College of American Pathologists (CAP) and International Standard Organization (ISO)15189 accreditation provides requirements to the verification and validation tests for clinical molecular diagnostic assays.The article will introduce the requirements in these aspects including the differences between the two accreditation systems,the differences between the verification and validation tests,the essential factors and standardized framework of the verification and validation tests for clinical molecular diagnostic assays.

Objective To study the expression of the imprinted gene H19 and IGF-Ⅱ in newborn placenta,and to discuss its influence on the birth body mass of the neonate. Methods The fresh placental tissues from full-term newborn (without trimester of pregnancy complica-tion and placenta and funic abnormality) with normal,high and low birth body mass (12,10 and 8 samples respectively)were collected. The expression of imprinted gene H19 and IGF-Ⅱ mRNA in the placenta were estimated by reakime fluorescence quantitative PCR Results The ex-pression of H19 mRNA in the placenta was negative correlation to the birth body mass (r =-0.403,P = 0.027).The expression of of IGF-H mRNA was positive correlated to the birth body mass (r = 0.444,P = 0.014). The H19 mRNA expression level in the high birth weight neonates (0.21 0.31) was significantly lower than that in the low birth body mass neonates (1.51 2.04)(P= 0.013). But the expression level of IGF-Ⅱ mRNA in the high birth body mass neonates (2.67±3.41) was significantly higher than that in the low birth body mass neonates (0.39±0.33)(P =0.013). Conclusion The expression of H19 and IGF-Ⅱ mRNA was significantly different in the placenta of normal,high and low birth body mass newboms. These two genes may be related to the birth body mass,and there may be some realation-ship between these two genes.

Ginsenoside is the main component of antitumor effects of traditional Chinese medicine (TCM) Ginseng. Clinical pharmacological studies have shown that Ginsenoside Rb1 can inhibit P-gp which can lead to efflux of drugs, enhance the sensitivity of cells to drugs, reduce the multidrug resistance of the tumor cell, and maintain the immune function of the body to tumor cells. Ginsenoside Rg3 can impact tumor cell protein expression, play a role in cell division, induce tumor cell apoptosis and inhibit tumor growth of blood vessels. Ginsenoside Rh2 may play an antitumor effect by inhibiting tumor cell proliferation and metastasis, inducing tumor cell apoptosis. This article ex-plained the antitumor effects and mechanism of Ginsenoside Rb1, Rg3, Rh2 in order to provide theoretical evidences for the clinical development and application.

Colorectal polyps are common digestive system diseases in children. The important role of endoscopy in the di-agnosis and treatment of colorectal polyps in children has been recognized. With the continuous development of endoscopic techniques, the diagnosis and treatment of colorectal polyp has become safer, more convenient and more effective. In this re-view, the progress in endoscopy for diagnosis and treatment of colorectal polyps in children has been summarized.

Objective To evaluate the influence and safety of losartan on high sensitive C-reactive protein(hs-CRP) and serum uric acid(UA) of essential high blood pressure(HBP) patients with hyperuricemia (HUA).Methods Eighty HBP patients complicated with HUA were enrolled and divided into the losartan group with losartan 50 mg/d(n =40) and the nifedipine group with nifedipine gastrointestinal therapeutic system (GITS) 30 mg/d (n =40) for 6 months continuously.The serum levels of hs-CRP,UA,hepatic and renal functions,creatine kinase(CK) and blood pressure were measured.Results Compared with before therapy,the systolic blood pressure(SBP),diastolic blood pressure (DBP) were lower in two groups after 6-month treatment and the differences were statistically significant(losartan group:SBP:(158.5 ± 13.2) mmHg vs.(136.7 ± 9.4) mmHg,t =3.50,P ＜ 0.01 ; DBP:(95.6 ± 8.4) mmHg vs.(83.3 ± 6.4) mmHg,t =3.49,P ＜ 0.01 ; nifedipine group:SBP:(157.7 ± 13.9) mmHg vs.(134.6 ± 8.2) mmHg,t =3.53,P ＜ 0.01 ; DBP:(96.1 ± 8.9) mmHg vs.(81.2±6.8) mmHg,t =3.56,P ＜0.01).The differences in terms of systolic and diastolic blood pressure were not significant at post-treatment between losartan and nifedipine group.The serum levels of hs-CRP and UA were significantly lower after 6-month treatment than before treatment in losartan group ((5.68 ± 1.53) mg/L vs.(3.52 ± 0.57) mg/L,t =3.82,P ＜ 0.01 ; (502 ± 45) μmol/L vs.(450 ± 38) μmol /L,t =3.48,P＜ 0.01),but there was no change in nifedipine group(hs-CRP:(5.61 ± 1.64) mg/L vs.(5.33 ± 1.48) mg/L,t =1.34,P ＞ 0.05 ; UA:(499 ± 43) μmol/L vs.(489 ± 42) μmol/L,t =0.68,P ＞ 0.05).There was no significant change regarding of liver and kidney functions and serum CK in losartan group before and after treatment.No adverse reaction occurred in the losartan group.Conclusion Losartan treatment can decrease serum hs-CRP and UA of HBP patients complicated with HUA,and there is no serious adverse reactions and good security during treatment.

Objective: To observe the effects of type 2 diabetes mellitus patients with hypertension on lipid metabolism and insulin sensitivity. Methods: A case-matched study of fasting plasma glucose (FBG), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HIDL), low density lipoprotein cholesterol (LDL), insulin (FIN), C-peptide (FCP) and insulin sensitivity index (ISI) were detected in type 2 diabetes mellitus patients with and without hypertensiorn. Results: Twenty patients with type 2 diabetes mellitus complicated hypertension had higher TG、 FINS and FCP, lower HDL and ISI levels than 20 patients with type 2 diabetes mellitus without hypertension. Conclusion: Type 2 diabetes mellitus with hypertension had more remarkable lipid metabolic disorder and insulin resistance than type 2 diabetes mellitus without hypertension.

Objective To investigate the relationship between self-efficacy(SE)and subjective wellbeing(SWB)of the elderly in community.Methods 212 elderly people underwent a questionnaire survey by the Memorial University of New Foundland Scale of Happiness and the General Self-efflcacy Scale.Results The level of self-efficacy of the elderly in community was(2.39±0.63),of which impact factor was the level of education and average monthly income per person.The level of SWB was(37.31±7.24),of which impact factor was the level of education and occupation.The level of SE and SWB of the elderly sas mdderately positively correhted,r=0.36,P＜0.01. Conclusions In the practice of community care services,community nurses should take SE theory as a framework and to improve SWB by a vailety of nursing measures.