Objective:To study the correlation between the effects of oxygen free radicals (OFR) with T cell subsets on pathogenesis of hemorrhagic fever with syndrome(HFRS).Methods:Serum levels of SOD and MDA.T cell subsets in 54 patients with HFRS were sequentially investigate and correlation. Results:The level of serum SOD in febrile phase was lower than that of normal control.The level of serum MDA in febrile phase was higher than that of normal group;the sum of CD4 + cell in febrile phase was signficantly lower than that normal group,CD8 + cell was significantly higher than that of control group,and its changes must severe in oliguric phase.The resuts also showed that there was sum correlation existed between MDA and T cell subsets.Conclusion:These findings demonstrated that irregulating immunity of HFRS patients was correlation with OFR.

Objective: To explore the mechanism of Xuebijing injection in the treatment of acute paraquat poisoning by means of studying the expression of TNF-alpha, NF-kappa B, Caspase-3 and the changes of cell apoptosis rate detected by TUNEL in the lung tissue of acute paraquat-induced rats. Methods: On the base of random number table, 126 Wister rats weighing 220 g to 270 g were divided into 3 groups: (1) Control group: 42 rats, (2) Poisoned group: 42 rats, (3) Treatment group: 42 rats. On 1^st、3^rd、7^th、14^th、21^st、28^th、and 35^th day, six rats from each group were anaesthetized by intraperitoneal injection of chloral hydrate. To cut the chest and take the lung tissue samples. The expression levels of Tumor Necrosis Factor-alpha, Nuclear Factor-kappa B and Caspase-3 protein in lung tissue were detected by immunohistochemical staining, as well as apoptotic cell rate was detected by TUNEL staining. Results: The expression levels of Tumor Necrosis Factor-alpha, Nuclear Factor-kappa B, Caspase-3 protein and TUNEL staining in the lung tissue of the poisoned group was significantly higher than that of the control group (P<0.05) . Compared with the poisoned group, the expression of TNF-alpha, NF-kappa B, Caspase-3 and TUNEL in treatment group decreased significantly (P<0.05) , but they were still higher than those of the control group, and the difference was statistically significant compared with the control group (P<0.05) . Conclusion Apoptosis and TNF-alpha, NF-kappa B and Caspase-3 play an important role in lung injury of paraquat-induced rats. Xuebijing injection can inhibit the expression of TNF-alpha, NF-kappa B, Caspase-3 in lung tissue, reduce the apoptosis rate and alleviate the damage of lung tissue in paraquat-poisoning rats.