Objective To find out the present situation of hepatitis B infection or immunity in Tianjin city,and to provide the scientific basis for the hepatitis B control and prevention.Methods 2 594 samples were selected with the methods of different proportionate stratified and cluster sampling,and the hepatitis B infection markers were detected by ELISA.Results The positive rates of HBsAg,anti-HBs,anti-HBc and HBV were 2.62％,46.72％,10.60％ and 51.54％.Conclusion Compared with the results in 1992,the positive rates of HBsAg,anti-HBc and HBV were decreased significantly,while the positive rate of anti-HBs increased significandy,which ascribed to the comprehensive measure with the vaccination against hepatitis B as a main strategy for control hepatitis B.

BACKGROUND:Previous studies have indicated that resistin stimulates a large set of chemokines in chondrocytes that are known to be important in inflammatory joint lesions.
OBJECTIVE:To further investigate the mechanism of co-regulation roles of transcription and post-transcription in the up-regulation of two chemokine genes CCL3 and CCL4 in chondrocytes in response to resistin.
METHODS:Human chondrocytes, T/C-28a2 and ATDC5 cels were cultured. The function of resistin on the chemokine genes, and the expression of C/EBPβ, nuclear factor-κB isoforms and chondrogenic specific miRNAs were tested by qPCR. The co-regulation of C/EBPβ and nuclear factor-κB was investigated by nuclear factor-κB inhibitor (IKK-NBD) and C/EBPβ inhibitor (SB303580) treatments, and subcelular localization was detected with or without resistin stimulation.
RESULTS AND CONCLUSION:Resistin could increase the expression of chemokine genes independently. Chondrocytes reacted in a non-restrictedly cel-specific manner to resistin; C/EBPβ inhibitor, nuclear factor-κB and some chondrogenic specific miRNAs in a combinatorial manner regulated chemokine gene expression. The activity of C/EBPβ was augmented by a transient increase in activity of nuclear factor-κB, and both transcription factors acted independently on the chemokine genes, CCL3 and CCL4.

Objective To investigate the prevalence of hepatitis C in Tianjin,China and major risk factors,and to provide a theoretical basis for the development of scientific prevention and control strategies for hepatitis C.Methods The monitoring system of notifiable infectious diseases was used to establish the database of hepatitis C cases reported from 2004 to 2014 in Tianjin.With reference to the results of serological surveillance and special case-control investigation,a statistical analysis was performed to investigate the epidemiological characteristics and prevalence of hepatitis C in Tianjin,as well as related risk factors.Results The report rate of hepatitis C in Tianjin showed a decreasing trend from 2004 to 2014 (x 2 =150.018,P ＜ 0.01).The group aged 60 years had the highest report rate (9.09/100000),and there were significant differences between this group and other groups (x2 =2540.887,P ＜ 0.01).The six urban districts in Tianjin had the highest report rate (6.54/100000),and there were significant differences between this area and other areas.The multivariate logistic regression analysis showed that a history of surgery (x2 =12.57,P ＜ 0.01),blood transfusion and blood products (x2 =36.67,P ＜ 0.01),hemodialysis (x2 =4.62,P ＜ 0.01),and intravenous drug use (x2 =12.92,P ＜ 0.01) were major risk factors for hepatitis C.Conclusion The epidemic situation of hepatitis C in Tianjin tends to decrease in recent years.Although there is no effective vaccine for hepatitis C,it still can be prevented and treated.The most effective interventions for hepatitis C include strengthening health education and raising the awareness rate of hepatitis C in the whole population,as well as early detection,diagnosis,and treatment.