ObjectiveTo study the effect of high-dose mifepristone on the expression of hypoxia-inducible factor 1,(HIF-1α) and vascular endothelial growth factor(VEGF) in the endometrial tissue and research the molecular mechanisms of mifepristone in treat ment of endometrial cancer.Methods30 patients with endometrial cancer who were treated were asked to oral mifepristone 100mg/d,the specimens from a biopsy or curettage were taken as the group before treatment,the specimens from surgery were as the group of after treatment.Another 10 cases of normal endometrium were selected as the control group.The expression of HIF-1α and VEGF were examined with immunohistochemistry.ResultsThe expression of HIF-1 α and VEGF(78.6 ± 3.5,92.4 ± 5.1 ) in endometrial cancer was significantly higher than normal endometrium( 192.3 ± 4.7,178.4 ± 3.3,P ＜ 0.01 ),large doses of mifepristone can significantly reduce the expression of HIF-1 α and VEGF in endometrial cancer issues ( 103.2 ± 4.1,118.3 ± 4.9,P ＜0.05).ConclusionOne of the molecular mechanisms of mifepristone inhibits the endometrial cancer was reduced HIF-1α and VEGF expression.

Objective To investigate the content change of aconitine in Radix Aconiti Kusnezoffii in different boiling time. Methods The chromatographic procedure was carried out with methanol-water-chloroform-diethylamide (70∶30∶3∶0.2) as mobile phase at a flow rate of 1.0 mL/min, the column temperature at 30 ℃, and UV detector at 240 nm. Results There was a good linear relationship of aconitine within 0.08-0.8 μg, r=0.999 8 (n=6), and the average recovery rate was 97.16%. The content of aconitine increased gradually in 0-2 h, then gradually decreased. And the reduction was evident particularly during 2-4 hours. Conclusion HPLC method is rapid, simple, accurate, reproducible and reliable for determining aconitine. The content of aconitine can be decreased effectively by boiling 4 hours.

OBJECTIVE:To evaluate the effects of Sennae Folium combined with mannitol for bowel cleaning on multi-slice spiral CT bowel imaging. METHODS:52 patients receiving multi-slice spiral CT imaging examination in our hospital during Jun. 2011 to Jun. 2012 were collected retrospectively,and then divided into control group(22 cases)and observation group(30 cases) according to the methods of bowel preparation. Both groups fasted for 12 h before examination. Sennae Folium 20 g soaked in boiled water 500 ml for half an hour was given to patients in control group 12 h before examination,and then was soaked in boiled water 500 ml again for drinking and other pure water 1 000 ml was also given to patients. Sennae Folium 10 g soaked in boiled wa-ter 500 ml was given to patients in observation group 1 d before examination and 2 h after breakfast;soaked in boiled water 500 ml again 2 h after lunch;10% mannitol 500 ml and pure water 1 000 ml were given to patients after supper. Both groups received multi-slice spiral CT bowel imaging until the patients defecated watery stool after medication. The effective rate of bowl cleaning, image quality and the incidence of ADR were compared between 2 groups. RESULTS:The effective rate of bowel cleaning was 100% in observation group,which was significantly higher than that of control group(81.82%),with statistical significance(P<0.05). The excellent and good rate of imaging quality was 96.67% in observation group,which was significantly better than that of control group(81.82%),with statistical significance(P<0.05). The incidence of ADR as abdominal distension,nausea,intestinal mucosa damage in observation group were significantly lower than those of control group,with statistical significance (P<0.05). CONCLUSIONS:Sennae Folium combined with mannitol is effective method to prepare enteric multi-slice spiral CT imaging exam-ination,and perform good bowl cleaning effect,high image quality and low incidence of ADR.

ObjectiveTo assess the relationship between the location,quantity and leukodystrophy of cerebral infarction and vascular cognitive impairment ( VCI ),and to compare the differences of the severity and domains of cognitive impairment among the different types of cerebral infarction,which will provide the pathophysiological evidence to the mechanism for the development of VCI. MethodThe patients suffering from cerebral infarction or leukodystrophy were recruited from either the ward or clinic in Tianjin General Hospital from April 2009 to April 2010,then assessed the cognition,and classified into different groups according to the location,size,quantity,whether or not they have leukodystrophy and the severity.The Logistic regression analysis was taken with whether or not the patients have had developed to the VCI as the dependent variable,and with the location,size,quantity,whether or not they have leukodystrophy and the severity as the independent variables.The differences of the score of NIHSS,Bathel index and the level of cognitive impairment were compared among patients with different types of lesions.Moreover,the difference of the score of MoCA for each cognitive domain were compared among patients with different types of lesions.ResultsThere was a relationship between key position infarction in the subcortex and white leukodystrophy with VCI.The position infarction in the subcortex and white leukodystrophy ( grade Ⅱ and Ⅲ ) might be the independent risk factors for the VCI( OR =1.752,2.135,3.753; 95％ CI 1.533-3.821,1.541-6.787,1.432-6.821,all P ＜0.05).There were significant differences in the domains of excusive ability,attention,calculation,fluent language and orientation.The average scores of the domain of excusive ability in the subcortical group (3.40 ± 1.90) and the mix group (3.83 ± 1.27) were significantly lower than the cortical disease group(4.28 ± 0.89,t =0.050,0.857,both P ＜ 0.05 ).The average scores of the domains of attention,calculation,fluent and orientation in the mix group were significantly lower than the subcortical group and cortical disease group.ConclusionsThere is a significant association of the key infarction in the subcortex and leukodystrophy with VCI.The patients with both the subcortex infarction and leukodystrophy are susceptible to VCI.The development of VCI is depended on the position of infarction instead of the quantity and size of infarction.The development of VCI is not only related to the white matter disease but also related to the severity of the leukodystrophy.The data highlights the need to small vessel disease.The patients with subcortical lesions mainly impair the executive ability.The patients with mix lesions may have more extensive domains and more severe cognitive impairment.

Objective To investigate the ultrasonographic manifestations of traumatic neuroma after neck dissection in papillary thyroid carcinoma (PTC).Methods A retrospective analysis was performed in 21 patients with 26 lesions who underwent neck dissection.The ultrasonographic characteristics were reviewed,including numbers,location,size,shape,margin,echogenicity,with or without direct continuity to the nerve fiber,the presence of internal hyperechogenicity and blood supply.Results Among the 26 lesions of 21 patients,25 lesions were in the lateral cervical area while the other one was in the central cervical area.The mean shortest diameter of the lesions was (3.5±1.0)mm and that of longest diameter was (9.1±2.9)mm.Totally 13 lesions were oval in shape and the other 13 were round.The clear margin was found in 13 lesions and fuzzy margin was observed in another 13 lesions.All the lesions were heterogeneously hypoechoic.The presence of internal hyperechoic areas were found in 16 lesions.The direct continuity with the nerve fiber was found in 20 lesions,while the other 6 lesions were not continued to the nerve directly.Color Doppler flow imaging showed that 20 of the 26 lesions existed a little blood flow signal and the other 6 lesions showed no flow signal.During the ultrasound-guided fine-needle aspiration biopsy (US-FNAB),21 patients complained about the intolerable serious pain and the pain relieved when the needle removed.Conclusion Traumatic neuromas are hypoecho lesions located in the operation area after neck dissection in PTC with distinctive ultrasonographic features,especially the direct continuity with the nerve fiber,as well as the clinical histories and sharp pain during US-FNAB,which can help to diagnose.

Objective A series of 5 patients with central pontine myelinolysis (CPM) confirmed either by cranial MRI,CT image (4 cases) or by autopsy pathological analysis (1 case) was reported. The pathophysiological mechanism,the early diagnosis and the role in prevention were discussed. Methods The creteria obtained from clinical features, cranial MRI and CT imagine as well as autopsy pathological changes were thoroughly analyzed. Results ① All 5 case of CPM were occurring in patients who had already had somatic disease and serious systemic functional failure, especially in those who had had seriously water and ion disturbances, such as hyponatremia. ② In 4 of the 5 patients, psychiatric manifestation tended to show the earliest clinical features. ③ The pseudobulbaral paralysis appeared in 4 patients. ④ Cranial MRI and CT served as the most reliable techniques for diagnosing CPM cases when patients are still alive. ⑤ A variety of paralysis were found in all of the 5 CPM patients. ⑥Pathological analysis demonstrated that there is a symmetric unspecific myelinolysis in areas of basal pontine, caudal middle brain and the superior medulla (1 patient). Conclusion ① The occurrence of hyponatrimia and its too fast correction may be associated with the onset of CPM. ② CPM, a self-restrained disease can be cured from dangerous complications, such as pneumonia, respiratory failure and malnutrition when suitably treated.

Objective To summarize continuous veno- venous hemofiltration (CVVH) treatment of infants with complex congenital heart disease in children with acute renal failure after treatment. Methods A retrospective analysis was carried out in six cases of infants with congenital heart disease with application of CVVH. The children were given monitoring and anticoagulant treatment. The renal function, electrolytes and urine volume were compared before and after the treatment. Results The amount of urine increased, blood circulation and electrolytes remained stable in six patients 17.5-135.0 hours after CVVH. Conclusions The CVVH therapy can be applied to treat infants with congenital heart disease complicated with acute renal failure, but CVVH treatment should focus on anticoagulants and blood volume since complicated state of illness, low age, low body weight of children patients.

Objective To investigate the relationship between hepatocyte growth factor (HGF) and vascular endothelial cells (VEC) in pregnancy induced hypertension (PIH) Methods Twenty women with PIH (6 mild, and 14 moderate or severe PIH ) and 20 women with normal pregnancy were recruited as study group and control group, respectively The two groups were matched in maternal age, parity and gestational age Flow cytometer was used for counting the number of peripheral blood circulating endothelial cells (CEC), while enzyme linked immunoadsordent assay (ELISA) was used to measure the concentration of serum HGF Results In the study group, the concentration of HGF was (1395?512) ng/L, and the number of CEC was (109 360?24 070) /L In the control group, the concentration of HGF was (1565 ?399) ng/L, and the number of CEC was (21 800?4420)/L The differences both in the concentration of HGF and the number of CEC between the two groups were statistically significant ( P

0.05). The positive rate of MSI in UC with dysplasia was significantly higher than that in UC (P0.05). The loss of hMSH2 protein expression was not found in UC with dysplasia and UCACRC. Conclusions Both the mutations of P53, K-ras genes and MSI are early events in UCACRC. There is no relationship between MSI and loss of hMSH2 protein expression in (UCACRC).

This article from the clinical trials of the drug inspection agency review practice of view,summarize the key elements of drug clinical trials review examination,the status of agency offices of the daily management and analysis of the problems,the agency's Office to review how the inspection standards as a guide to clinical trial program files,personnel and other standardized management were discussed,with a view of other drugs in clinical trials and routine inspection agency review management to bring new inspiration,clinical trials of drugs for hospitals and fast construction and development agencies to provide a reference.