Objective To study the effects of repetitive transcranial magnetic stimulation (rTMS) on rats'locomotor function after hemisectional spinal cord injury (SCI). Methods A model of hemisectional SCI was established at the T10 segment in 12 rats,which were then randomly divided into an SCI-rTMS group and an SCI-control group.Another 6 rats served as normal controls.The SCI-rTMS group received threshold rTMS daily for 4 weeks,while the SCI-control group was given sham rTMS.The normal control group was left without any treatment.BassoBeatti-Bresnahan (BBB) locomotor scores were recorded weekly.Motor evoked potential (MEP) was detected at the 28th day after the operation.Neurofilament-200 (NF-200) was detected with immunofluorescent staining of the spinal cord lesions. Results The BBB scores in the SCI-rTMS group improved more than those in the SCI-control group.MEP of the fight hindlimb was detected in the rTMS group,but not in the control group.Expression of NF-200 marker increased significantly more than in the rTMS group. Conclusions rTMS can improve locomotor function of rats after spinal cord hemisectioning,which may result from increased expression of NF-200 and the regeneration of axons.

To explore indications regularity of analogous decoctions of Erchen Decoction.323 analogous decoctions of Erchen Decoction were found in Concise Thesaurus of TCM Formula.Diseases and symptoms of TCM were analyzed.22 diseases and 118 symptoms of TCM were found.The main indication of analogous decoctions of Erchen Decoction included spleen-stomach and lung system,other symptoms were broadly involved.Erchen Decoction was the most appropriate for phlegm.

Objective To investigate the change of plasma cholinesterases (CHEs) in the people with diabetes or fatty liver or overweight , and explore the role of CHE in these diseases .Methods The plasma CHEs in 2834 subjects were detected , and these subjects were divided into five groups , including diabetes , fatty liver , overweight , diabetes with fatty liver , and the normal groups . Results The plasma CHE activities in diabetes group , fatty liver group , overweight group , and diabetes with fatty liver group were all higher than the normal group [(8943 ±1896)U/L, (9716 ±1673)U/L, (8798 ±1710)U/L, (9385 ±1687)U/L vs (8028 ±1621) U/L], and the CHE level in the fatty liver group was highest among five groups .However, the CHE level in diabetes group or fatty liv-er group was not significantly different from that in the diabetes with fatty liver group .The CHE level of the people with components of metabolic syndrome (MS) was significantly higher than that without MS component [(8786 ±1514)U/L, (9141 ±1771)U/L, (9705 ±1628)U/L, (9138 ±1768)U/L, (9530 ±1607)U/L vs (7821 ±1324)U/L]),but the CHE level was not increased gradually with the increased MS component.The plasma CHE had a negative correlation with age ( P =0.00),but it had a positive correlation with triglyceride (TG), total cholesterol (TC), and body mass index (BMI)( P =0.00).Conclusions The plasma CHE activity was el-evated in diabetes group , fatty liver group , and overweight group , which might be a risk factor in these diseases .Controlling the plas-ma CHE might help to treat the metabolism diseases .

Objective To investigate the syndromes, syndrome elements and target sites of diabetic cerebral infarction. Method Firstly, literatures were searched from January 2003 to October 2013 in China Academic Journals Database (CNKI) retrieval system, then a database of 22 eligible articles was established. Syndromes of diabetic cerebral infarction were extracted from the database, then syndromes, syndrome elements and target sites were analyzed. Results Totally 2550 cases of diabetic cerebral infarction were concerned with 15 syndromes, including 659 cases of stagnation of phlegm and blood (25.84%), 445 cases of stagnation of phlegm-heat (17.45%), 366 cases of wind-phlegm syndrome (14.35%) and 341 cases of qi deficiency and blood stasis (13.37%). There were 10 syndrome elements in the 2550 cases, of which 1588 cases with phlegm (62.27%), 1026 cases with blood stasis (40.24%), 649 cases with qi deficiency (25.45%) and 539 cases with yin deficiency (21.14%). And seven target sites were encompassed, the highlights of which included 2502 cases of liver (98.12%), 2194 cases of spleen (86.04%), 2096 cases of kidney (82.20%) and 1026 cases of brain (40.24%). Conclusion The important syndrome elements of diabetic cerebral infarction were phlegm, blood stasis, qi deficiency and yin deficiency. The four major syndromes comprising stagnation of phlegm and blood, stagnation of phlegm-heat, wind-phlegm syndrome and qi deficiency and blood stasis were concluded, mainly affected the liver, spleen, kidney and brain. And there were two fundamental pathogenesis in diabetic cerebral infarction:qi and yin deficiency, phlegm and blood stasis.

Objectives To investigate the regularity of fluctuation and clinical senses of serum c reactive protein (CRP) level in patients with acute cerebral infarction (ACI) Methods 100 subjects (56 males and 44 females) were divided into five groups including 24 large artery arteriosclerosis (LAA), 27 small artery occlusion (SAO), 2 cardiac embolism(CE), 34 other Certain(OC) and 13 undetermined etiology according to Trial of ORG 10172 in Acute Stroke Treatment (TOAST) Through enzyme linked immunoabsorbent assay (ELISA), we detected and analyzed CRP levels of 100 inpatients Results The risk factors of stroke included coronary heart disease , hypertension, diabetes and intoxication of CO and so on Among the above mentioned, the level of CRP and rate of positive CRP of patients who poisoned by CO was the highest That of coronary heart disease group was lower However, there was no notable difference between hypertension and diabetes groups Conclusions CRP may play a certain role in the occurrence and development of ACI It represents different levels in different types of stroke

The paper is to report the establishment of a population pharmacokinetic model for flurbiprofen (FP), an active metabolite of flurbiprofen axetil (FA). 246 FP serum concentration and clinical data were perspectively collected from 23 general anaesthesia patients receiving FA intravenously before operation in Dentofacial Surgery and Otorhinolaryngology Department of the First Affiliated Hospital of Fujian Medical University. Population pharmacokinetic data analysis was performed using NONMEM software. The measure of Bootstrap was applied for internal validation, while Visual Predictive check was adopted for external validation. The data of FP correspond with two-compartment model. The body weight (WT) had conspicuous effect on clearance and volume of central compartment, while sex, age and daily dose of administration had no marked effect on pharmacokinetic parameter of FP. The basic model was described as follows: CL (L x h(-1)) = 1.28x EXP(ETA(1)), V1 (L) = 5.03x EXP(ETA(2)), Q (L x h(-1)) = 8.5 x EXP(ETA(3)), V2 (L) = 4.39 x EXP(ETA(4)). The final model was described as follows: CL (L x h(-1)) = 1.32 x (WT/60) x EXP(ETA(1)), V1 (L) = 5.23 x (WT/60) x EXP(ETA(2)), Q (L x h(-1)) = 8.45 x EXP(ETA(3)), V2 (L) = 4.37 x EXP(ETA(4)). The population typical value of CL, V1, Q and V2 were: 1.32 L x h(-1), 5.23 L, 8.45 L x h(-1) and 4.37 L, respectively. Bootstrap and visual predictive check show that the final model of FP is stable, effective and predictable. A novel population pharmacokinetic model is developed to estimate the individual pharmacokinetic parameter for patients intravenous injecting FA in terms of patients' characteristics and dosing history, and to design a prior dosage regimen.

It is generally believed that liposomes modified with polyethylene glycol (PEG) have no or lower immunogenicity. However, based on many recent literatures, when the PEGylated liposomes were repeatedly applied to the same animal, the immune responses occurred. The first injection of PEGylated liposomes resulted in a reduction in the circulation time and an increase in hepatic and splenic accumulation of the second dose of PEGylated liposomes in a time-interval, which was called "accelerated blood clearance (ABC)" phenomenon. Such immunogenicity of PEGylated liposomes presents a barrier in the research of liposomal formulations and their use in the clinics. This review focused on the definition, the method of verification, the development of the reason for ABC phenomenon, influencing factors of ABC phenomenon, and discussed if other PEGylated nanocarriers also induce ABC phenomenon.

Objective To explore the true psychological experience of youthful patients with breast cancer in the period of diagnosis. Methods Using qualitative research based on employed phenomeno-logical methods,12 young patients of breast cancer were deep-interviewed, the literal data of interview were processed and analyzed by Colaizzid analysis method. Results 5 themes were found by analysis:change of self identification, serious uncertainty, change of self role, worry about working and social value and shortage of knowledge and ability for early diagnosis. Conclusions The clinical workers should under-stand the real psychological experience of young patients with breast cancer in diagnosis period and help them overcome the difficult period in their life.

Objective To understand the affecting factors of health and health promotion needs of one company staff so as to provide evidence for effective health intervention.Methods A convenience sampling method was used to enroll subjects in this cross-sectional study.All the participants were required to fill in a questionnaire to learn their demographics,health related factors and health promotion needs in the 2 work days of June 2012 had been studied.Chi-square test and unconditional logistic regression were used for data analysis.Results More males showed higher risk of disease than females (x2 =5.394,P ＜ 0.05).In conditional logistic regression,age was the main factor affecting health (odds ratio (OR) =3.127,95％ (confidence interval) CI 1.020-9.591,P ＜ 0.05) when gender,marital status,educational level and occupation were adjusted.Dietary nutritional knowledge (69.6％) and physical check-up (64.6％) were urgent needs among those surveyed.Conclusion Staff with varied demographic characteristics have different health related factors and physical check-up may provide a tool to meet their urgent needs of health promotion.

Objective To observe the effects of Buxu Huayu P rescription(BHP)on myeloid hematopoiesis in tumor -b ear-ing mice after combined chemotherap y.Methods Tumor -bearing mouse models were established by implanting P388tu-mor strain and the arrest of myeloid h ematopoiesis in the models was induced by intraperitoneal injection of c yclophos-phamide(CTX)and cytarabine(Ara -c ).The mice were allocated to 3groups:the normal control group,the model group and BHP group.Peripheral bloo d cell count,bone marrow karyocytes(BMK)count,colony count of progenitors o f granulocytes /monocytes(CCP -GM)and the proliferation of bone marrow cells(BMC)were examined.Results The combination of CTX and Ara -c could decrease plasma hemoglobin(Hb )content,the number of white blood cells(WBC),BMK and CCP -GM and the proliferatio n of BMC in model mice(P