Objective To explore the roles and clinical significance of glutamine(GLN) in carbon tetrachloride(CCl_(4)) induced acute hepatic injury.Methods The SD rats were divided into model group,GLN pretreated group and control group.The animal model was established by CCl_(4) intraperitoneal injection.GLN at dose of 1 g/kg was intragastrically administrated for 7 d before intraperitoneal injection of CCl_(4) in the rats of GLN pretreated group.The rats were executed 4,8,12,16,24 h after injure.To evaluate the hepatic injury,the serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected by automatic biochemistry analysator.The liver tissue was observed by light microscope and transmission electron microscope(TEM).The expression of nuclear factor-?B(NF-?B) was detected by immunohistochemistry and the tumor necrosis factor?(TNF-?) was detected by reverse transcription polymerase chain reaction(RT-PCR).The apoptosis of hepatocyte was detected by TUNEL.The oncosis index was detected by TEM.Results The levels of ALT,AST,NF-?B,TNF-? mRNA in model group were apparently elevated as compared to control group(P

Epithelial-mesenchymal transition is the phenomenon of epithelial cells transforming to mesenchymal cells under special microenvironment,and thus tumor cells obtain characters such as difficult to adhere,motility enhancement,resistant to apoptosis.In the process of embryonic growth and tumor development,epithelial cells were found to be transformed into mesenchymal cells.Many researches showed that epithelial-mesenchymal transition plays an important role during the invasion and metastasis of colorectal cancer,and its mechanism involves many kinds of signaling pathways and related molecules.

Male Sprague~Dawley rats were pretreated with phenobarbital and randomly divided into 6 groups and were exposed to O2,/N2/l. 2 MAC anesthetics for I hr; NC, 21 % O2/79% N2; HC, 14 %O2,/86%N2; NH, 21 %O2/79% N2/l. 2 MAC halothane;HH, 14 %O2/86 %N2/ 1. 2 MAC Halothane; NS, 21 %O2/79%N2/ 1. 2 MAC sevoflurane; HS, 14 %O2/86 %N2/ 1. 2 MAC sevoflurnae. Liver specimens andblood were taken 24 hrs after exposure. Thenecrosls and denaturatlon of hepatocellularwere quantltatlvely estlmated by stereoscopy.Ultrastructural morphology was analysed by computer. The liver ofall rats given halothane (14%O2) had extensive centrilobu- lar necrosls and denaltlration. There were an increase in serum glutamic pyruvic transmi- nase accompnaying the morphologic damage。No marked hepatotoxicity was foundin the rats following sevoflurane expoure compared with controls. Hypoxia was the main cause of swelling of mltochondria. Results suggest that sevoflurane has less hepattc injtry than holotnane.

Objective To evaluate the prevention and treatment of rectal complications following 125I brachytherapy for prostate cancer. Methods A total of 90 patients with locally advanced prostate cancer was treated by transrectal ultrasound guided prostate 125I brachytherapy combined with maximal androgen blockage from November 2001 to June 2006. The patients were followed every 1～3 months postoperatively, and rectal complications was analyzed and treated. Results Eighty-nine patients were followed for 1～55 months (mean, 22 months). The incidences of rectal complications at grade Ⅰ, Ⅱ, Ⅲ, and Ⅳ were 11.2% (10/89), 6.7% (6/89), 0%, and 2.2% (2/89), respectively. Symptomatic therapy was conducted in 16 patients with grade Ⅰ or Ⅱ complications, which were symptomatically relieved 6 months later. The grade Ⅳ complications occurred in 2 patients, one of whom underwent repeated examinations and treatment for proctitis, and the other of whom was complicated with severe diabetes and received a laparocolostomy with cystostomy. Conclusions The optimal medical treatment for radiation proctitis is follow-up checkups and observations. To reduce the incidence of urethrorectal fistula, excessive examinations and treatment should be avoided. Accompanying diabetes should be treated promptly and actively.

Objective To investigate the technological feasibility and short-term efficacy of 125I seeds implantation in head and neck cancer. Methods Thirty patients with head and neck recurrent cancer were treated with 125I seeds interstitial brachytherapy. The operation was performed under local anesthesia and the 125I seeds were implanted under the guidance of ultrasonography. The seed needles were implanted parallelly to each other, at 1～1.5 cm apart. The Mick applicator was used to implant seed at 1 cm interval. The radioactive activity per seed ranged 0.40～0.70 mCi, and the matched peripheral dose (MPD) was 90～160 Gy. The number of 125I seed implanted per lesion was 3～84 (median, 24). Ten patients with a seed radioactive dose

[Objective] To evaluate the effect and safety of bee-stinging therapy in treating undifferentiated connective tissue disease (UCTD). [Methods] Forty cases of UCTD were treated with bee-stinging therapy (qd or qod) for two treatment course, each course lasting 15 times. The symptoms of morning stiffness (MS), joint pain (JP) and articular swelling (AS), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) and side effects were observed before and after treatment. [Results] After treatment, clinical symptoms and ESR and CRP were significantly improved (P

Objective To assess the changes of left ventricular function after off-pump coronary artery bypass (OPCAB) using Tei index. Methods Fourty-six patients of coronary artery disease, who had accepted OPCAB, were enrolled. Left ventricular ejection fraction(LVEF), E/A ratio at mitrial orifice(E/A), E deceleration time(EDT), isovolumic relaxation time(IVRT), ratio of systolic wave to diastolic wave of pulmonary vein flow(S/D) and Tei index were measured by transthoracic echocardiography before, immediate, 1 week, 1 month, 3 months and 6 months after OPCAB. Results Tei index decreased gradually as time went on after OPCAB and the differences were significant between different times(P

Objective To evaluate the diagnostic value of MSCT in abdominal aortic aneurysm(AAA).Methods 26 patients suspected AAA and admitted to our hospital were studied and checked by MSCT prospectively.The accuracy and features of MSCT were analyzed. Results 26 patients were diagnosed as AAA by MSCT,in which 17 cases were true AAA,and 9 were false AAA.MSCT for true AAA patients showed fusiform dilatation of abdominal aorta bulging to the left,and showed visible thrombus and calcification in aneurysm wall of residual tumor around the cavity when aneurysm ruptured.MSCT of false AAA showed aortic eccentric enlargement lump,which could be outside the boundaries of vascular calcification.25 cases of AAA were surgically confirmed,and the coincidence rate of MSCT and pathology was as high as 96.1% (25/26).Arterial wall calcification was found in all patients,in which 17 cases had mural thrombus.Conclusion MSCT is a safe,effective and non-invasive diagnostic technology for early detection and treatment of AAA, which may help determine patient’s condition and guide the treatment.

Purpose To observe the characteristics of coronary artery lesion in patients with unstable angina pectoris (UAP) and stable angina pectoris (SAP) using virtual histology intravascular ultrasound (IVUS-VH).Materials and Methods A total of 199 patients with angina pectoris were enrolled and divided into SAP group (101 cases) and UAP group (98 cases) according to clinical symptoms, ECG and myocardial enzyme level. All the patients underwent coronary angiography to determine the criminals vessels, grayscale intravascular ultrasound was used to measure and compare the external elastic membrane area (EEMA), lumen cross-sectional area (lumen CSA), plaque area (PA), plaque burden (PB), remodeling index (RI), plaque eccentricity index (EI) of the criminals vessels. IVUS-VH method was used to measure and compare the area and percentage of calcified tissue,fibrous tissue, lipid tissue and necrotic tissue in the plague components of the criminals vessels between the two groups.Results There was no significant difference (t=1.392,-0.345, 1.921, 0.378 and 0.857,P>0.05) of EEMA, lumen CSA, PA, EI and RI measured at the location with smallest lumen area between the two groups. Necrotic core area and percentage of lesion composition in UAP group was significantly higher than that in SAP group (t=2.361,P<0.05). There was no statistically significant difference (t=1.045, 1.884 and 0.787,P>0.05) between the two groups on the area of fiber, lipid and calcification. On the distribution of plaque components, fibrous plaque area percentage of UAP group was lower than that in SAP group (t=-2.418, P<0.05), while the necrotic core area was significantly higher than SAP group (t=2.602, P<0.05), there was no significant difference (t=-0.551 and 0.085,P>0.05) between the two groups on lipid and calcification area.Conclusion Necrotic core area and percentage of plaque composition in the UAP group is larger than the other group. Criminals lesions are more unstable and more easily to be complicated with acute cardiovascular events.

Background:Detection and removal of colorectal polyp by colonoscopy is of great importance for prevention of colorectal carcinoma. Aims:To investigate whether the clinical symptoms of patients undergoing colonoscopy may hint colorectal polyp and carcinoma,and provide reference for candidate selection in colonoscopic screening. Methods:A total of 2 366 patients undergoing colonoscopy were recruited and the history information such as symptoms at outpatient visits, site and nature of the lesions was collected for analyzing the detection rates of colorectal polyp and carcinoma and the correlations of clinical symptoms with the risk and site of the disease. Results:The overall detection rates of colorectal polyp and carcinoma were 20. 5% and 5. 4% ,respectively,in 2 366 patients. The detection rates were significantly higher in symptomatic patients than those in asymptomatic patients(24. 2% vs. 4. 5% for polyp and 6. 4% vs. 0. 9% for carcinoma,P all = 0. 000). Moreover,when patients were classified by major symptoms,the detection rate of colorectal polyp was significantly increased in patients with diarrhea(OR = 1. 213),hematochezia(OR = 2. 076),and changing of stool consistency(OR = 1. 503)(P all < 0. 05),and the detection rate of colorectal carcinoma was significantly increased in patients with abdominal pain( OR = 1. 568),hematochezia( OR = 2. 837),changing of stool consistency( OR =2. 206),and tenesmus( OR = 1. 735)( P all < 0. 05). The major symptoms being hematochezia,changing of stool consistency and tenesmus were associated with lesions locating at rectum or left hemicolon(P all < 0. 05). Conclusions:Diarrhea, hematochezia and changing of stool consistency hints risk for colorectal polyp, while abdominal pain, hematochezia,changing of stool consistency and tenesmus hints risk for colorectal carcinoma. Colonoscopy is strongly recommended for patients with these symptoms.