1.Laparoscopic-assisted transcervical approach thyroidectomy
Wei SHENG ; Mingtao LI ; Weiqing LUO
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To explore the feasibility and reliability of laparoscopic assisted thyroidectomy. Methods A transversal 2.0 cm incision was made at the site 1.0 cm above the sternal notch. By using a harmonic scalpel under a 5 mm laparoscope, the thyroid glands and involving blood vessels were dealt with by either “coagulation-dissection-suction” technique or “dissection-coagulation-disconnection” technique to complete thyroid enucleation or lobectomy. Results There were 20 cases of adenoma (nodule) excision and 6 cases of unilateral lobectomy. The operation time was 80~130 min (mean, 100 min). The intraoperative blood loss was 10~40 ml (mean, 20 ml). No surgical complications occurred. Follow-up checkups in 23 cases for 1~)20 months (mean, 9 months) found a satisfactory cosmetic result and no recurrence. Conclusions Thyroidectomy under laparoscope is safe and reliable. This procedure offers a shorter incision, less invasion and better cosmetic results as compared with conventional thyroidectomy.
2.A comparative study between endoscopic and traditional thyroidectomy
Wei SHENG ; Weiqing LUO ; Mingtao LI
Chinese Journal of Minimally Invasive Surgery 2005;0(08):-
Objective To explore the safety and invasion of endoscopic thyroidectomy(ET).Methods A total of 156 cases of benign thyroid diseases were surgically treated from January 2003 to December 2005,including 64 cases of endoscopic thyroidectomy(Endoscopic Group) and 92 cases of traditional thyroidectomy(Traditional Group).The operation time,hemorrhage volume,length of incision,Visual Analogue Scale(VAS) scores for pain severity and cosmetic appearance,analgesic requirements,postoperative hospital stay,hospitalization costs,wound infection rate,and recurrent rate were compared between the two groups,respectively.Results The operation was successfully completed in both of groups.No hemorrhage,hoarseness,or hypocalcemia was encountered.Wound hydrops occurred in 4 cases in the Traditional Group.As compared with the Traditional Group,the Endoscopic Group had less blood loss(15?10 ml vs 20?15 ml;t=-2.330,P=0.021),lower VAS pain scores(2.5?1.0 vs(5.0?)1.6;t=-11.079,P=0.000),higher VAS cosmetic scores(8.0?1.5 vs 2.0?1.2;t=27.696,P=0.000),and less analgesic requirements(10 cases vs 28 cases;?~2=4.493,P=0.034),but longer operation time(80?20 min vs 70?30 min;t=2.330,P=0.021) and higher hospitalization costs(5 352.7?978 yuan vs 4 738.5?672 yuan;t=4.651,P=0.000).Follow-up observations for 2~30 months(mean,13 months) revealed recurrence in 2 cases in the Endoscopic Group and 3 cases in the Traditional Group.Conclusions As compared with traditional operation,endoscopic thyroidectomy has advantages of minimal invasion,early recovery,short hospitalization time,and high safety,being an ideal option for benign thyroid diseases.
3.Application of Ocular Vestibular Evoked Myogenic Potential and Caloric Test to Different Stages of Meniere Disease
Wenjun FAN ; Bin LUO ; Ruirui GUAN ; Weiqing XU
Journal of Audiology and Speech Pathology 2017;25(5):488-491
Objective To investigate the characteristics and clinical significance of ocular vestibular evoked myogenic potential (oVEMP) and caloric test in Meniere disease (MD) at different hearing stages.Methods Fifty-five patients(52.8±15.8 years old) with MD were divided into stage 1(9 cases,48.8±13.8 years old), stage 2(9 cases,46.0±16.3 years old), stage 3(23 cases,50.3±13.5 years old) and stage 4(cases 14, 53.5±16.2 years) respectively according to the pure tone audiometry.They were evaluated by oVEMP and caloric test.Results The abnormal rates of oVEMP were 55.6%, 66.7%, 78.3%, 78.6%,and caloric tests were 22.2%, 33.3%, 78.3%,and 85.7% respectively in stage 1, 2, 3, and 4 MD patients.The amplitudes of oVEMP in stage of 1, 2, 3, and 4 MD patients were 4.3±4.0 μV,3.5±2.3 μV,2.5±2.4 μV,and 1.3±0.5 μV,respectively.Conclusion The abnormal rates of oVEMP and caloric tests in MD patients increased with the degree of hearing impairment and the amplitudes of oVEMP were decreased, suggesting that utricle and horizontal semicircular canal injuries were aggravated.
4.Methylation State within MAGE-1 B′B Promoter in Gastric Carcinoma
Lin XU ; Bing LUO ; Qing WANG ; Hefei XU ; Weiqing HUANG ; Hua LIANG ;
Chinese Journal of Cancer Biotherapy 1994;0(01):-
Objective: To study the methylation state within MAGE 1 B′B promoter in gastric carcinoma and the association between demethylation and pathological differentiation, the association between demethylation and clinical stage. Methods: Using methylation sensitive restriction analysis followed by polymerase chain reaction (PCR),we studied 80 specimen that were obtained from surgical samples (including 40 gastric carcinoma and 40 matched adjacent normal gastric mucosae).Results: An demethylation state was identified in DNA from gastric carcinoma specimens.The demethvlation rate is 25%(10/40).In contrast,no demethylation state was identified in DNA from matched adjacent normal gastric mucosae. The differences were Significant statistically. ln our study, the demethylation in poorly, moderately, and well differentiated glandulous cell carcinoma were detected at frequencies of 50%,18.7% and 8.3%,respectively, The differences were significant statistically ( P
5.Clinical evaluation of rapid prenatal detection of aneuploid abnormalities with multiplex ligationdependent probe amplification
Caiqun LUO ; Jiansheng XIE ; Weiqing WU ; Hui YUAN ; Zhiyong XU ; Fuwei LUO ; Qian GENG ; Huakun ZHANG ; Ying HAO ; Hong LIU
Chinese Journal of Laboratory Medicine 2012;35(2):160-164
Objective To estimate clinical application of multiplex ligation-dependent probe amplification (MLPA) for rapid prenatal detection of aneuploid abnormalities in amniotic fluid.Methods Totally 1229 amniotic fluid samples were collected from the pregnant women receving prenatal diagnosis for chromosomal abnormalities in Prenatal Diagnosis Center of Shenzhen Maternity and Child Healthcare Hospital from October 2009 to December 2010.All the samples were investigated independently with both MLPA and G-band karyotyping to detect aneuploidies of chromosomes X,Y,13,18 and 21.A comparison was followed the results acquired from two methods for evaluation of sensitivity and specificity of MLPA.ResultsThirtyeight aneuploidies were detected by G-band karyotyping,in which 34 were nonmosaic aneuploidies and 4were mosaic aneuploidies.MLPA and G-band karyotyping had consistent results in detecting the nonmosaic aneuploidies of chromosomes X,Y,13,18 and 21. Among 4 mosaic aneuploidies detected by G-band karyotyping,2 were confirmed by MLPA independently.Conclusions The sensitivity and specificity of MLPA in detecting the nonmosaic aneuploidies of chromosomes X,Y,13,18 and 21 were clinically acceptable.MLPA provides an efficient,reliable method for rapid detection of aneuploidies.
6.Non-islet cell tumor-induced hypoglycemia due to overproduction of insulin-like growth factor-Ⅱ——two case reports and literature review
Jieli LU ; Yongju ZHAO ; Jinfeng TANG ; Jianming LIU ; Weiqing WANG ; Wei ZHANG ; Yanyun GU ; Bangyao LUO ; Xiaoying LI ; Guang NING
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
The relationship between abnormal circulating GH-insulin-like growth factor (IGF) axis and hypoglycaemia was explored in 2 cases of non-islet cell tumor associated hypoglycaemia (NICTH). Serum level of IGF-Ⅱ was increased, the levels of GH, IGF-Ⅰ, IGFBP-3 were decreased to some extent, and IGF-Ⅱ/IGF-Ⅰ (molar ratio) was significantly increased in these two cases. In one case, circulating GH-IGF axis returned to normal after complete removal of the tumor. In the diagnosis of NICTH, the abnormal circulating GH-IGF axis is quite valuable and IGF-Ⅱ/IGF-Ⅰ appears to be a more sensitive parameter.
7.Mutation analysis of the androgen receptor gene in a complete androgen insensitivity syndrome family.
Weiqing WU ; Fuwei LUO ; Qian GENG ; Ying HAO ; Wubin CHEN ; Jun CAI ; Jiansheng XIE
Chinese Journal of Medical Genetics 2009;26(6):606-609
OBJECTIVETo study the mutation of the androgen receptor gene in a family with complete androgen insensitivity syndrome and to explore the pathogenicity of the mutation.
METHODSPCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls; conservation of the mutation site was analyzed by comparison of the sequence of amino acid among different species.
RESULTSThe DNA sequence of the three patients contained the same substitution of a single nucleotide on codon 681 GAG to GAT of exon 4, which located in the ligand binding domain of the AR receptor and led to substitution of glutamic acid to aspartic acid in the AR receptor. Their mother was heterozygote of E681D. E681D was not observed in the normal controls. The E681 site was extremely conservative in different species.
CONCLUSIONThe E681D mutation of the AR gene is a novel mutation of leading to complete androgen insensitivity syndrome.
Adult ; Amino Acid Sequence ; Androgen-Insensitivity Syndrome ; genetics ; Animals ; Base Sequence ; DNA Mutational Analysis ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Mutation ; Pedigree ; Receptors, Androgen ; chemistry ; genetics ; Sequence Alignment ; Young Adult
8.A novel mutation of cartilage oligomeric matrix protein gene underlies multiple epiphyseal dysplasia.
Hui WANG ; Jiansheng XIE ; Weiqing WU ; Zhiyong XU ; Fuwei LUO ; Qian GENG
Chinese Journal of Medical Genetics 2013;30(3):322-325
OBJECTIVETo perform mutation analysis for a female with multiple epiphyseal dysplasia (MED) and provide pre-symptomatic and prenatal diagnosis.
METHODSMutation screening of cartilage oligomeric matrix protein (COMP) gene was carried out through targeted next-generation DNA sequencing and Sanger sequencing.
RESULTSA novel c.956 A>T resulting in substitution of Aspartic acid 319 for Valine (p.Asp319Val) has been identified in exon 9 of the COMP gene in the patient. As predicted by a SIFT software, above mutation can cause damage to the structure of COMP protein.
CONCLUSIONA novel c.956 A>T substitution mutation has been identified in a patient featuring MED.
Adult ; Base Sequence ; Cartilage Oligomeric Matrix Protein ; Exons ; Extracellular Matrix Proteins ; genetics ; Female ; Glycoproteins ; genetics ; Humans ; Matrilin Proteins ; Mutation ; Osteochondrodysplasias ; diagnosis ; genetics ; Polymorphism, Single Nucleotide ; Sequence Alignment
9.Application of array-CGH and MLPA for detection of 4 cryptic unbalanced translocations.
Qian GENG ; Weiqing WU ; Fuwei LUO ; Zhiyong XU ; Wubin CHEN ; Fang LI ; Jiansheng XIE
Chinese Journal of Medical Genetics 2013;30(3):288-292
OBJECTIVETo use array comparative genomic hybridization (array-CGH) and multiplex ligation-dependent probe amplification (MLPA) to detect unbalanced rearrangements in 4 cases suspected to have chromosome disease but were undetected with conventional karyotype analysis, and to assess the applicability of array-CGH and MLPA for detection of unbalanced translocation.
METHODSGenomic DNA was extracted with standard procedures. All cases were analyzed by array-CGH and subtelomeric MLPA.
RESULTSAll of the cases were identified to have unbalanced translocations by array-CGH analysis, among which 3 were consistent with subtelomeric MLPA analysis. For the remaining one, its chromosomal abnormality was not detected by MLPA as the imbalance has occurred outside of target regions.
CONCLUSIONBoth array-CGH and MLPA techniques can complement conventional karyotyping for detecting unbalanced translocations. The combination features both high resolution and efficiency for clinical use.
Adult ; Child ; Chromosome Deletion ; Chromosome Duplication ; Comparative Genomic Hybridization ; Humans ; Infant ; Karyotyping ; Male ; Multiplex Polymerase Chain Reaction ; Phenotype ; Translocation, Genetic
10.Associations between maternal parenting styles and preschool children s quality of life in Chengdu
LI Chunrong, LUO Shengyu, LIN Li, CHEN Weiqing, REN Yan, ZHANG Meng, GUO Yawei
Chinese Journal of School Health 2023;44(1):81-84
Objective:
To investigate the association between maternal parenting styles and quality of life among preschools,to provide a scientific theoretical basis for interventions targeting at prmoting early life health.
Methods:
From May to July, 2021, a stratified cluster sampling method was used to enroll 4 233 child mother dyads from 14 preschools in Chengdu. An online questionnaire survey was administered to collect socio demographic information, maternal parenting styles, and children s quality of life.
Results:
The overall score of quality of life was (80.17±9.81) among preschool children in Chengdu. In the multivariate linear regression models, maternal emotional warmth were significantly associated with higher scores in emotional functioning, social functioning, school functioning, psychosocial health summary score, and total scores ( β coefficients in the high level group were 2.63 , 4.95 , 12.05, 6.54,4.88, P <0.05). In contrast, both maternal rejection and overprotection were significantly associated with lower scores in physical functioning, emotional functioning, social functioning, school functioning, psychosocial health summary score, and the total scores of children s quality of life (for rejection: β coefficients in the high level group were -9.39, -10.82, -7.12 , -6.04 , -8.00, and -8.35 , respectively; for overprotection: β coefficients in the high level group were -6.71, -5.85, -3.08 , -2.39 , -3.77, and - 4.51 , respectively, P <0.05). The associations between high level of maternal rejection and children s emotional functioning showed significant gender differences( β =-3.23, P <0.05).
Conclusion
Maternal parenting style has a significant impact on children s quality of life. Interventions targeting at maternal parenting styles may be beneficial to improve the quality of life in preschool children.