Oxidative stress induced injury mediated by active oxygen radicals(AOR) is one of important pathophysiologic characteristics in critical illness such as severe trauma,shock,sepsis and pancreatitis.Large amounts of AOR can be generated and the function of antioxidant system will be severely compromised with the result of an imbalance between oxidative and anti oxidative.Supplementation with anti oxidant via nutrition supports may be an important way to reduce oxidative stress induced injury in critical care.But there are some problems such as the timing,methods,dosage,safety and effectiveness of this regime.

Objective To study the treatment of neonate-infants after open-heart surgery during perioperafive period.Methods The clinical data of 1619 neonate-infants during perioperative period were analyzed.Results 68 cases died,mortality 4.2%.Postoperative complication 190 cases(11.7%).Conclusion The management of components and quantities of the infusion,admistration of cardiovascular drugs,the management of respiratory tracts and the protection of heart function,ale main points of perioperafive therapy of neonate-infants after open-heart surgery.

Objective To analyse the DSA findings of hepatic alveolar echinococcus (HAE) and evaluate the feasibility of the interventional therapy.Methods Eight patients with HAE were all examined by DSA , CT and lab test . Three of them were performed the transcatheter arterial embolization by iodlized oil. All of them were confirmed by surgical operation and followed-up for three months. Results The DSA findings of HAE included hepatic arterial curling and enlargement with hypertrophied circled encircling as typical “handed ball”. Eight cases presented the ring stain during the capillary stage. After embolization, the retention of iodized oil were revealed by DSA in 3 cases. CT three months later showed fine iodized oil retention and the enlarged necrosis. Ischemic necrosis around the lesion and the cut margins were clearly seen in operation biopsy.Conclusions HAE possesses special DSA findings and interventional therapy in a new therapeutic method of choice.

Objective To evaluate the diagnostic value of digital subtraction angiography (DSA) of intracranial meningioma.Methods DSA was performed in 115 cases with meningioma. The branches of the external carotid artery were embolized in 76 cases. Results The blood supply of the meningioma located at the relatively superficial cerebrum are mainly arising from the middle meningeal artery of the internal maxillary artery and those located at the skull base are mainly from the ophthalmic artery and the cerebral segment of the internal carotid artery. The possible dangerous anastomoses between the extermal and internal carotid arteries and the vertebrobasilar arteries may exist.Conclusions To have a good knowledge of the charactereristics of digital subtraction angiography of meningioma is crucial for endovascular embolization and operation of meningioma.

Objective To investigate the clinical characteristics and outcome of the subgroups of class V lupus nephritis (LN) . Methods 152 patients with class V LN proven by renal biopsy were classified into 2 subgroups according to the 1995 WHO modified classification of LN. Class Va (61 patients) referred to pure membranous glomerulonephritis. Class Vb (91 patients) referred to diffuse membranous glomerulonephritis with superimposed mesangial widening and/or mild to moderate hypercellularity, withdeposition of immune complex in the mesangial region. 488 patients with class Ⅳ LN in the same period werechosen as control. The clinical manifestations, serology examination, transformation of histopathological pattern and prognosis were compared between patients with class Va and class Vb. Results The incidence of hypertension, anemia in patients with class Vb were higher than that in class Va significantly (38. 5% vs 21. 3% , 72. 5% vs 52. 5% respectively, P

Objective To observe the morphological changes of hairs in two patients with loose anagen hair syndrome.Methods Light microscopy,scanning electron microscopy and transmission electron microscopy were used to observe the morphology of hair shaft and follicles from two patients,including a 3-year-old girl child and her mother,with loose anagen hair syndrome.Results Light microscopy revealed that hair bulb was deformed,hair shaft was distorted,damaged,and even disrupted,and hairs tapered in diameter at their distal end.Scanning electron microscopy showed deformed or distorted hair shaft and wave-like edge of hair cuticles.Transmission electron microscopy revealed that pathological changes were mainly localized in the inner root sheath with vacuolization in both inner and outer root sheath cells.Intercellular adhesion was weak with a decrease or disappearance of desmosomes.Conclusion The pathological changes of hairs are mainly localized in the inner root sheath in patients with loose anagen hair syndrome.

Objective To investigate the effect on NO、IL-10、CRP and bacterial translocation rate by glutamine enriched parenteral nutrition solution in purulent appendicitis in children.Methods72 cases of acute suppurative appendicitis complicated with peritonitis were selected and randomLy divided into control and treatment groups.Patients (36 cases) in the control group were given antibiotics and parenteral nutrition support for 7 days, 36 cases in the experiment group were given glutamine enriched parenteral nutrition solution for 7 days.NO、IL-10、 CRP and bacterial translocation rate were compared after the treatment.ResultsCompared with before treatment, two groups of patients with content of FT3, FT4 was higher(P<0.05), CRP, NO and IL-10、 content in serum was lower (P<0.05), 16Sr and BG positive rate was lower (P<0.05).Compared with the control group, the serum FT3, FT4 was higher(P<0.05), CRP, IL-8 and IL-6 content in serum was lower (P<0.05), 16Sr and BG positive rate was lower (P<0.05).ConclusionGlutamine enriched parenteral nutrition solution can significantly improve thyroid hormone levels,reduce serum CRP, bacterial translocation rate, and adverse reactions were mild.

Objective:To clarify the essence of hospital equipment management and propose the task and development trend of information management need to be accomplished, and solve the bottleneck problem of system design, which is always focused on the process of hospital equipment information management promotion in our country.Methods: Comprehensively analyzed daily work of hospital equipment management, and designed a new dual core equipment information system architecture, which was ex purchasing + post purchasing dual core architecture.Results: Based on the new architecture, the hospital equipment information system can cover the entire equipment management workflow, and it made information transmission between jobs more efficiently, cooperation more closely, responsibilities more clearly, constraint more effectively.Conclusion:The new system architecture emphasizes the role of collective labor. It depends on all the members of the equipment department to work together for the goal of achieving dynamic and full life-cycle equipment management.

Objectives: To investigate the effect of butyrate, the by-products of diet fiber fermented in colon, on the apoptosis of human colon cancer cell and its mechanism for colon cancer prevention. Methods: The subculture of human colon cancer cell line SW1116 was cultured in medium without or with different concentration of butyrate(2、3、4、7 and 10 mmol/L). The cell proliferation rate and apoptosis were measured by MTT and flow cytometer or electro-microscopy respectively after 6、24、48 and 72 cultivation. Results: By the increasing of butyrate concentration and cell culture time, the proliferation of SW1116 cell was inhibited gradually with a highest rate of 59.19% during the study period. The percent of G 1 cell was increased gradually whereas the percent of S cell was decreased. The apoptosis rate of SW1116 was increased gradually with a highest rate of 30.62% at the same time. The ultrastructure of SW1116 cell was changed after butyrate was added to culture medium. Conclusions: Butyrate could not only inhibit the proliferation of colon cancer cell line but also induce it apoptosis.

Objective: To investigate the mechanism of butyrate,which is the by-products of diet fiber fermented in colon and induce the apoptosis of human colon cancer cell. Methods: The subculture of human colon cancer cell line SW1116 was cultured in medium with different concentration of butyrate(0、2 、3 、4、7 and 10 mmol/L).The mRNA and proteins of bcl-2 or bax in cell lines were detected respectively by RT-PCR and Western Blotting while the concentration of p53 protein and the activity of caspase-3 was detected by flow cytometer and fluorescent quantitative assay respectively after 6、24、48 and 72h cultivation.Results: There was no expression of bcl-2 mRNA and its protein in SW1116 cell during the study periods but the expression of bax mRNA and its protein,the p53 protein concentration and the caspase-3 activity increased gradually when the butyrate concentration was increased and the cultivation time was prolonged. Conclusion: By up-regulating the expression of bax mRNA and its protein,butyrate induce the apoptosis of colon cancer cell on a p53 dependent way.