Objective To establish a method for the determination of baicalin in Huangqin Eye Drops by HPLC. Methods A HPLC was performed on Shim- pack VP- ODS column(4.6 mm? 250 mm), a mixture of methanol- 0.2 % phosphoric acid (44 ∶ 55)was used as the mobile phase and the detection wavelength was 276nm. The flow rate was 1.0 mL/min with injection volume of 20 ? L and the column temperature was 35 ℃ .Results The linearity of baicalin was good in the range of 0.08～ 0.80 ? g (r=0.9997), and the average recovery was 100.10 % , with RSD 0.81 % . Conclusion This method was simple, sensitive and accurate , and can be used for quality control of Huangqin Eye Drops.

OBJECTIVETo explore the impacts on EC50 in the remifentanil inhibition of tracheal intubation response by the transcutaneous electrical acupoint stimulation (TEAS) at Hegu (LI 4) and Neiguan (PC 6).

METHODSForty patients with selective surgery undergoing endotracheal intubation with intravenous general anesthesia were divided into I to II degree by the American Society of Anesthesiologists (ASA) and were randomized into an observation group and a control group, 20 cases in each one. Before general anesthesia induction, in the observation group, the transcutaneous electric stimulation was applied to bilateral Hegu (LI 4) and Neiguan (PC 6) for 30 min, with dense-disperse wave, 2 Hz/100 Hz in frequency; in the control group, the sham-stimulation was applied to the acupoints, with the lamp on, but without electric current output. Afterwards, the general anesthesia induction started. When the target concentration of propofol and remifentanil was stabilized at the preset value, the endotracheal intubation was conducted. Dixon sequential method was applied for the determination of ECs in remifentanil inhibition of tracheal intubation response.

RESULTSThe level of EC50 in remifentanil inhibition of tracheal intubation response was 3. 46 ng/mL, 95% confidence interval was 2. 80 ng/ml to 4. 27ng/mL in the observation group; those were 4. 18 ng/mL and 3. 30 ng/mL to 5. 29 ng/mL in the control group separately. The differences were significant in comparison of the two groups (P<0. 01).

CONCLUSIONTEAS apparently reduces EChe in the remifentanil inhibition of tracheal intubation response by around 17%as.

Acupuncture Points ; Adult ; Aged ; Anesthetics, Intravenous ; administration & dosage ; Female ; Humans ; Intubation, Intratracheal ; adverse effects ; Male ; Middle Aged ; Pain ; etiology ; Pain Management ; Piperidines ; administration & dosage ; Transcutaneous Electric Nerve Stimulation

In this study, antibody-dependent murine neutrophil-mediated cytotoxicity (ADCC)against schistosomula of Schistosoma japonicum (Chinese strain) were demonstrated in vitro by use of immune effector mechanism analysis system. The results showed that neutrophils from the normal mice could mediate the antibody-dependent ADCC reaction against the schistosomula of Schistosoma japonicum, This ADCC reaction was not dependent on the complements, the complements could not enhance the ADCC reaction mediated by the neutrophils.

Objective To investigate the effect of aprotinin on the inflammation response to reinfusion of shed blood during operation after being aspirated, filtered and washed using cell saver.Methods Twenty-four ASA I - II patients undergoing elective spinal surgery under general anesthesia were randomly divided into 2 groups: aprotinin group ( n = 12) and control group ( n = 12) .The patients were premedicated with intramuscular pentobarbital 0.1 g and atropine 0.5 mg. Anesthesia was induced with propofol 2 mg ? kg -1 , fentanyl 2-4 ?g ? kg-1 and vecuronium 0.1-0.3 mg ? kg-1 and maintained with isoflurane, propofol infusion and intermittent i. v. boluses of fentanyl and vecuronium. The patients were mechanically ventilated after tracheal intubation. In aprotinin group aprotinin 10 ? 105 IU was given before skin incision and another dose of aprotinin 10 ? 105 IU was continuously infused during operation. The blood shed during operation was collected, anticoagulated with heparin, filtered, washed and reinfused using AutoLog cell saver. Blood samples were taken from CVP line before skin incision (T1 , baseline), 30 min after reinfusion of salvaged bloods (T2) and at the end of operation (T3 ) for WBC and neutrophil granulocyte counts and determination of expression of CD11b and CD18 on the surface of neutrophil granulocytes using flow cytometry. Results The two groups were similar with respect to the general condition of the patients, duration of operation and account of shed blood reinfused. (676? 353) ml was reinfused in control group. The expression of CD11b and CD18 on the surface of neutrophil granulocytes increased significantly at T2 and T3 as compared to the baseline at T1 in control group ( P

Objective: To evaluate the effects of isoflurane-nitrous oxide on hemodynamics in patients undergoing cardiac valve replacement. Method:Hemodynamics values were measured with Swan-Ganz technique. Thirty-nine patients were divided into two groups according to their cardiac function before surgery. The patients with cardiac function Ⅱ degree were belong to group Ⅰ and those with cardiac function Ⅲ-Ⅳ degree to group Ⅱ. Result:CI,LVWI, RVWI,HR and MAP decreased significantly in both groups after induction of anesthesia without change in SI. During tracheal intubation and sternotomy,CI and LVWI decreased,PTRI and SVRI increased,particularly in group Ⅱ. During aorta and vena cava intubation the changes of hemodynamics were relatively complicated with CI and SI improved in group Ⅱ and further decreased in group Ⅰ. After CPB,MPAP,PAWP,PTRI and SVRI were decreasad in different degree in both groups with significant increased in CI and SI in group Ⅱ. Conclusion:Induction and maintenance anes thesia with 1.0%-1.5% isoflurane and 40%-50% N_2O are suitable in patients with impaired cardiac function.

Objective: To observe the changes of the pulmonary ventilatory function during cardiac valve operation under cardiopulmonary bypass(CPB). Method: Thirty-four patients undergoing cardiac valve operation were selected. The pulmonary function was measured with side stream spirometer. Result: P_(peak) and P_(plat) did not have any significant changes within 60 min after CPB, but increased significantly at the end of surgery and after closure of sternum(P

Objective To study the effects of sitting position on pulmonary hemodynamics and intrapulmonary shunt (Qs/Qt) during neurosurgery. Methods Twenty eight patients scheduled for posterior fossa and posterior cervical spinal surgery, were anesthetized with sevoflurane fentanyl pancuronium or enflurane fentanyl pancuronium after intravenous induction. Pulmonary hemodynamics were monitored with Swan Ganz catheter technique and Qs/Qt was calculated from the data of arterial and mixture venous blood gas analysis, including the following parameters: CI, RAP, MPAP, PCWP, PVRI, pHa, PaCO 2, PaO 2, PaO 2/FiO 2 and Qs/Qt. The detection time points were as the follow: before anesthesia induction, before sitting position, immediately ,60 min and 120min after sitting position, and at the supine position after termination of surgery.Results After induction of anesthesia and during operation at sitting position, CI decreased significantly (P0.05).Conclusions During neurosurgical procedure under general anesthesia and sitting position, pulmonary vascular auto regulation can be preserved and pulmonary oxygenation can be kept in normal.

Oxygen free radical, an important risk factor for cerebral infarction, plays an important role in the formation of atherosclerosis, and it is one of the major factors of cerebral isehemia-reperfusion injury after cerebral infarction. Glutathione peroxidase (GPx) is a crucial antioxidant enzyme, its main role is to scavenge the excessive oxygen free radicals produced from metabolism or during the oxidative stress. The deficiency of GPx will increase the risk of cerebral infarction. This article reviews the biological characteristics of GPx and its correlation with cerebral infarction.

Objective To study the change and its correlation between plasma concentration of glutathione peroxidase and polymorphisms of the glutathione peroxidase(GPx-3) promoter gene in patients with acute cerebral infarction(ACI).Methods 94 patients with ACI and 80 normal controls were detected the concentration of plasma glutathione peroxidas.The patients with ACI were undertaken neural function deficient scale(NDS) after 1 d onset.GPx-3 gene polymorphism were detected using polymerase chain reaction and ligase detection reaction(PCR-LDR) in patients with ACI.Results Plasma glutathione peroxidase concentration in ACI group were lower than that in the normal control group in first day onset(P0.05).The plasma concentration of glutathione peroxidase and NDS in patients with mutation of GPx-3 promoter gene were obviously lower than those in the patients without mutation(P

Naive and chronically infected C57BL/6 mice were challenged percutaneously over the ear pinna with Schistosoma japonicum cercariae. After 15 hours, the number of EOS increased significantly in the skin of chronically infected mice. Inflammatory cells aggre-gated in the vicinity of schisto.somula or entrapped intact and disintegrated schistosomula, forming granulocytic micro-abscesses in both groups. Ultrastructure studies revealed that flattened EOS tightly attached to the schistosomulum surface and degranulated. Local tegument damage occurred in the area of attacbment. NEU adherence did not seem to be as intimate as EOS, and degranulation was not seen. The tegument of the attached schis-tosoniulum remained normal. The result suggested that EOS appeared to be the efficient killer cell against skin phase schistosomula of S. japonicum (Figs. 1-6).