190-kilodalton glycoprotein (P190) of Plasmodium falciparum. precursor of the major surface protein of merozoites, is considered a promising candidate for blood stage malarial vaccine. We designed six primers according to the sequence of MAD20 strain, with a GC clamp and BamHI site at the 5'- end of each one, and a GC clamp and Xbal site at the 3'- end of each one. The primers were synthesized by phosphoramidite approach (User's Manual of ABI Company) and purified using HPLC. Three fragments in the second, third and fourth conserved regions of P190 gene of Plasmodium falciparum FCC1/HN strain isolated from the blood of patients in Hainan Province of China were amplified by the polymerase chain reaction (PCR) technique. The amplified fragments were subcloned into pUC18 vectors and sequenced using the dideoxy chain termination method. All three regions of P190 gene of FCCl/HN strain also were highly conservative as compared with P190 gene of MAD20 (Papua New Guinea isolate), K1 (Thailand isolate), Wellcome (West Africa isolate) and CAMP (Malaysia) strains of Plasmodium falciparum. The C at position 81 in the second conserved block of P190 gene of FCC1/HN isolate was substituted by T, which did not change the amino acid determined by the coden corresponding to the substitution.

Objective To understand the Shanghai City General Hospital in two non Department of Endocrinology nurses′ comprehensive intervention of diabetes care ability and its influencing factors. Methods To investigation and analysis of the result of comprehensive nursing intervention in diabetic ability questionnaire to two hospitals in Shanghai city of Pudong New Area in a non Department of Endocrinology nurses. Results The comprehensive nursing intervention of non Department of Endocrinology nurses diabetes score was (46.75 ± 7.91) points, diabetic blood glucose monitoring knowledge score was (14.76 ± 2.96) points, insulin injection knowledge score was (20.28 ± 4.83) points, diabetes knowledge score was (11.71±1.55) points. Multivariate analysis showed that the number of years of work and attention to diabetes were the main factors affecting their scores. Conclusions The comprehensive nursing intervention ability of non Endocrinology Department of diabetes care at a moderate level, the ability to provide comprehensive nursing intervention for the patients need to be further improved, the need to strengthen the skills training and diabetes specialist.

Objective: To investigate whether ω-3 fatty acid supplementation affects immune function, infection and surgery ratio in severe acute pancreatitis patients. Methods: Fifty-six severe acute pancreatitis patients were prospectively enrolled and were randomly assigned to receive total parenteral nutrition (TPN) for 5 days in a double-blinded manner with either soybean oil (SO) or fish oil (FO) supplementation. The level of IL-10 and HLA-DR expression were measured before TPN and on the sixth day after starting TPN. The clinical outcomes such as infection and surgery ratio were recorded. Results % Patients treated with ω-3 FA had significantly higher IL-10 concentration and better HLA-DR expression during the observation period. Although there was no significant difference between two groups, the ratio of infection and surgery ratio had a trend to decrease in the study group. Conclusion: TPN supplemented with ω-3 FA improves anti-inflammatory cytokine and immune function in SAP patients. It also leads to the attenuated risk of infection and surgery ratio.

Objective To investigate the results of follow-up visits of pregnancy course, delivery and infants of women who got clinically pregnant by assisted reproductive technique after gonadotropin-releasing hormone agonist (GnRH-a) added for luteal support, and to analyse the influence of adding GnRH-a in luteal support on the safety of mother and infant. Methods A retrospective analysis was carried out on the medical record from 215 patients who got clinically pregnant after luteal phase long regimen fresh-cycle transfer was operated. According to the differences in luteal support methods, the patients were assigned to Group A (124 patients, progesterone+dydrogesterone group), Group B (91 patients, GnRH-a added group). The patients′ pregnancy course, delivery time, and the growth and development of infants within 1-2 years were followed up. Results (1)There was no obvious difference between Group A and Group B in terms of the abortion ratio during the early pregnancy (8.1%, 12.1%), the rate of abortion villous deformity (50.0%, 9.1%), the rate of heterotopic pregnancy (10.5%, 5.5%) and rate of twin pregnancy (19.4%, 28.6%;all P>0.05).(2)Compared to group A, during the middle and late pregnancy of single or twin pregnancy in Group B , there was no obvious difference in the rate of fetal chromosomal abnormality, organ malformation incidence, late abortion rate and stillbirth rate (all P>0.05).(3)As to childbirth, in the case of twin pregnancy, there was a higher rate of premature delivery (60.0%, 39.1%;P=0.041), as well as rate of lower birth weight of newborn (56.0%, 34.8%; P=0.037) in group B.(4)The statistics on general growth and development as well as infantile common diseases within 2 years after birth indicated that there was no obvious difference between the two groups in single birth and twin birth subgroup (all P>0.05). Conclusion On the basis of controlling of implanted embryos and reducing the occurrence of twins, GnRH-a luteal support maybe relatively safe and effective.