects of trazodone in the treatment of male sexual dysfunction,especially the prospermia.