OBJECTIVE:To establish a RP-HPLC method for the determination of hydroxy safflor yellow A in Orthopae_dics lotion 1.METHODS:The separation was performed on Phenomenex Luna C18 with mobile phase composed of methanol-acetonitrile-water(26∶ 2∶ 72).The flow rate was 1.0ml/min;the detective wavelength was 403nm and the column temperature was 30℃.RESULTS:At a sample size of 0.1 044? g～ 1.2 528? g(r=0.9 998),hydroxy safflor yellow A was noted to be of good linear relation with peak area score.The average recovery was 98.08%(RSD=0.52%).CONCLUSION:The method is simple,accurate,specific,sensitive,reproducible,and suitable for the quality control of Orthopaedics lotion 1.

Objective To explore the role ofmirror image on arterial lesion severity of acute ST segment elevation myocardial infarction (STEMI) patients with electrocardiograrn(ECG) reciprocal lead ST segment,as well as its clinical prognosis.Methods Totally 167 STEMI patients with completed data were enrolled in present study.The patients were divided into four groups according to the amplitude of corresponding lead ST segment.Group Ⅰ consisted of 33 patients with non ST depression and group Ⅱ consisted of 97 patients whose amplitude of corresponding lead ST segment were less or equal to the amplitude of the ST segment elevation in myocardial infarction area.Group Ⅲ consisted of 25 patients whose amplitude of corresponding lead ST segment was larger than the amplitude of the ST segment elevation and group Ⅳconsisted of 12 patients whose corresponding ST segment and ST segment in myocardial infarction area were all elevated.The culprit artery lesion and clinical prognosis were compared between the four groups.Results There were significant differences in terms of the extent of lesion stenosis and the number of coronary artery stenosis among the four groups (x2 =13.49,P＜0.05;x2=78.34,P＜0.01).In Ⅳ group,the prognosis indices included pump failure,low blood pressure,malignant arrhythmia,ventricular wall incoordination,left ventricular ejection fraction ≤ 50％ and inhospital mortality significantly increased(P＜0.01).Conclusion Different corresponding ST segment depression in patients with acute ST segment elevation myocardial infarction can forecast the culprit artery lesion and clinical prognosis.

This study was aimed to investigate the research on pharmacological effects, relevant ideas and methods of Guangdong native heat-clearing and toxin-removing herbs. Literatures on pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs were collected, summarized and analyzed. The results showed that most Guangdong native heat-clearing and toxin-removing herbs had obvious effects of anti-microbial, anti-bacterial endotoxin, anti-inflammatory, and antipyretic effect. It also had obvious antivenomous and analgesia effect. Based on research ideas and methods of Guangdong native heat-clearing and toxin-removing herbs, we clarified the confusion species as soon as possible, and adhered to the whole animal experiments in combination with in vitro experiments and computer molecular docking simulations for the complementation of each other. It was concluded that the study on pharmacological effects of Guangdongnative heat-clearing and toxin-removing herbs provided strong support in its clinical application. Ideas and methods provided in this article was the main way to explain pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs.

OBJECTIVE:To provide reference for better carrying out safe medication intervention for the empty-nest elderly.METHODS:Two communities with different levels of income and culture were collected from Pudong New District of Shanghai,i.e.new community A and traditional community B.By cluster sampling,69 and 110 empty-nest elderly were selected as respondents respectively.The questionnaire survey about drug storage and medication habits of the elderly was conducted.The data of the survey were analyzed statistically.RESULTS:A total of 69 and 110 questionnaires were sent out,and 44 and 63 questionnaires were recovered with effective recovery rate of 63.8％ and 57.3％,respectively.The rate of drug storage in families from traditional community B was higher than new community A (100％ vs.88.6％,P=0.010).The proportions of residents in two communities storing their drugs in special drawers were the highest (59.0％ and 57.1％).Nearly half of the surveyed households in 2 communities did not form the habit of regularly clearing stored drugs.The frequency of residents who cleared stored drugs in traditional community B was higher than those in new community A (P=0.009).Nearly more than 70％ of the residents threw their expired drugs to garbage and only 11％ to drug stores or community health service center.The proportion of residents who were used to reading drug instructions in new community A was higher than traditional community B (95.5％ vs.77.8％,P=0.024).The proportion of the residents consulted community doctors when they were puzzled by drug use in new community A was lower than traditional community B (56.8％ vs.76.2％);The proportion of the residents consulted physicians in major hospitals was higher than traditional community B (34.1％ vs.9.5 ％);there was statistical significance (P=0.034).The most popular health service for the residents in the new community A was the hotline for the drug use (65.9％),while the residents in traditional community B needed blood pressure and sugar monitoring service and set up the health records (41.3％).The proportion of the residents' following demands in new community A was higher than traditional community B:opening drug use hotline,publicizing the knowledge of diseases and medication and conducting lectures (P＜0.001,P=0.006,P=0.018).CONCLUSIONS:The proportion of drug storage in empty-nest families of two communities is in very high level,but drug storage methods,cleaning frequency are not satisfactory.There are still a small number of elderly people who do not have the habit of reading drug instructions.There are some differences between the old and new communities.At the same time,the needs of health services in new and old communities are different.

Objective:To explore any correlation between ratings using item d550 of the International Classification of Functioning, Disability and Health (ICF-d550) and the nutritional status of patients with cerebrovascular disease.Methods:Nutritional assessments of 260 patients with cerebrovascular diseases hospitalized in a geriatric care hospital were carried out using the ICF-d550 and NRS2002 nutrition risk screening. The enter method was used to analyze the relationship between clinical factors and the scales. Spearman correlation coefficients quantified the correlation between ICF-d550 and NRS2002 results and the influence of clinical factors.Results:According to the ICF-d550 ratings the incidence of nutritional disorders was 85.00% while the NRS2002 reported 68.85%. The correlation coefficient between the two sets of results was 0.49, with the correlation coefficient of the males (0.53) slightly higher than that of the females (0.43). The correlation strengthened with age. The correlation coefficients of thin, normal and overweight persons were 0.61, 0.44 and 0.50 respectively.Conclusions:The ICF-d550 and NRS2002 can both be used to evaluate the nutritional risk of elderly patients with cerebrovascular disease, with good correlation.

Objective To develop a method for rapid determination of the dissolution of cefixime capsules,to explore the consistency of the dissolution curves of the generic and reference preparations in different media,and to assess the reliability of the in vitro dissolution evaluation method according to the results of bioequivalence studies.Methods The dissolution test was performed by the paddle method at 50 r·min-1,using pH1.2 hydrochloric acid solution,pH6.8 phosphate buffer solution,pH7.5 phosphate buffer solution,and water as the dissolution media.And a high-performance liquid chromatography(HPLC)method with a core-shell column was established to determine the dissolution curves of the generic and reference preparations respectively.The bioequivalence of the generic and reference preparations was evaluated through the bioequivalence(BE)test.Results The similarity factors(f2)of the three batches of the generic and reference preparations in the four media were greater than 60.The fasting and postprandial pharmacokinetic parameters(Cmax,AUC0-t,AUC0-∞)of the generic and reference preparations in hu-mans were all in line with the bioequivalence standard.Conclusion The in vitro dissolution behavior of the generic and refer-ence preparations was consistent,and the two preparations were bioequivalent.The method is simple and quick,and it can be em-ployed to measure the dissolution of cefixime capsules,which can provide references for the consistency evaluation of cefixime capsules.

Cyanobacteria are the only prokaryotes capable of oxygenic photosynthesis, which have potential to serve as "autotrophic cell factories". However, the synthesis of biofuels and chemicals using cyanobacteria as chassis are suffered from poor stress tolerance and low yield, resulting in low economic feasibility for industrial production. Thus, it's urgent to construct new cyanobacterial chassis by means of synthetic biology. In recent years, adaptive laboratory evolution (ALE) has made great achievements in chassis engineering, including optimizing growth rate, increasing tolerance, enhancing substrate utilization and increasing product yield. ALE has also made some progress in improving the tolerance of cyanobacteria to high light intensity, heavy metal ions, high concentrations of salt and organic solvents. However, the engineering efficiency of ALE strategy in cyanobacteria is generally low, and the molecular mechanisms underpinning the tolerance to various stresses have not been fully elucidated. To this end, this review summarizes the ALE-associated technical strategies and their applications in cyanobacteria chassis engineering, following by discussing how to construct larger ALE mutation library, increase mutation frequency of strains and shorten evolution time. Moreover, exploration of the construction principles and strategies for constructing multi-stress tolerant cyanobacteria, and efficient analysis the mutant libraries of evolved strains as well as construction of strains with high yield and strong robustness are discussed, with the aim to facilitate the engineering of cyanobacteria chassis and the application of engineered cyanobacteria in the future.
Technology ; Photosynthesis/genetics* ; Cyanobacteria/genetics* ; Light ; Biofuels