1.Influence of anal sphincterotomy to anorectal dynamics in the treatment of hemorrhoids
Chanyu LI ; Yu GU ; Shusen LIN ; Min NIE ; Hao YU ; Lu YUAN ; Weiyu GUAN ; Peng YUAN ; Chengfei SONG ; Mofei WANG ; Jun WANG
Chinese Journal of Postgraduates of Medicine 2009;32(26):23-25
Objective To observe the difference of anoreetal dynamics between Milligan-Morgan hemorrhoidectomy plus internal sphinctemtomy and simple Milligan-Morgan bemorrhoidectomy in the treatment of annulus mixed hemorrhoids.Method Measured the anal resting pressure,maximal anal contractive pressure,rectal sensation thresholds,maximal rectal tolerable dose and maximal rectal compliance 1 day before and 3 months after Milligan-Morgan hemorrhoidectomy plus internal sphincterotomy (therapy group,50 cases)and simple Milligan-Morgan bemorrhoidectomy(control group,52 cases)by anorectal manometric device made in Sweden.Results The anal resting pressures of therapy group and eontrol group reduced signifieanfly 3 months after operation compared with that 1 day before operation(P< 0.01 or < 0.05),but there was significant difference between the two groups in 3 months after operation(P<0.05).The maximal anal contractive pressure,reetal sensation thresholds,maximal rectal tolerable dose and maximal rectal compliance were no significant difference between the two groups in 3 months after operation (P >0.05).Conclusion Anal sphineterotomy can change the high anal pressure significantly in the treatment of annulus hemorrhoids without copracrasia,it is a proper operation method.
2.Role of uropathogenic Escherichia coli virulence factor TcpC in immune evasion and its pathogenic mechanism
Weiyu JIANG ; Jiaying FAN ; Liming FAN ; Jiadong WANG ; Ziyan JIANG ; Ziyu GUAN ; Qian OU ; Jiaqi FANG
Chinese Journal of Microbiology and Immunology 2024;44(3):198-204
Objective:To investigate the role of TcpC, a virulence factor of uropathogenic Escherichia coli (UPEC), in immune evasion, and analyze its related pathogenic mechanism. Methods:C57BL/6 mice were injected with 10 9 colony-forming unit of wild-type (CFT073 wt) or tcpc gene-knockout (CFT073 Δ tcpc) UPEC CFT073 strains from urethra into bladder to construct a mouse model of pyelonephritis. These mice were sacrificed 5 d after infection and their kidneys were taken to observe the gross pathological changes. Hematoxylin-eosin staining was used to observe histopathological changes in kidney tissues and immunohistochemistry was performed to locate TcpC in kidney tissues. The bacterial loads in urine samples of UPEC infected-mice were counted by ten-fold dilution method, and the presence of tcpc gene in the genomic DNA of bacteria from CFT073-infected mouse kidney or urine samples was measured by PCR. The expression of TcpC at mRNA level was detected by qRT-PCR after infecting dendritic cells with CFT073 wt strains. The influences of UPEC infection on the activation of NF-κB signaling pathway and the secretion of proinflammatory factors by dendritic cells were analyzed by Western blot and ELISA, respectively. The viability of UPEC strains in dendritic cells were observed by laser confocal microscope. Results:Compared with the CFT073 Δ tcpc group, the mice in the CFT073 wt group had obvious abscess in the kidneys as well as massive neutrophil infiltration and abundant TcpC in kidney tissues. The bacterial loads in the urine of CFT073 wt-infected mice were significantly higher than those in the urine of CFT073 Δ tcpc mice. PCR results showed that tcpc gene was successfully amplified from mouse kidney and urine samples. Increased expression of TcpC at both mRNA and protein levels was detected in CFT073 wt-infected dendritic cells. CFT073 wt infection inhibited the phosphorylation of NF-κB p50 and the production of proinflammatory factors in dendritic cells. TcpC promoted the survival of CFT073 wt in dendritic cells. Conclusions:TcpC expression increases significantly during CFT073 wt infection or in mice with CFT073 wt-induced pyelonephritis. It promotes the survival of CFT073 wt in dendritic cells by inhibiting the activation of NF-κB signaling pathway and reducing the secretion of pro-inflammatory cytokines. TcpC is involved in the pathogenesis of UPEC and immune evasion.
3.Mechanism of action and potential value of the IRE1α/TRAF2/JNK pathway in the progression of acute liver failure
Haimei GUAN ; Kan ZHANG ; Weiyu CHEN ; Guobao LI ; Yangling ZENG ; Riyun ZHANG ; Tianwen WANG ; Baohua XIE ; Dewen MAO
Journal of Clinical Hepatology 2024;40(6):1281-1288
Acute liver failure (ALF) is one of the most critical liver diseases in clinical practice and seriously affects the life and health of Chinese people. Due to its high morbidity and mortality rates, unclear pathogenesis, and limited treatment methods, ALF has become a major problem that needs to be solved urgently in the field of liver diseases. In recent years, more and more studies have shown that endoplasmic reticulum stress is a key biological process in the progression of ALF, and the IRE1α/TRAF2/JNK pathway, as a part of endoplasmic reticulum stress signaling, plays a role in amplifying inflammatory response, promoting hepatocyte apoptosis, and inhibiting liver regeneration ability during the progression of diseases. As a traditional treasure of China, traditional Chinese medicine has become a research hotspot in search for effective prevention and treatment drugs for ALF from monomers of Chinese herbs. This article elaborates on the mechanism of action of the IRE1α/TRAF2/JNK pathway in the progression of ALF and summarizes the potential value of several monomers of Chinese herbs in regulating this pathway, such as salidroside, Fructus Broussonetiae, Fructus Psoraleae+Schisandra chinensis, baicalein, genipin, kaempferol, resveratrol, sea buckthorn polysaccharide extract, and luteol, in order to provide a reference for further research and clinical practice of ALF.