Aim To observe the effect of DL-buthionine s ulfoximine(DL-BSO) on the radiosensitivity of rat C 6 glioma cells under the a erobic and the hypoxic condition. Methods The source of radiati on was 60Co ?-rays. The rats C 6 glioma cells were treated by radia tion alone or DL-BSO+radiation under the aerobic and the hypoxic condition. Col ony forming assay was used to measure effects of DL-BSO on the radiosensitivity . Results Radiosensitive effect of DL-BSO was time-depedent u nder the aerobic condition. After treatment with 0.1 mmol?L -1 DL-BSO fo r 2, 6, 12 hours, the radiosensitive effect was not observed, whereas an enhance ment of radiosensitivity was seen at 24 and 48 hours. An enhancement of radiosen sitivity was seen at 2～48 hours after treatment with 0.1 mmol?L -1 DL-B SO under the hypoxic condition. The radiosensitive effects related to DL-BSO co ncentration under the aerobic and the hypoxic condition. Conclusion Both under the aerobic and the hypoxic conditions DL-BSO can increase the radio sensitivity of rat C 6 glioma cells. DL-BSO increased the rat C 6 gliom a cells radiosensitivity especially under the hypoxic condition, and radiosensit ive effect of DL-BSO is time and concentration-dependent.

Objective To study the effect of nifedipi ne ,an endothelin antagonist,in acute renal function impairment caused by ESWL. Methods 60 cases of renal stone underwent anaesthesia-free ESWL were radomely assigned into 2 groups,the nifedipine group and the control (n=30 each).In the former group,nifedipine was given 3 days before ESWL.Plas ma endothelin,urinary ? 2-microglobulin,and urinary Tamm-Horsfall protein we re assessed to evaluate the renal function. Results In t he controls,it was a significant increase of plasma endothelin and urinary ? 2-MG(P

ObjectiveTo evaluate wedge resection of th e back labium renalis in the repairation of iatrogenic pelvis tear. MethodsFrom October 1999 to February 2002,7 cases of iatrogenic pel vis tear were repaired with wedge resection of the back labium of renal hilum.Th ere were 5 male patients and 2 female with an average age of 45.The tear occurre d during lithotomy for pelvis stone in 6 and transureteroscopic lithotripsy in 1.ResultsThe mean operative time was 130 min and the m ean blood loss 300 ml,postoperative IVU showed no obstruction of the renal pelvi s,and the renal function has been normal.All patients have been followed up for 3 months to 3 years.Neither hydronephrosis nor renal pelvis obstruction has been noted.ConclusionsThe procedure has the advantages of no need of predicle occlusion,less bleeding,a clear operation field,easy repaira tion,and the avoidance of pelvis obstruction.

To evaluate the feasibility of using polyethyleneimine (PEI) coated magnetic iron.oxide nanoparticles (polyMAG-1000) as gene vectors. The surface characteristics of the nanoparticles were observed with scanning electron microscopy. The ability of the nanoparticles to combine with and protect DNA was investigated at different PH values after polyMAG-1000 and DNA were combined in different ratios. The nanoparticles were tested as gene vectors with in vitro transfection models. Under the scanning electron microscope the nanoparticles were about 100 nm in diameter. The nanoparticles could bind and condense DNA under acid, neutral and alkaline conditions, and they could transfer genes into cells and express green fluorescent proteins (GFP). The transfection efficiency was highest (51%) when the ratio of nanoparticles to DNA was 1:1 (v:w). In that ratio, the difference in transfection efficiency was marked depending on whether a magnetic field was present or not: about 10% when it was absent but 51% when it was present. The magnetic iron oxide nanoparticles coated with PEI may potentially be used as gene vectors.
Breast Neoplasms/metabolism ; Breast Neoplasms/*pathology ; Cell Line, Tumor ; Ferric Compounds/*chemistry ; Ferric Compounds/metabolism ; Gene Targeting ; Genetic Vectors ; Green Fluorescent Proteins ; Magnetics ; Nanotechnology ; Particle Size ; Polyethyleneimine/chemistry ; Transfection/methods

The aim of this study was to evaluate the feasibility and efficacy of using TRAIL gene to treat breast cancer mediated with a novel carrier - magnetic iron oxide nanoparticles (poly-MAG-1000) coated with PEI. The magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into the tumor cells as negative control. TRAIL gene transfection with liposome as carrier served as positive control. The apoptosis of cells was detected with TUNEL method. The apoptosis ratio of tumor cells was measured with flow cytometry (FCM). It was found that the apoptosis occurred in the tumor cells after transfection of TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMAG-1000 as gene carrier was (25.11+/-2.85) %, whereas it was (5.06+/- 1.05) % in the control group with polyMAG-1000 (P<0.01). The apoptosis ratio was as low as (18.31+/-2.44) % in the group with liposome as gene carrier (P<0.05, as compared with the group with polyMAG-1000 as gene carrier). It is suggested that TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The magnetic iron oxide nanoparticles coated with PEI may be a potential gene carrier with high transfection efficacy for cancer gene therapy..

Objective:To evaluate the feasibility of using polyethyleneimine(PEI) coated magnetic iron oxide nanoparticles(polyMAG-1000) as gene vectors. Methods:The surface characteristies of the nanoparticles were observed with scan electronical microscope.The ability of the nanoparticles to combine and protect DNA were investigated at different PH after the polyMAG-1000 and DNA were combined at different ratio.The nanoparticles were tested as a gene vectors through transfection models in vitro. Results: Under scan electronical microscope, the diameter of the nanoparticles was about 100 nm. The nanoparticles could bind and condense DNA under acidic ,neutral and alkaline pH conditions. The nanoparticles could transfer gene into cell and express green fluorescent protein(GFP).The efficiency of transfection was the highest when the ratio of the nanoparticles and DNA was 1 ∶ 1(v ∶ w).The difference was marked in the transfection efficiency when magnetic field was added or not. Conclusion: The magnetic iron oxide nanoparticles coated with PEI may be potentially used as gene vectors.

Objective To evaluate wedge resection of the back labium renalis and intrasinusal pyelolithotomy for staghorn calculi of kidney in patients with intrarenal pelvis and renal malrotation. Methods From August 1998 to February 2001, 18 cases of staghorn stones of kidney were removed via the wedge resection of the back labium of hilum renalis and incision of the intrarenal sinus. Results The mean operative time was 110 min and the mean blood loss 300 ml.The biggest stone was 6.5 cm?5.0 cm?3.0 cm and in one case the number of stones amounted to 1 400. Postoperative KUB showed no residual stones and postoperative IVU indicated normal renal function.All patients have been followed up for 1～3 years and have been free of stone. Conclusions The procedure has the advantages of no need of pedicle renalis occlusion,less bleeding,a clear operation field,easy staghorn calculi removal and the avoidance of renal hilum outlet obstruction.

Objective To study the early diagnostic value of CT for children with cerebral palsy.Methods CT manifestations of 124 cases with cerebral palsy were analysed retrospectively. Results 96 cases of the 124 cases showed a recognisable abnormality,the abnormal rate of CT was about 77.42％ ,and the most common abnormality was cerebral atrophy.Spastic type was the most common clinical type(71.77％ ).The less of the age,the higher of the abnormal rate of CT. Conclusion Although CT isn't the main basis of the diagnosis of cerebral palsy,it's helpful for us to find the pathological changes,and to find the eliology and location.It also provided basis for us to judge the prognosis of cerebral palsy.So CT has significant value in the early diagnosis of cerebral palsy.

Objective To study and evaluate the feasibility and clinical effects of the treatment of premature ejaculation with modified Masters-Johnson sensate focus exercise.Methods 100 male outpatients with premature ejaculation were divided randomly into two equal groups randomly.The experiment group was treated with the modified Masters-Johnson sensate focus exercise and oral sertraline 50 mg/day for 8 weeks.The control group was given oral sertraline 50 mg/day for 8 weeks.The scores were evaluated with CIPE-5 and compared between the groups before and after the treatment and after the flown-up for 6 moths.Results After treatment,the score of CIPE-5 were better in experiment group than that in control group (P＜0.01).The effective rate 92％ (46/50) in experiment group was significantly higher than that in control group 68％ (34/50) (P＜0.01).The relapse cases (6 cases) were significantly lower than that in the control group (22 cases) (P＜0.01).Conclusions The method of Masters-Johnson sensate focus exercise for treatment of premature ejaculation has a good curative effect,and low recurrence rate,which is worth the clinical promotion.

Objective To introduce and analyze the current user security authentication system in the campus network and point out the secure problems and hidden dangers of the correspondence entities in the campus network. Methods The authentication technologies and protocols in the TCP/IP network model were compared. It was proposed that the campus network security authentication solution should include access control in data link layer, connection control in network layer and unified authentication in application layer. Results The solution could improve the campus network authentication security rank. Conclusion The security and reliability of the network system are enhanced.