Objective To investigate the early diagnosis and disease evaluation value in patients with acute pancreatitis by various serum cytokines measurement.Methods Forty-eight acute pancreatitis patients were divided into two groups based on the results of computed tomography (CT) examination:mild acute pancreatitis group (30 cases) and severe acute pancreatitis group (18 cases).The other 30 normal persons were selected as control group.The various serum cytokines were measured by enzyme-linked immunosorbent assay (ELISA).Results The serum concentrations of interleukin(IL)-1,IL-10 and tumor necrosis factor (TNF)-α in mild acute pancreatitis group were significantly higher than those in control group [(25.00 ± 1.92) ng/L vs.(10.08 ± 2.65) ng/L,(59.78 ± 4.51) ng/L vs.(1.80 ± 0.66) ng/L,(55.31 ± 8.54) ng/L vs.(18.72 ± 7.84) ng/L,P ＜ 0.05].The serum concentrations of IL-1,IL-6,IL-8,TNF-α and platelet activating factor (PAF) in severe acute pancreatitis group were significantly higher than those in mild acute pancreatitis group [(93.27 ± 3.98) ng/L vs.(25.00 ± 1.92) ng/L,(397.84 ± 13.05) ng/L vs.(34.12 ± 4.96) ng/L,(93.32 ±3.40) ng/Lvs.(13.06± 1.86) ng/L,(181.94 ±7.54) ng/Lvs.(55.31 ±8.54) ng/L,(284.53 ±7.88) ng/L vs.(175.25 ±30.15) ng/L,P＜0.05].Conclusion The various serum cytokines measurement has great importance on the early diagnosis of acute pancreatitis and discrimination between the mild acute pancreatitis and severe acute pancreatitis.

ObjectiveTo observe the therapeutic efficacy of warm needling plus acupoint injection in treating post-stroke urinary retention.MethodSeventy eligible subjects with post-stroke urinary retention were randomized into a treatment group of 35 cases and a control group of 35 cases. The treatment group was intervened by warm needling plus acupoint injection, while the control group was by warm needling alone. The relevant symptoms of urinary retention were observed before and after treatment.Result After 1-week treatment, the retention condition was improved in both groups, and the treatment group was significantly superior to the control group (P<0.05); The 2-week and 1-month follow-up studies showed that the retention condition in the treatment group was significantly better than that in the control group (P<0.05). The adverse events in the treatment group were significantly less than that in thecontrol group (P<0.05).ConclusionWarm needling plus acupoint injection can produce a satisfactory efficacy in treating post-stroke urinary retention and is worth application in clinic. However, this method still needs improving as its effect on initialurination and urination duration is not significant.

Objective: To investigate the influence of lidocaine on systemic inflammation in the perioperative ventricular septal defect (VSD). Methods: Twenty patients, scheduled for ventricular septal defect were randomly divided into 2 groups: lidocaine and control groups. Before rebeat lidocaine 1 mg/kg was given.The venous blood samples were obtained from the central venous at the following points: after induction of anesthesia and before cardiopulmonary bypass(CPB,T1),1 h after CPB(T2),2 h after CPB(T3), and 4 h after CPB(T4). IL-6 and IL-8 were determined by radio-immunoassay. Results: Compared with those at T1, the levels of white blood cells,polymorphonuclear neutrophils,IL-6 and IL-8 increased significantly from T2 to T4 in both groups. IL-6 and IL-8 levels reached the peak at T2. Compared with those in control groups, IL-6 level decreased obviously in lidocaine group from T2 to T4, but IL-8 level remained unchanged significantly. Conclusion: Under CPB and VSD repair the systemic inflammation is obvious, reaches the peak 30 min after CPB and persists to 4 h after CPB. Perioperative administration of lidocaine is effective against the inflammation.

Objective To investigate the protective effects of poly-ADP-ribose polymerase inhibitor 3-aminobenzamide (3-AB) on lung injury in rats with severe acute pancreatitis (SAP) and to explore the mechanisms.Methods Thirty-six Wistar rats were randomly (random number) divided into three groups (n =12 for each group),namely sham operation (SO) group,SAP group and 3-AB-treated group.The model of SAP-associated lung injury was established by retrograde injection of 5％ sodium taurocholate (STC) into the biliopancreatic duct.In the treated group,3-AB in dose of 10 mg/kg was administered twice by intravenous injection 30 min before and 30 min after STC infusion.The survival rats were sacrificed 12hours after SAP modeling,and the serum amylase,lung wet/dry ratio and myeloperoxidase (MPO) activity were determined,and pathological scores of pancreas and lung tissue were evaluated under light microscope.Expressions of interleukin (IL) -1 β and IL-6 mRNA,tumor necrosis factor α (TNF-α) and inter-cellular adhesion molecule-1 (ICAM-1) protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot,respectively. Results The serum amylase level,lung wet/dry ratio and MPO activity,IL-1β and IL-6 mRNA expressions,TNF-α and ICAM-1 protein levels were dramatically increased in SAP group ( P ＜ 0.05 ).Treatment with 3-AB significantly reduced these biomarkers in 3-AB group than in SAP group (P ＜ 0.05 ).Conclusions Poly-ADP-ribose polymerase inhibitor 3-AB exerts the protective and therapeutic effects on lung injury associated with severe acute pancreatitis through inhibiting intrapulmonary MPO activity and down-regulating the expressions of IL-1 β and IL-6 mRNA as well as the levels of TNF-α,and ICAM-1.

ObjectiveTo study serum proteomic fingerprints of colorectal cancer during onset and progression and to screen tumor markers related to prognosis.Methods Serum from colorectal cancer patients, non-cancer patients, and healthy control were profiled using WCX ProteinChip or magnetic beads and analyzed by mass spectrometry. Results Seven protein peaks were found related to colorectal cancer. Several peaks were closely related to lymph node metastasis, distal organ metastasis and decreased after surgery. The diagnostic model composed of 3398.3、5477.1、8453.9 u can detect CEA negative colorectal cancer in 100%. Conclusion Protein fingerprinting technology (PFT) in conjunction with bioinformatics can significantly identify novel biomarkers in the serum of colorectal cancer patients with potential values for prognostic evaluation, detection of CEA negative colorectal cancer and changing its progression.

Objective To explore the effects of rosiglitazone on pancreatic β cells in a rat model of severe acute pancreatitis(SAP).Methods 36 male Wistar rats were randomly divided into sham operation group(SO group),SAP group,and rosiglitazone pretreatment group(ROSI group).SAP model was induced by retrograde infusion of 5％ sodium taurocholate into the biliopancreatic duct.SO and SAP group were injected 10％ dimethyl sulfoxide partes aequales(DMSO) (0.2 ml/100 g)by femoral vein 30 mins piror to the surgery.ROSI group were injected rosiglitazone (6 mg/kg)instead of 10％ DMSO.Rats were killed 12 h after surgery and the level of serum amylase was detected.Pancreas tissues were taken for pathological examination under light microscope and insulin in situ staining was done.The ultrastructure of β cells was observed with transmission electron microscope.Results Amylase level and pathologic score were increased significantly in SAP group than in SO group (P ＜ 0.05).Compared with SAP group,amylase level and pathologic score reduced significantly for rats in ROSI group (P ＜ 0.05).Compared with SAP group,the morphological structure of β cells in ROSI group was more completed and insulin in situ staining was darker observed by electronic microscopy.Conclusion ROSI exerts protective effect on morphology and endocrine function of pancreatic β cells in rats with severe acute pancreatitis.

Objective:To determine the level of epilepsy knowledge of caregivers for children with epilepsy and analyze its influencing factors, and investigate caregivers' educational needs and their acceptance for remote education, in order to provide reference for clinical telenursing education.Methods:From March to September 2022, 221 caregivers of epileptic children in the outpatient department and ward of neurology department of Xuzhou Children's Hospital were recruited by convenient sampling method for cross-sectional investigation. The status of caregivers' knowledge and educational needs were investigated by the general information questionnaire, epilepsy knowledge questionnaire, epilepsy knowledge needs questionnaire and telenursing acceptance questionnaire, and the influencing factors of knowledge level were analyzed by multiple linear regression.Results:The average score of epilepsy knowledge of caregivers was (15.68 ± 6.43) points. The course of disease, taking medicine on time, education background and monthly income of caregivers were the influencing factors of caregivers' knowledge level, and the difference was statistically significant ( P <0.05). 94.12% (208/221)- 96.38% (213/221) of the caregivers had high educational needs, and they had the highest demand for safety guidance during seizures. Caregivers' acceptance of remote education was moderate, ranging from 34.39% (76/221) to 71.95% (159/221). Conclusions:Caregivers' epilepsy knowledge needs to be improved. Medical institutions should formulate education plans according to the different characteristics of caregivers. Caregivers have a high demand for nursing knowledge, and medical staff should increase health education. Before giving health education based on remote nursing platform, we should fully understand the attitude of caregivers to the platform, so that they can master disease knowledge, strengthen their disease management ability, and improve the quality of life of children.

OBJECTIVETo explore the trend change of the morbidity and mortality of colorectal cancer in China in order to provide reference to the prevention and control of colorectal cancer.

METHODSAccording to the 1-3 volumes of "Pathogenesis and death of malignancies in pilot program city and county of China", "Pathogenesis and death of cancer in China"(2003-2007) and "Registration annual report of tumor in China" published in 2011 and 2012, data of pathogenesis and death of colorectal cancer from 10 tumor registration spots, including Beijing urban, Shanghai urban, Wuhan urban, Harbin urban (defined as city urban), and Hebei Ci County, Jiangsu Qidong District, Zhejiang Jiashan District, Guangxi Fusui County, Fujian Changle District, Henan Lin County (defined as rural district), between 1988 and 2009 were collected. The morbidity and mortality were elucidated with world population standardized rate. Ratio of pathogenesis to death was calculated with crude rate of morbidity and mortality. Data of 22 years were enrolled into the linear regression analysis to calculate the annual change rate of morbidity and mortality statistically.

RESULTS(1) Colon cancer: morbidity presented increasing trend; male morbidity in city urban increased faster; mortality presented increasing trend as well; no significant difference of increasing velocity was observed between city urban and rural district; morbidity and mortality in city urban were higher compared to rural district; morbidity and mortality of males were higher compared to females; except stable Fujian Changle District, ratio of pathogenesis to death presented decreased trend in Shanghai urban and Hebei Ci County, and increased trend in other 7 spots (all P<0.05). (2) Rectal cancer: morbidity presented increasing trend, and its increasing velocity of city urban was faster compared to rural district; mortality presented decreased trend, especially in females, and this trend in rural district was worse compared to city urban; morbidity and mortality of males were higher compared to females, while no significant difference was observed between city urban and rural district; morbidity and mortality of males and females in Zhejiang Jiashan District were all decreased (all P<0.05); except stable Harbin city, ratio of pathogenesis to death presented increased trend in other 9 spots (all P<0.05). (3) Ratio analysis of morbidity and mortality showed that percentage of colon cancer increased gradually in all 10 spots between 1988-2009.

CONCLUSIONSIn the past 2 decades, the overall morbidity and mortality of colorectal cancer are higher in city urban and in male as compared with rural district and female. Colon cancer has higher morbidity than rectal cancer and its morbidity and mortality present increased trend, while morbidity of rectal cancer presents increased trend but its mortality presents decreased trend.