Objective:To explore the effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts.Methods:32 Wistar rats were randomly divided intocontrol group,ischemia/reperfusion group(I/R group),low dose insulin postconditioning group(L-POST group)and high dose insulin postconditioning group(H-POST group),with 8 rats in each group.Langendorff and neely perfusion models were steadily established,then the rat hearts were arrested by cardioplegia(St.Thomas No.2 solution,STH-2)after perfusion for 30 min.These arrested hearts were remained for 30 min at 4℃and then underwent reperfusion for 60 min.In L-POST group and H-POST group,the arrested hearts were reperfused by Krebs-Henseleit buffer solution(K-H)with insulin.The content changes of myocardial eNOS and PKC were detected by immunohistochemistry and Western blo(tWB),the AI(Apoptosis index,AI)of cardiomyocyte was detected by TUNEL.Results:Compared with control group,higher expression of eNOS and PKC and higher AI were detected in I/R group、L-POST group and H-POST group (P

The effects of ranitidine (Ran) on cultured myocardial cells of neonatal rats were observed. Ran 100 ?mol?L-1 took no effect on spontaneous beating rates of cultured myocardial cells, but it decreased the beating rates increased by histamine 10 ?mol?L-1 and did not inhibit the beating rates increased by isoprenaline 2?mol?L-1. Histamine 10?mol?L-1 increased the amplitude, maximal rate of rise and pro-longed action potential duration at 50% (APD50) and 90% (APD90) repolarization. The effect was abolished by Ran 100?mol?L-1. The results suggested that Ran may possess antiarrhythmic action induced by histamine's increase.

0 05).③The level of the expression of AT 1a mRNA of the NO group rats was decreased significantly after hypertension of appeared and that of AT 1b mRNA of the LO group rats increased markedly after Losartan was administered. CONCLUSION Losartan has antihypertensive effects and can reverse myocardial hypertrophy induced by renovascular hypertension. Losartan regulates the mRNAs of the AT 1 receptor subtypes differently, which suggests it possibly has selectivity for AT 1a or AT 1b .

AIM To study the relation between the antiarrthymic effect and the inhibition of slow reaction cardiomyocyte of ethacizine(EC). METHODS The beating rate and action potentials of cultured slow response cardiomyocyte from neonatal rats was recorded. RESULTS EC 0 1～5 0 ?mol?L -1 produced a concentration dependent suppressing effect on spontaneous beating rate of cultured cardiac cells with IC 50 of 0 17 ?mol?L -1 and the maximal inhibitory rate was 52%.The steady state of inhibition to beating rate was achieved after adding EC for 15～30 min. The effective inhibition of beating rate by EC lasted for over 1 h.As EC 2 5 ?mol?L -1 depressed beating rate to the steady state ,effects of CaCl 2 2 mmol?L -1 and Iso 1 ?mol?L -1 on beating rate were partially reduced,and the effect of aconitine 0 2 mg?L -1 was abolished. EC 0 3～2 5 ?mol?L -1 pruduced the concentration dependent depression on APA,V max ,OS,SP 4 and MDP,and prolongation of SCL of the slow response action potentials.APD 90 was also lengthened at the concentration of EC 0 9～2 5 ?mol?L -1 . CONCLUSIONS The effective depression of EC on abnormal automaticity and conductance of the slow response cardiomyocyte in heart ischemic zone may be an important mechanism for its effective termination on some arrhythimas.EC may depress Ca 2+ ,especially Na + currents on slow response cardiomyocyte.

Objective To discuss the applied value of multislice spiral CT(MSCT) in diagnosing and localizing the embedded supernumerary teeth. Methods A total of 43 cases with the embedded supernumerary teeth were undergone MSCT using shaded surface display(SSD), minimum intensity projection(MIP) and multiplanar reformation(MPR) to present the form and location of the embedded supernumerary teeth and to determine the surgical style.The result of image was retrospectively analysed after operation.Results The reconstruction images,could clearly show the dental surface image including crown, root, neck and root bifurcation, labial or palatal location, eruption orientation and relation with dentition in anterior maxilla. The position of 52 embedded supernumerary teeth in 43 patients were completely confirmed by surgery with accuracy of 100%. All patients were well healed with no infection and injury to nearby teeth. Conclusion The Embedded supernumerary teeth in bone can be detected by MSCT and MSCT can be used as an important examination technique before surgical and orthodontic treatment.

Objective:To clarify the essence of hospital equipment management and propose the task and development trend of information management need to be accomplished, and solve the bottleneck problem of system design, which is always focused on the process of hospital equipment information management promotion in our country.Methods: Comprehensively analyzed daily work of hospital equipment management, and designed a new dual core equipment information system architecture, which was ex purchasing + post purchasing dual core architecture.Results: Based on the new architecture, the hospital equipment information system can cover the entire equipment management workflow, and it made information transmission between jobs more efficiently, cooperation more closely, responsibilities more clearly, constraint more effectively.Conclusion:The new system architecture emphasizes the role of collective labor. It depends on all the members of the equipment department to work together for the goal of achieving dynamic and full life-cycle equipment management.

Objective To investigate the clinical value of radioactive seed 125Ⅰ implantation combined with gemcitabine(GEM)in treatment for peripheral non-small cell lung cancer(NSCLC).Methods 60 patients(male 35,female 27,mean age 73.3)with peripheral lung carcinoma were confirmed under CT-guided biopsy including 8 bronchoalveolar caicinomas and 17 squamous carcinomas,6 in the stage I,14 in the stage II,34 in the stage III and 6 in the stage IV were designated under clinical staging.All patients were devided into two groups as GEM group and GEM-125Ⅰ group.GEM group underwent chemotherapy with GEM only and GEM-125Ⅰ group was treated under CT guided radioactive seed 125Ⅰ implantation combined with gemcitabine.Results GEM group showed tumors under controlling as PR in 5,SD in 14,PD in 11 cases;the total effective rate was 16% and the total control rate was 63%.The median survival time was 7 months and one years survival rate was 26%.GEM-125Ⅰ group revealed CR in 5,PR in 11,SD in 8,PD in 6 cases and the total effective rate reached 36% with total control rate as 80%.The median survival time was 12.3 months and one year survival rate was 50%.There was a significant difference(P

Objective: To investigate the application value of diffusion kurtosis imaging (DKI) for evaluating brain parenchyma microstructures changes in hypertensive patients with normal blood pressure control. Methods: Totally 22 patients with primary hypertension with blood pressure controlled in normal range were enrolled in observation group, while 19 healthy volunteers were selected as control group. DKI was performed in all subjects, and the mean kurtosis (MK), kurtosis anisotropy (KA), axial kurtosis (AK) and radial kurtosis (RK) of each brain area were measured and compared between the two groups. Results: MK values in right frontal lobe, right inner capsule knee and right cerebellum of observation group were lower than that of control group, whereas in right parietal lobe was higher than in control group(all P<0.05). The pontine KA value in observation group was lower than that in control group (P<0.05). AK values of left parietal lobe and right pallidum of observation group were lower than that in control group, of left red nucleus, right cerebellum and pontine were higher than that in control group (all P<0.05). The values of RK in right inner capsule knee, right cerebellum and left occipital lobe in observation group were lower than that in control group, and of right putamen was higher than that in control group (all P<0.05). Conclusion: DKI can be used to monitor changes of brain parenchyma microstructures in hypertensive patients with normal blood pressure control.

AIM To study the effects of Lotusine(Lot), a pure alkaloid extracted from the green seed embryo of Nelumbo nucifera Gaertn, on the action potentials(APs) in myocardium and slow inward current(Isi) in cardiac Purkinje fibers(PF). METHODS standard microelectrode and two microelectrodes voltage clamp techniques were used. RESULTS Lot 1～100 ?mol?L -1 could concentration dependently prolonged APD 20 and APD 90 of fast AP and increase the contractile force(Fc) in guinea pig papillary muscles. In papillary muscles pretreated with reserpine, similar results were also observed but the effect of Lot was weaker than those pretreated without reserpine. In guinea pig left atria, Lot 30 ?mol?L -1 could partly antigoniste the shortening APD effect of acetylcholine. Lot 3～100 ?mol?L -1 could enhance the action potential amplitude(APA)and maximal velocity of phase 0 depolarization ( V max ) of slow AP of papillary muscles induced by high K + (24 mmol?L -1 )and isolated sinoatral node(SAN) pacemaker cells of rabbits with dose dependent manner,But not obvousely short the sinus cycle length of SAN. Moreover, Lot increased the Isi of in canin cardiac PF with with time dependent and dose dependent manner. CONCLUSION The results suggest that Lot has effect of prolongation of APD and increase of Ca 2+ influx and these are very important in contribution to its positive inotropic effect, which may be related to inhibition of phosphodiesterase Ⅲ.

Objective To study that Geranylgeranylacetone(GA) induce the expression of heat shock protein 70 expression in hippocampus of Alzheimer's model rats and its effect on learning and memory ability in the model rats induced by Aβ1-42 injection into hippocampus.Method 72 health SD rats were randomly divided into GGA group,model group was injected with Aβ1-42 and control group was injected with normal saline into hippocampus.Y maze test was used to detect the learning and memory ability of the rats at the 7th,14th and 21st day after injection into hippocampus separately.HSP70 expression in hippocampus tissues were detected by RT-PCR and western-blot as soon as Y maze test has been finished.Result The learning and memory ability of the rats in model group decreased significantly at the 14th and 21st day after injection than those in control group(P<0.05),but not too much changed in GGA group(P<0.05).HSP70 expres- sions in hippocampus tissues in model group decreased gradually after injection Aβ1-42,but increased in GGA group at the 7th,14th and 21st day after injection.Conclusion GGA can induce the expression of HSP70 in hippocarnpus of Alzheimer's model rats and meliorate the neuron impairment as well as the learning and memory ability of the rats.