Objective To evaluate and compare the advantages and disadvantages and the clinical application between transvaginal abdominal hysterectomy(TAH)and laparoscopically assisted vaginal hysterectomy(LAVH)on the benign lesions of non-prolapsed uterus.Methods 400 patients with benign lesions of non-prolapsed uterus were divided into two groups,TAH group(202 cases)and LAVH group(198 cases).The effects of clinical treatment of the two groups were compared.Results There were significant differentces between the two groups in operation time,the blood loss,passage of gas by anus,return to normal activities,and overall cost of treatment.However,after 1to 3 months follow-up,all patients were good in terms of outcome.Conclusion Different surgical patterns of hysterectomy had their distinctive advantages.The key point of a successful operation Was to avoid complications of the operation.

OBJECTIVE:To observe the therapeutic effect of IF ?-2b plus compound glycyrrhizin on chronic hepatitis C. METHODS:56cases of chronic hepatitis C were divided into trial group(30cases)and control group(26cases).Trial group received IF ?-2b +compound glycyrrhizin and control group received IF ?-2b alone.The therapeutic course was24weeks in both groups.RESULTS:There was significant difference in recoversion rate of ALT between two groups either at the end of ther?apeutic course or24weeks after treatment(P

Objective To investigate the changes of non-linear dynamics properties of EEG under different mental tasks,and the application of non-linear dynamic analysis for cognitive function research. Methods EEG was recorded in 30 healthy university students under four states: eyes closed,eyes open,mental arithmetic with eyes closed and graphic reasoning test with eyes open.Correlation dimension (D 2) and point-wise correlation dimension (PD 2) were calculated for all subjects.We also performed a surrogate data test in 5 randomized selected subjects. Results In all states tested,the results argued for the presence of non-linearity and fractal D 2 and PD 2.D 2 and PD 2 increased significantly during mental tasks as compared to the rest states (D 2 of above four states was 3.93,4.47,4.33 and 4.98 respectively).The results showed that values of D 2 and PD 2 fluctuated with time,which suggests an intermittent activation of the brain areas participated in the task.With non-linear dynamic analysis,there might be a working circuit from bilateral temporal lobes to the left frontal lobe and parental lobe under mental arithmetic task.Conclusions Through dynamic analysis based on D 2 and PD 2 topographic maps,we can see clearly the distribution of D 2 and PD 2 in the brain,the sequence and the degree of activation of the regions involved in the cognitive task under mental tasks.Dynamic and short-time non-linear analysis methods are more appropriate for the study of mental functions.Non-linear dynamic analysis might help us understand the working mechanism of brain during mental activities.

Objective To investigate the clinical efficacy of combined use of acupuncture and medicine in treating spasmodic torticollis.Method Thirty patients were randomly allocated to a treatment group of 15 cases and a control group of 15 cases. The treatment group received combined use of acupuncture and medicine, and the control group, acupuncture alone.Result Clinical observation lasted one year. There was a statistically significant pre-/post-treatment difference in the Tsui score in the two groups at 6 and 12 months after treatment (P<0.01). There was a statistically significant difference in the Tsui score between at 6 and 12 months after treatment in the two groups (P<0.01); the Tsui score decreased gradually after treatment. There was a statistically significant post-treatment difference in the Tsui score between the two groups at 12 months after treatment (P<0.05); the Tsui score decreased more in the treatment group.Conclusion The cure and marked efficacy rate of combined use of acupuncture and medicine for spasmodic torticollis is higher than that of acupuncture alone. A longer course is needed for acupuncture treatment of spasmodic torticollis and the patient should persevere with the treatment.

Objective To study the effects of repetitive transcranial magnetic stimulation (rTMS) on rats'locomotor function after hemisectional spinal cord injury (SCI). Methods A model of hemisectional SCI was established at the T10 segment in 12 rats,which were then randomly divided into an SCI-rTMS group and an SCI-control group.Another 6 rats served as normal controls.The SCI-rTMS group received threshold rTMS daily for 4 weeks,while the SCI-control group was given sham rTMS.The normal control group was left without any treatment.BassoBeatti-Bresnahan (BBB) locomotor scores were recorded weekly.Motor evoked potential (MEP) was detected at the 28th day after the operation.Neurofilament-200 (NF-200) was detected with immunofluorescent staining of the spinal cord lesions. Results The BBB scores in the SCI-rTMS group improved more than those in the SCI-control group.MEP of the fight hindlimb was detected in the rTMS group,but not in the control group.Expression of NF-200 marker increased significantly more than in the rTMS group. Conclusions rTMS can improve locomotor function of rats after spinal cord hemisectioning,which may result from increased expression of NF-200 and the regeneration of axons.

Pseudotemporal lobe epilepsy refers to an electroencephalogram (EEG) ictal pattern that is localized to the temporal region and the clinical ictal symptoms like the temporal seizure, especially mesial temporal seizure. But the epileptogenic zone is on the extratemporal regions. It is not easy to diagnose pseudotemporal lobe epilepsy. There are difficulties to detect by scalp EEG, and stereoelectroencephalography is usually required for epileptogenetic zone localization. Pseudotemporal lobe epilepsy almost is refractory for antiepileptic drugs. But the situation is illustrated by the failure of temporal lobe surgery resection alone. Therefore, the good result is often obtained after an epileptogenic zone and symptomatic zone resection.

Objective: To establish a GC method for determination of menthol, camphor and methylsalicylate in Cremor Mentholi Compositus. Methods: The determination was carried out on a PEG\|20M stainless steel column with temperature at 140℃, FID detector with temperature at 260℃. Results: The separating degree and the linearity were fine with the average recovery of menthol 100.17%, RSD =0.661%; camphor 99.89%, RSD =0.600%; methylsalicylate 100.17%, RSD =0.553%, respectively. Conclusions: The method is convenient, rapid and accurate. It can be used to control the quality of this preparation.

AIM: To evaluate the effect of luteolin on ulcerative colitis in experimental animals. METHODS: The mouse model of acute colitis was induced by instillation of dinitrochlorobenzene (DNCB) /alcohol. Luteolin (1, 10 and 50 mg?kg~(-1)) were orally administrated to mice for 3 days. Diarrheas, colon weight index, macroscopic damage in the colon were observed and the activity of MPO and the content of PGE_2 in colon were also examined. RESULTS: The diarrhea and the colon weight in- dex were reduced and the macroscopic damage in the inflammatory colon was effectively attenuated by luteolin. The elevated level of MPO and PGE2 were also significantly decreased. CONCLUSION: Luteolin can effectively inhibit the inflammatory infiltration, bleeding and proliferation in experimental ulcerative colitis.

AIM: To evaluate effects of rutecarpine on the movements of gastrointestinal tract in experimental animals. METHODS: In mice, the accelerated movement model of intestinal transit was induced by neostigmine, and metoclopramide or apoplon was applied to induce the accelerated gastric emptying movements. Acetylcholine or histamine was used to induce the contractions occurring in the isolated ileum from guinea pigs. RESULTS: Rutecarpine inhibited normal intestinal transit and demonstrated more effective suppression on the accelerated movement induced by neostigmine in mice; meto- clopramide and apoplon induced-accelerated gastric emptying movements were also significantly inhibited by rutecarpine in a dose-dependent manner. Meanwhile rutecarpine significantly inhibited the isolated ileum contractions induced by acetylcholine or histamine. CONCLUSION: Rutecarpine is an effective inhibitor to intestinal motility and this activity is probably mediated by its antagonistic effects on the cholinergic nerve or its responsible modulations.

Objective To discuss the application o immunohistochemistry autostainer in immunohistochemistry.Methods More than 30 kinds of first antibodies of the Pathology Department,such as AAT,CerBb-2,CK,HBcAg,ER,PR,Ki-67,and unified second antibodies were selected.Immunohistochemistry autostainer and manual operation were applied to the staining of the antibodies,and then the results by the above methods were compared.Results The antibodies stained by immunohistochemistry autostainer,gifted with clear background,no edge effect,uniform staining and accurate positive results,were all better than those by manual operation from all aspects.Conclusion Immunohistochemistry autostainer is highly automatic,time-saving,manpower-saving,repeatable and highly standardized.