1.Aplastic anemia and clonal evolution:germline and somatic genetics
Journal of Leukemia & Lymphoma 2017;26(3):138-141
Clonal progression to myelodysplastic syndromes (MDS) or acute myeloid leukemia (AML) remains a dreaded complication for a subset of patients with bone marrow failure (BMF). Recognizing risk factors for the development of MDS or AML would inform individualized treatment decisions and identify patients who may benefit from early or upfront hematopoietic stem cell transplantation. Now that next-generation DNA sequencing is available in the clinical laboratory, research has focused on the implications of germline and somatic mutations for diagnosing and monitoring patients with BMF. Most germline genetic BMF disorders are characterized by a high propensity to develop MDS or AML. Recent studies of somatic variants in marrow failure revealed a high frequency of clonal hematopoiesis with the acquisition of mutations in genes associated with MDS or AML. The evaluation and implications of germline and somatic mutations for the development of clonal disorders in patients with BMF and challenges of clinical genetic testing will be summarized in this paper based on the reports from the 58th American Society of Hematology (ASH) Annual Meeting.
2.Detection of IL-1? and PGE_2 in periapical exudates in the patients with periapical periodontitis and their correlations with clinical findings
Weihong LIU ; Jinhua YU ; Weiyi YU
Journal of Practical Stomatology 2001;0(03):-
Objective: ①To detect the concentrations of interleukin 1?(IL 1?) and prostaglandin E 2 (PGE 2) in periapical exudates in the patients with acute or chronic periapical periodontitis; ② To examine their correlations with clinical and radiographic findings of the involved teeth.Methods: The volume of periapical exudates was quantified using the standard paper point sampling method; the concentration of IL 1? and PGE 2 was detected using enzyme linked immunosorbent assay(ELISA) or radioimmunoassay(RIA) in 40 cases with acute and in 40 with chronic periapical periodentitis. Results: ①The volume(?l) of the exudates in the cases with acute periapical periodontitis and chronic were 27.10?15.70 and 3.12?2.88( P
3.THE DYNAMIC CHANGE IN ICE mRNA EXPRESSION AND ITS EFFECT ON IFN-? IN THERMAL INJURED RATS
Jingqu LIU ; Weiyi GAO ; Yongmin YAO
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
To investigate the dynamic changes in ICE mRNA expression and its relationship with IL 18 and IFN ? mRNA expression in thermal injured rats.The animals were subjected to a 30% total body surface area full thickness thermal injury,and fluid resuscitation was performed at 6 hours post burn. 54 male Wistar rats were randomly divided into three groups: normal controls,30% Ⅲ degree burn group,and selective decontamination of the digestive tract (SDD) treatment group. P1asma endotoxin was measured by limulus amebocyte lysate test,and tissue ICE,IL 18 and IFN ? mRNA expression was determined by reverse transcription po1ymerase chain reaction (RT PCR).The results showed that ICE, IL 18, and IFN ? mRNA expression significantly increased in the intestine,1ung,liver,and kidney at 2 hours,peaking at 8 hours,and a high level was maintained till 24 hours. However, treatment with SDD could lower plasma endotoxin levels,and markedly inhibit ICE, IL 18, and IFN ? mRNA expression in various organs.This study suggests that major burns can lead to a marked elevation of tissue ICE mRNA expression in vital organs,which might be associated with IL 18 and IFN ?mRNA expression induced by gut derived endotoxemia.
4.Grape seed procyanidin extract arrests cell cycle of human bladder cancer cell line BIU87
Jie LIU ; Qianyuan ZHUANG ; Weiyi ZHANG
Journal of Third Military Medical University 2003;0(17):-
Objective To study the effect of grape seed procyanidin extract(GSPE) on the cell cycle arrest in human bladder cancer BIU87 cells and investigate the underlying molecular mechanism.Methods BIU87 cells were treated with different concentrations of GSPE(50,100 and 200 ?g/ml) and cultured for 24 h.The untreated group served as control.Flow cytometry was used to evaluate the cell cycle,and RT-PCR and Western blotting were used to detect the mRNA and protein expressions of CyclinD1 and cyclin-dependent kinase 4(CDK4).Results GSPE inhibited the cell growth through cell cycle arrest at G1 phrase in a dose-dependent manner.Semi-quantitated RT-PCR and Western blot analyses indicated that GSPE decreased CyclinD1 and CDK4 expressions in a dose-dependent fashion(P
5.The CorreIation of Serum BiIirubin and Hearing Loss of Neonate with Hypercholesterolemia
Sumin JIANG ; Shiiin LIU ; Li ZHANG ; Weiyi LIU ; Xuemei JIAO
Journal of Audiology and Speech Pathology 2015;(1):37-39
Objective To study the relationship between hearing loss and serum bilirubin of neonates with hypercholesterolemia .Methods The serum bilirubin level and auditory brainstem response thresholds of 70 hyper_cholesterolemia neonates of full term pregnancy and normal weight were tested ,and the correlations of serum biliru_bin ,and occurrence time of jaundice with hearing threshold were analyzed .ResuIts Among the 70 neonates ,there were 17 cases with normal ABR thresholds and 53 with abnormal ABR thresholds ,including 27 with middle and se_rious hearing loss .In the cases of serum bilirubin less than 342μmol/L ,the percentage of cases with middle and se_rious hearing loss and with occurrence time of jaundice less than 48 hours was higher than theses cases with occur_rence time of jaundice more than 48 hours (P<0 .05) .In the cases of serum bilirubin higher than 342μmol/L ,there was no obvious difference between the percentage of cases of middle and serious hearing loss with occurrence time of jaundice less than 48 hours and those with occurrence time of jaundice more than 48 hours .Among the 53 cases with abnormal hearing thresholds ,positive correlation was observed between serum bilirubin and ABR threshold (r=0 .041) ,whereas negative correlation was observed between occurrence time of jaundice and ABR threshold (r=-0 .291) .ConcIusion The higher probability of hearing loss for hypercholesterolemia neonates with earlier occur_rence of jaundice ,the more serious hearing loss with higher serum bilirubin .
6.Association between polymorphism of leptin receptor gene and serum leptin in asthma
Wei LIU ; Xia JI ; Weizhong ZHANG ; Fenghua WANG ; Weiyi ZHANG
Clinical Medicine of China 2013;29(11):1147-1150
Objective To investigate the relationship between leptin receptor(LEPR) gene Gln223Arg in polymorphism and serum leptin level of patients with asthma.Methods A case-control study was conducted.One hundred and ninety-two asthma patients were recruited from asthma gene library,among them 100 patients only with asthma(asthma group) and 92 patients with both asthma and metabolic syndrome(asthma complicated with metabolic syndrome group).And 108 normal people were selected as control group.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of LEPR gene Gln223Arg locus.Serum leptin levels were measured by enzyme-linked immuno sorbent assay,its relationship with asthma was analyze.Results The frequencies of genotype GG,GA,AA in asthma group,asthma complicated with metabolic syndrome group and normal control group were (83.00% (83/100) vs.83.70% (77/92) vs.70.37% (76/108),15.00% (15/100) vs.15.22% (14/92) vs.27.78% (30/108),and2.00% (2/100) vs.1.08% (1/92) vs.1.85% (2/108) respectively).G and A alleles in asthma group,asthma complicated with metabolic syndrome group and normal control group were 90.50% vs.91.30%vs.84.26%,9.50% vs.8.70% vs.15.74% respectively.Compared with the control group,GG genotype and GA + AA genotype were different between in asthma group and asthma complicated with metabolic syndrome group.The patients with GG genotype showed 2.056 (x2 =4.599,P =0.032,OR =2.056,95% CI =1.057-3.999) and 2.161 folds (x2 =4.907,P =0.027,OR =2.161,95 % CI =1.084-4.311) risk in asthma group and asthma complicated with metabolic syndrome group than that with GA + AA genotype.In terms of leptin levels,in asthma group,female were significant higher than that of control group (P =0.037,P =2.93),while in asthma complicated with metabolic syndrome group,male and female were significantly higher than control group (P =0.001,0.000 ; P =4.530,4.690).Conclusion The polymorphisms of LEPR gene locus Gln223 Arg are related with asthma.Genotype GG carrier is susceptible for the disease.In female,serum leptin is positive relationship with asthma group,especially in asthma complicated with metabolic syndrome.
7.Generation and application of three-dimensional cell traction force
Chen LIU ; Weiyi CHEN ; Xiaona LI ; Xiaojun WANG
Chinese Journal of Tissue Engineering Research 2017;21(8):1301-1305
BACKGROUND: Traction force generated by adherent cells exerts on the extracellular matrix or cell substrate through the interaction between actin and myosin, which involves a variety of physiological and pathological processes.OBJECTIVE: To review the fundamental principles and application of several traction force techniques.METHODS: The first author retrieved the databases of CNKI, WanFang, PubMed and SpringerLink for the research addressing the measurements of traction force using the keywords of cell traction force, biomechanics in Chinese and English, respectively. Repetitive articles were excluded, and 30 literatures were enrolled for analysis, including 1 Chinese and 29 English articles.RESULTS AND CONCLUSION: There are three kinds of measurements of traction force, such as silica gel membrane, traction force microscopy, and micromachined levers. The precise measurement of traction force contributes to further understand the cell-cell and cell-microenvironment mechanical interactions.
8.Study of platelet aggregations in patients with coronary heart disease and the effect of ticlopidine treatment
Yujie LI ; Weiyi MAI ; Qunying ZENG ; Qing HE ; Lili LIU
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To investigate the platelet aggregations in patients with coronary heart disease(CHD) and the effect of ticlopidine treatment. METHODS: Platelet aggregations induced by adenosine diphosphate(ADP), epinephrine(EPI), collagen(Coll), arachidonic acid(ACA) in CHD group before and after ticlopidine treatment were measured by turbidity assay. RESULTS: Maximum ratios of platelet aggregations (max%) induced by ADP,EPI in CHD group (0.78?0.23,0.86?0.25) were significantly higher than that of control group (0.65?0.19,0.73?0.21, P
9.Pulsed radiofrequency and radiofrequency thermocoagulation in the treatment of idiopathic infraorbital nenralgia
Weiyi GONG ; Gang LIU ; Baoguo WANG ; Hailong JIN
Clinical Medicine of China 2009;25(4):392-395
Objective To study the effects of pulsed radiofrequency(PRF)and radiofrequency thermocoagulation(RFTC)in the treatment of idiopathic infraorbital neuralgia.Methods 16 patients suffering idiopathic infraorbital neuralgia were randomly divided into PRF(n=8)and RFTC(n=8).Numeral rating scale(NRS),pain relief(PR)and effective rate of treatment was performed before operation and at 1 hour、1 st day、7th day、15th day、1 st month、3rd month and 6th month after operation.Results The preoperative and postoperative impedance of thefirst,second and third operation in RFTC group were(490±86)Ω and(424±82)Ω,(492±90)Ω and(432±84)Ω,(481±98)Ω and(409±85)Ω respectively and the postoperative impedance was significantly decreased(P<0.05).The preoperative NRS in group PRF and RFTC were 9(7~10)and 8.5(7~10)respectively and the postoperative NRS at all points in two groups were significantly decreased as compared with the preoperative NRS(P<0.05).The PR at 7 and 15d in group PRF were 3(0~4)and that in group RFFC were 4(3~4)(P<0.05).There were no significant differences in effective rate of treatment at each time between two groups(P>0.05).Numbness appeared after operation in group PRF and disappeared in a week,however,that was persistent and all sense disappeared in group RFTC.Conclusion Curative effect of PRF for idiopathic infraorbital neuralgia is as well as RFTC and it is a kind of safe and effective technology that causes less side effects.
10.Determination of berberine hydrochloride in Fuyanping capsules by HPLC
Ying ZHAI ; Xu CHEN ; Weiyi LIU ; Yikun SUN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(11):-
Objective:To establish the quality standard of berberine hydrochloride in Fuyanping capsules by HPLC. Methods:HPLC with Phenomenex C18(250?4.6mm,5?m)column was used.Acetonitrile-0.033mol/l potassium dihydrogen phosphate(30:70)was used as a mobile phase,flow rate was 1.0ml/min and detection wavelength was set at 346nm.Results: Berberine hydrochloride showed a good linear relationship at a range of 0.16-0.83?g,r=0.9999.The average recovery was 99.09%,and RSD was 0.20%.Conclusion:This method is simple,fast and accurate for the quality control of Fuyanping capsules.