Objective:To investigate whether CD3McAb activated killer(CD3AK)cells could induce apoptosis in HL 60 cells in serum free culture system.Methods:HL 60 cells were coincubated with CD3AK cells expanded in serum free medium,then apoptosis was assessed by means of morphological studies,DNA agarose gel electrophoresis and flow cytomether(FCM) analysis.Results:After 7 h of coincubation with CD3AK cells in serum free medium,HL 60 cells showed morphological features of apoptosis and typical DNA ladder on gel electrophoresis.FCM analysis showed that 24.59% cells were found to undergo apoptosis.Conclusion:CD3AK cells can induce apoptosis in HL 60 cells under serum free culture conditions.

Objective To investigate the relationship between donor liver warm ischemia and postoperative cytokine expression in liver transplantation from non-heart-beating donors.Methods Rat orthotopic liver transplantation was performed in three groups (HB, NHBD-30 and NHBD-60) according to the non-heart-beating time of 0, 30 or 60 minutes before donor liver was harvested. The serum values of TNF-?, IL-6 and CINC in each group after transplantation were determined.Results Over time of warm ischemia to which the donor liver was exposed, the concentrations of TNF-?, IL-6 and CINC elevated with significant difference among those three groups. TNF-?, IL-6 and CINC reached its peak value at 3 hours, 6 hours and 6 hours after transplantation respectively.ConclusionsKG1 In liver transplantation from non-heart-beating donors, the upregulation of TNF-?, IL-6 and CINC was associated with warm ischemia injury.